Rh1BG204000

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000446
Physical Location & Seq
Forward (+)
32235824 .. 32243729
7906 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG204000.1

Sequence Viewer

Length: 1095 bp
ATGAGGGAAGACAGTGCTGAAGAACTGCAGTTTTATAGTTACTGGTATTATGCAGAAGTTGTGATTCCAACCAGGCAGGAAGCTAAAATGGCCAGATTGCAATTCCGTGATGCCAATGAGGATGATGTACCATCCGAGGAACTCCCACATCTCCTACTCATTCATCCACCATCATCCTTCACACGTCTCCCATCTGAAATCTGTGAAAAGTTCCGTGTCCTGAAAGCATGGGAGTCGCCACTCCCTCTAGACCAGTTCCTGATCACCCATGCATGCTCAATCCAAGCCCTACTCATTCGCGGTTTTCTTATGAATAACACAGACATCCTTCAGCAGCTACCGGCACTGAAACTCATCATAACAATCAGCGTTGGTGTGAACCACATTGACATGGCAGAGTGCCGGCAGCGTGGGATTTCTGTGACCAATGCGGGAAGTGTATACTCGGAAGATGTTGCTGATATTGCTGTTGGGTTGTACATTGATGTGCTTAGAAAACTTTCAGCAGCAGACCGGTATGTAAGACAAGGGCTTTGGAGTAGCAAAGGAAATTACCCTCTTGGTTCCAAGTTAGGTGGGAAGCGAGTTGGGATTGTTGGATTAGGAAGCATTGGCTCAGAAGTTGCTAAACGACTTGAGGCCTTTGGCTGCATCATCTCATACAATTCAAGGAGGGAAAAGCCAACTTCGCCATACCCCTTCTATTCTATCATATCTGAACTTGCAGCCAATAGTGATGCCCTCATCATCTGTTGTGCATTAACAGAACAAACCCACCATATGATTAACAAGGAAGTCTTGGCTTCACTGGGAAGAGAGGGTGTGATTGTTAATGTAGGCCGCGGGGCAATAATTGATGAGAAAGAACTGGTTAAGTGTTTGGTGAGTGGGGAAATAAGAGGTGCTGGTTTTGATGTGTTTGAGAATGAGCCTCAAGTTCCGGAAGAGCTCTTTACATTGGACAATGTTGTTCTATCCCCACATACGGCTATTTTTACTCCGGAATCTTTGCAGAGTGTGAAAGAAGTACTAATCGCCAATGTGGAAGCTTTCTTCTTGAATAAACCTCTGGTTACTCCTGTCATGGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

364

Amino Acids

40.41

Weight (kDa)

5.13

Isoelectric Point (pI)

48.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 87 - 361 7.5e-19 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 158 - 330 8.7e-52 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 441
AccII CGCG 2 cut(s) 300, 843
AccIII TCCGGA 2 cut(s) 940, 1000
AciI CCGC 4 cut(s) 300, 431, 841, 843
AcoI YGGCCR 1 cut(s) 90
AcuI CTGAAG 2 cut(s) 39, 314
AfaI GTAC 3 cut(s) 129, 479, 1029
AfiI CCNNNNNNNGG 1 cut(s) 985
AflIII ACRYGT 1 cut(s) 182
AgeI ACCGGT 1 cut(s) 513
AgsI TTSAA 2 cut(s) 669, 1060
AjiI CACGTC 1 cut(s) 185
AjnI CCWGG 1 cut(s) 71
AluBI AGCT 4 cut(s) 83, 337, 949, 1049
AluI AGCT 4 cut(s) 83, 337, 949, 1049
Alw21I GWGCWC 1 cut(s) 951
Alw26I GTCTC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 259
Aor13HI TCCGGA 2 cut(s) 940, 1000
AoxI GGCC 3 cut(s) 90, 639, 838
ApeKI GCWGC 5 cut(s) 334, 406, 506, 648, 725
ArsI GACNNNNNNTTYG 2 cut(s) 516, 548
AsiGI ACCGGT 1 cut(s) 513
Asp700I GAANNNNTTC 1 cut(s) 499
AsuHPI GGTGA 2 cut(s) 256, 895
BalI TGGCCA 1 cut(s) 92
BanII GRGCYC 1 cut(s) 951
BbsI GAAGAC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 951
BbvI GCAGC 5 cut(s) 346, 418, 518, 635, 737
BccI CCATC 3 cut(s) 139, 178, 199
BceAI ACGGC 1 cut(s) 1002
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 1 cut(s) 261
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 248
BfmI CTRYAG 1 cut(s) 26
BisI GCNGC 6 cut(s) 335, 407, 507, 649, 726, 841
BlsI GCNGC 6 cut(s) 336, 408, 508, 650, 727, 842
BmcAI AGTACT 1 cut(s) 1029
Bme1390I CCNGG 1 cut(s) 73
BmgBI CACGTC 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 1 cut(s) 73
BmrI ACTGGG 1 cut(s) 818
BmsI GCATC 3 cut(s) 100, 660, 727
BmuI ACTGGG 1 cut(s) 818
BpiI GAAGAC 1 cut(s) 15
BpuEI CTTGAG 2 cut(s) 656, 918
BsaJI CCNNGG 2 cut(s) 135, 841
BsaWI WCCGGW 3 cut(s) 513, 940, 1000
Bsc4I CCNNNNNNNGG 1 cut(s) 985
Bse118I RCCGGY 3 cut(s) 340, 402, 513
Bse1I ACTGG 4 cut(s) 47, 253, 813, 873
BseAI TCCGGA 2 cut(s) 940, 1000
BseBI CCWGG 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 135, 841
BseGI GGATG 6 cut(s) 127, 131, 163, 173, 324, 1093
BseLI CCNNNNNNNGG 1 cut(s) 985
BseMII CTCAG 1 cut(s) 630
BseNI ACTGG 4 cut(s) 47, 253, 813, 873
BseXI GCAGC 5 cut(s) 346, 418, 518, 635, 737
Bsh1236I CGCG 2 cut(s) 300, 843
BshFI GGCC 3 cut(s) 92, 641, 840
BshTI ACCGGT 1 cut(s) 513
BsiHKAI GWGCWC 1 cut(s) 951
BsiSI CCGG 5 cut(s) 341, 403, 514, 941, 1001
BslI CCNNNNNNNGG 1 cut(s) 985
BsmAI GTCTC 1 cut(s) 191
BsmBI CGTCTC 1 cut(s) 191
BsnI GGCC 3 cut(s) 92, 641, 840
Bsp1286I GDGCHC 1 cut(s) 951
Bsp13I TCCGGA 2 cut(s) 940, 1000
Bsp1407I TGTACA 1 cut(s) 477
Bsp143I GATC 1 cut(s) 261
BspACI CCGC 4 cut(s) 300, 431, 841, 843
BspANI GGCC 3 cut(s) 92, 641, 840
BspCNI CTCAG 1 cut(s) 629
BspEI TCCGGA 2 cut(s) 940, 1000
BspFNI CGCG 2 cut(s) 300, 843
BspLI GGNNCC 1 cut(s) 565
BspMAI CTGCAG 1 cut(s) 30
BspQI GCTCTTC 1 cut(s) 939
BsrFI RCCGGY 3 cut(s) 340, 402, 513
BsrGI TGTACA 1 cut(s) 477
BsrI ACTGG 4 cut(s) 47, 253, 813, 873
BssAI RCCGGY 3 cut(s) 340, 402, 513
BssECI CCNNGG 2 cut(s) 135, 841
BssMI GATC 1 cut(s) 261
BssNAI GTATAC 1 cut(s) 442
Bst1107I GTATAC 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 2 cut(s) 808, 939
BstAUI TGTACA 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 274, 404
BstDEI CTNAG 2 cut(s) 491, 616
BstDSI CCRYGG 1 cut(s) 841
BstF5I GGATG 6 cut(s) 127, 131, 163, 173, 324, 1093
BstFNI CGCG 2 cut(s) 300, 843
BstKTI GATC 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 261
BstMWI GCNNNNNNNGC 3 cut(s) 89, 464, 688
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 1 cut(s) 276
BstSCI CCNGG 1 cut(s) 71
BstSFI CTRYAG 1 cut(s) 26
BstUI CGCG 2 cut(s) 300, 843
BstV1I GCAGC 5 cut(s) 346, 418, 518, 635, 737
BstV2I GAAGAC 1 cut(s) 15
BstZ17I GTATAC 1 cut(s) 442
BsuRI GGCC 3 cut(s) 92, 641, 840
BtgI CCRYGG 1 cut(s) 841
BtrI CACGTC 1 cut(s) 185
BtsCI GGATG 6 cut(s) 127, 131, 163, 173, 324, 1093
BtsIMutI CAGTG 3 cut(s) 19, 344, 806
Cac8I GCNNGC 2 cut(s) 274, 404
CaiI CAGNNNCTG 1 cut(s) 259
Cfr10I RCCGGY 3 cut(s) 340, 402, 513
Cfr42I CCGCGG 1 cut(s) 844
Csp6I GTAC 3 cut(s) 128, 478, 1028
CspAI ACCGGT 1 cut(s) 513
CspCI CAANNNNNGTGG 2 cut(s) 556, 591
CviAII CATG 5 cut(s) 228, 269, 273, 391, 1084
CviQI GTAC 3 cut(s) 128, 478, 1028
DdeI CTNAG 2 cut(s) 491, 616
DpnI GATC 1 cut(s) 263
DpnII GATC 1 cut(s) 261
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 808, 939
EarI CTCTTC 2 cut(s) 808, 939
Ecl136II GAGCTC 1 cut(s) 949
Eco147I AGGCCT 1 cut(s) 641
Eco24I GRGCYC 1 cut(s) 951
Eco53kI GAGCTC 1 cut(s) 949
Eco57I CTGAAG 2 cut(s) 39, 314
EcoICRI GAGCTC 1 cut(s) 949
EcoRII CCWGG 1 cut(s) 71
EcoT22I ATGCAT 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 951
Esp3I CGTCTC 1 cut(s) 191
FaeI CATG 5 cut(s) 231, 272, 276, 394, 1087
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FatI CATG 5 cut(s) 227, 268, 272, 390, 1083
FauI CCCGC 2 cut(s) 424, 836
FauNDI CATATG 1 cut(s) 780
FbaI TGATCA 1 cut(s) 261
FblI GTMKAC 1 cut(s) 441
Fnu4HI GCNGC 6 cut(s) 335, 407, 507, 649, 726, 841
FokI GGATG 5 cut(s) 118, 134, 150, 160, 311
FriOI GRGCYC 1 cut(s) 951
Fsp4HI GCNGC 6 cut(s) 335, 407, 507, 649, 726, 841
FspBI CTAG 1 cut(s) 248
GluI GCNGC 6 cut(s) 335, 407, 507, 649, 726, 841
HaeIII GGCC 3 cut(s) 92, 641, 840
HapII CCGG 5 cut(s) 341, 403, 514, 941, 1001
Hin1II CATG 5 cut(s) 231, 272, 276, 394, 1087
HindIII AAGCTT 1 cut(s) 1047
HinfI GANTC 3 cut(s) 64, 233, 1004
HpaII CCGG 5 cut(s) 341, 403, 514, 941, 1001
HphI GGTGA 2 cut(s) 256, 895
Hpy166II GTNNAC 2 cut(s) 379, 442
Hpy188I TCNGA 5 cut(s) 136, 196, 448, 619, 718
Hpy188III TCNNGA 6 cut(s) 220, 248, 259, 941, 1001, 1057
Hpy8I GTNNAC 2 cut(s) 379, 442
HpyAV CCTTC 3 cut(s) 187, 338, 709
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 8 cut(s) 28, 53, 100, 272, 651, 725, 758, 1012
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 464, 688
HpyF3I CTNAG 2 cut(s) 491, 616
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 5 cut(s) 231, 272, 276, 394, 1087
Kpn2I TCCGGA 2 cut(s) 940, 1000
KroI GCCGGC 1 cut(s) 402
KroNI GCCGGC 1 cut(s) 404
Ksp22I TGATCA 1 cut(s) 261
KspI CCGCGG 1 cut(s) 844
Kzo9I GATC 1 cut(s) 261
LguI GCTCTTC 1 cut(s) 939
Lsp1109I GCAGC 5 cut(s) 346, 418, 518, 635, 737
LweI GCATC 3 cut(s) 100, 660, 727
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 184
MaeIII GTNAC 3 cut(s) 38, 421, 1072
MalI GATC 1 cut(s) 263
MboI GATC 1 cut(s) 261
MboII GAAGA 6 cut(s) 20, 32, 461, 825, 956, 1045
MhlI GDGCHC 1 cut(s) 951
MlsI TGGCCA 1 cut(s) 92
MluCI AATT 4 cut(s) 101, 550, 664, 852
MluNI TGGCCA 1 cut(s) 92
MlyI GAGTC 1 cut(s) 242
MmeI TCCRAC 2 cut(s) 92, 577
Mox20I TGGCCA 1 cut(s) 92
Mph1103I ATGCAT 1 cut(s) 274
MroI TCCGGA 2 cut(s) 940, 1000
MroNI GCCGGC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 499
MscI TGGCCA 1 cut(s) 92
MseI TTAA 4 cut(s) 761, 786, 831, 873
MslI CAYNNNNRTG 2 cut(s) 389, 485
Msp20I TGGCCA 1 cut(s) 92
MspA1I CMGCKG 1 cut(s) 843
MspI CCGG 5 cut(s) 341, 403, 514, 941, 1001
MspR9I CCNGG 1 cut(s) 73
MvaI CCWGG 1 cut(s) 73
MvnI CGCG 2 cut(s) 300, 843
MwoI GCNNNNNNNGC 3 cut(s) 89, 464, 688
NaeI GCCGGC 1 cut(s) 404
NdeI CATATG 1 cut(s) 780
NdeII GATC 1 cut(s) 261
NgoMIV GCCGGC 1 cut(s) 402
NlaIII CATG 5 cut(s) 231, 272, 276, 394, 1087
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 421
NsiI ATGCAT 1 cut(s) 274
NspI RCATGY 1 cut(s) 276
PaeI GCATGC 1 cut(s) 276
PceI AGGCCT 1 cut(s) 641
PciSI GCTCTTC 1 cut(s) 939
PdiI GCCGGC 1 cut(s) 404
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 2 cut(s) 64, 1004
PinAI ACCGGT 1 cut(s) 513
PkrI GCNGC 6 cut(s) 336, 408, 508, 650, 727, 842
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 951
Psp6I CCWGG 1 cut(s) 71
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 1 cut(s) 565
PstI CTGCAG 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 259
RsaI GTAC 3 cut(s) 129, 479, 1029
RsaNI GTAC 3 cut(s) 128, 478, 1028
RseI CAYNNNNRTG 2 cut(s) 389, 485
SacI GAGCTC 1 cut(s) 951
SacII CCGCGG 1 cut(s) 844
SapI GCTCTTC 1 cut(s) 939
SaqAI TTAA 4 cut(s) 761, 786, 831, 873
SatI GCNGC 6 cut(s) 335, 407, 507, 649, 726, 841
Sau3AI GATC 1 cut(s) 261
ScaI AGTACT 1 cut(s) 1029
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 73
SduI GDGCHC 1 cut(s) 951
SetI ASST 8 cut(s) 85, 187, 339, 577, 904, 951, 1051, 1069
SfaNI GCATC 3 cut(s) 100, 660, 727
SfcI CTRYAG 1 cut(s) 26
Sfr303I CCGCGG 1 cut(s) 844
SgrBI CCGCGG 1 cut(s) 844
SmiMI CAYNNNNRTG 2 cut(s) 389, 485
SmlI CTYRAG 2 cut(s) 635, 933
SmoI CTYRAG 2 cut(s) 635, 933
SphI GCATGC 1 cut(s) 276
Sse9I AATT 4 cut(s) 101, 550, 664, 852
SseBI AGGCCT 1 cut(s) 641
SsiI CCGC 4 cut(s) 300, 431, 841, 843
SspMI CTAG 1 cut(s) 248
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 641
StyD4I CCNGG 1 cut(s) 71
TaaI ACNGT 1 cut(s) 14
TaiI ACGT 1 cut(s) 187
TasI AATT 4 cut(s) 101, 550, 664, 852
TatI WGTACW 2 cut(s) 477, 1027
TauI GCSGC 1 cut(s) 843
TfiI GAWTC 2 cut(s) 64, 1004
Tru1I TTAA 4 cut(s) 761, 786, 831, 873
Tru9I TTAA 4 cut(s) 761, 786, 831, 873
TscAI CASTG 3 cut(s) 19, 351, 813
TseFI GTSAC 1 cut(s) 421
TseI GCWGC 5 cut(s) 334, 406, 506, 648, 725
Tsp45I GTSAC 1 cut(s) 421
TspDTI ATGAA 2 cut(s) 152, 326
TspGWI ACGGA 2 cut(s) 95, 203
TspRI CASTG 3 cut(s) 19, 351, 813
XbaI TCTAGA 1 cut(s) 247
XceI RCATGY 1 cut(s) 276
XmiI GTMKAC 1 cut(s) 441
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 1 cut(s) 248
ZrmI AGTACT 1 cut(s) 1029
Zsp2I ATGCAT 1 cut(s) 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.