Rroxscaffold_4G00303770

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000446
Physical Location & Seq
Reverse (-)
24142520 .. 24143699
1180 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303770.1

Sequence Viewer

Length: 894 bp
ATGGCAGATGGTAATCACAGTAAGCCCCCTACAGTTCTGCCTGGGACTCTCCTCTCCCCTCAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCCCTCCTCTCCTCCGTCGGTGGGCTGCCCATCACCGCCCAAATCCTCCACTTGCTCCCTTCCCTCAAATTCATAGCCACCACCAGCGCCGGCATTGACCACGTTGACTCTGCCGAGTGTCGACGCCGCGGCGTTGCTGTCGCCAACGCCGCGAAAGCCTTTGCTGAGGACGCTGCTGACATGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGCTGTCGGCTTCGGATCGGTATGTTGGGGACGGGCTCTGGGCTACCAGAGGAAACTATCCTCTTGGAAAGAAGGTTGGTATTGTTGGATTGGGGAACATTGGTTTAGAAGTTGCAAAAAGATTGGAGGCATTTGGCTGCAGTGTCTTGTACAATTCAAGGAGCGAAAAGCCATCTTTGCCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATTATCTGTTGTGCATTGACAGAGCAAACCCACCACATGATCAACAGGGAAGTTTTCTCCGCGTTGGGAAGAGAGGGGGTTATCGTTAATGTAGGGCGCGGAGCTATTATCAATGAGAAAGAAATGGTTGAGTATTTGGTGCGAGGAGAGATCGGAGGTGCTGGCCTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTATGCATTGGATAATGTTGTACTGTCACCGCATCAAGCTGTCGCTACACATGAAGCTATCGTGGCTTTGGGTGAAGTAGTAACAGGGGAATTTGGAAGCATTCTTCTCAAACAAACCGCTGCTTTCTCCGGCTGTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

297

Amino Acids

31.53

Weight (kDa)

5.29

Isoelectric Point (pI)

31.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 27 - 279 8.3e-17 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 97 - 264 5.7e-48 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 220
AccII CGCG 4 cut(s) 228, 251, 608, 645
AciI CCGC 9 cut(s) 88, 135, 226, 228, 249, 606, 645, 782, 870
AclWI GGATC 1 cut(s) 336
AcsI RAATTY 2 cut(s) 167, 842
AcyI GRCGYC 1 cut(s) 223
AfaI GTAC 2 cut(s) 464, 774
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 1 cut(s) 471
AjnI CCWGG 3 cut(s) 40, 96, 711
AluBI AGCT 6 cut(s) 316, 523, 650, 751, 791, 809
AluI AGCT 6 cut(s) 316, 523, 650, 751, 791, 809
Alw21I GWGCWC 1 cut(s) 753
AlwI GGATC 1 cut(s) 336
AoxI GGCC 2 cut(s) 99, 709
ApeKI GCWGC 5 cut(s) 124, 272, 450, 527, 872
ApoI RAATTY 2 cut(s) 167, 842
AspLEI GCGC 2 cut(s) 188, 645
AspS9I GGNCC 1 cut(s) 100
AsuHPI GGTGA 3 cut(s) 124, 771, 836
BanII GRGCYC 2 cut(s) 351, 753
Bbv12I GWGCWC 1 cut(s) 753
BbvCI CCTCAGC 1 cut(s) 264
BbvI GCAGC 5 cut(s) 111, 259, 437, 539, 859
BccI CCATC 3 cut(s) 2, 137, 493
BciT130I CCWGG 3 cut(s) 42, 98, 713
BclI TGATCA 1 cut(s) 585
BfmI CTRYAG 2 cut(s) 30, 451
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 8 cut(s) 125, 226, 229, 249, 273, 451, 528, 873
BlsI GCNGC 8 cut(s) 126, 227, 230, 250, 274, 452, 529, 874
Bme1390I CCNGG 3 cut(s) 42, 98, 713
BmgT120I GGNCC 1 cut(s) 100
BmrFI CCNGG 3 cut(s) 42, 98, 713
BmsI GCATC 2 cut(s) 529, 793
Bpu10I CCTNAGC 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 223
BsaJI CCNNGG 2 cut(s) 41, 226
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 67
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 3 cut(s) 42, 98, 713
BseDI CCNNGG 2 cut(s) 41, 226
BseGI GGATG 1 cut(s) 721
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 120
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 67
BseRI GAGGAG 4 cut(s) 41, 95, 100, 705
BseXI GCAGC 5 cut(s) 111, 259, 437, 539, 859
Bsh1236I CGCG 4 cut(s) 228, 251, 608, 645
BshFI GGCC 2 cut(s) 101, 711
BsiHKAI GWGCWC 1 cut(s) 753
BsiSI CCGG 2 cut(s) 189, 882
BslFI GGGAC 2 cut(s) 58, 356
BslI CCNNNNNNNGG 1 cut(s) 120
BsmFI GGGAC 2 cut(s) 58, 356
BsmI GAATGC 1 cut(s) 852
BsnI GGCC 2 cut(s) 101, 711
Bsp1286I GDGCHC 2 cut(s) 351, 753
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 3 cut(s) 328, 585, 696
BspACI CCGC 9 cut(s) 88, 135, 226, 228, 249, 606, 645, 782, 870
BspANI GGCC 2 cut(s) 101, 711
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 4 cut(s) 228, 251, 608, 645
BspHI TCATGA 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 455
BspPI GGATC 1 cut(s) 336
BsrBI CCGCTC 1 cut(s) 90
BsrFI RCCGGY 1 cut(s) 188
BsrGI TGTACA 1 cut(s) 462
BsrI ACTGG 1 cut(s) 67
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 2 cut(s) 41, 226
BssMI GATC 3 cut(s) 328, 585, 696
BssNI GRCGYC 1 cut(s) 223
Bst2UI CCWGG 3 cut(s) 42, 98, 713
Bst4CI ACNGT 4 cut(s) 20, 34, 536, 777
Bst6I CTCTTC 1 cut(s) 610
BstACI GRCGYC 1 cut(s) 223
BstAUI TGTACA 1 cut(s) 462
BstC8I GCNNGC 4 cut(s) 88, 190, 525, 709
BstDEI CTNAG 1 cut(s) 264
BstDSI CCRYGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 721
BstFNI CGCG 4 cut(s) 228, 251, 608, 645
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 2 cut(s) 188, 645
BstKTI GATC 3 cut(s) 331, 588, 699
BstMBI GATC 3 cut(s) 328, 585, 696
BstMWI GCNNNNNNNGC 6 cut(s) 248, 254, 269, 281, 490, 815
BstNI CCWGG 3 cut(s) 42, 98, 713
BstSCI CCNGG 3 cut(s) 40, 96, 711
BstSFI CTRYAG 2 cut(s) 30, 451
BstUI CGCG 4 cut(s) 228, 251, 608, 645
BstV1I GCAGC 5 cut(s) 111, 259, 437, 539, 859
BsuRI GGCC 2 cut(s) 101, 711
BtgI CCRYGG 1 cut(s) 226
BtsCI GGATG 1 cut(s) 721
BtsI GCAGTG 1 cut(s) 460
BtsIMutI CAGTG 2 cut(s) 460, 541
Cac8I GCNNGC 4 cut(s) 88, 190, 525, 709
CciI TCATGA 1 cut(s) 306
CfoI GCGC 2 cut(s) 188, 645
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 1 cut(s) 100
Cfr42I CCGCGG 1 cut(s) 229
CseI GACGC 2 cut(s) 231, 278
Csp6I GTAC 2 cut(s) 463, 773
CviAII CATG 4 cut(s) 280, 307, 583, 803
CviQI GTAC 2 cut(s) 463, 773
DdeI CTNAG 1 cut(s) 264
DpnI GATC 3 cut(s) 330, 587, 698
DpnII GATC 3 cut(s) 328, 585, 696
Eam1104I CTCTTC 1 cut(s) 610
EarI CTCTTC 1 cut(s) 610
Ecl136II GAGCTC 1 cut(s) 751
Eco24I GRGCYC 2 cut(s) 351, 753
Eco53kI GAGCTC 1 cut(s) 751
EcoICRI GAGCTC 1 cut(s) 751
EcoO109I RGGNCCY 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 40, 96, 711
EcoT22I ATGCAT 1 cut(s) 760
EcoT38I GRGCYC 2 cut(s) 351, 753
FaeI CATG 4 cut(s) 283, 310, 586, 806
FaiI YATR 8 cut(s) 173, 281, 308, 336, 496, 584, 756, 804
FaqI GGGAC 2 cut(s) 58, 356
FatI CATG 4 cut(s) 279, 306, 582, 802
FauI CCCGC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 585
FblI GTMKAC 1 cut(s) 220
Fnu4HI GCNGC 8 cut(s) 125, 226, 229, 249, 273, 451, 528, 873
FokI GGATG 1 cut(s) 728
FriOI GRGCYC 2 cut(s) 351, 753
Fsp4HI GCNGC 8 cut(s) 125, 226, 229, 249, 273, 451, 528, 873
GlaI GCGC 2 cut(s) 187, 644
GluI GCNGC 8 cut(s) 125, 226, 229, 249, 273, 451, 528, 873
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 2 cut(s) 101, 711
HapII CCGG 2 cut(s) 189, 882
HgaI GACGC 2 cut(s) 231, 278
HhaI GCGC 2 cut(s) 188, 645
Hin1I GRCGYC 1 cut(s) 223
Hin1II CATG 4 cut(s) 283, 310, 586, 806
Hin6I GCGC 2 cut(s) 186, 643
HinP1I GCGC 2 cut(s) 186, 643
HincII GTYRAC 2 cut(s) 205, 221
HindII GTYRAC 2 cut(s) 205, 221
HinfI GANTC 2 cut(s) 46, 206
HpaII CCGG 2 cut(s) 189, 882
HphI GGTGA 3 cut(s) 124, 771, 836
Hpy166II GTNNAC 2 cut(s) 205, 221
Hpy188I TCNGA 2 cut(s) 328, 701
Hpy188III TCNNGA 3 cut(s) 62, 73, 307
Hpy8I GTNNAC 2 cut(s) 205, 221
Hpy99I CGWCG 2 cut(s) 119, 225
HpyAV CCTTC 2 cut(s) 168, 379
HpyCH4III ACNGT 4 cut(s) 20, 34, 536, 777
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 5 cut(s) 428, 453, 527, 560, 758
HpyF10VI GCNNNNNNNGC 6 cut(s) 248, 254, 269, 281, 490, 815
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 1 cut(s) 201
Hsp92I GRCGYC 1 cut(s) 223
Hsp92II CATG 4 cut(s) 283, 310, 586, 806
HspAI GCGC 2 cut(s) 186, 643
KroI GCCGGC 1 cut(s) 188
KroNI GCCGGC 1 cut(s) 190
Ksp22I TGATCA 1 cut(s) 585
KspI CCGCGG 1 cut(s) 229
Kzo9I GATC 3 cut(s) 328, 585, 696
LmnI GCTCC 4 cut(s) 95, 159, 474, 647
Lsp1109I GCAGC 5 cut(s) 111, 259, 437, 539, 859
LweI GCATC 2 cut(s) 529, 793
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 777, 832
MalI GATC 3 cut(s) 330, 587, 698
MbiI CCGCTC 1 cut(s) 90
MboI GATC 3 cut(s) 328, 585, 696
MboII GAAGA 2 cut(s) 627, 848
MhlI GDGCHC 2 cut(s) 351, 753
MluCI AATT 4 cut(s) 167, 466, 500, 842
MlyI GAGTC 2 cut(s) 40, 200
MmeI TCCRAC 1 cut(s) 379
Mph1103I ATGCAT 1 cut(s) 760
MroNI GCCGGC 1 cut(s) 188
MseI TTAA 2 cut(s) 633, 892
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 2 cut(s) 228, 872
MspI CCGG 2 cut(s) 189, 882
MspR9I CCNGG 3 cut(s) 42, 98, 713
Mva1269I GAATGC 1 cut(s) 852
MvaI CCWGG 3 cut(s) 42, 98, 713
MvnI CGCG 4 cut(s) 228, 251, 608, 645
MwoI GCNNNNNNNGC 6 cut(s) 248, 254, 269, 281, 490, 815
NaeI GCCGGC 1 cut(s) 190
NdeII GATC 3 cut(s) 328, 585, 696
NgoMIV GCCGGC 1 cut(s) 188
NlaIII CATG 4 cut(s) 283, 310, 586, 806
NmeAIII GCCGAG 1 cut(s) 238
NmuCI GTSAC 1 cut(s) 777
NsiI ATGCAT 1 cut(s) 760
PagI TCATGA 1 cut(s) 306
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 1 cut(s) 852
PdiI GCCGGC 1 cut(s) 190
PkrI GCNGC 8 cut(s) 126, 227, 230, 250, 274, 452, 529, 874
PleI GAGTC 2 cut(s) 40, 200
PpsI GAGTC 2 cut(s) 40, 200
Psp124BI GAGCTC 1 cut(s) 753
Psp6I CCWGG 3 cut(s) 40, 96, 711
PspGI CCWGG 3 cut(s) 40, 96, 711
PspPI GGNCC 1 cut(s) 100
PstI CTGCAG 1 cut(s) 455
RsaI GTAC 2 cut(s) 464, 774
RsaNI GTAC 2 cut(s) 463, 773
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 753
SacII CCGCGG 1 cut(s) 229
SalI GTCGAC 1 cut(s) 219
SaqAI TTAA 2 cut(s) 633, 892
SatI GCNGC 8 cut(s) 125, 226, 229, 249, 273, 451, 528, 873
Sau3AI GATC 3 cut(s) 328, 585, 696
Sau96I GGNCC 1 cut(s) 100
SchI GAGTC 2 cut(s) 40, 200
ScrFI CCNGG 3 cut(s) 42, 98, 713
SduI GDGCHC 2 cut(s) 351, 753
SetI ASST 9 cut(s) 204, 318, 390, 525, 652, 706, 753, 793, 811
SfaNI GCATC 2 cut(s) 529, 793
SfcI CTRYAG 2 cut(s) 30, 451
Sfr303I CCGCGG 1 cut(s) 229
SgrBI CCGCGG 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 1 cut(s) 60
SmoI CTYRAG 1 cut(s) 60
Sse9I AATT 4 cut(s) 167, 466, 500, 842
SsiI CCGC 9 cut(s) 88, 135, 226, 228, 249, 606, 645, 782, 870
SstI GAGCTC 1 cut(s) 753
StyD4I CCNGG 3 cut(s) 40, 96, 711
TaaI ACNGT 4 cut(s) 20, 34, 536, 777
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 220
TasI AATT 4 cut(s) 167, 466, 500, 842
TatI WGTACW 2 cut(s) 462, 772
TauI GCSGC 3 cut(s) 228, 231, 251
Tru1I TTAA 2 cut(s) 633, 892
Tru9I TTAA 2 cut(s) 633, 892
TscAI CASTG 2 cut(s) 460, 541
TseFI GTSAC 1 cut(s) 777
TseI GCWGC 5 cut(s) 124, 272, 450, 527, 872
Tsp45I GTSAC 1 cut(s) 777
TspDTI ATGAA 3 cut(s) 160, 286, 819
TspGWI ACGGA 2 cut(s) 82, 103
TspRI CASTG 2 cut(s) 460, 541
XapI RAATTY 2 cut(s) 167, 842
XmiI GTMKAC 1 cut(s) 220
Zsp2I ATGCAT 1 cut(s) 760
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.