Rh6DG387400

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000446
Physical Location & Seq
Reverse (-)
58023035 .. 58024846
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG387400.1

Sequence Viewer

Length: 924 bp
ATGGCCGAGTTGCTAGACCTGCCAGAAGTCCTGATTATCCAACCACCACCGCCCTTCACATCTTTCGAATCCGACTTCACCCAAAAGTTCAACATACTGCGAGCATGGGAATCATCTCTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCAGGCCCTCCTTTGCCACGTTTTCACTCCCGTCAACGGCGACCTCCTCCGGCTGCTACCGGCGCTGAAGCTCGTGGTGTCTGTCAGCACCGGCGCCAACCACGTGGACAAAGCTGAGTGCCGGAGGCGTGGGATTGCGGTGACCAATGCCGGAAATGCGTACTCGGAAGATGTTGCTGATATGGCTGTTGGGTTGTTCATCGATGTGAATCGGATAATTTCAGCAGCTGATTGGTACGTTAGACGTGGGCTTTGGTCTAGCAAGGGAGACTATCCTCTTGGTTCCAAGATAAGTGGAAAGCGAGTAGGTATTGTTGGATTGGGAAACATTGGCTATGAAGTTGCCAAAAGACTTGAGGGCTTTGGCTGCAAGATCTCATACAACTCAAGGAGCGAAAGGCCGTCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAGTGATGCCAACAAGGAAGTCTTATCTGCGTTGGGAAAAGATGGGGTGGTAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGAGCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGGCTGTTCTTACCCTAGAGTCTTTCAAGAGAGGAAGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAACAAACCTTTGGTTACTCCAGTCATGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

307

Amino Acids

33.46

Weight (kDa)

5.65

Isoelectric Point (pI)

44.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 305 3.2e-20 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 274 7.3e-35 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 251
AciI CCGC 2 cut(s) 50, 296
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 245
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 320, 395
AfiI CCNNNNNNNGG 1 cut(s) 194
AgsI TTSAA 2 cut(s) 91, 844
AjiI CACGTC 1 cut(s) 404
AjnI CCWGG 1 cut(s) 159
AluBI AGCT 7 cut(s) 229, 272, 386, 598, 605, 781, 881
AluI AGCT 7 cut(s) 229, 272, 386, 598, 605, 781, 881
Alw26I GTCTC 2 cut(s) 420, 813
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 3 cut(s) 3, 162, 557
ApeKI GCWGC 4 cut(s) 211, 383, 525, 602
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AspLEI GCGC 2 cut(s) 223, 254
AspS9I GGNCC 2 cut(s) 127, 163
AsuHPI GGTGA 4 cut(s) 70, 130, 310, 727
AsuII TTCGAA 1 cut(s) 66
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BauI CACGAG 1 cut(s) 230
BbrPI CACGTG 1 cut(s) 262
BbvI GCAGC 4 cut(s) 198, 395, 512, 614
BccI CCATC 1 cut(s) 644
BceAI ACGGC 2 cut(s) 211, 544
BciT130I CCWGG 1 cut(s) 161
BcoDI GTCTC 2 cut(s) 420, 813
BfaI CTAG 3 cut(s) 14, 417, 833
BfoI RGCGCY 2 cut(s) 224, 255
BfuAI ACCTGC 1 cut(s) 27
BglII AGATCT 1 cut(s) 531
BisI GCNGC 4 cut(s) 212, 384, 526, 603
BlsI GCNGC 4 cut(s) 213, 385, 527, 604
Bme1390I CCNGG 1 cut(s) 161
Bme18I GGWCC 1 cut(s) 127
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 2 cut(s) 127, 163
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 1 cut(s) 161
BmsI GCATC 1 cut(s) 604
BpmI CTGGAG 1 cut(s) 894
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 529, 533
Bsa29I ATCGAT 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 262
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 2 cut(s) 217, 248
Bse1I ACTGG 2 cut(s) 130, 911
Bse3DI GCAATG 1 cut(s) 795
Bse8I GATNNNNATC 1 cut(s) 366
BseBI CCWGG 1 cut(s) 161
BseCI ATCGAT 1 cut(s) 360
BseGI GGATG 1 cut(s) 751
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 1 cut(s) 264
BseNI ACTGG 2 cut(s) 130, 911
BseRI GAGGAG 1 cut(s) 194
BseXI GCAGC 4 cut(s) 198, 395, 512, 614
BshFI GGCC 3 cut(s) 5, 164, 559
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiSI CCGG 5 cut(s) 208, 218, 249, 280, 309
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 420, 813
BsnI GGCC 3 cut(s) 5, 164, 559
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 3 cut(s) 531, 726, 864
BspACI CCGC 2 cut(s) 50, 296
BspANI GGCC 3 cut(s) 5, 164, 559
BspCNI CTCAG 1 cut(s) 265
BspDI ATCGAT 1 cut(s) 360
BspLI GGNNCC 2 cut(s) 253, 442
BspMI ACCTGC 1 cut(s) 27
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 795
BsrFI RCCGGY 2 cut(s) 217, 248
BsrI ACTGG 2 cut(s) 130, 911
BssAI RCCGGY 2 cut(s) 217, 248
BssMI GATC 3 cut(s) 531, 726, 864
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 230
Bst2BI CACGAG 1 cut(s) 230
Bst2UI CCWGG 1 cut(s) 161
BstACI GRCGYC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 102, 600
BstDEI CTNAG 1 cut(s) 273
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 1 cut(s) 751
BstH2I RGCGCY 2 cut(s) 224, 255
BstHHI GCGC 2 cut(s) 223, 254
BstKTI GATC 3 cut(s) 534, 729, 867
BstMAI GTCTC 2 cut(s) 420, 813
BstMBI GATC 3 cut(s) 531, 726, 864
BstMWI GCNNNNNNNGC 5 cut(s) 19, 220, 314, 341, 525
BstNI CCWGG 1 cut(s) 161
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 159
BstV1I GCAGC 4 cut(s) 198, 395, 512, 614
BstX2I RGATCY 1 cut(s) 531
BstXI CCANNNNNNTGG 1 cut(s) 262
BstYI RGATCY 1 cut(s) 531
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 3 cut(s) 5, 164, 559
BsuTUI ATCGAT 1 cut(s) 360
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 1 cut(s) 751
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 102, 600
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 2 cut(s) 223, 254
Cfr10I RCCGGY 2 cut(s) 217, 248
Cfr13I GGNCC 2 cut(s) 127, 163
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 2 cut(s) 319, 394
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 2 cut(s) 105, 916
CviQI GTAC 2 cut(s) 319, 394
DdeI CTNAG 1 cut(s) 273
DinI GGCGCC 1 cut(s) 253
DpnI GATC 3 cut(s) 533, 728, 866
DpnII GATC 3 cut(s) 531, 726, 864
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 262
Eco91I GGTNACC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 159
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 2 cut(s) 108, 919
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 614, 646
FatI CATG 2 cut(s) 104, 915
Fnu4HI GCNGC 4 cut(s) 212, 384, 526, 603
FokI GGATG 1 cut(s) 758
Fsp4HI GCNGC 4 cut(s) 212, 384, 526, 603
FspBI CTAG 3 cut(s) 14, 417, 833
GlaI GCGC 2 cut(s) 222, 253
GluI GCNGC 4 cut(s) 212, 384, 526, 603
GsuI CTGGAG 1 cut(s) 894
HaeII RGCGCY 2 cut(s) 224, 255
HaeIII GGCC 3 cut(s) 5, 164, 559
HapII CCGG 5 cut(s) 208, 218, 249, 280, 309
HhaI GCGC 2 cut(s) 223, 254
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 2 cut(s) 108, 919
Hin6I GCGC 2 cut(s) 221, 252
HinP1I GCGC 2 cut(s) 221, 252
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HindIII AAGCTT 1 cut(s) 879
HinfI GANTC 4 cut(s) 68, 110, 367, 836
HpaII CCGG 5 cut(s) 208, 218, 249, 280, 309
HphI GGTGA 4 cut(s) 70, 130, 310, 727
Hpy166II GTNNAC 2 cut(s) 193, 265
Hpy188I TCNGA 4 cut(s) 73, 325, 372, 731
Hpy188III TCNNGA 3 cut(s) 31, 125, 844
Hpy8I GTNNAC 2 cut(s) 193, 265
HpyAV CCTTC 2 cut(s) 64, 586
HpyCH4IV ACGT 4 cut(s) 177, 261, 396, 403
HpyCH4V TGCA 2 cut(s) 528, 602
HpyF10VI GCNNNNNNNGC 5 cut(s) 19, 220, 314, 341, 525
HpyF3I CTNAG 1 cut(s) 273
HpySE526I ACGT 4 cut(s) 177, 261, 396, 403
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 2 cut(s) 108, 919
HspAI GCGC 2 cut(s) 221, 252
KasI GGCGCC 1 cut(s) 251
Kzo9I GATC 3 cut(s) 531, 726, 864
LmnI GCTCC 1 cut(s) 549
Lsp1109I GCAGC 4 cut(s) 198, 395, 512, 614
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 14, 417, 833
MaeII ACGT 4 cut(s) 177, 261, 396, 403
MaeIII GTNAC 2 cut(s) 298, 904
MalI GATC 3 cut(s) 533, 728, 866
MboI GATC 3 cut(s) 531, 726, 864
MboII GAAGA 2 cut(s) 338, 877
MflI RGATCY 1 cut(s) 531
MluCI AATT 4 cut(s) 375, 681, 698, 871
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 4 cut(s) 64, 96, 454, 915
MseI TTAA 2 cut(s) 663, 922
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 5 cut(s) 208, 218, 249, 280, 309
MspR9I CCNGG 1 cut(s) 161
MvaI CCWGG 1 cut(s) 161
MwoI GCNNNNNNNGC 5 cut(s) 19, 220, 314, 341, 525
NarI GGCGCC 1 cut(s) 252
NdeII GATC 3 cut(s) 531, 726, 864
NlaIII CATG 2 cut(s) 108, 919
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 66
PfeI GAWTC 3 cut(s) 68, 110, 367
PkrI GCNGC 4 cut(s) 213, 385, 527, 604
PleI GAGTC 1 cut(s) 844
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 262
Psp6I CCWGG 1 cut(s) 159
PspCI CACGTG 1 cut(s) 262
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 159
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 2 cut(s) 127, 163
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 1 cut(s) 531
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 320, 395
RsaNI GTAC 2 cut(s) 319, 394
RseI CAYNNNNRTG 1 cut(s) 362
SaqAI TTAA 2 cut(s) 663, 922
SatI GCNGC 4 cut(s) 212, 384, 526, 603
Sau3AI GATC 3 cut(s) 531, 726, 864
Sau96I GGNCC 2 cut(s) 127, 163
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 1 cut(s) 161
SfaNI GCATC 1 cut(s) 604
SfoI GGCGCC 1 cut(s) 253
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 4 cut(s) 375, 681, 698, 871
SsiI CCGC 2 cut(s) 50, 296
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 3 cut(s) 14, 417, 833
StyD4I CCNGG 1 cut(s) 159
TaiI ACGT 4 cut(s) 180, 264, 399, 406
TaqI TCGA 2 cut(s) 66, 360
TasI AATT 4 cut(s) 375, 681, 698, 871
TfiI GAWTC 3 cut(s) 68, 110, 367
Tru1I TTAA 2 cut(s) 663, 922
Tru9I TTAA 2 cut(s) 663, 922
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 4 cut(s) 211, 383, 525, 602
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 346, 510
VpaK11BI GGWCC 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 898
XspI CTAG 3 cut(s) 14, 417, 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.