Rh1BG203900

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000446
Physical Location & Seq
Forward (+)
32207035 .. 32235631
28597 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG203900.1

Sequence Viewer

Length: 882 bp
ATGGCAGATGCCTGGGACTCTCCTCTCCCTCAAGATCAGTTCTTGACCACCCACGCCAGCTCCGTCCAGGCCCTTATCTCCTCCGCCGATGGGCCACCCATCACCGCCCAAATCCTCCACTTGCTCCCCTCCCTCAAAGTCATAGCCACCACCAGCGCCGTCCTTGACCACGTTGACTTGGCCGAGTGTCGACGCCGCGGCGTTGCCGTCGTCGACGCCGGTAAAGCCTTTGCTGAGGACGCTGCTGACTCGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCGGTATGTTAGGGATGGGCTCTGGGCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTAGAAGTTGCAAAAAGATTGGAGGCATTTGGCTGCAGTGTGTCGTACAATTCGAGGAGCGAAAGGCCGTATTTGTCATACCAATTCTATTCCAATGTGTGTGAGCTTGCATCAGACAGTGATGCTCTCATCATCTGTTGTGCATTGACAGAGCAAACCCACCACATGATCAACAAGGAAGTTTTGTCTGCGTTGGGAAGAGAGGGAGTGGTTGTTAATGTAGGGCGCGGAGCCATTATCGATGAGAAAGAAATGGTGCAGTGTTTGGTGCGAGGAGAGATAAGGGGTGCTGGCCTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTTGGCATTGGATAATGTTGTACTGTCACCGCATCAAGCTGTTCTTACACATGAAGCTTTCGTGGGTTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCCGGCTGTGATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

293

Amino Acids

31.34

Weight (kDa)

5.04

Isoelectric Point (pI)

31.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 17 - 288 6.9e-17 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 87 - 259 1.3e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 190, 213
AccII CGCG 2 cut(s) 198, 630
AciI CCGC 6 cut(s) 84, 105, 196, 198, 630, 767
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 826
AcyI GRCGYC 2 cut(s) 193, 216
AfaI GTAC 2 cut(s) 449, 759
AfiI CCNNNNNNNGG 1 cut(s) 90
AjnI CCWGG 3 cut(s) 11, 66, 696
AluBI AGCT 5 cut(s) 60, 508, 736, 776, 794
AluI AGCT 5 cut(s) 60, 508, 736, 776, 794
Alw21I GWGCWC 1 cut(s) 738
Alw26I GTCTC 1 cut(s) 330
AoxI GGCC 5 cut(s) 69, 92, 180, 467, 694
ApeKI GCWGC 2 cut(s) 242, 435
ApoI RAATTY 1 cut(s) 826
Asp700I GAANNNNTTC 1 cut(s) 286
AspLEI GCGC 2 cut(s) 158, 630
AspS9I GGNCC 2 cut(s) 70, 92
AsuHPI GGTGA 3 cut(s) 94, 756, 821
AsuII TTCGAA 1 cut(s) 296
BanII GRGCYC 2 cut(s) 321, 738
Bbv12I GWGCWC 1 cut(s) 738
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 2 cut(s) 229, 422
BccI CCATC 3 cut(s) 83, 107, 308
BceAI ACGGC 3 cut(s) 143, 191, 454
BciT130I CCWGG 3 cut(s) 13, 68, 698
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 330
BfmI CTRYAG 1 cut(s) 436
BfoI RGCGCY 1 cut(s) 159
BisI GCNGC 4 cut(s) 196, 199, 243, 436
BlsI GCNGC 4 cut(s) 197, 200, 244, 437
Bme1390I CCNGG 3 cut(s) 13, 68, 698
BmgT120I GGNCC 2 cut(s) 70, 92
BmiI GGNNCC 1 cut(s) 634
BmrFI CCNGG 3 cut(s) 13, 68, 698
BmsI GCATC 3 cut(s) 514, 521, 778
Bpu10I CCTNAGC 1 cut(s) 234
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 1 cut(s) 15
Bsa29I ATCGAT 1 cut(s) 642
BsaHI GRCGYC 2 cut(s) 193, 216
BsaJI CCNNGG 2 cut(s) 12, 196
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 600, 630
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse118I RCCGGY 1 cut(s) 218
BseBI CCWGG 3 cut(s) 13, 68, 698
BseCI ATCGAT 1 cut(s) 642
BseDI CCNNGG 2 cut(s) 12, 196
BseGI GGATG 2 cut(s) 319, 706
BseLI CCNNNNNNNGG 1 cut(s) 90
BseMII CTCAG 1 cut(s) 225
BseRI GAGGAG 5 cut(s) 12, 70, 348, 472, 690
BseXI GCAGC 2 cut(s) 229, 422
BsgI GTGCAG 1 cut(s) 680
Bsh1236I CGCG 2 cut(s) 198, 630
BshFI GGCC 5 cut(s) 71, 94, 182, 469, 696
BshVI ATCGAT 1 cut(s) 642
BsiHKAI GWGCWC 1 cut(s) 738
BsiSI CCGG 2 cut(s) 219, 866
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 29
BsmI GAATGC 1 cut(s) 836
BsnI GGCC 5 cut(s) 71, 94, 182, 469, 696
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 2 cut(s) 321, 738
Bsp143I GATC 2 cut(s) 34, 570
BspACI CCGC 6 cut(s) 84, 105, 196, 198, 630, 767
BspANI GGCC 5 cut(s) 71, 94, 182, 469, 696
BspCNI CTCAG 1 cut(s) 226
BspDI ATCGAT 1 cut(s) 642
BspFNI CGCG 2 cut(s) 198, 630
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 634
BspMAI CTGCAG 1 cut(s) 440
BspT104I TTCGAA 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 12, 196
BssMI GATC 2 cut(s) 34, 570
BssNI GRCGYC 2 cut(s) 193, 216
Bst2UI CCWGG 3 cut(s) 13, 68, 698
Bst4CI ACNGT 3 cut(s) 521, 762, 855
Bst6I CTCTTC 1 cut(s) 595
BstACI GRCGYC 2 cut(s) 193, 216
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 3 cut(s) 58, 510, 694
BstDEI CTNAG 2 cut(s) 234, 354
BstDSI CCRYGG 1 cut(s) 196
BstF5I GGATG 2 cut(s) 319, 706
BstFNI CGCG 2 cut(s) 198, 630
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 2 cut(s) 158, 630
BstKTI GATC 2 cut(s) 37, 573
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 2 cut(s) 34, 570
BstMWI GCNNNNNNNGC 3 cut(s) 224, 239, 251
BstNI CCWGG 3 cut(s) 13, 68, 698
BstSCI CCNGG 3 cut(s) 11, 66, 696
BstSFI CTRYAG 1 cut(s) 436
BstUI CGCG 2 cut(s) 198, 630
BstV1I GCAGC 2 cut(s) 229, 422
Bsu15I ATCGAT 1 cut(s) 642
BsuRI GGCC 5 cut(s) 71, 94, 182, 469, 696
BsuTUI ATCGAT 1 cut(s) 642
BtgI CCRYGG 1 cut(s) 196
BtsCI GGATG 2 cut(s) 319, 706
BtsI GCAGTG 2 cut(s) 445, 668
BtsIMutI CAGTG 3 cut(s) 445, 526, 668
Cac8I GCNNGC 3 cut(s) 58, 510, 694
CciI TCATGA 1 cut(s) 276
CfoI GCGC 2 cut(s) 158, 630
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 2 cut(s) 70, 92
Cfr42I CCGCGG 1 cut(s) 199
ClaI ATCGAT 1 cut(s) 642
CseI GACGC 3 cut(s) 201, 224, 248
Csp6I GTAC 2 cut(s) 448, 758
CviAII CATG 3 cut(s) 277, 568, 788
CviQI GTAC 2 cut(s) 448, 758
DdeI CTNAG 2 cut(s) 234, 354
DpnI GATC 2 cut(s) 36, 572
DpnII GATC 2 cut(s) 34, 570
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 595
EciI GGCGGA 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 736
Eco24I GRGCYC 2 cut(s) 321, 738
Eco53kI GAGCTC 1 cut(s) 736
EcoICRI GAGCTC 1 cut(s) 736
EcoO109I RGGNCCY 1 cut(s) 70
EcoRII CCWGG 3 cut(s) 11, 66, 696
EcoT38I GRGCYC 2 cut(s) 321, 738
FaeI CATG 3 cut(s) 280, 571, 791
FaiI YATR 6 cut(s) 143, 278, 306, 481, 569, 789
FalI AAGNNNNNCTT 2 cut(s) 765, 797
FaqI GGGAC 1 cut(s) 29
FatI CATG 3 cut(s) 276, 567, 787
FbaI TGATCA 1 cut(s) 570
FblI GTMKAC 2 cut(s) 190, 213
Fnu4HI GCNGC 4 cut(s) 196, 199, 243, 436
FokI GGATG 2 cut(s) 326, 713
FriOI GRGCYC 2 cut(s) 321, 738
Fsp4HI GCNGC 4 cut(s) 196, 199, 243, 436
GlaI GCGC 2 cut(s) 157, 629
GluI GCNGC 4 cut(s) 196, 199, 243, 436
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 5 cut(s) 71, 94, 182, 469, 696
HapII CCGG 2 cut(s) 219, 866
HgaI GACGC 3 cut(s) 201, 224, 248
HhaI GCGC 2 cut(s) 158, 630
Hin1I GRCGYC 2 cut(s) 193, 216
Hin1II CATG 3 cut(s) 280, 571, 791
Hin6I GCGC 2 cut(s) 156, 628
HinP1I GCGC 2 cut(s) 156, 628
HincII GTYRAC 3 cut(s) 175, 191, 214
HindII GTYRAC 3 cut(s) 175, 191, 214
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 3 cut(s) 17, 248, 298
HpaII CCGG 2 cut(s) 219, 866
HphI GGTGA 3 cut(s) 94, 756, 821
Hpy166II GTNNAC 3 cut(s) 175, 191, 214
Hpy188I TCNGA 1 cut(s) 517
Hpy188III TCNNGA 3 cut(s) 32, 43, 277
Hpy8I GTNNAC 3 cut(s) 175, 191, 214
Hpy99I CGWCG 4 cut(s) 195, 212, 215, 218
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 3 cut(s) 521, 762, 855
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 413, 438, 512, 545, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 224, 239, 251
HpyF3I CTNAG 2 cut(s) 234, 354
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 2 cut(s) 193, 216
Hsp92II CATG 3 cut(s) 280, 571, 791
HspAI GCGC 2 cut(s) 156, 628
Ksp22I TGATCA 1 cut(s) 570
KspI CCGCGG 1 cut(s) 199
Kzo9I GATC 2 cut(s) 34, 570
LmnI GCTCC 4 cut(s) 65, 129, 459, 632
Lsp1109I GCAGC 2 cut(s) 229, 422
LweI GCATC 3 cut(s) 514, 521, 778
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 2 cut(s) 762, 817
MalI GATC 2 cut(s) 36, 572
MboI GATC 2 cut(s) 34, 570
MboII GAAGA 2 cut(s) 612, 832
MhlI GDGCHC 2 cut(s) 321, 738
MluCI AATT 4 cut(s) 451, 485, 812, 826
MlyI GAGTC 2 cut(s) 11, 242
MmeI TCCRAC 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 286
MseI TTAA 1 cut(s) 618
MspA1I CMGCKG 1 cut(s) 198
MspI CCGG 2 cut(s) 219, 866
MspR9I CCNGG 3 cut(s) 13, 68, 698
Mva1269I GAATGC 1 cut(s) 836
MvaI CCWGG 3 cut(s) 13, 68, 698
MvnI CGCG 2 cut(s) 198, 630
MwoI GCNNNNNNNGC 3 cut(s) 224, 239, 251
NdeII GATC 2 cut(s) 34, 570
NlaIII CATG 3 cut(s) 280, 571, 791
NlaIV GGNNCC 1 cut(s) 634
NmeAIII GCCGAG 2 cut(s) 208, 230
NmuCI GTSAC 1 cut(s) 762
NspV TTCGAA 1 cut(s) 296
PagI TCATGA 1 cut(s) 276
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 452
PctI GAATGC 1 cut(s) 836
PdmI GAANNNNTTC 1 cut(s) 286
PfeI GAWTC 1 cut(s) 298
PkrI GCNGC 4 cut(s) 197, 200, 244, 437
PleI GAGTC 2 cut(s) 11, 242
PpsI GAGTC 2 cut(s) 11, 242
Psp124BI GAGCTC 1 cut(s) 738
Psp6I CCWGG 3 cut(s) 11, 66, 696
PspGI CCWGG 3 cut(s) 11, 66, 696
PspN4I GGNNCC 1 cut(s) 634
PspPI GGNCC 2 cut(s) 70, 92
PstI CTGCAG 1 cut(s) 440
RsaI GTAC 2 cut(s) 449, 759
RsaNI GTAC 2 cut(s) 448, 758
SacI GAGCTC 1 cut(s) 738
SacII CCGCGG 1 cut(s) 199
SalI GTCGAC 2 cut(s) 189, 212
SaqAI TTAA 1 cut(s) 618
SatI GCNGC 4 cut(s) 196, 199, 243, 436
Sau3AI GATC 2 cut(s) 34, 570
Sau96I GGNCC 2 cut(s) 70, 92
SchI GAGTC 2 cut(s) 11, 242
ScrFI CCNGG 3 cut(s) 13, 68, 698
SduI GDGCHC 2 cut(s) 321, 738
SetI ASST 7 cut(s) 62, 174, 375, 510, 738, 778, 796
SfaNI GCATC 3 cut(s) 514, 521, 778
SfcI CTRYAG 1 cut(s) 436
Sfr303I CCGCGG 1 cut(s) 199
SfuI TTCGAA 1 cut(s) 296
SgrBI CCGCGG 1 cut(s) 199
SgrDI CGTCGACG 1 cut(s) 212
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
Sse9I AATT 4 cut(s) 451, 485, 812, 826
SsiI CCGC 6 cut(s) 84, 105, 196, 198, 630, 767
SstI GAGCTC 1 cut(s) 738
StyD4I CCNGG 3 cut(s) 11, 66, 696
TaaI ACNGT 3 cut(s) 521, 762, 855
TaiI ACGT 1 cut(s) 174
TaqI TCGA 5 cut(s) 190, 213, 296, 455, 642
TasI AATT 4 cut(s) 451, 485, 812, 826
TatI WGTACW 1 cut(s) 757
TauI GCSGC 2 cut(s) 198, 201
TfiI GAWTC 1 cut(s) 298
Tru1I TTAA 1 cut(s) 618
Tru9I TTAA 1 cut(s) 618
TscAI CASTG 3 cut(s) 445, 526, 668
TseFI GTSAC 1 cut(s) 762
TseI GCWGC 2 cut(s) 242, 435
Tsp45I GTSAC 1 cut(s) 762
TspDTI ATGAA 2 cut(s) 256, 804
TspGWI ACGGA 1 cut(s) 52
TspRI CASTG 3 cut(s) 445, 526, 668
XapI RAATTY 1 cut(s) 826
XmiI GTMKAC 2 cut(s) 190, 213
XmnI GAANNNNTTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.