Rorug01G0218800

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000446
Physical Location & Seq
Reverse (-)
31282436 .. 31283812
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0218800.1

Sequence Viewer

Length: 732 bp
ATGGCCTCCTCCTCGTCTGTGTCACCCAATAAGTGGACTTCCCAGACCCCCAACCCTGACATATCGGGGTTGAAATGGACCAAGAAAAACTCACGACACTCTCGCCAGTCGGGCAATGAACCCCAACTCCCCAACCATGACTCGGCAGCTGAGCTACCTGACCACAACTCCGACACTGGGGAAAACGTCATTGACCAGAACGAAGTCTTGTCCGAACAGCCGCACCCCAAGAAACCAAATTTGAAGGTTTACATCAGGCTCAAGCTGGACAAAGACGACGCTCCTCAGGTGAAAAAAAAAGCAGAGGATCAAAACCCTAAATCTGATGATCCTGCTGTTGCTGCTTTGGCTGCTTTAGCTGCGGGGGAAGGATTGGAGGAGAAGGGGAACAAAACATGGAATCTGAGGCCCAGGAAACCGGTGAAGAAGCCTAATGGGGAGGCTAGTGCCATGAAGACCGGTGCACCGGTGGGGCAGCATAACGAAATCCCGGAGCTGGGTCGGTCCAGACTGGGTAGAGGTTGTCAAACGAAAAAACCTAAGGAGCCGAGGATGGAGATATCGCTTACGTTGACCAAAGAGGAGATTGAGGAGGATCTTTTACTCTTGACAGGGAAGAAGCCGTCGCGCCGGCCCAAGAGGAGGCCTAGGAGTCTGCAGAAGCAGCTGGATAATATAACTCCAGGGATGTATCTGGATATGCTGTCAGCGGAAGCCTACCAGGTTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

243

Amino Acids

26.97

Weight (kDa)

9.33

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1639 PF07797 191 - 240 3.3e-20 Protein of unknown function (DUF1639)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 33
AccII CGCG 1 cut(s) 628
AciI CCGC 3 cut(s) 221, 362, 710
AclWI GGATC 3 cut(s) 315, 323, 603
AcsI RAATTY 1 cut(s) 238
AfiI CCNNNNNNNGG 6 cut(s) 33, 142, 177, 496, 497, 642
AgeI ACCGGT 3 cut(s) 418, 458, 466
AgsI TTSAA 2 cut(s) 73, 244
AjnI CCWGG 3 cut(s) 410, 682, 720
AloI GAACNNNNNNTCC 2 cut(s) 111, 143
AluBI AGCT 6 cut(s) 149, 154, 265, 359, 496, 667
AluI AGCT 6 cut(s) 149, 154, 265, 359, 496, 667
Alw21I GWGCWC 1 cut(s) 466
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 3 cut(s) 315, 323, 603
AoxI GGCC 4 cut(s) 3, 407, 632, 644
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 6 cut(s) 146, 341, 350, 359, 475, 664
ApoI RAATTY 1 cut(s) 238
AsiGI ACCGGT 3 cut(s) 418, 458, 466
AspA2I CCTAGG 1 cut(s) 647
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 78, 408, 504, 633
AsuC2I CCSGG 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 15, 301, 433
AvaII GGWCC 2 cut(s) 78, 504
AvrII CCTAGG 1 cut(s) 647
AxyI CCTNAGG 2 cut(s) 285, 540
BaeGI GKGCMC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 6 cut(s) 158, 328, 337, 346, 487, 676
BccI CCATC 1 cut(s) 547
BceAI ACGGC 1 cut(s) 607
BciT130I CCWGG 3 cut(s) 412, 684, 722
BcnI CCSGG 1 cut(s) 491
BfaI CTAG 2 cut(s) 444, 648
BfmI CTRYAG 1 cut(s) 656
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 7 cut(s) 147, 221, 342, 351, 360, 476, 665
BlnI CCTAGG 1 cut(s) 647
BlpI GCTNAGC 1 cut(s) 150
BlsI GCNGC 7 cut(s) 148, 222, 343, 352, 361, 477, 666
Bme1390I CCNGG 4 cut(s) 412, 491, 684, 722
Bme18I GGWCC 2 cut(s) 78, 504
BmgT120I GGNCC 4 cut(s) 78, 408, 504, 633
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 4 cut(s) 412, 491, 684, 722
BmrI ACTGGG 2 cut(s) 186, 521
BmuI ACTGGG 2 cut(s) 186, 521
BpiI GAAGAC 1 cut(s) 461
BpmI CTGGAG 1 cut(s) 666
Bpu1102I GCTNAGC 1 cut(s) 150
BpuEI CTTGAG 1 cut(s) 245
BpuMI CCSGG 1 cut(s) 491
BsaJI CCNNGG 4 cut(s) 410, 548, 647, 683
BsaWI WCCGGW 3 cut(s) 418, 458, 466
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 152, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 33, 142, 177, 496, 497, 642
Bse118I RCCGGY 4 cut(s) 418, 458, 466, 630
Bse1I ACTGG 3 cut(s) 106, 181, 516
Bse21I CCTNAGG 2 cut(s) 285, 540
Bse3DI GCAATG 1 cut(s) 121
BseBI CCWGG 3 cut(s) 412, 684, 722
BseDI CCNNGG 4 cut(s) 410, 548, 647, 683
BseGI GGATG 2 cut(s) 558, 693
BseLI CCNNNNNNNGG 6 cut(s) 33, 142, 177, 496, 497, 642
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 3 cut(s) 141, 299, 395
BseNI ACTGG 3 cut(s) 106, 181, 516
BseRI GAGGAG 5 cut(s) 273, 392, 596, 605, 655
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 6 cut(s) 158, 328, 337, 346, 487, 676
BseYI CCCAGC 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 628
BshFI GGCC 4 cut(s) 5, 409, 634, 646
BshTI ACCGGT 3 cut(s) 418, 458, 466
BsiHKAI GWGCWC 1 cut(s) 466
BsiSI CCGG 5 cut(s) 419, 459, 467, 491, 631
BslI CCNNNNNNNGG 6 cut(s) 33, 142, 177, 496, 497, 642
BsnI GGCC 4 cut(s) 5, 409, 634, 646
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 3 cut(s) 307, 328, 595
Bsp1720I GCTNAGC 1 cut(s) 150
BspACI CCGC 3 cut(s) 221, 362, 710
BspANI GGCC 4 cut(s) 5, 409, 634, 646
BspCNI CTCAG 3 cut(s) 142, 298, 396
BspFNI CGCG 1 cut(s) 628
BspLI GGNNCC 1 cut(s) 546
BspMAI CTGCAG 1 cut(s) 660
BspPI GGATC 3 cut(s) 315, 323, 603
BsrDI GCAATG 1 cut(s) 121
BsrFI RCCGGY 4 cut(s) 418, 458, 466, 630
BsrI ACTGG 3 cut(s) 106, 181, 516
BssAI RCCGGY 4 cut(s) 418, 458, 466, 630
BssECI CCNNGG 4 cut(s) 410, 548, 647, 683
BssMI GATC 3 cut(s) 307, 328, 595
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 3 cut(s) 412, 684, 722
BstC8I GCNNGC 1 cut(s) 632
BstDEI CTNAG 4 cut(s) 150, 285, 404, 540
BstF5I GGATG 2 cut(s) 558, 693
BstFNI CGCG 1 cut(s) 628
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 3 cut(s) 310, 331, 598
BstMBI GATC 3 cut(s) 307, 328, 595
BstMWI GCNNNNNNNGC 7 cut(s) 111, 341, 347, 350, 356, 359, 664
BstNI CCWGG 3 cut(s) 412, 684, 722
BstSCI CCNGG 4 cut(s) 410, 489, 682, 720
BstSFI CTRYAG 1 cut(s) 656
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 628
BstV1I GCAGC 6 cut(s) 158, 328, 337, 346, 487, 676
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu36I CCTNAGG 2 cut(s) 285, 540
BsuRI GGCC 4 cut(s) 5, 409, 634, 646
BtsCI GGATG 2 cut(s) 558, 693
BtsIMutI CAGTG 1 cut(s) 174
Cac8I GCNNGC 1 cut(s) 632
CfoI GCGC 1 cut(s) 630
Cfr10I RCCGGY 4 cut(s) 418, 458, 466, 630
Cfr13I GGNCC 4 cut(s) 78, 408, 504, 633
CseI GACGC 1 cut(s) 287
CsiI ACCWGGT 1 cut(s) 720
CspAI ACCGGT 3 cut(s) 418, 458, 466
CviAII CATG 3 cut(s) 137, 396, 451
DdeI CTNAG 4 cut(s) 150, 285, 404, 540
DpnI GATC 3 cut(s) 309, 330, 597
DpnII GATC 3 cut(s) 307, 328, 595
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 646
Eco32I GATATC 1 cut(s) 561
Eco47I GGWCC 2 cut(s) 78, 504
Eco81I CCTNAGG 2 cut(s) 285, 540
EcoRII CCWGG 3 cut(s) 410, 682, 720
EcoRV GATATC 1 cut(s) 561
EcoT14I CCWWGG 1 cut(s) 647
ErhI CCWWGG 1 cut(s) 647
FaeI CATG 3 cut(s) 140, 399, 454
FaiI YATR 7 cut(s) 62, 138, 397, 452, 480, 677, 701
FatI CATG 3 cut(s) 136, 395, 450
FauI CCCGC 1 cut(s) 355
Fnu4HI GCNGC 7 cut(s) 147, 221, 342, 351, 360, 476, 665
FokI GGATG 2 cut(s) 565, 700
Fsp4HI GCNGC 7 cut(s) 147, 221, 342, 351, 360, 476, 665
FspBI CTAG 2 cut(s) 444, 648
GlaI GCGC 1 cut(s) 629
GluI GCNGC 7 cut(s) 147, 221, 342, 351, 360, 476, 665
GsaI CCCAGC 1 cut(s) 500
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 4 cut(s) 5, 409, 634, 646
HapII CCGG 5 cut(s) 419, 459, 467, 491, 631
HgaI GACGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 630
Hin1II CATG 3 cut(s) 140, 399, 454
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HincII GTYRAC 1 cut(s) 573
HindII GTYRAC 1 cut(s) 573
HinfI GANTC 3 cut(s) 140, 400, 652
HpaII CCGG 5 cut(s) 419, 459, 467, 491, 631
HphI GGTGA 3 cut(s) 15, 301, 433
Hpy166II GTNNAC 4 cut(s) 36, 250, 464, 573
Hpy188I TCNGA 4 cut(s) 172, 214, 325, 405
Hpy188III TCNNGA 4 cut(s) 93, 507, 607, 695
Hpy8I GTNNAC 4 cut(s) 36, 250, 464, 573
Hpy99I CGWCG 2 cut(s) 281, 628
HpyAV CCTTC 3 cut(s) 238, 362, 376
HpyCH4IV ACGT 2 cut(s) 186, 569
HpyCH4V TGCA 2 cut(s) 464, 658
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 341, 347, 350, 356, 359, 664
HpyF3I CTNAG 4 cut(s) 150, 285, 404, 540
HpySE526I ACGT 2 cut(s) 186, 569
Hsp92II CATG 3 cut(s) 140, 399, 454
HspAI GCGC 1 cut(s) 628
KroI GCCGGC 1 cut(s) 630
KroNI GCCGGC 1 cut(s) 632
Kzo9I GATC 3 cut(s) 307, 328, 595
LmnI GCTCC 3 cut(s) 286, 493, 544
Lsp1109I GCAGC 6 cut(s) 158, 328, 337, 346, 487, 676
MabI ACCWGGT 1 cut(s) 720
MaeI CTAG 2 cut(s) 444, 648
MaeII ACGT 2 cut(s) 186, 569
MaeIII GTNAC 1 cut(s) 21
MalI GATC 3 cut(s) 309, 330, 597
MboI GATC 3 cut(s) 307, 328, 595
MboII GAAGA 3 cut(s) 436, 466, 628
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 1 cut(s) 238
MlyI GAGTC 2 cut(s) 134, 661
MmeI TCCRAC 1 cut(s) 195
MroNI GCCGGC 1 cut(s) 630
MseI TTAA 1 cut(s) 730
MspA1I CMGCKG 3 cut(s) 149, 667, 710
MspI CCGG 5 cut(s) 419, 459, 467, 491, 631
MspR9I CCNGG 4 cut(s) 412, 491, 684, 722
MvaI CCWGG 3 cut(s) 412, 684, 722
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 7 cut(s) 111, 341, 347, 350, 356, 359, 664
NaeI GCCGGC 1 cut(s) 632
NciI CCSGG 1 cut(s) 491
NdeII GATC 3 cut(s) 307, 328, 595
NgoMIV GCCGGC 1 cut(s) 630
NlaIII CATG 3 cut(s) 140, 399, 454
NlaIV GGNNCC 1 cut(s) 546
NmeAIII GCCGAG 2 cut(s) 122, 573
NmuCI GTSAC 1 cut(s) 21
PceI AGGCCT 1 cut(s) 646
PdiI GCCGGC 1 cut(s) 632
PfeI GAWTC 1 cut(s) 400
PflMI CCANNNNNTGG 1 cut(s) 33
PfoI TCCNGGA 1 cut(s) 489
PinAI ACCGGT 3 cut(s) 418, 458, 466
PkrI GCNGC 7 cut(s) 148, 222, 343, 352, 361, 477, 666
PleI GAGTC 2 cut(s) 134, 660
PpsI GAGTC 2 cut(s) 134, 660
Psp6I CCWGG 3 cut(s) 410, 682, 720
PspFI CCCAGC 1 cut(s) 496
PspGI CCWGG 3 cut(s) 410, 682, 720
PspN4I GGNNCC 1 cut(s) 546
PspPI GGNCC 4 cut(s) 78, 408, 504, 633
PstI CTGCAG 1 cut(s) 660
PsuI RGATCY 1 cut(s) 595
PvuII CAGCTG 2 cut(s) 149, 667
SaqAI TTAA 1 cut(s) 730
SatI GCNGC 7 cut(s) 147, 221, 342, 351, 360, 476, 665
Sau3AI GATC 3 cut(s) 307, 328, 595
Sau96I GGNCC 4 cut(s) 78, 408, 504, 633
SchI GAGTC 2 cut(s) 134, 661
ScrFI CCNGG 4 cut(s) 412, 491, 684, 722
SduI GDGCHC 1 cut(s) 466
SexAI ACCWGGT 1 cut(s) 720
SfcI CTRYAG 1 cut(s) 656
SgrAI CRCCGGYG 1 cut(s) 466
SinI GGWCC 2 cut(s) 78, 504
SmlI CTYRAG 1 cut(s) 260
SmoI CTYRAG 1 cut(s) 260
Sse9I AATT 1 cut(s) 238
SseBI AGGCCT 1 cut(s) 646
SsiI CCGC 3 cut(s) 221, 362, 710
SspMI CTAG 2 cut(s) 444, 648
StuI AGGCCT 1 cut(s) 646
StyD4I CCNGG 4 cut(s) 410, 489, 682, 720
StyI CCWWGG 1 cut(s) 647
TaiI ACGT 2 cut(s) 189, 572
TaqII GACCGA 1 cut(s) 492
TasI AATT 1 cut(s) 238
TauI GCSGC 1 cut(s) 223
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 730
Tru9I TTAA 1 cut(s) 730
TscAI CASTG 1 cut(s) 181
TseFI GTSAC 1 cut(s) 21
TseI GCWGC 6 cut(s) 146, 341, 350, 359, 475, 664
Tsp45I GTSAC 1 cut(s) 21
TspDTI ATGAA 2 cut(s) 132, 467
TspRI CASTG 1 cut(s) 181
Van91I CCANNNNNTGG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 78, 504
XapI RAATTY 1 cut(s) 238
XmaJI CCTAGG 1 cut(s) 647
XspI CTAG 2 cut(s) 444, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.