MD07G1124500.v1.1

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000446
Physical Location & Seq
Reverse (-)
17406963 .. 17408957
1995 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1124500.v1.1.491

Sequence Viewer

Length: 981 bp
ATGGCACAAGACAATCCCACCCAACTCCCTCAAGTACTGCTTCTTCTCCCAAATCCCTGCTTCACAGTCCTGGAGTCCAAAGCCTCTCACAAGTTCCACTTCCTCAAAGCCTGGGACTCCCCTCTCCCTCAGGCCCAGTTCCTCCAGTCCCACGCTCTCTCCACCCAGGTCCTCCTCTCCTCCGCCCTCGGACCCCCCATCACCGCCCAGCTCCTCCAATTACTTCCCTCCTTGAAGCTCCTAATCACCCTCAGCGCAGGCCTTGACCATGTTGACTTTTCCGCGTGTGCAGCACGCGGCGTGCGCATTGCCAATTCCTCTCCAATCTTCGCTGCCGATGTCGCTGACATGGCTGTGGGGTTGTTTTTGGATGTCATGAGGAAGATTTCGGCGGCTAATCGGTTTGTCCGAGATGGGTTTTGGGTTAGCAAAGGAGATTTTGATCTCGGTACTAAGATAGGAGGGAAGCGAGTTGGTATCGTTGGATTGGGACATATTGGCTTACAAGTTGCTAAAAGACTTGAGGCATTTGACTGCAAAATCTTGTACTACTCAAGGAAGGAAAAACCATATGTTTCATACCCTTTCTATTTCGATGTTTGTGAACTTGCAGCTAATAGCGACGTGCTTGTCATTTGCTGTGCGTTGACTGCTCAAACGCACCACATGATTAACAAGAAAATCTTGTTGGCGTTGGGAAGAGAGGGAGTTATTGTTAATGTAGGGCGTGGAGCTATTATCGATGAGAAGGAAATGGTACAGTGTTTGGTACAAGGAGAGATCAAAGGTGCAGGCTTGGATGTGTTTGAGAATGAGCCTCAAGTTCCGAAAGAGCTCTTTGGACTAGGTAATGTTGTTTTGTCGCCACATAAAGCTGCCATGACAGCAGACTGTTTCACGGATTTGACTGATCTAGCAATGGCGAATTTGGAGGCATTCTTCTCAAATAAACCATTGCTTTCTGAAGTGACGAATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

327

Amino Acids

35.41

Weight (kDa)

6.24

Isoelectric Point (pI)

31.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 63 - 323 7e-15 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 6.8e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 629
Acc16I TGCGCA 1 cut(s) 305
AccII CGCG 2 cut(s) 284, 297
AciI CCGC 5 cut(s) 183, 204, 282, 297, 392
AcsI RAATTY 1 cut(s) 925
AfaI GTAC 5 cut(s) 36, 451, 548, 759, 771
AgsI TTSAA 1 cut(s) 235
AjiI CACGTC 1 cut(s) 625
AjnI CCWGG 3 cut(s) 69, 110, 165
AjuI GAANNNNNNNTTGG 2 cut(s) 671, 703
AluBI AGCT 6 cut(s) 211, 238, 614, 734, 835, 875
AluI AGCT 6 cut(s) 211, 238, 614, 734, 835, 875
Alw21I GWGCWC 1 cut(s) 837
AoxI GGCC 2 cut(s) 132, 259
ApeKI GCWGC 4 cut(s) 290, 332, 611, 875
ApoI RAATTY 1 cut(s) 925
AspLEI GCGC 2 cut(s) 257, 306
AspS9I GGNCC 3 cut(s) 133, 169, 191
AsuHPI GGTGA 2 cut(s) 193, 238
AvaII GGWCC 2 cut(s) 169, 191
AxyI CCTNAGG 1 cut(s) 129
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 27, 59
Bbv12I GWGCWC 1 cut(s) 837
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 4 cut(s) 302, 319, 623, 862
BccI CCATC 2 cut(s) 206, 407
BciT130I CCWGG 3 cut(s) 71, 112, 167
BfaI CTAG 2 cut(s) 845, 914
BisI GCNGC 6 cut(s) 291, 298, 333, 393, 612, 876
BlsI GCNGC 6 cut(s) 292, 299, 334, 394, 613, 877
BmcAI AGTACT 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 71, 112, 167
Bme18I GGWCC 2 cut(s) 169, 191
BmgBI CACGTC 1 cut(s) 625
BmgT120I GGNCC 3 cut(s) 133, 169, 191
BmiI GGNNCC 1 cut(s) 193
BmrFI CCNGG 3 cut(s) 71, 112, 167
BmrI ACTGGG 1 cut(s) 130
BmuI ACTGGG 1 cut(s) 130
BpmI CTGGAG 2 cut(s) 92, 128
Bpu10I CCTNAGC 1 cut(s) 251
BpuEI CTTGAG 4 cut(s) 15, 538, 542, 804
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 111, 165, 187
BsaXI ACNNNNNCTCC 4 cut(s) 143, 173, 699, 729
Bse1I ACTGG 2 cut(s) 136, 145
Bse21I CCTNAGG 1 cut(s) 129
Bse3DI GCAATG 3 cut(s) 306, 924, 953
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 3 cut(s) 71, 112, 167
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 3 cut(s) 111, 165, 187
BseGI GGATG 2 cut(s) 376, 805
BseJI GATNNNNATC 1 cut(s) 441
BseMI GCAATG 3 cut(s) 306, 924, 953
BseMII CTCAG 2 cut(s) 143, 265
BseNI ACTGG 2 cut(s) 136, 145
BseRI GAGGAG 3 cut(s) 164, 169, 203
BseXI GCAGC 4 cut(s) 302, 319, 623, 862
BseYI CCCAGC 1 cut(s) 207
BsgI GTGCAG 2 cut(s) 309, 810
Bsh1236I CGCG 2 cut(s) 284, 297
BshFI GGCC 2 cut(s) 134, 261
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 837
BslFI GGGAC 3 cut(s) 128, 133, 504
BsmFI GGGAC 3 cut(s) 128, 133, 504
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 2 cut(s) 134, 261
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 3 cut(s) 442, 780, 910
BspACI CCGC 5 cut(s) 183, 204, 282, 297, 392
BspANI GGCC 2 cut(s) 134, 261
BspCNI CTCAG 2 cut(s) 142, 264
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 2 cut(s) 284, 297
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 193
BsrDI GCAATG 3 cut(s) 306, 924, 953
BsrI ACTGG 2 cut(s) 136, 145
BssECI CCNNGG 3 cut(s) 111, 165, 187
BssMI GATC 3 cut(s) 442, 780, 910
Bst2UI CCWGG 3 cut(s) 71, 112, 167
Bst4CI ACNGT 3 cut(s) 67, 762, 893
Bst6I CTCTTC 1 cut(s) 694
BstC8I GCNNGC 4 cut(s) 259, 295, 302, 793
BstDEI CTNAG 3 cut(s) 129, 251, 453
BstF5I GGATG 2 cut(s) 376, 805
BstFNI CGCG 2 cut(s) 284, 297
BstHHI GCGC 2 cut(s) 257, 306
BstKTI GATC 3 cut(s) 445, 783, 913
BstMBI GATC 3 cut(s) 442, 780, 910
BstMWI GCNNNNNNNGC 6 cut(s) 290, 303, 341, 350, 650, 884
BstNI CCWGG 3 cut(s) 71, 112, 167
BstSCI CCNGG 3 cut(s) 69, 110, 165
BstUI CGCG 2 cut(s) 284, 297
BstV1I GCAGC 4 cut(s) 302, 319, 623, 862
Bsu15I ATCGAT 1 cut(s) 741
Bsu36I CCTNAGG 1 cut(s) 129
BsuRI GGCC 2 cut(s) 134, 261
BsuTUI ATCGAT 1 cut(s) 741
BtrI CACGTC 1 cut(s) 625
BtsCI GGATG 2 cut(s) 376, 805
BtsIMutI CAGTG 1 cut(s) 767
Cac8I GCNNGC 4 cut(s) 259, 295, 302, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 257, 306
Cfr13I GGNCC 3 cut(s) 133, 169, 191
ClaI ATCGAT 1 cut(s) 741
Csp6I GTAC 5 cut(s) 35, 450, 547, 758, 770
CviAII CATG 5 cut(s) 269, 349, 376, 667, 880
CviQI GTAC 5 cut(s) 35, 450, 547, 758, 770
DdeI CTNAG 3 cut(s) 129, 251, 453
DpnI GATC 3 cut(s) 444, 782, 912
DpnII GATC 3 cut(s) 442, 780, 910
DrdI GACNNNNNNGTC 1 cut(s) 629
DseDI GACNNNNNNGTC 1 cut(s) 629
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 835
Eco147I AGGCCT 1 cut(s) 261
Eco24I GRGCYC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 169, 191
Eco53kI GAGCTC 1 cut(s) 835
Eco81I CCTNAGG 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 69, 110, 165
EcoT38I GRGCYC 1 cut(s) 837
FaeI CATG 5 cut(s) 272, 352, 379, 670, 883
FalI AAGNNNNNCTT 6 cut(s) 24, 56, 83, 115, 668, 700
FaqI GGGAC 3 cut(s) 128, 133, 504
FatI CATG 5 cut(s) 268, 348, 375, 666, 879
FauNDI CATATG 1 cut(s) 571
Fnu4HI GCNGC 6 cut(s) 291, 298, 333, 393, 612, 876
FokI GGATG 2 cut(s) 383, 812
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 6 cut(s) 291, 298, 333, 393, 612, 876
FspAI RTGCGCAY 1 cut(s) 305
FspBI CTAG 2 cut(s) 845, 914
FspI TGCGCA 1 cut(s) 305
GlaI GCGC 2 cut(s) 256, 305
GluI GCNGC 6 cut(s) 291, 298, 333, 393, 612, 876
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 2 cut(s) 92, 128
HaeIII GGCC 2 cut(s) 134, 261
HhaI GCGC 2 cut(s) 257, 306
Hin1II CATG 5 cut(s) 272, 352, 379, 670, 883
Hin6I GCGC 2 cut(s) 255, 304
HinP1I GCGC 2 cut(s) 255, 304
HincII GTYRAC 2 cut(s) 274, 648
HindII GTYRAC 2 cut(s) 274, 648
HinfI GANTC 2 cut(s) 74, 116
HphI GGTGA 2 cut(s) 193, 238
Hpy166II GTNNAC 3 cut(s) 274, 605, 648
Hpy188I TCNGA 4 cut(s) 191, 410, 828, 964
Hpy188III TCNNGA 1 cut(s) 376
Hpy8I GTNNAC 3 cut(s) 274, 605, 648
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 2 cut(s) 553, 742
HpyCH4III ACNGT 3 cut(s) 67, 762, 893
HpyCH4IV ACGT 1 cut(s) 624
HpyCH4V TGCA 4 cut(s) 290, 537, 611, 791
HpyF10VI GCNNNNNNNGC 6 cut(s) 290, 303, 341, 350, 650, 884
HpyF3I CTNAG 3 cut(s) 129, 251, 453
HpySE526I ACGT 1 cut(s) 624
Hsp92II CATG 5 cut(s) 272, 352, 379, 670, 883
HspAI GCGC 2 cut(s) 255, 304
Kzo9I GATC 3 cut(s) 442, 780, 910
LmnI GCTCC 3 cut(s) 216, 243, 731
Lsp1109I GCAGC 4 cut(s) 302, 319, 623, 862
MaeI CTAG 2 cut(s) 845, 914
MaeII ACGT 1 cut(s) 624
MaeIII GTNAC 1 cut(s) 967
MalI GATC 3 cut(s) 444, 782, 912
MboI GATC 3 cut(s) 442, 780, 910
MboII GAAGA 5 cut(s) 35, 319, 394, 711, 931
MhlI GDGCHC 1 cut(s) 837
MluCI AATT 3 cut(s) 218, 313, 925
MlyI GAGTC 2 cut(s) 83, 110
MmeI TCCRAC 1 cut(s) 463
MseI TTAA 2 cut(s) 672, 717
MslI CAYNNNNRTG 1 cut(s) 353
MspR9I CCNGG 3 cut(s) 71, 112, 167
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 3 cut(s) 71, 112, 167
MvnI CGCG 2 cut(s) 284, 297
MwoI GCNNNNNNNGC 6 cut(s) 290, 303, 341, 350, 650, 884
NdeI CATATG 1 cut(s) 571
NdeII GATC 3 cut(s) 442, 780, 910
NlaIII CATG 5 cut(s) 272, 352, 379, 670, 883
NlaIV GGNNCC 1 cut(s) 193
NmuCI GTSAC 1 cut(s) 967
NsbI TGCGCA 1 cut(s) 305
PagI TCATGA 1 cut(s) 375
PceI AGGCCT 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 406
PctI GAATGC 1 cut(s) 935
PfoI TCCNGGA 1 cut(s) 69
PkrI GCNGC 6 cut(s) 292, 299, 334, 394, 613, 877
PleI GAGTC 2 cut(s) 82, 110
PpsI GAGTC 2 cut(s) 82, 110
PpuMI RGGWCCY 1 cut(s) 169
Psp124BI GAGCTC 1 cut(s) 837
Psp5II RGGWCCY 1 cut(s) 169
Psp6I CCWGG 3 cut(s) 69, 110, 165
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 3 cut(s) 69, 110, 165
PspN4I GGNNCC 1 cut(s) 193
PspPI GGNCC 3 cut(s) 133, 169, 191
PspPPI RGGWCCY 1 cut(s) 169
RsaI GTAC 5 cut(s) 36, 451, 548, 759, 771
RsaNI GTAC 5 cut(s) 35, 450, 547, 758, 770
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 1 cut(s) 837
SaqAI TTAA 2 cut(s) 672, 717
SatI GCNGC 6 cut(s) 291, 298, 333, 393, 612, 876
Sau3AI GATC 3 cut(s) 442, 780, 910
Sau96I GGNCC 3 cut(s) 133, 169, 191
ScaI AGTACT 1 cut(s) 36
SchI GAGTC 2 cut(s) 83, 110
ScrFI CCNGG 3 cut(s) 71, 112, 167
SduI GDGCHC 1 cut(s) 837
SinI GGWCC 2 cut(s) 169, 191
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 4 cut(s) 30, 521, 553, 819
SmoI CTYRAG 4 cut(s) 30, 521, 553, 819
Sse9I AATT 3 cut(s) 218, 313, 925
SseBI AGGCCT 1 cut(s) 261
SsiI CCGC 5 cut(s) 183, 204, 282, 297, 392
SspMI CTAG 2 cut(s) 845, 914
SstI GAGCTC 1 cut(s) 837
StuI AGGCCT 1 cut(s) 261
StyD4I CCNGG 3 cut(s) 69, 110, 165
TaaI ACNGT 3 cut(s) 67, 762, 893
TaiI ACGT 1 cut(s) 627
TaqI TCGA 2 cut(s) 594, 741
TasI AATT 3 cut(s) 218, 313, 925
TatI WGTACW 2 cut(s) 34, 546
TauI GCSGC 2 cut(s) 300, 395
Tru1I TTAA 2 cut(s) 672, 717
Tru9I TTAA 2 cut(s) 672, 717
TscAI CASTG 1 cut(s) 767
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 4 cut(s) 290, 332, 611, 875
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 1 cut(s) 567
TspGWI ACGGA 1 cut(s) 914
TspRI CASTG 1 cut(s) 767
VpaK11BI GGWCC 2 cut(s) 169, 191
XapI RAATTY 1 cut(s) 925
XspI CTAG 2 cut(s) 845, 914
ZrmI AGTACT 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.