Rh1DG230700

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000446
Physical Location & Seq
Forward (+)
44306557 .. 44315708
9152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG230700.1

Sequence Viewer

Length: 981 bp
ATGGCAGATGATGATCACAGTGAGCTCCCTACAGTTCTTGTACTTCGCCGGTGCCCATTCATTGCTCTCTTGGAACCCAAATGCTCCCAGAAGTTCAACTTACTAAAAGCCTGGGACTCTCCTCTCCCTCAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCCCTCCTCTCCTCCGGCAATGGGCCTCCCATCACCGCCCAAATCCTCCAGTTTCTCCCCTCCCTCAAACTCGTGGTCACCAACAGCACCGGCCTTGACCACGTTGACTTGGCCGAGTGTCGACGCCGCGGCATTGCGGTCGCCAACGCCGCTAAAGTGTTTGCTGAGGACGTTGCTGACACGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGGTTTCGGCTTCGAATCGGTATGTTAGGGGTGGGCTGTGGGCTTGCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTTGGTATTGTTGGATTGGGCAGCATTGGGTTAGAAGTTGCAAAGAGATTGGAGGCATTTGGCTGCAGTGTCTCGTACAATTCAAGGAGCGAAAAGCCGTCTTTGTCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATCATCTGTTGTGGACTGACAGAGGAAACCCACCACATGATCAACAAGGAAGTTTTGTCTGCGTTGGGAAGAGAGGGGGTGATCGTTAATGTAGGGCGTGGAGCTATTATCGATGAGAAGGAAATGATCGAGTGTTTGGTGCGAGGAGAGATCGGAGGTGCTGGCTTGGATGTGTTCGAGAACGAGCCTAATGTTCCTAAAGAGCTCTTTTCATTGGATAATGTTGTACTGTCACCGCATCAAGGTATTGTTACACATGAAGCTTTTGTGGATTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCTGGCTGTGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

326

Amino Acids

35.13

Weight (kDa)

5.34

Isoelectric Point (pI)

35.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 41 - 321 8.6e-20 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 2.6e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 289
AccII CGCG 1 cut(s) 297
AciI CCGC 7 cut(s) 157, 204, 295, 297, 305, 318, 866
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 925
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 3 cut(s) 42, 548, 858
AfiI CCNNNNNNNGG 2 cut(s) 189, 872
AgsI TTSAA 2 cut(s) 97, 555
AjnI CCWGG 2 cut(s) 110, 165
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 5 cut(s) 25, 607, 734, 835, 893
AluI AGCT 5 cut(s) 25, 607, 734, 835, 893
Alw21I GWGCWC 2 cut(s) 27, 837
Alw26I GTCTC 2 cut(s) 429, 547
AoxI GGCC 4 cut(s) 168, 191, 259, 279
ApeKI GCWGC 3 cut(s) 492, 534, 611
ApoI RAATTY 1 cut(s) 925
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 385
AspS9I GGNCC 2 cut(s) 169, 191
AsuHPI GGTGA 5 cut(s) 193, 238, 721, 855, 920
AsuII TTCGAA 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 51
BanII GRGCYC 2 cut(s) 27, 837
BauI CACGAG 1 cut(s) 239
Bbv12I GWGCWC 2 cut(s) 27, 837
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 504, 521, 623
BccI CCATC 1 cut(s) 206
BceAI ACGGC 2 cut(s) 366, 553
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 2 cut(s) 112, 167
BclI TGATCA 2 cut(s) 13, 669
BcoDI GTCTC 2 cut(s) 429, 547
BfmI CTRYAG 2 cut(s) 30, 535
BisI GCNGC 6 cut(s) 295, 298, 318, 493, 535, 612
BlsI GCNGC 6 cut(s) 296, 299, 319, 494, 536, 613
Bme1390I CCNGG 2 cut(s) 112, 167
BmgT120I GGNCC 2 cut(s) 169, 191
BmiI GGNNCC 2 cut(s) 53, 75
BmrFI CCNGG 2 cut(s) 112, 167
BmsI GCATC 2 cut(s) 613, 877
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 111, 295
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 872
Bse118I RCCGGY 2 cut(s) 48, 257
Bse1I ACTGG 2 cut(s) 136, 217
Bse3DI GCAATG 3 cut(s) 60, 193, 300
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 112, 167
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 1 cut(s) 805
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 189, 872
BseMI GCAATG 3 cut(s) 60, 193, 300
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 136, 217
BseRI GAGGAG 5 cut(s) 111, 164, 169, 447, 789
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 3 cut(s) 504, 521, 623
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 4 cut(s) 170, 193, 261, 281
BshNI GGYRCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 2 cut(s) 27, 837
BsiSI CCGG 3 cut(s) 49, 183, 258
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 189, 872
BsmAI GTCTC 2 cut(s) 429, 547
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 4 cut(s) 170, 193, 261, 281
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 27, 56, 837
Bsp143I GATC 5 cut(s) 13, 669, 711, 756, 780
BspACI CCGC 7 cut(s) 157, 204, 295, 297, 305, 318, 866
BspANI GGCC 4 cut(s) 170, 193, 261, 281
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 53, 75
BspMAI CTGCAG 1 cut(s) 539
BspT104I TTCGAA 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 3 cut(s) 60, 193, 300
BsrFI RCCGGY 2 cut(s) 48, 257
BsrI ACTGG 2 cut(s) 136, 217
BssAI RCCGGY 2 cut(s) 48, 257
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 5 cut(s) 13, 669, 711, 756, 780
BssNI GRCGYC 1 cut(s) 292
BssSI CACGAG 1 cut(s) 239
Bst2BI CACGAG 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 112, 167
Bst4CI ACNGT 5 cut(s) 20, 34, 620, 861, 954
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 4 cut(s) 157, 427, 609, 793
BstDEI CTNAG 2 cut(s) 333, 453
BstDSI CCRYGG 1 cut(s) 295
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 1 cut(s) 805
BstFNI CGCG 1 cut(s) 297
BstKTI GATC 5 cut(s) 16, 672, 714, 759, 783
BstMAI GTCTC 2 cut(s) 429, 547
BstMBI GATC 5 cut(s) 13, 669, 711, 756, 780
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 317, 350
BstNI CCWGG 2 cut(s) 112, 167
BstPI GGTNACC 1 cut(s) 244
BstSCI CCNGG 2 cut(s) 110, 165
BstSFI CTRYAG 2 cut(s) 30, 535
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 504, 521, 623
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 4 cut(s) 170, 193, 261, 281
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 805
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 3 cut(s) 25, 544, 625
Cac8I GCNNGC 4 cut(s) 157, 427, 609, 793
CciI TCATGA 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 48, 257
Cfr13I GGNCC 2 cut(s) 169, 191
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 1 cut(s) 300
Csp6I GTAC 3 cut(s) 41, 547, 857
CviAII CATG 3 cut(s) 376, 667, 887
CviQI GTAC 3 cut(s) 41, 547, 857
DdeI CTNAG 2 cut(s) 333, 453
DpnI GATC 5 cut(s) 15, 671, 713, 758, 782
DpnII GATC 5 cut(s) 13, 669, 711, 756, 780
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Ecl136II GAGCTC 2 cut(s) 25, 835
Eco24I GRGCYC 2 cut(s) 27, 837
Eco53kI GAGCTC 2 cut(s) 25, 835
Eco91I GGTNACC 1 cut(s) 244
EcoICRI GAGCTC 2 cut(s) 25, 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoO65I GGTNACC 1 cut(s) 244
EcoRII CCWGG 2 cut(s) 110, 165
EcoT38I GRGCYC 2 cut(s) 27, 837
FaeI CATG 3 cut(s) 379, 670, 890
FaiI YATR 5 cut(s) 377, 405, 580, 668, 888
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 375, 666, 886
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 2 cut(s) 13, 669
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 6 cut(s) 295, 298, 318, 493, 535, 612
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 2 cut(s) 27, 837
Fsp4HI GCNGC 6 cut(s) 295, 298, 318, 493, 535, 612
GluI GCNGC 6 cut(s) 295, 298, 318, 493, 535, 612
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 4 cut(s) 170, 193, 261, 281
HapII CCGG 3 cut(s) 49, 183, 258
HgaI GACGC 1 cut(s) 300
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 3 cut(s) 379, 670, 890
HincII GTYRAC 2 cut(s) 274, 290
HindII GTYRAC 2 cut(s) 274, 290
HindIII AAGCTT 1 cut(s) 891
HinfI GANTC 2 cut(s) 116, 397
HpaII CCGG 3 cut(s) 49, 183, 258
HphI GGTGA 5 cut(s) 193, 238, 721, 855, 920
Hpy166II GTNNAC 3 cut(s) 274, 290, 644
Hpy188I TCNGA 1 cut(s) 785
Hpy188III TCNNGA 4 cut(s) 131, 142, 376, 808
Hpy8I GTNNAC 3 cut(s) 274, 290, 644
Hpy99I CGWCG 1 cut(s) 294
HpyAV CCTTC 2 cut(s) 463, 742
HpyCH4III ACNGT 5 cut(s) 20, 34, 620, 861, 954
HpyCH4IV ACGT 2 cut(s) 270, 339
HpyCH4V TGCA 4 cut(s) 429, 512, 537, 611
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 350
HpyF3I CTNAG 2 cut(s) 333, 453
HpySE526I ACGT 2 cut(s) 270, 339
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 3 cut(s) 379, 670, 890
Ksp22I TGATCA 2 cut(s) 13, 669
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 5 cut(s) 13, 669, 711, 756, 780
LmnI GCTCC 5 cut(s) 30, 89, 164, 558, 731
Lsp1109I GCAGC 3 cut(s) 504, 521, 623
LweI GCATC 2 cut(s) 613, 877
MaeII ACGT 2 cut(s) 270, 339
MaeIII GTNAC 4 cut(s) 244, 861, 880, 916
MalI GATC 5 cut(s) 15, 671, 713, 758, 782
MbiI CCGCTC 1 cut(s) 159
MboI GATC 5 cut(s) 13, 669, 711, 756, 780
MboII GAAGA 2 cut(s) 711, 931
MhlI GDGCHC 3 cut(s) 27, 56, 837
MluCI AATT 5 cut(s) 550, 584, 911, 925, 976
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 2 cut(s) 717, 975
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 3 cut(s) 49, 183, 258
MspR9I CCNGG 2 cut(s) 112, 167
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 2 cut(s) 112, 167
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 317, 350
NdeII GATC 5 cut(s) 13, 669, 711, 756, 780
NlaIII CATG 3 cut(s) 379, 670, 890
NlaIV GGNNCC 2 cut(s) 53, 75
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 2 cut(s) 244, 861
NspV TTCGAA 1 cut(s) 395
OliI CACNNNNGTG 1 cut(s) 353
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 6 cut(s) 296, 299, 319, 494, 536, 613
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 2 cut(s) 27, 837
Psp6I CCWGG 2 cut(s) 110, 165
PspEI GGTNACC 1 cut(s) 244
PspGI CCWGG 2 cut(s) 110, 165
PspN4I GGNNCC 2 cut(s) 53, 75
PspPI GGNCC 2 cut(s) 169, 191
PstI CTGCAG 1 cut(s) 539
RsaI GTAC 3 cut(s) 42, 548, 858
RsaNI GTAC 3 cut(s) 41, 547, 857
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 2 cut(s) 27, 837
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 717, 975
SatI GCNGC 6 cut(s) 295, 298, 318, 493, 535, 612
Sau3AI GATC 5 cut(s) 13, 669, 711, 756, 780
Sau96I GGNCC 2 cut(s) 169, 191
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 112, 167
SduI GDGCHC 3 cut(s) 27, 56, 837
SfaNI GCATC 2 cut(s) 613, 877
SfcI CTRYAG 2 cut(s) 30, 535
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 395
SgrAI CRCCGGYG 1 cut(s) 48
SgrBI CCGCGG 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 5 cut(s) 550, 584, 911, 925, 976
SsiI CCGC 7 cut(s) 157, 204, 295, 297, 305, 318, 866
SstI GAGCTC 2 cut(s) 27, 837
StyD4I CCNGG 2 cut(s) 110, 165
TaaI ACNGT 5 cut(s) 20, 34, 620, 861, 954
TaiI ACGT 2 cut(s) 273, 342
TaqI TCGA 5 cut(s) 289, 395, 741, 759, 807
TasI AATT 5 cut(s) 550, 584, 911, 925, 976
TatI WGTACW 2 cut(s) 40, 856
TauI GCSGC 3 cut(s) 297, 300, 320
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 2 cut(s) 717, 975
Tru9I TTAA 2 cut(s) 717, 975
TscAI CASTG 3 cut(s) 25, 544, 625
TseFI GTSAC 2 cut(s) 244, 861
TseI GCWGC 3 cut(s) 492, 534, 611
Tsp45I GTSAC 2 cut(s) 244, 861
TspDTI ATGAA 4 cut(s) 49, 355, 831, 903
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 3 cut(s) 25, 544, 625
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.