RLG00000028402

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22269734 .. 22271245
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028402

Sequence Viewer

Length: 1008 bp
ATGGCCAGATTGCAATTCCGTGATGCCTATGAGGATGATGTACCATCCGAGGAACTCCCACATCTCCTACTCATTCATCCACCATCATCCGTTACACGTCTCCCATCTGAAATCTGTGAAAAGTTCCGTGTCCTGAAAGCATGGGAGTCGCCACTCCCTCTAGACCAGCTCCTGATCACCCATGCACGCTCAATCCAAGCCCTACTCATTCGCGGTTTTCTTACGATTGACACAGACATCCTTCAGCAGCTACCGGCACTGAAACTCATCATAACAATCAGCGTTGGTGTGAACGGCATTGACATGGCAGAGTGCCGGCGGCGTGGGATTTCTGTGACCAATGCGGGAAGTGTATACTCGGAAGATGTTGCTGATATTGCTGTTGGGTTGTACATTGATGTGCTTAGAAAACTTTCAGCAGCAGACCGGTATGTAAGACAAGGGCTTTGGAGTAGCAAAGGAGATTACCCTCTTGGTTCCAAGTTAGGTGGGAAGCGAGTTGGGATTGTTGGATTAGGAAGCATTGGCTCAGAAGTTGCTAAACGACTTGAGGCCTTTGGCTGCATCATCTCATACAATTCAAGGAGGGAAAAGCCAACTTCGCCATACCCCTTCTATTCTATCGTCTCTGAACTTGCAGCCAATAGTGATGCCCTCATCATCTGTTGTCCGTTAACAGAACAAACCCACCATATTATTAACAAGGAAGTCTTGGCTTCACTGGGAAGAGAGGGTGTGATTGTTAATGTAGCCCGCGGGGCAATAATTGACGAGAAAGAACTGGTGAAGTGTTTGGTGAGTGGGGAAATAAGAGGTGCTGGTTTTGATGTGTTTGAGAATGAGCCTCAAGTTCCTGAAGAGCTCTTTACATTGGACAATGTTGTTCTATCCCCACATACGGCTATTTTTACTCCGGAATCTTTGCAGAGTGTGAAAGAAGTACTAATCGCCAATGTGGAAGCTTTCTTCTTGAATAAACCTCTGGTTACTCCTGTCATGGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

336

Amino Acids

36.77

Weight (kDa)

5.24

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 55 - 332 1.8e-20 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 129 - 301 1.2e-52 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 354
AccII CGCG 2 cut(s) 213, 756
AccIII TCCGGA 1 cut(s) 913
AciI CCGC 5 cut(s) 213, 319, 344, 754, 756
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 227, 876
AfaI GTAC 3 cut(s) 42, 392, 942
AfiI CCNNNNNNNGG 1 cut(s) 898
AflIII ACRYGT 1 cut(s) 95
AgeI ACCGGT 1 cut(s) 426
AgsI TTSAA 2 cut(s) 582, 973
AjiI CACGTC 1 cut(s) 98
AluBI AGCT 4 cut(s) 169, 250, 862, 962
AluI AGCT 4 cut(s) 169, 250, 862, 962
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 2 cut(s) 104, 631
AlwNI CAGNNNCTG 1 cut(s) 172
Aor13HI TCCGGA 1 cut(s) 913
AoxI GGCC 2 cut(s) 3, 552
ApeKI GCWGC 4 cut(s) 247, 419, 561, 638
ArsI GACNNNNNNTTYG 2 cut(s) 429, 461
AsiGI ACCGGT 1 cut(s) 426
Asp700I GAANNNNTTC 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 169, 796, 808
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 864
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 4 cut(s) 259, 431, 548, 650
BccI CCATC 3 cut(s) 52, 91, 112
BceAI ACGGC 2 cut(s) 310, 915
BcgI CGANNNNNNTGC 2 cut(s) 129, 163
BclI TGATCA 1 cut(s) 174
BcoDI GTCTC 2 cut(s) 104, 631
BfaI CTAG 1 cut(s) 161
BglI GCCNNNNNGGC 1 cut(s) 758
BisI GCNGC 5 cut(s) 248, 320, 420, 562, 639
BlsI GCNGC 5 cut(s) 249, 321, 421, 563, 640
BmcAI AGTACT 1 cut(s) 942
BmgBI CACGTC 1 cut(s) 98
BmiI GGNNCC 1 cut(s) 478
BmrI ACTGGG 1 cut(s) 731
BmsI GCATC 3 cut(s) 13, 573, 640
BmuI ACTGGG 1 cut(s) 731
BpuEI CTTGAG 2 cut(s) 569, 831
BsaJI CCNNGG 2 cut(s) 48, 754
BsaWI WCCGGW 2 cut(s) 426, 913
Bsc4I CCNNNNNNNGG 1 cut(s) 898
Bse118I RCCGGY 3 cut(s) 253, 315, 426
Bse1I ACTGG 2 cut(s) 726, 786
BseAI TCCGGA 1 cut(s) 913
BseDI CCNNGG 2 cut(s) 48, 754
BseGI GGATG 6 cut(s) 40, 44, 76, 86, 237, 1006
BseLI CCNNNNNNNGG 1 cut(s) 898
BseMII CTCAG 1 cut(s) 543
BseNI ACTGG 2 cut(s) 726, 786
BseXI GCAGC 4 cut(s) 259, 431, 548, 650
Bsh1236I CGCG 2 cut(s) 213, 756
BshFI GGCC 2 cut(s) 5, 554
BshTI ACCGGT 1 cut(s) 426
BsiHKAI GWGCWC 1 cut(s) 864
BsiSI CCGG 4 cut(s) 254, 316, 427, 914
BslI CCNNNNNNNGG 1 cut(s) 898
BsmAI GTCTC 2 cut(s) 104, 631
BsmBI CGTCTC 2 cut(s) 104, 631
BsnI GGCC 2 cut(s) 5, 554
Bsp1286I GDGCHC 1 cut(s) 864
Bsp13I TCCGGA 1 cut(s) 913
Bsp1407I TGTACA 1 cut(s) 390
Bsp143I GATC 1 cut(s) 174
BspACI CCGC 5 cut(s) 213, 319, 344, 754, 756
BspANI GGCC 2 cut(s) 5, 554
BspCNI CTCAG 1 cut(s) 542
BspEI TCCGGA 1 cut(s) 913
BspFNI CGCG 2 cut(s) 213, 756
BspLI GGNNCC 1 cut(s) 478
BspQI GCTCTTC 1 cut(s) 852
BsrFI RCCGGY 3 cut(s) 253, 315, 426
BsrGI TGTACA 1 cut(s) 390
BsrI ACTGG 2 cut(s) 726, 786
BssAI RCCGGY 3 cut(s) 253, 315, 426
BssECI CCNNGG 2 cut(s) 48, 754
BssMI GATC 1 cut(s) 174
BssNAI GTATAC 1 cut(s) 355
Bst1107I GTATAC 1 cut(s) 355
Bst6I CTCTTC 2 cut(s) 721, 852
BstAUI TGTACA 1 cut(s) 390
BstC8I GCNNGC 3 cut(s) 187, 317, 754
BstDEI CTNAG 2 cut(s) 404, 529
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 6 cut(s) 40, 44, 76, 86, 237, 1006
BstFNI CGCG 2 cut(s) 213, 756
BstKTI GATC 1 cut(s) 177
BstMAI GTCTC 2 cut(s) 104, 631
BstMBI GATC 1 cut(s) 174
BstMWI GCNNNNNNNGC 3 cut(s) 377, 601, 758
BstUI CGCG 2 cut(s) 213, 756
BstV1I GCAGC 4 cut(s) 259, 431, 548, 650
BstZ17I GTATAC 1 cut(s) 355
BsuRI GGCC 2 cut(s) 5, 554
BtgI CCRYGG 1 cut(s) 754
BtrI CACGTC 1 cut(s) 98
BtsCI GGATG 6 cut(s) 40, 44, 76, 86, 237, 1006
BtsIMutI CAGTG 2 cut(s) 257, 719
Cac8I GCNNGC 3 cut(s) 187, 317, 754
CaiI CAGNNNCTG 1 cut(s) 172
Cfr10I RCCGGY 3 cut(s) 253, 315, 426
Cfr42I CCGCGG 1 cut(s) 757
Csp6I GTAC 3 cut(s) 41, 391, 941
CspAI ACCGGT 1 cut(s) 426
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 4 cut(s) 141, 182, 304, 997
CviQI GTAC 3 cut(s) 41, 391, 941
DdeI CTNAG 2 cut(s) 404, 529
DpnI GATC 1 cut(s) 176
DpnII GATC 1 cut(s) 174
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 721, 852
EarI CTCTTC 2 cut(s) 721, 852
Ecl136II GAGCTC 1 cut(s) 862
Eco147I AGGCCT 1 cut(s) 554
Eco24I GRGCYC 1 cut(s) 864
Eco53kI GAGCTC 1 cut(s) 862
Eco57I CTGAAG 2 cut(s) 227, 876
EcoICRI GAGCTC 1 cut(s) 862
EcoT38I GRGCYC 1 cut(s) 864
Esp3I CGTCTC 2 cut(s) 104, 631
FaeI CATG 4 cut(s) 144, 185, 307, 1000
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FatI CATG 4 cut(s) 140, 181, 303, 996
FauI CCCGC 3 cut(s) 337, 749, 761
FbaI TGATCA 1 cut(s) 174
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 5 cut(s) 248, 320, 420, 562, 639
FokI GGATG 5 cut(s) 31, 47, 63, 73, 224
FriOI GRGCYC 1 cut(s) 864
Fsp4HI GCNGC 5 cut(s) 248, 320, 420, 562, 639
FspBI CTAG 1 cut(s) 161
GluI GCNGC 5 cut(s) 248, 320, 420, 562, 639
HaeIII GGCC 2 cut(s) 5, 554
HapII CCGG 4 cut(s) 254, 316, 427, 914
Hin1II CATG 4 cut(s) 144, 185, 307, 1000
HincII GTYRAC 1 cut(s) 675
HindII GTYRAC 1 cut(s) 675
HindIII AAGCTT 1 cut(s) 960
HinfI GANTC 2 cut(s) 146, 917
HpaI GTTAAC 1 cut(s) 675
HpaII CCGG 4 cut(s) 254, 316, 427, 914
HphI GGTGA 3 cut(s) 169, 796, 808
Hpy166II GTNNAC 3 cut(s) 292, 355, 675
Hpy188I TCNGA 5 cut(s) 49, 109, 361, 532, 631
Hpy188III TCNNGA 6 cut(s) 133, 161, 172, 854, 914, 970
Hpy8I GTNNAC 3 cut(s) 292, 355, 675
HpyAV CCTTC 2 cut(s) 251, 622
HpyCH4IV ACGT 1 cut(s) 97
HpyCH4V TGCA 5 cut(s) 13, 185, 564, 638, 925
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 601, 758
HpyF3I CTNAG 2 cut(s) 404, 529
HpySE526I ACGT 1 cut(s) 97
Hsp92II CATG 4 cut(s) 144, 185, 307, 1000
Kpn2I TCCGGA 1 cut(s) 913
KroI GCCGGC 1 cut(s) 315
KroNI GCCGGC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 174
KspAI GTTAAC 1 cut(s) 675
KspI CCGCGG 1 cut(s) 757
Kzo9I GATC 1 cut(s) 174
LguI GCTCTTC 1 cut(s) 852
LmnI GCTCC 1 cut(s) 174
Lsp1109I GCAGC 4 cut(s) 259, 431, 548, 650
LweI GCATC 3 cut(s) 13, 573, 640
MaeI CTAG 1 cut(s) 161
MaeII ACGT 1 cut(s) 97
MaeIII GTNAC 3 cut(s) 91, 334, 985
MalI GATC 1 cut(s) 176
MboI GATC 1 cut(s) 174
MboII GAAGA 4 cut(s) 374, 738, 869, 958
MhlI GDGCHC 1 cut(s) 864
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 14, 577, 765
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 490
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 913
MroNI GCCGGC 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 412
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 674, 699, 744
MslI CAYNNNNRTG 2 cut(s) 302, 398
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 756
MspI CCGG 4 cut(s) 254, 316, 427, 914
MvnI CGCG 2 cut(s) 213, 756
MwoI GCNNNNNNNGC 3 cut(s) 377, 601, 758
NaeI GCCGGC 1 cut(s) 317
NdeII GATC 1 cut(s) 174
NgoMIV GCCGGC 1 cut(s) 315
NlaIII CATG 4 cut(s) 144, 185, 307, 1000
NlaIV GGNNCC 1 cut(s) 478
NmuCI GTSAC 1 cut(s) 334
PceI AGGCCT 1 cut(s) 554
PciSI GCTCTTC 1 cut(s) 852
PdiI GCCGGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 412
PfeI GAWTC 1 cut(s) 917
PinAI ACCGGT 1 cut(s) 426
PkrI GCNGC 5 cut(s) 249, 321, 421, 563, 640
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 864
PspN4I GGNNCC 1 cut(s) 478
PstNI CAGNNNCTG 1 cut(s) 172
RsaI GTAC 3 cut(s) 42, 392, 942
RsaNI GTAC 3 cut(s) 41, 391, 941
RseI CAYNNNNRTG 2 cut(s) 302, 398
SacI GAGCTC 1 cut(s) 864
SacII CCGCGG 1 cut(s) 757
SapI GCTCTTC 1 cut(s) 852
SaqAI TTAA 3 cut(s) 674, 699, 744
SatI GCNGC 5 cut(s) 248, 320, 420, 562, 639
Sau3AI GATC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 942
SchI GAGTC 1 cut(s) 155
SduI GDGCHC 1 cut(s) 864
SetI ASST 8 cut(s) 100, 171, 252, 490, 817, 864, 964, 982
SfaNI GCATC 3 cut(s) 13, 573, 640
Sfr303I CCGCGG 1 cut(s) 757
SgrBI CCGCGG 1 cut(s) 757
SmiMI CAYNNNNRTG 2 cut(s) 302, 398
SmlI CTYRAG 2 cut(s) 548, 846
SmoI CTYRAG 2 cut(s) 548, 846
Sse9I AATT 3 cut(s) 14, 577, 765
SseBI AGGCCT 1 cut(s) 554
SsiI CCGC 5 cut(s) 213, 319, 344, 754, 756
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 864
StuI AGGCCT 1 cut(s) 554
TaiI ACGT 1 cut(s) 100
TasI AATT 3 cut(s) 14, 577, 765
TatI WGTACW 2 cut(s) 390, 940
TauI GCSGC 1 cut(s) 322
TfiI GAWTC 1 cut(s) 917
Tru1I TTAA 3 cut(s) 674, 699, 744
Tru9I TTAA 3 cut(s) 674, 699, 744
TscAI CASTG 2 cut(s) 264, 726
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 4 cut(s) 247, 419, 561, 638
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 65
TspGWI ACGGA 4 cut(s) 8, 79, 116, 660
TspRI CASTG 2 cut(s) 264, 726
XbaI TCTAGA 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 354
XmnI GAANNNNTTC 1 cut(s) 412
XspI CTAG 1 cut(s) 161
ZrmI AGTACT 1 cut(s) 942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.