Rorug01G0218400

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000446
Physical Location & Seq
Reverse (-)
31260157 .. 31264341
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0218400.1

Sequence Viewer

Length: 945 bp
ATGGTGAGGAGGGCTCTGATTCTCAATTCCTTTTCTCTCTCTCTCTGGTTGAAAAGGTTATCAAGGGCTGCGTTAAAGATTGGGAAGCTGTTTGCATCAAATGGCGGCCTTGAATATTTTAAGCAGTGTGTGGAGGTGTTTAGGACAAAAGTTGACGAGGTGGAACAGTGTAAAGCTCAACTGCAGCAAGCAATTACTAATTGTGAAGCAGAGTTTGTAGACATATGTGCTGTCCTGGGTGAGAAACCACCACATTTTGATCATAATATCAGTGGAAGTTTGAAGAAAGAGCTTGAAATCATTATCCCACAATTGGAGGTCATGAAGAAGCGGAAAATTGAAAGGAAGGACCAATTGTTTTCTGTCTTGGATCAGTTACAGAGACTTTCGACAGAAATAAGCAGGTCCTTGGAAGATAATCTGTACAAAATGGTGGTGGAAGAGACCGACTTGTCCTTGAAAAGGCTAGAAGAACTACATAGGCAGTTGCTTGAATATCAAGATGAGAAGAGGAACCGTTTGAAGCTGATAATGGACCACATGAGCATGCTAAACTCCCTGTGCTTGGTTCTTGGTATGGATTTTAAACACACAATTCATGAAATCCACCCCACTTTAGATGATCTTAATGGAGAAAAAGACGTAACGAACAGCACAATCGAGGGTTTGGCTAATTCGGTTCAGATATTGAGAGAGGTCAAGATACAGAGATGGCAGAGGCTTCAAACTTTTGCAAGTGCCCTATTGGAGATGTGGAATCTGATGGATACAACCATGGAGGAGCAAAAGAAATATCAGAATGTAACTAGTCGCATAGCTGCTTCAGAATCTGAAATCACTGAGCCTAACATTCTTTATGTAGACCTTCTAAATGATGTTGAAGCTGAAGTGTCAAAGTTGGAGCAGCTCAAGTCAATTAAGCTCAAAGAGATCCTTCTGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

314

Amino Acids

36.48

Weight (kDa)

5.91

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAP65_ASE1 PF03999 41 - 312 4.3e-36 Microtubule associated protein (MAP65/ASE1 family)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 451
Acc36I ACCTGC 1 cut(s) 393
AccI GTMKAC 2 cut(s) 219, 861
AciI CCGC 2 cut(s) 105, 331
AclWI GGATC 2 cut(s) 378, 925
AcuI CTGAAG 2 cut(s) 807, 906
AfaI GTAC 1 cut(s) 425
AfiI CCNNNNNNNGG 3 cut(s) 313, 462, 565
AhlI ACTAGT 1 cut(s) 806
AjnI CCWGG 1 cut(s) 234
AluBI AGCT 8 cut(s) 88, 176, 292, 526, 818, 884, 907, 922
AluI AGCT 8 cut(s) 88, 176, 292, 526, 818, 884, 907, 922
Alw26I GTCTC 2 cut(s) 376, 437
AlwI GGATC 2 cut(s) 378, 925
AlwNI CAGNNNCTG 1 cut(s) 830
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 4 cut(s) 68, 184, 818, 904
AspS9I GGNCC 3 cut(s) 349, 405, 535
AsuHPI GGTGA 2 cut(s) 16, 251
AvaII GGWCC 3 cut(s) 349, 405, 535
BaeGI GKGCMC 1 cut(s) 742
BanII GRGCYC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 55, 196, 805, 916
BccI CCATC 2 cut(s) 705, 757
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 760
BclI TGATCA 1 cut(s) 259
BcoDI GTCTC 2 cut(s) 376, 437
BcuI ACTAGT 1 cut(s) 806
BfaI CTAG 2 cut(s) 467, 807
BfmI CTRYAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 393
BfuI GTATCC 1 cut(s) 760
BisI GCNGC 5 cut(s) 69, 106, 185, 819, 905
BlsI GCNGC 5 cut(s) 70, 107, 186, 820, 906
Bme1390I CCNGG 1 cut(s) 236
Bme18I GGWCC 3 cut(s) 349, 405, 535
BmgT120I GGNCC 3 cut(s) 349, 405, 535
BmiI GGNNCC 1 cut(s) 515
BmrFI CCNGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 104
BplI GAGNNNNNCTC 1 cut(s) 30
BpuEI CTTGAG 1 cut(s) 893
BsaI GGTCTC 1 cut(s) 437
BsaJI CCNNGG 3 cut(s) 235, 408, 774
Bsc4I CCNNNNNNNGG 3 cut(s) 313, 462, 565
BseBI CCWGG 1 cut(s) 236
BseDI CCNNGG 3 cut(s) 235, 408, 774
BseLI CCNNNNNNNGG 3 cut(s) 313, 462, 565
BseMII CTCAG 1 cut(s) 831
BseRI GAGGAG 2 cut(s) 22, 794
BseSI GKGCMC 1 cut(s) 742
BseXI GCAGC 4 cut(s) 55, 196, 805, 916
BshFI GGCC 1 cut(s) 108
BslI CCNNNNNNNGG 3 cut(s) 313, 462, 565
BsmAI GTCTC 2 cut(s) 376, 437
BsnI GGCC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 437
Bsp1286I GDGCHC 2 cut(s) 16, 742
Bsp1407I TGTACA 1 cut(s) 423
Bsp143I GATC 4 cut(s) 259, 370, 622, 930
Bsp19I CCATGG 1 cut(s) 774
BspACI CCGC 2 cut(s) 105, 331
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 832
BspHI TCATGA 2 cut(s) 321, 598
BspLI GGNNCC 1 cut(s) 515
BspMAI CTGCAG 1 cut(s) 186
BspMI ACCTGC 1 cut(s) 393
BspPI GGATC 2 cut(s) 378, 925
BspTNI GGTCTC 1 cut(s) 437
BsrGI TGTACA 1 cut(s) 423
BssECI CCNNGG 3 cut(s) 235, 408, 774
BssMI GATC 4 cut(s) 259, 370, 622, 930
BssT1I CCWWGG 2 cut(s) 408, 774
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 2 cut(s) 168, 518
Bst6I CTCTTC 2 cut(s) 435, 503
BstAUI TGTACA 1 cut(s) 423
BstC8I GCNNGC 2 cut(s) 189, 548
BstDEI CTNAG 1 cut(s) 840
BstDSI CCRYGG 1 cut(s) 774
BstENI CCTNNNNNAGG 1 cut(s) 460
BstKTI GATC 4 cut(s) 262, 373, 625, 933
BstMAI GTCTC 2 cut(s) 376, 437
BstMBI GATC 4 cut(s) 259, 370, 622, 930
BstNI CCWGG 1 cut(s) 236
BstNSI RCATGY 1 cut(s) 550
BstSCI CCNGG 1 cut(s) 234
BstSFI CTRYAG 1 cut(s) 182
BstSLI GKGCMC 1 cut(s) 742
BstV1I GCAGC 4 cut(s) 55, 196, 805, 916
BstX2I RGATCY 1 cut(s) 930
BstYI RGATCY 1 cut(s) 930
BsuI GTATCC 1 cut(s) 760
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 774
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 4 cut(s) 131, 173, 277, 837
BveI ACCTGC 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 189, 548
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 2 cut(s) 321, 598
Cfr13I GGNCC 3 cut(s) 349, 405, 535
Csp6I GTAC 1 cut(s) 424
CviAII CATG 5 cut(s) 322, 541, 547, 599, 775
CviQI GTAC 1 cut(s) 424
DdeI CTNAG 1 cut(s) 840
DpnI GATC 4 cut(s) 261, 372, 624, 932
DpnII GATC 4 cut(s) 259, 370, 622, 930
DraI TTTAAA 1 cut(s) 586
DrdI GACNNNNNNGTC 1 cut(s) 451
DseDI GACNNNNNNGTC 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 435, 503
EarI CTCTTC 2 cut(s) 435, 503
Eco130I CCWWGG 2 cut(s) 408, 774
Eco24I GRGCYC 1 cut(s) 16
Eco31I GGTCTC 1 cut(s) 437
Eco47I GGWCC 3 cut(s) 349, 405, 535
Eco57I CTGAAG 2 cut(s) 807, 906
EcoNI CCTNNNNNAGG 1 cut(s) 460
EcoO109I RGGNCCY 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 408, 774
EcoT38I GRGCYC 1 cut(s) 16
ErhI CCWWGG 2 cut(s) 408, 774
FaeI CATG 5 cut(s) 325, 544, 550, 602, 778
FalI AAGNNNNNCTT 1 cut(s) 918
FatI CATG 5 cut(s) 321, 540, 546, 598, 774
FauNDI CATATG 1 cut(s) 224
FbaI TGATCA 1 cut(s) 259
FblI GTMKAC 2 cut(s) 219, 861
Fnu4HI GCNGC 5 cut(s) 69, 106, 185, 819, 905
FriOI GRGCYC 1 cut(s) 16
Fsp4HI GCNGC 5 cut(s) 69, 106, 185, 819, 905
FspBI CTAG 2 cut(s) 467, 807
GluI GCNGC 5 cut(s) 69, 106, 185, 819, 905
HaeIII GGCC 1 cut(s) 108
Hin1II CATG 5 cut(s) 325, 544, 550, 602, 778
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 3 cut(s) 19, 757, 827
HphI GGTGA 2 cut(s) 16, 251
Hpy166II GTNNAC 3 cut(s) 154, 220, 862
Hpy188I TCNGA 7 cut(s) 18, 684, 762, 798, 826, 832, 939
Hpy188III TCNNGA 4 cut(s) 322, 500, 599, 700
Hpy8I GTNNAC 3 cut(s) 154, 220, 862
HpyAV CCTTC 3 cut(s) 340, 875, 944
HpyCH4III ACNGT 2 cut(s) 168, 518
HpyCH4IV ACGT 1 cut(s) 642
HpyCH4V TGCA 3 cut(s) 95, 184, 734
HpyF3I CTNAG 1 cut(s) 840
HpySE526I ACGT 1 cut(s) 642
Hsp92II CATG 5 cut(s) 325, 544, 550, 602, 778
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 4 cut(s) 259, 370, 622, 930
LmnI GCTCC 2 cut(s) 781, 901
LpnPI CCDG 5 cut(s) 31, 221, 248, 388, 572
Lsp1109I GCAGC 4 cut(s) 55, 196, 805, 916
LweI GCATC 1 cut(s) 104
MaeI CTAG 2 cut(s) 467, 807
MaeII ACGT 1 cut(s) 642
MaeIII GTNAC 3 cut(s) 375, 643, 802
MalI GATC 4 cut(s) 261, 372, 624, 932
MboI GATC 4 cut(s) 259, 370, 622, 930
MboII GAAGA 6 cut(s) 295, 337, 425, 452, 482, 520
MfeI CAATTG 2 cut(s) 311, 353
MflI RGATCY 1 cut(s) 930
MhlI GDGCHC 2 cut(s) 16, 742
MluCI AATT 9 cut(s) 25, 192, 199, 311, 336, 353, 594, 673, 915
MmeI TCCRAC 1 cut(s) 879
MnlI CCTC 9 cut(s) 3, 127, 151, 310, 504, 655, 688, 711, 772
MseI TTAA 5 cut(s) 74, 120, 585, 627, 918
MslI CAYNNNNRTG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 236
MunI CAATTG 2 cut(s) 311, 353
MvaI CCWGG 1 cut(s) 236
NcoI CCATGG 1 cut(s) 774
NdeI CATATG 1 cut(s) 224
NdeII GATC 4 cut(s) 259, 370, 622, 930
NlaIII CATG 5 cut(s) 325, 544, 550, 602, 778
NlaIV GGNNCC 1 cut(s) 515
NspI RCATGY 1 cut(s) 550
PaeI GCATGC 1 cut(s) 550
PagI TCATGA 2 cut(s) 321, 598
PfeI GAWTC 3 cut(s) 19, 757, 827
PkrI GCNGC 5 cut(s) 70, 107, 186, 820, 906
PpuMI RGGWCCY 1 cut(s) 405
Psp5II RGGWCCY 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 234
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 515
PspPI GGNCC 3 cut(s) 349, 405, 535
PspPPI RGGWCCY 1 cut(s) 405
PstI CTGCAG 1 cut(s) 186
PstNI CAGNNNCTG 1 cut(s) 830
PsuI RGATCY 1 cut(s) 930
RsaI GTAC 1 cut(s) 425
RsaNI GTAC 1 cut(s) 424
RseI CAYNNNNRTG 1 cut(s) 545
SaqAI TTAA 5 cut(s) 74, 120, 585, 627, 918
SatI GCNGC 5 cut(s) 69, 106, 185, 819, 905
Sau3AI GATC 4 cut(s) 259, 370, 622, 930
Sau96I GGNCC 3 cut(s) 349, 405, 535
ScrFI CCNGG 1 cut(s) 236
SduI GDGCHC 2 cut(s) 16, 742
SfaNI GCATC 1 cut(s) 104
SfcI CTRYAG 1 cut(s) 182
SinI GGWCC 3 cut(s) 349, 405, 535
SmiMI CAYNNNNRTG 1 cut(s) 545
SmlI CTYRAG 1 cut(s) 908
SmoI CTYRAG 1 cut(s) 908
SpeI ACTAGT 1 cut(s) 806
SphI GCATGC 1 cut(s) 550
Sse9I AATT 9 cut(s) 25, 192, 199, 311, 336, 353, 594, 673, 915
SsiI CCGC 2 cut(s) 105, 331
SspI AATATT 1 cut(s) 116
SspMI CTAG 2 cut(s) 467, 807
StyD4I CCNGG 1 cut(s) 234
StyI CCWWGG 2 cut(s) 408, 774
TaaI ACNGT 2 cut(s) 168, 518
TaiI ACGT 1 cut(s) 645
TaqI TCGA 2 cut(s) 389, 660
TaqII GACCGA 1 cut(s) 461
TasI AATT 9 cut(s) 25, 192, 199, 311, 336, 353, 594, 673, 915
TatI WGTACW 1 cut(s) 423
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 3 cut(s) 19, 757, 827
Tru1I TTAA 5 cut(s) 74, 120, 585, 627, 918
Tru9I TTAA 5 cut(s) 74, 120, 585, 627, 918
TscAI CASTG 4 cut(s) 131, 173, 277, 844
TseI GCWGC 4 cut(s) 68, 184, 818, 904
TspDTI ATGAA 3 cut(s) 338, 587, 615
TspRI CASTG 4 cut(s) 131, 173, 277, 844
VpaK11BI GGWCC 3 cut(s) 349, 405, 535
XagI CCTNNNNNAGG 1 cut(s) 460
XceI RCATGY 1 cut(s) 550
XmiI GTMKAC 2 cut(s) 219, 861
XspI CTAG 2 cut(s) 467, 807
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.