Rh1DG230900

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000446
Physical Location & Seq
Forward (+)
44399925 .. 44407830
7906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG230900.1

Sequence Viewer

Length: 1290 bp
ATGAGGGAAGACAGTGCTGAAGAACTGCAGTTTTATAGTTACTGGTATTATGCAGAAGTTGTGATTCCAACCAGGCAGGAAGCTAAAATGGCCAGATTGCAATTCCGTGATGCCAATGAGGATGATGTACCATCCGAGGAACTCCCACATCTCCTACTCATTCATCCACCATCATCCTTCACACGTCTCCCATCTGAAATCTGTGAAAAGTTCCGTGTCCTGAAAGCATGGGAGTCGCCACTCCCTCTAGACCAGTTCCTGATCACCCATGCATGCTCAATCCAAGCCCTACTCATTCGCGGTTTTCTTATGAATAACACAGACATCCTTCAGCAGCTACCGGCACTGAAACTCATCATAACAATCAGCGTTGGTGTGAACCACATTGACATGGCAGAGTGCCGGCAGCGTGGGATTTCTGTGACCAATGCGGGAAGTGTATACTCGGAAGATGTTGCTGATATTGCTGTTGGGTTGTACATTGATGTGCTTAGAAAACTTTCAGCAGCAGACCGAATCAATGAGCAAGAAAAGATGAGATCAGCAAACACAGGTATGGAGGTGGCGAGGTCATCGGAATATTTGCAAAGGCAGGACCTGGCCGTCGTCCCCAAGCCTGATGCCACCAAGGCGGAAGAACCAAAGGCTCGATGGCCTTATATCAGCAATTTACCTCATGGTAGGACGATCATAATCTCAAGGAAGAACCCACCTACTGAGCGTCCTTGCGCCACTTATACCGAGATGGCCCCCCGCCTCTCCGACCCTGGTGGGAAGCGAGTTGGGATTGTTGGATTAGGAAGCATTGGCTCAGAAGTTGCTAAACGACTTGAGGCCTTTGGCTGCATCATCTCATACAATTCAAGGAGGGAAAAGCCAACTTCGCCATACCCCTTCTATTCTATCATATCTGAACTTGCAGCCAATAGTGATGCCCTCATCATCTGTTGTGCATTAACAGAACAAACCCACCATATGATTAACAAGGAAGTCTTGGCTTCACTGGGAAGAGAGGGTGTGATTGTTAATGTAGGCCGCGGGGCAATAATTGATGAGAAAGAACTGGTTAAGTGTTTGGTGAGTGGGGAAATAAGAGGTGCTGGTTTTGATGTGTTTGAGAATGAGCCTCAAGTTCCGGAAGAGCTCTTTACATTGGACAATGTTGTTCTATCCCCACATACGGCTATTTTTACTCCGGAATCTTTGCAGAGTGTGAAAGAAGTACTAATCGCCAATGTGGAAGCTTTCTTCTTGAATAAACCTCTGGTTACTCCTGTCATGGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

429

Amino Acids

47.79

Weight (kDa)

5.21

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 87 - 426 1.2e-18 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 251 - 395 3.3e-45 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 602
AccI GTMKAC 1 cut(s) 441
AccII CGCG 2 cut(s) 300, 1038
AccIII TCCGGA 2 cut(s) 1135, 1195
AciI CCGC 6 cut(s) 300, 431, 632, 754, 1036, 1038
AcoI YGGCCR 2 cut(s) 90, 600
AcuI CTGAAG 2 cut(s) 39, 314
AfaI GTAC 3 cut(s) 129, 479, 1224
AfiI CCNNNNNNNGG 1 cut(s) 1180
AflIII ACRYGT 1 cut(s) 182
AgsI TTSAA 2 cut(s) 864, 1255
AjiI CACGTC 1 cut(s) 185
AjnI CCWGG 3 cut(s) 71, 597, 766
AluBI AGCT 4 cut(s) 83, 337, 1144, 1244
AluI AGCT 4 cut(s) 83, 337, 1144, 1244
Alw21I GWGCWC 1 cut(s) 1146
Alw26I GTCTC 1 cut(s) 191
AlwNI CAGNNNCTG 2 cut(s) 259, 598
Aor13HI TCCGGA 2 cut(s) 1135, 1195
AoxI GGCC 6 cut(s) 90, 600, 653, 747, 834, 1033
ApeKI GCWGC 5 cut(s) 334, 406, 506, 843, 920
Asp700I GAANNNNTTC 1 cut(s) 499
AspLEI GCGC 1 cut(s) 731
AspS9I GGNCC 2 cut(s) 595, 748
AsuHPI GGTGA 2 cut(s) 256, 1090
AvaII GGWCC 1 cut(s) 595
BalI TGGCCA 1 cut(s) 92
BanII GRGCYC 1 cut(s) 1146
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 1146
BbvI GCAGC 5 cut(s) 346, 418, 518, 830, 932
BccI CCATC 5 cut(s) 139, 178, 199, 645, 739
BceAI ACGGC 2 cut(s) 587, 1197
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 3 cut(s) 73, 599, 768
BclI TGATCA 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 26
BglI GCCNNNNNGGC 1 cut(s) 629
BisI GCNGC 6 cut(s) 335, 407, 507, 844, 921, 1036
BlsI GCNGC 6 cut(s) 336, 408, 508, 845, 922, 1037
BmcAI AGTACT 1 cut(s) 1224
Bme1390I CCNGG 3 cut(s) 73, 599, 768
Bme18I GGWCC 1 cut(s) 595
BmgBI CACGTC 1 cut(s) 185
BmgT120I GGNCC 2 cut(s) 595, 748
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 3 cut(s) 73, 599, 768
BmrI ACTGGG 1 cut(s) 1013
BmsI GCATC 4 cut(s) 100, 610, 855, 922
BmuI ACTGGG 1 cut(s) 1013
BpiI GAAGAC 1 cut(s) 15
BpuEI CTTGAG 3 cut(s) 682, 851, 1113
BsaBI GATNNNNATC 1 cut(s) 692
BsaJI CCNNGG 4 cut(s) 135, 627, 766, 1036
BsaWI WCCGGW 2 cut(s) 1135, 1195
Bsc4I CCNNNNNNNGG 1 cut(s) 1180
Bse118I RCCGGY 2 cut(s) 340, 402
Bse1I ACTGG 4 cut(s) 47, 253, 1008, 1068
Bse8I GATNNNNATC 1 cut(s) 692
BseAI TCCGGA 2 cut(s) 1135, 1195
BseBI CCWGG 3 cut(s) 73, 599, 768
BseDI CCNNGG 4 cut(s) 135, 627, 766, 1036
BseGI GGATG 6 cut(s) 127, 131, 163, 173, 324, 1288
BseJI GATNNNNATC 1 cut(s) 692
BseLI CCNNNNNNNGG 1 cut(s) 1180
BseMII CTCAG 2 cut(s) 708, 825
BseNI ACTGG 4 cut(s) 47, 253, 1008, 1068
BseXI GCAGC 5 cut(s) 346, 418, 518, 830, 932
Bsh1236I CGCG 2 cut(s) 300, 1038
BshFI GGCC 6 cut(s) 92, 602, 655, 749, 836, 1035
BsiHKAI GWGCWC 1 cut(s) 1146
BsiSI CCGG 4 cut(s) 341, 403, 1136, 1196
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 1 cut(s) 1180
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 6 cut(s) 92, 602, 655, 749, 836, 1035
Bsp1286I GDGCHC 1 cut(s) 1146
Bsp13I TCCGGA 2 cut(s) 1135, 1195
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 3 cut(s) 261, 539, 687
BspACI CCGC 6 cut(s) 300, 431, 632, 754, 1036, 1038
BspANI GGCC 6 cut(s) 92, 602, 655, 749, 836, 1035
BspCNI CTCAG 2 cut(s) 709, 824
BspEI TCCGGA 2 cut(s) 1135, 1195
BspFNI CGCG 2 cut(s) 300, 1038
BspLI GGNNCC 1 cut(s) 750
BspMAI CTGCAG 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 1134
BsrFI RCCGGY 2 cut(s) 340, 402
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 4 cut(s) 47, 253, 1008, 1068
BssAI RCCGGY 2 cut(s) 340, 402
BssECI CCNNGG 4 cut(s) 135, 627, 766, 1036
BssMI GATC 3 cut(s) 261, 539, 687
BssNAI GTATAC 1 cut(s) 442
BssT1I CCWWGG 1 cut(s) 627
Bst1107I GTATAC 1 cut(s) 442
Bst2UI CCWGG 3 cut(s) 73, 599, 768
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 2 cut(s) 1003, 1134
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 274, 404
BstDEI CTNAG 3 cut(s) 491, 717, 811
BstDSI CCRYGG 1 cut(s) 1036
BstF5I GGATG 6 cut(s) 127, 131, 163, 173, 324, 1288
BstFNI CGCG 2 cut(s) 300, 1038
BstHHI GCGC 1 cut(s) 731
BstKTI GATC 3 cut(s) 264, 542, 690
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 3 cut(s) 261, 539, 687
BstMWI GCNNNNNNNGC 4 cut(s) 89, 464, 629, 883
BstNI CCWGG 3 cut(s) 73, 599, 768
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 3 cut(s) 71, 597, 766
BstSFI CTRYAG 1 cut(s) 26
BstUI CGCG 2 cut(s) 300, 1038
BstV1I GCAGC 5 cut(s) 346, 418, 518, 830, 932
BstV2I GAAGAC 1 cut(s) 15
BstZ17I GTATAC 1 cut(s) 442
BsuRI GGCC 6 cut(s) 92, 602, 655, 749, 836, 1035
BtgI CCRYGG 1 cut(s) 1036
BtrI CACGTC 1 cut(s) 185
BtsCI GGATG 6 cut(s) 127, 131, 163, 173, 324, 1288
BtsIMutI CAGTG 3 cut(s) 19, 344, 1001
Cac8I GCNNGC 2 cut(s) 274, 404
CaiI CAGNNNCTG 2 cut(s) 259, 598
CfoI GCGC 1 cut(s) 731
Cfr10I RCCGGY 2 cut(s) 340, 402
Cfr13I GGNCC 2 cut(s) 595, 748
Cfr42I CCGCGG 1 cut(s) 1039
CseI GACGC 1 cut(s) 710
Csp6I GTAC 3 cut(s) 128, 478, 1223
CviAII CATG 6 cut(s) 228, 269, 273, 391, 677, 1279
CviQI GTAC 3 cut(s) 128, 478, 1223
DdeI CTNAG 3 cut(s) 491, 717, 811
DpnI GATC 3 cut(s) 263, 541, 689
DpnII GATC 3 cut(s) 261, 539, 687
DrdI GACNNNNNNGTC 1 cut(s) 602
DseDI GACNNNNNNGTC 1 cut(s) 602
EaeI YGGCCR 2 cut(s) 90, 600
Eam1104I CTCTTC 2 cut(s) 1003, 1134
EarI CTCTTC 2 cut(s) 1003, 1134
EciI GGCGGA 1 cut(s) 647
Ecl136II GAGCTC 1 cut(s) 1144
Eco130I CCWWGG 1 cut(s) 627
Eco147I AGGCCT 1 cut(s) 836
Eco24I GRGCYC 1 cut(s) 1146
Eco47I GGWCC 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 1144
Eco57I CTGAAG 2 cut(s) 39, 314
EcoICRI GAGCTC 1 cut(s) 1144
EcoO109I RGGNCCY 1 cut(s) 595
EcoRII CCWGG 3 cut(s) 71, 597, 766
EcoT14I CCWWGG 1 cut(s) 627
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 1146
ErhI CCWWGG 1 cut(s) 627
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 6 cut(s) 231, 272, 276, 394, 680, 1282
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FaqI GGGAC 1 cut(s) 593
FatI CATG 6 cut(s) 227, 268, 272, 390, 676, 1278
FauI CCCGC 3 cut(s) 424, 761, 1031
FauNDI CATATG 1 cut(s) 975
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 6 cut(s) 335, 407, 507, 844, 921, 1036
FokI GGATG 5 cut(s) 118, 134, 150, 160, 311
FriOI GRGCYC 1 cut(s) 1146
Fsp4HI GCNGC 6 cut(s) 335, 407, 507, 844, 921, 1036
FspBI CTAG 1 cut(s) 248
GlaI GCGC 1 cut(s) 730
GluI GCNGC 6 cut(s) 335, 407, 507, 844, 921, 1036
HaeIII GGCC 6 cut(s) 92, 602, 655, 749, 836, 1035
HapII CCGG 4 cut(s) 341, 403, 1136, 1196
HgaI GACGC 1 cut(s) 710
HhaI GCGC 1 cut(s) 731
Hin1II CATG 6 cut(s) 231, 272, 276, 394, 680, 1282
Hin6I GCGC 1 cut(s) 729
HinP1I GCGC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 1242
HinfI GANTC 4 cut(s) 64, 233, 516, 1199
HpaII CCGG 4 cut(s) 341, 403, 1136, 1196
HphI GGTGA 2 cut(s) 256, 1090
Hpy166II GTNNAC 2 cut(s) 379, 442
Hpy188I TCNGA 7 cut(s) 136, 196, 448, 577, 763, 814, 913
Hpy188III TCNNGA 6 cut(s) 220, 248, 259, 1136, 1196, 1252
Hpy8I GTNNAC 2 cut(s) 379, 442
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 187, 338, 904
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 9 cut(s) 28, 53, 100, 272, 586, 846, 920, 953, 1207
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 464, 629, 883
HpyF3I CTNAG 3 cut(s) 491, 717, 811
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 6 cut(s) 231, 272, 276, 394, 680, 1282
HspAI GCGC 1 cut(s) 729
Kpn2I TCCGGA 2 cut(s) 1135, 1195
KroI GCCGGC 1 cut(s) 402
KroNI GCCGGC 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 261
KspI CCGCGG 1 cut(s) 1039
Kzo9I GATC 3 cut(s) 261, 539, 687
LguI GCTCTTC 1 cut(s) 1134
Lsp1109I GCAGC 5 cut(s) 346, 418, 518, 830, 932
LweI GCATC 4 cut(s) 100, 610, 855, 922
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 3 cut(s) 38, 421, 1267
MalI GATC 3 cut(s) 263, 541, 689
MboI GATC 3 cut(s) 261, 539, 687
MboII GAAGA 8 cut(s) 20, 32, 461, 647, 715, 1020, 1151, 1240
MhlI GDGCHC 1 cut(s) 1146
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 4 cut(s) 101, 667, 859, 1047
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 3 cut(s) 92, 772, 786
Mox20I TGGCCA 1 cut(s) 92
Mph1103I ATGCAT 1 cut(s) 274
MroI TCCGGA 2 cut(s) 1135, 1195
MroNI GCCGGC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 499
MscI TGGCCA 1 cut(s) 92
MseI TTAA 4 cut(s) 956, 981, 1026, 1068
MslI CAYNNNNRTG 3 cut(s) 389, 485, 554
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 1038
MspI CCGG 4 cut(s) 341, 403, 1136, 1196
MspR9I CCNGG 3 cut(s) 73, 599, 768
MvaI CCWGG 3 cut(s) 73, 599, 768
MvnI CGCG 2 cut(s) 300, 1038
MwoI GCNNNNNNNGC 4 cut(s) 89, 464, 629, 883
NaeI GCCGGC 1 cut(s) 404
NdeI CATATG 1 cut(s) 975
NdeII GATC 3 cut(s) 261, 539, 687
NgoMIV GCCGGC 1 cut(s) 402
NlaIII CATG 6 cut(s) 231, 272, 276, 394, 680, 1282
NlaIV GGNNCC 1 cut(s) 750
NmuCI GTSAC 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 276
PaeI GCATGC 1 cut(s) 276
PceI AGGCCT 1 cut(s) 836
PciSI GCTCTTC 1 cut(s) 1134
PdiI GCCGGC 1 cut(s) 404
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 3 cut(s) 64, 516, 1199
PkrI GCNGC 6 cut(s) 336, 408, 508, 845, 922, 1037
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 595
Psp124BI GAGCTC 1 cut(s) 1146
Psp5II RGGWCCY 1 cut(s) 595
Psp6I CCWGG 3 cut(s) 71, 597, 766
PspGI CCWGG 3 cut(s) 71, 597, 766
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 2 cut(s) 595, 748
PspPPI RGGWCCY 1 cut(s) 595
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 2 cut(s) 259, 598
RsaI GTAC 3 cut(s) 129, 479, 1224
RsaNI GTAC 3 cut(s) 128, 478, 1223
RseI CAYNNNNRTG 3 cut(s) 389, 485, 554
SacI GAGCTC 1 cut(s) 1146
SacII CCGCGG 1 cut(s) 1039
SapI GCTCTTC 1 cut(s) 1134
SaqAI TTAA 4 cut(s) 956, 981, 1026, 1068
SatI GCNGC 6 cut(s) 335, 407, 507, 844, 921, 1036
Sau3AI GATC 3 cut(s) 261, 539, 687
Sau96I GGNCC 2 cut(s) 595, 748
ScaI AGTACT 1 cut(s) 1224
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 3 cut(s) 73, 599, 768
SduI GDGCHC 1 cut(s) 1146
SfaNI GCATC 4 cut(s) 100, 610, 855, 922
SfcI CTRYAG 1 cut(s) 26
Sfr303I CCGCGG 1 cut(s) 1039
SgrBI CCGCGG 1 cut(s) 1039
SinI GGWCC 1 cut(s) 595
SmiMI CAYNNNNRTG 3 cut(s) 389, 485, 554
SmlI CTYRAG 3 cut(s) 697, 830, 1128
SmoI CTYRAG 3 cut(s) 697, 830, 1128
SphI GCATGC 1 cut(s) 276
Sse9I AATT 4 cut(s) 101, 667, 859, 1047
SseBI AGGCCT 1 cut(s) 836
SsiI CCGC 6 cut(s) 300, 431, 632, 754, 1036, 1038
SspI AATATT 1 cut(s) 581
SspMI CTAG 1 cut(s) 248
SstI GAGCTC 1 cut(s) 1146
StuI AGGCCT 1 cut(s) 836
StyD4I CCNGG 3 cut(s) 71, 597, 766
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 1 cut(s) 187
TaqI TCGA 1 cut(s) 649
TaqII GACCGA 1 cut(s) 528
TasI AATT 4 cut(s) 101, 667, 859, 1047
TatI WGTACW 2 cut(s) 477, 1222
TauI GCSGC 1 cut(s) 1038
TfiI GAWTC 3 cut(s) 64, 516, 1199
Tru1I TTAA 4 cut(s) 956, 981, 1026, 1068
Tru9I TTAA 4 cut(s) 956, 981, 1026, 1068
TscAI CASTG 3 cut(s) 19, 351, 1008
TseFI GTSAC 1 cut(s) 421
TseI GCWGC 5 cut(s) 334, 406, 506, 843, 920
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 2 cut(s) 152, 326
TspGWI ACGGA 2 cut(s) 95, 203
TspRI CASTG 3 cut(s) 19, 351, 1008
VpaK11BI GGWCC 1 cut(s) 595
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 276
XcmI CCANNNNNNNNNTGG 1 cut(s) 648
XmiI GTMKAC 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 1224
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.