RchiOBHm_Chr1g0351531

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000446
Physical Location & Seq
Forward (+)
44812795 .. 44813750
956 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57729

Sequence Viewer

Length: 729 bp
ATGGCGGATGATAATCAAAGTGAGCTCCCTACGGCTACAGTTTTGATACTTCGCCAATCCGGATTCATTGGTCTCATGGAACCCAAACTCTCCCAAAAATTCAACTTACTGAAAGCCTGGGACTCTCCTCTCCCTCAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCCCTTATCTCCTCCGCCAATGGGCCGCCCATTACCACCCAAATCCTCCACTTGCTCCCCTCCCTCAAACTCATAGCCACCACCAGCGCCGGCATTGACCACGTTGACTTGGCCGAGTGTCGACGCCGCGGTGTTGCCGTCTCCAACGCCGGTAAAGCCTTTGCTGAGGACGCTGCTGACTCGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCAGTATGTTAGGGATGGGCTCTGGTCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTCGAAGTTGCAAAAAGATTGGAGGCATTTGGCTGCAGTGTGTCGTACAATTCGAGGAGCGAAAGGCCGTATCTGTCATACCAGTTCTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATCATCTGTTGCGCATTGACAGAGCAAACCCACCACATGATCAACAAGGAAGTTTTGTCTGCGTTGGGAAGAGAGGGGTGGTTGTTAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

242

Amino Acids

26.24

Weight (kDa)

6.52

Isoelectric Point (pI)

42.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 38 - 233 2.7e-17 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 122 - 240 1.1e-26 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 649
AccBSI CCGCTC 1 cut(s) 165
AccI GTMKAC 2 cut(s) 295, 431
AccII CGCG 1 cut(s) 303
AccIII TCCGGA 1 cut(s) 59
AciI CCGC 6 cut(s) 5, 163, 189, 200, 301, 303
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 1 cut(s) 98
AcyI GRCGYC 1 cut(s) 298
AfaI GTAC 1 cut(s) 554
AfiI CCNNNNNNNGG 1 cut(s) 195
AgsI TTSAA 1 cut(s) 103
AjnI CCWGG 2 cut(s) 116, 171
AluBI AGCT 2 cut(s) 25, 613
AluI AGCT 2 cut(s) 25, 613
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 3 cut(s) 77, 319, 435
Aor13HI TCCGGA 1 cut(s) 59
AoxI GGCC 4 cut(s) 174, 197, 285, 572
ApeKI GCWGC 3 cut(s) 347, 540, 617
ApoI RAATTY 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 391
AspLEI GCGC 2 cut(s) 263, 650
AspS9I GGNCC 2 cut(s) 175, 197
AsuII TTCGAA 2 cut(s) 401, 510
BanII GRGCYC 2 cut(s) 27, 426
Bbv12I GWGCWC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 339
BbvI GCAGC 3 cut(s) 334, 527, 629
BccI CCATC 1 cut(s) 413
BceAI ACGGC 3 cut(s) 48, 296, 559
BciT130I CCWGG 2 cut(s) 118, 173
BclI TGATCA 1 cut(s) 675
BcoDI GTCTC 3 cut(s) 77, 319, 435
BfmI CTRYAG 2 cut(s) 36, 541
BfoI RGCGCY 1 cut(s) 264
BisI GCNGC 5 cut(s) 200, 301, 348, 541, 618
BlsI GCNGC 5 cut(s) 201, 302, 349, 542, 619
Bme1390I CCNGG 2 cut(s) 118, 173
BmgT120I GGNCC 2 cut(s) 175, 197
BmiI GGNNCC 1 cut(s) 81
BmrFI CCNGG 2 cut(s) 118, 173
BmsI GCATC 1 cut(s) 619
Bpu10I CCTNAGC 1 cut(s) 339
Bpu14I TTCGAA 2 cut(s) 401, 510
BpuEI CTTGAG 1 cut(s) 120
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 298
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 117, 301
BsaWI WCCGGW 1 cut(s) 59
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bsc4I CCNNNNNNNGG 1 cut(s) 195
Bse118I RCCGGY 2 cut(s) 263, 323
Bse1I ACTGG 2 cut(s) 142, 589
Bse8I GATNNNNATC 1 cut(s) 12
BseAI TCCGGA 1 cut(s) 59
BseBI CCWGG 2 cut(s) 118, 173
BseDI CCNNGG 2 cut(s) 117, 301
BseGI GGATG 2 cut(s) 13, 424
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 195
BseMII CTCAG 1 cut(s) 330
BseNI ACTGG 2 cut(s) 142, 589
BseRI GAGGAG 4 cut(s) 117, 175, 453, 577
BseXI GCAGC 3 cut(s) 334, 527, 629
Bsh1236I CGCG 1 cut(s) 303
BshFI GGCC 4 cut(s) 176, 199, 287, 574
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 3 cut(s) 60, 264, 324
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 1 cut(s) 195
BsmAI GTCTC 3 cut(s) 77, 319, 435
BsmBI CGTCTC 1 cut(s) 319
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 4 cut(s) 176, 199, 287, 574
Bso31I GGTCTC 1 cut(s) 77
Bsp119I TTCGAA 2 cut(s) 401, 510
Bsp1286I GDGCHC 2 cut(s) 27, 426
Bsp13I TCCGGA 1 cut(s) 59
Bsp143I GATC 1 cut(s) 675
BspACI CCGC 6 cut(s) 5, 163, 189, 200, 301, 303
BspANI GGCC 4 cut(s) 176, 199, 287, 574
BspCNI CTCAG 1 cut(s) 331
BspEI TCCGGA 1 cut(s) 59
BspFNI CGCG 1 cut(s) 303
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 1 cut(s) 81
BspMAI CTGCAG 1 cut(s) 545
BspT104I TTCGAA 2 cut(s) 401, 510
BspTNI GGTCTC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 165
BsrFI RCCGGY 2 cut(s) 263, 323
BsrI ACTGG 2 cut(s) 142, 589
BssAI RCCGGY 2 cut(s) 263, 323
BssECI CCNNGG 2 cut(s) 117, 301
BssMI GATC 1 cut(s) 675
BssNI GRCGYC 1 cut(s) 298
Bst2UI CCWGG 2 cut(s) 118, 173
Bst4CI ACNGT 2 cut(s) 40, 626
Bst6I CTCTTC 1 cut(s) 700
BstACI GRCGYC 1 cut(s) 298
BstBI TTCGAA 2 cut(s) 401, 510
BstC8I GCNNGC 3 cut(s) 163, 265, 615
BstDEI CTNAG 2 cut(s) 339, 459
BstDSI CCRYGG 1 cut(s) 301
BstF5I GGATG 2 cut(s) 13, 424
BstFNI CGCG 1 cut(s) 303
BstH2I RGCGCY 1 cut(s) 264
BstHHI GCGC 2 cut(s) 263, 650
BstKTI GATC 1 cut(s) 678
BstMAI GTCTC 3 cut(s) 77, 319, 435
BstMBI GATC 1 cut(s) 675
BstMWI GCNNNNNNNGC 3 cut(s) 329, 344, 356
BstNI CCWGG 2 cut(s) 118, 173
BstSCI CCNGG 2 cut(s) 116, 171
BstSFI CTRYAG 2 cut(s) 36, 541
BstUI CGCG 1 cut(s) 303
BstV1I GCAGC 3 cut(s) 334, 527, 629
BsuRI GGCC 4 cut(s) 176, 199, 287, 574
BtgI CCRYGG 1 cut(s) 301
BtsCI GGATG 2 cut(s) 13, 424
BtsI GCAGTG 1 cut(s) 550
BtsIMutI CAGTG 2 cut(s) 550, 631
Cac8I GCNNGC 3 cut(s) 163, 265, 615
CciI TCATGA 1 cut(s) 381
CfoI GCGC 2 cut(s) 263, 650
Cfr10I RCCGGY 2 cut(s) 263, 323
Cfr13I GGNCC 2 cut(s) 175, 197
Cfr42I CCGCGG 1 cut(s) 304
CseI GACGC 2 cut(s) 306, 353
Csp6I GTAC 1 cut(s) 553
CviAII CATG 3 cut(s) 76, 382, 673
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 2 cut(s) 339, 459
DpnI GATC 1 cut(s) 677
DpnII GATC 1 cut(s) 675
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 700
EarI CTCTTC 1 cut(s) 700
EciI GGCGGA 2 cut(s) 20, 178
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 2 cut(s) 27, 426
Eco31I GGTCTC 1 cut(s) 77
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 116, 171
EcoT38I GRGCYC 2 cut(s) 27, 426
Esp3I CGTCTC 1 cut(s) 319
FaeI CATG 3 cut(s) 79, 385, 676
FaiI YATR 6 cut(s) 77, 248, 383, 411, 586, 674
FaqI GGGAC 1 cut(s) 134
FatI CATG 3 cut(s) 75, 381, 672
FauI CCCGC 1 cut(s) 170
FbaI TGATCA 1 cut(s) 675
FblI GTMKAC 2 cut(s) 295, 431
Fnu4HI GCNGC 5 cut(s) 200, 301, 348, 541, 618
FokI GGATG 2 cut(s) 20, 431
FriOI GRGCYC 2 cut(s) 27, 426
Fsp4HI GCNGC 5 cut(s) 200, 301, 348, 541, 618
FspI TGCGCA 1 cut(s) 649
GlaI GCGC 2 cut(s) 262, 649
GluI GCNGC 5 cut(s) 200, 301, 348, 541, 618
HaeII RGCGCY 1 cut(s) 264
HaeIII GGCC 4 cut(s) 176, 199, 287, 574
HapII CCGG 3 cut(s) 60, 264, 324
HgaI GACGC 2 cut(s) 306, 353
HhaI GCGC 2 cut(s) 263, 650
Hin1I GRCGYC 1 cut(s) 298
Hin1II CATG 3 cut(s) 79, 385, 676
Hin6I GCGC 2 cut(s) 261, 648
HinP1I GCGC 2 cut(s) 261, 648
HincII GTYRAC 2 cut(s) 280, 296
HindII GTYRAC 2 cut(s) 280, 296
HinfI GANTC 4 cut(s) 63, 122, 353, 403
HpaII CCGG 3 cut(s) 60, 264, 324
Hpy166II GTNNAC 3 cut(s) 280, 296, 432
Hpy188III TCNNGA 4 cut(s) 60, 137, 148, 382
Hpy8I GTNNAC 3 cut(s) 280, 296, 432
Hpy99I CGWCG 1 cut(s) 300
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 2 cut(s) 40, 626
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 3 cut(s) 518, 543, 617
HpyF10VI GCNNNNNNNGC 3 cut(s) 329, 344, 356
HpyF3I CTNAG 2 cut(s) 339, 459
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 298
Hsp92II CATG 3 cut(s) 79, 385, 676
HspAI GCGC 2 cut(s) 261, 648
Kpn2I TCCGGA 1 cut(s) 59
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Ksp22I TGATCA 1 cut(s) 675
KspI CCGCGG 1 cut(s) 304
Kzo9I GATC 1 cut(s) 675
LmnI GCTCC 4 cut(s) 30, 170, 234, 564
Lsp1109I GCAGC 3 cut(s) 334, 527, 629
LweI GCATC 1 cut(s) 619
MaeII ACGT 1 cut(s) 276
MalI GATC 1 cut(s) 677
MbiI CCGCTC 1 cut(s) 165
MboI GATC 1 cut(s) 675
MboII GAAGA 1 cut(s) 717
MhlI GDGCHC 2 cut(s) 27, 426
MluCI AATT 2 cut(s) 98, 556
MlyI GAGTC 2 cut(s) 116, 347
MmeI TCCRAC 2 cut(s) 342, 469
MroI TCCGGA 1 cut(s) 59
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 1 cut(s) 391
MseI TTAA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 303
MspI CCGG 3 cut(s) 60, 264, 324
MspR9I CCNGG 2 cut(s) 118, 173
MvaI CCWGG 2 cut(s) 118, 173
MvnI CGCG 1 cut(s) 303
MwoI GCNNNNNNNGC 3 cut(s) 329, 344, 356
NaeI GCCGGC 1 cut(s) 265
NdeII GATC 1 cut(s) 675
NgoMIV GCCGGC 1 cut(s) 263
NlaIII CATG 3 cut(s) 79, 385, 676
NlaIV GGNNCC 1 cut(s) 81
NmeAIII GCCGAG 2 cut(s) 313, 335
NsbI TGCGCA 1 cut(s) 649
NspV TTCGAA 2 cut(s) 401, 510
PagI TCATGA 1 cut(s) 381
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 557
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 391
PfeI GAWTC 2 cut(s) 63, 403
PkrI GCNGC 5 cut(s) 201, 302, 349, 542, 619
PleI GAGTC 2 cut(s) 116, 347
PpsI GAGTC 2 cut(s) 116, 347
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 116, 171
PspGI CCWGG 2 cut(s) 116, 171
PspN4I GGNNCC 1 cut(s) 81
PspPI GGNCC 2 cut(s) 175, 197
PstI CTGCAG 1 cut(s) 545
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
SacI GAGCTC 1 cut(s) 27
SacII CCGCGG 1 cut(s) 304
SalI GTCGAC 1 cut(s) 294
SaqAI TTAA 1 cut(s) 722
SatI GCNGC 5 cut(s) 200, 301, 348, 541, 618
Sau3AI GATC 1 cut(s) 675
Sau96I GGNCC 2 cut(s) 175, 197
SchI GAGTC 2 cut(s) 116, 347
ScrFI CCNGG 2 cut(s) 118, 173
SduI GDGCHC 2 cut(s) 27, 426
SetI ASST 4 cut(s) 27, 279, 480, 615
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 2 cut(s) 36, 541
Sfr303I CCGCGG 1 cut(s) 304
SfuI TTCGAA 2 cut(s) 401, 510
SgrBI CCGCGG 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 135
SmoI CTYRAG 1 cut(s) 135
Sse9I AATT 2 cut(s) 98, 556
SsiI CCGC 6 cut(s) 5, 163, 189, 200, 301, 303
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 116, 171
TaaI ACNGT 2 cut(s) 40, 626
TaiI ACGT 1 cut(s) 279
TaqI TCGA 4 cut(s) 295, 401, 510, 560
TasI AATT 2 cut(s) 98, 556
TauI GCSGC 2 cut(s) 202, 303
TfiI GAWTC 2 cut(s) 63, 403
Tru1I TTAA 1 cut(s) 722
Tru9I TTAA 1 cut(s) 722
TscAI CASTG 2 cut(s) 550, 631
TseI GCWGC 3 cut(s) 347, 540, 617
TspDTI ATGAA 2 cut(s) 55, 361
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 2 cut(s) 550, 631
XapI RAATTY 1 cut(s) 98
XmiI GTMKAC 2 cut(s) 295, 431
XmnI GAANNNNTTC 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.