Rh1DG230800

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000446
Physical Location & Seq
Forward (+)
44371026 .. 44399732
28707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG230800.1

Sequence Viewer

Length: 606 bp
ATGAGAAAGATTTCGGCTTCGAATCGGTATGTTAGGGATGGGCTCTGGGCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTAGAAGTTGCAAAACGATTGGAGGCATTTGGCTGCACCGTCTTGTACAATTCGAGGAGTGAAAAGCCAGCTTTGTCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATAATCTGCTGTGCATTGACAGCGGAAACCCACCACATGATCAACAAGGAAGTATTGTCTGCGTTGGGAAGAGAGGGCGTGATTGTTAATGTAGGGCGCAGAGTTATTATCGATGAGAAGGAAATGGTGCAGTGTTTGGTGCGAGGAGAGATAAGGGGTGCTGGCTTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTTTGCATTGGATAATGTTGTACTGTCACTGCATCAAGCTGTCGCTACCCATGAAGCTATCGTGGCTTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCCGGCTGTGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

201

Amino Acids

21.75

Weight (kDa)

5.84

Isoelectric Point (pI)

25.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh_C PF02826 1 - 167 1.6e-46 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 278
AcsI RAATTY 1 cut(s) 550
AfaI GTAC 2 cut(s) 173, 483
AluBI AGCT 5 cut(s) 197, 232, 460, 500, 518
AluI AGCT 5 cut(s) 197, 232, 460, 500, 518
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 1 cut(s) 54
ApeKI GCWGC 2 cut(s) 159, 236
ApoI RAATTY 1 cut(s) 550
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 1 cut(s) 10
AspLEI GCGC 1 cut(s) 354
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 1 cut(s) 20
BanII GRGCYC 2 cut(s) 45, 462
Bbv12I GWGCWC 1 cut(s) 462
BbvI GCAGC 2 cut(s) 146, 248
BccI CCATC 1 cut(s) 32
BclI TGATCA 1 cut(s) 294
BcoDI GTCTC 1 cut(s) 54
BisI GCNGC 2 cut(s) 160, 237
BlsI GCNGC 2 cut(s) 161, 238
BmsI GCATC 2 cut(s) 238, 502
Bpu14I TTCGAA 1 cut(s) 20
Bsa29I ATCGAT 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 366
BseGI GGATG 2 cut(s) 43, 430
BseRI GAGGAG 3 cut(s) 72, 196, 414
BseXI GCAGC 2 cut(s) 146, 248
BsgI GTGCAG 2 cut(s) 145, 404
BshVI ATCGAT 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 462
BsiSI CCGG 1 cut(s) 590
BsmAI GTCTC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 560
Bsp119I TTCGAA 1 cut(s) 20
Bsp1286I GDGCHC 2 cut(s) 45, 462
Bsp1407I TGTACA 1 cut(s) 171
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 278
BspDI ATCGAT 1 cut(s) 366
BspT104I TTCGAA 1 cut(s) 20
BsrGI TGTACA 1 cut(s) 171
BssMI GATC 1 cut(s) 294
Bst4CI ACNGT 4 cut(s) 166, 245, 486, 579
Bst6I CTCTTC 1 cut(s) 319
BstAUI TGTACA 1 cut(s) 171
BstBI TTCGAA 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 195, 234, 418
BstDEI CTNAG 1 cut(s) 78
BstF5I GGATG 2 cut(s) 43, 430
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 2 cut(s) 275, 524
BstV1I GCAGC 2 cut(s) 146, 248
Bsu15I ATCGAT 1 cut(s) 366
BsuTUI ATCGAT 1 cut(s) 366
BtsCI GGATG 2 cut(s) 43, 430
BtsI GCAGTG 2 cut(s) 392, 488
BtsIMutI CAGTG 3 cut(s) 250, 392, 488
Cac8I GCNNGC 3 cut(s) 195, 234, 418
CfoI GCGC 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 366
Csp6I GTAC 2 cut(s) 172, 482
CviAII CATG 2 cut(s) 292, 512
CviQI GTAC 2 cut(s) 172, 482
DdeI CTNAG 1 cut(s) 78
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 319
Ecl136II GAGCTC 1 cut(s) 460
Eco24I GRGCYC 2 cut(s) 45, 462
Eco53kI GAGCTC 1 cut(s) 460
EcoICRI GAGCTC 1 cut(s) 460
EcoT38I GRGCYC 2 cut(s) 45, 462
FaeI CATG 2 cut(s) 295, 515
FaiI YATR 5 cut(s) 30, 205, 257, 293, 513
FatI CATG 2 cut(s) 291, 511
FbaI TGATCA 1 cut(s) 294
Fnu4HI GCNGC 2 cut(s) 160, 237
FokI GGATG 2 cut(s) 50, 437
FriOI GRGCYC 2 cut(s) 45, 462
Fsp4HI GCNGC 2 cut(s) 160, 237
GlaI GCGC 1 cut(s) 353
GluI GCNGC 2 cut(s) 160, 237
HapII CCGG 1 cut(s) 590
HhaI GCGC 1 cut(s) 354
Hin1II CATG 2 cut(s) 295, 515
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HinfI GANTC 1 cut(s) 22
HpaII CCGG 1 cut(s) 590
HphI GGTGA 1 cut(s) 545
HpyAV CCTTC 2 cut(s) 88, 367
HpyCH4III ACNGT 4 cut(s) 166, 245, 486, 579
HpyCH4V TGCA 7 cut(s) 137, 162, 236, 269, 385, 467, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 524
HpyF3I CTNAG 1 cut(s) 78
Hsp92II CATG 2 cut(s) 295, 515
HspAI GCGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 294
Kzo9I GATC 1 cut(s) 294
LpnPI CCDG 6 cut(s) 31, 67, 207, 402, 456, 531
Lsp1109I GCAGC 2 cut(s) 146, 248
LweI GCATC 2 cut(s) 238, 502
MaeIII GTNAC 2 cut(s) 486, 541
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 2 cut(s) 336, 556
MhlI GDGCHC 2 cut(s) 45, 462
MluCI AATT 5 cut(s) 175, 209, 536, 550, 601
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 7 cut(s) 50, 78, 80, 142, 174, 322, 392
MroXI GAANNNNTTC 1 cut(s) 10
MseI TTAA 2 cut(s) 342, 600
MspA1I CMGCKG 1 cut(s) 278
MspI CCGG 1 cut(s) 590
Mva1269I GAATGC 1 cut(s) 560
MwoI GCNNNNNNNGC 2 cut(s) 275, 524
NdeII GATC 1 cut(s) 294
NlaIII CATG 2 cut(s) 295, 515
NmuCI GTSAC 1 cut(s) 486
NspV TTCGAA 1 cut(s) 20
PctI GAATGC 1 cut(s) 560
PdmI GAANNNNTTC 1 cut(s) 10
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 2 cut(s) 161, 238
PshBI ATTAAT 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 462
RsaI GTAC 2 cut(s) 173, 483
RsaNI GTAC 2 cut(s) 172, 482
SacI GAGCTC 1 cut(s) 462
SaqAI TTAA 2 cut(s) 342, 600
SatI GCNGC 2 cut(s) 160, 237
Sau3AI GATC 1 cut(s) 294
SduI GDGCHC 2 cut(s) 45, 462
SetI ASST 6 cut(s) 99, 199, 234, 462, 502, 520
SfaNI GCATC 2 cut(s) 238, 502
SfuI TTCGAA 1 cut(s) 20
Sse9I AATT 5 cut(s) 175, 209, 536, 550, 601
SsiI CCGC 1 cut(s) 278
SstI GAGCTC 1 cut(s) 462
TaaI ACNGT 4 cut(s) 166, 245, 486, 579
TaqI TCGA 3 cut(s) 20, 179, 366
TasI AATT 5 cut(s) 175, 209, 536, 550, 601
TatI WGTACW 2 cut(s) 171, 481
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 2 cut(s) 342, 600
Tru9I TTAA 2 cut(s) 342, 600
TscAI CASTG 3 cut(s) 250, 392, 495
TseFI GTSAC 1 cut(s) 486
TseI GCWGC 2 cut(s) 159, 236
Tsp45I GTSAC 1 cut(s) 486
TspDTI ATGAA 1 cut(s) 528
TspRI CASTG 3 cut(s) 250, 392, 495
VspI ATTAAT 1 cut(s) 600
XapI RAATTY 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.