Rmu_sc0001144.1_g000038

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001144.1
OG0000446
Physical Location & Seq
Reverse (-)
125817 .. 128559
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001144.1_g000038.1.cds

Sequence Viewer

Length: 1341 bp
atggccctattgccaaacctcccagatgtcctgatcatccaaccaccaccagccttcatatctttcgaatccgacttcacccagaagttcaacatcctccgcgcatgggagtcgccactccctctggaccagttcctcaccacccacgcccaatcagtccatgccctcctctgccgcgccgagactccgatcaacgccgacgtcctccggctgctaccggccctgaagatcgtggtgtccgccagcaccggcgccgaccaagtggacaaagccgagtgccggcggcgtgggattgctgtgaccaacgcaggaaatgcgtacgcggaagatgttgctgatatcgctgttgggttgttcatcgatgtgtttcggaagatctcagcagctgattggtatgtgagaaaagggctttggagtagcagtggagattatcctcttggttccaagataggtggaaagcgagttggtatcgttggattgggaagcattggctatgaagttgccaaaagactcgaggcctttggctgcaagatctcatacaactcacgaagcgaaaggccatctttgtcatacaccttctattccaatgtcagtgaacttgcagcagacagtgacgctctcgttatctgttgtgggttaacagaccagacccgccacatgatcaacaaggaagtcttatctgcattgggaaaagatggagtggtagttaatataggccgtggggcaattattgatgagaaggaattggtcaagtgtttggtgaatggagagattagaggtgctggtttggatgtgtatgagaatgagcctcatataccgaaagagctcgtggcaatggacagcgttgttctgtctccacataaggctgtttatacagtagagtctgtcaagaaaggagggtgtcaaaatttcacttcgtcagatctcaggtgtgcagcagtagggtcgtcgtgctacccaggtcgctcagaatatggtcagattgaagaccaaggttggatggtgacaaaggctactggaagagatcgtcgcggctgctgtggagagacaacgtggtcgacggagcaagctttttggtgggatgttgtgctgttcaagcttgggccatttgaaaatgatatgaatgccagtcggttgcaatgggcatcctcgcctgccctgagcactgggctcgaaacgtcgtcgtcttgttcgaagctgggttttgtgaactttcgcgaaggcggttgtggagatagcttgaagacgaataagggtttgtttgtgataaaggcgatggatgtggagggtgcgagaaccgtgaatttgaatgggcatgctggggattcaggtagtttttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

446

Amino Acids

48.19

Weight (kDa)

5.81

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1054
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 1058
AccII CGCG 5 cut(s) 102, 177, 323, 1032, 1218
AciI CCGC 8 cut(s) 100, 175, 240, 283, 323, 652, 1032, 1224
AcsI RAATTY 2 cut(s) 907, 1303
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 106, 279
AgsI TTSAA 6 cut(s) 91, 986, 1096, 1112, 1243, 1309
AjnI CCWGG 1 cut(s) 958
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 6 cut(s) 386, 826, 1070, 1099, 1198, 1239
AluI AGCT 6 cut(s) 386, 826, 1070, 1099, 1198, 1239
Alw21I GWGCWC 2 cut(s) 828, 1166
Alw26I GTCTC 3 cut(s) 176, 858, 1040
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 6 cut(s) 3, 219, 516, 557, 715, 1103
ApeKI GCWGC 6 cut(s) 211, 383, 525, 602, 935, 1035
ApoI RAATTY 2 cut(s) 907, 1303
AspLEI GCGC 3 cut(s) 104, 179, 254
AspS9I GGNCC 4 cut(s) 4, 127, 220, 1103
AsuHPI GGTGA 4 cut(s) 70, 130, 772, 1015
AsuII TTCGAA 2 cut(s) 66, 1193
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 2 cut(s) 828, 1173
BauI CACGAG 1 cut(s) 827
BbsI GAAGAC 2 cut(s) 993, 1250
Bbv12I GWGCWC 2 cut(s) 828, 1166
BbvI GCAGC 6 cut(s) 198, 395, 512, 614, 947, 1022
BccI CCATC 4 cut(s) 568, 689, 994, 1270
BceAI ACGGC 1 cut(s) 702
BcgI CGANNNNNNTGC 6 cut(s) 93, 127, 927, 961, 1153, 1187
BciT130I CCWGG 1 cut(s) 960
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 3 cut(s) 176, 858, 1040
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 3 cut(s) 375, 531, 922
BisI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 936, 1033, 1036
BlsI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 937, 1034, 1037
Bme1390I CCNGG 1 cut(s) 960
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 4 cut(s) 4, 127, 220, 1103
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 1 cut(s) 960
BmrI ACTGGG 1 cut(s) 1176
BmsI GCATC 1 cut(s) 1154
BmuI ACTGGG 1 cut(s) 1176
BpiI GAAGAC 2 cut(s) 993, 1250
Bpu10I CCTNAGC 1 cut(s) 1160
Bpu14I TTCGAA 2 cut(s) 66, 1193
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaJI CCNNGG 3 cut(s) 718, 958, 991
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 279
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 4 cut(s) 130, 1021, 1128, 1171
Bse3DI GCAATG 2 cut(s) 840, 1145
BseBI CCWGG 1 cut(s) 960
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 718, 958, 991
BseGI GGATG 7 cut(s) 36, 93, 796, 1005, 1087, 1145, 1285
BseLI CCNNNNNNNGG 2 cut(s) 106, 279
BseMI GCAATG 2 cut(s) 840, 1145
BseMII CTCAG 4 cut(s) 393, 940, 981, 1151
BseNI ACTGG 4 cut(s) 130, 1021, 1128, 1171
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 198, 395, 512, 614, 947, 1022
BseYI CCCAGC 2 cut(s) 1198, 1319
BsgI GTGCAG 1 cut(s) 954
Bsh1236I CGCG 5 cut(s) 102, 177, 323, 1032, 1218
BshFI GGCC 6 cut(s) 5, 221, 518, 559, 717, 1105
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 828, 1166
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 208, 218, 249, 280
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 106, 279
BsmAI GTCTC 3 cut(s) 176, 858, 1040
BsmI GAATGC 1 cut(s) 1129
BsnI GGCC 6 cut(s) 5, 221, 518, 559, 717, 1105
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 2 cut(s) 66, 1193
Bsp1286I GDGCHC 3 cut(s) 828, 1166, 1173
Bsp143I GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
Bsp68I TCGCGA 1 cut(s) 1218
BspACI CCGC 8 cut(s) 100, 175, 240, 283, 323, 652, 1032, 1224
BspANI GGCC 6 cut(s) 5, 221, 518, 559, 717, 1105
BspCNI CTCAG 4 cut(s) 392, 939, 980, 1152
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 5 cut(s) 102, 177, 323, 1032, 1218
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 2 cut(s) 66, 1193
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 2 cut(s) 840, 1145
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 4 cut(s) 130, 1021, 1128, 1171
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssECI CCNNGG 3 cut(s) 718, 958, 991
BssMI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
BssNI GRCGYC 2 cut(s) 201, 252
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 1 cut(s) 991
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 1 cut(s) 960
Bst4CI ACNGT 3 cut(s) 611, 877, 1300
Bst6I CTCTTC 1 cut(s) 1015
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 2 cut(s) 66, 1193
BstC8I GCNNGC 5 cut(s) 244, 281, 1068, 1155, 1317
BstDEI CTNAG 4 cut(s) 379, 926, 967, 1160
BstDSI CCRYGG 1 cut(s) 718
BstF5I GGATG 7 cut(s) 36, 93, 796, 1005, 1087, 1145, 1285
BstFNI CGCG 5 cut(s) 102, 177, 323, 1032, 1218
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 104, 179, 254
BstKTI GATC 8 cut(s) 36, 192, 231, 378, 534, 663, 925, 1027
BstMAI GTCTC 3 cut(s) 176, 858, 1040
BstMBI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 314, 341, 1096
BstNI CCWGG 1 cut(s) 960
BstNSI RCATGY 1 cut(s) 1319
BstSCI CCNGG 1 cut(s) 958
BstUI CGCG 5 cut(s) 102, 177, 323, 1032, 1218
BstV1I GCAGC 6 cut(s) 198, 395, 512, 614, 947, 1022
BstV2I GAAGAC 2 cut(s) 993, 1250
BstX2I RGATCY 3 cut(s) 375, 531, 922
BstYI RGATCY 3 cut(s) 375, 531, 922
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 6 cut(s) 5, 221, 518, 559, 717, 1105
BsuTUI ATCGAT 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 718
BtgZI GCGATG 1 cut(s) 1289
BtsCI GGATG 7 cut(s) 36, 93, 796, 1005, 1087, 1145, 1285
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 4 cut(s) 427, 598, 616, 1164
BtuMI TCGCGA 1 cut(s) 1218
Cac8I GCNNGC 5 cut(s) 244, 281, 1068, 1155, 1317
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 3 cut(s) 104, 179, 254
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 4 cut(s) 4, 127, 220, 1103
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 4 cut(s) 105, 161, 658, 1316
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 4 cut(s) 379, 926, 967, 1160
DinI GGCGCC 1 cut(s) 253
DpnI GATC 8 cut(s) 35, 191, 230, 377, 533, 662, 924, 1026
DpnII GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
DrdI GACNNNNNNGTC 1 cut(s) 1054
DseDI GACNNNNNNGTC 1 cut(s) 1054
Eam1104I CTCTTC 1 cut(s) 1015
EarI CTCTTC 1 cut(s) 1015
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 991
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 2 cut(s) 828, 1173
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
EcoICRI GAGCTC 1 cut(s) 826
EcoRII CCWGG 1 cut(s) 958
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 991
EcoT38I GRGCYC 2 cut(s) 828, 1173
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 991
FaeI CATG 4 cut(s) 108, 164, 661, 1319
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 4 cut(s) 104, 160, 657, 1315
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 33, 660
FblI GTMKAC 1 cut(s) 1058
Fnu4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 936, 1033, 1036
FokI GGATG 7 cut(s) 23, 80, 803, 1012, 1094, 1132, 1292
FriOI GRGCYC 2 cut(s) 828, 1173
Fsp4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 936, 1033, 1036
GlaI GCGC 3 cut(s) 103, 178, 253
GluI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 936, 1033, 1036
GsaI CCCAGC 2 cut(s) 1202, 1323
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 6 cut(s) 5, 221, 518, 559, 717, 1105
HapII CCGG 4 cut(s) 208, 218, 249, 280
HgaI GACGC 1 cut(s) 623
HhaI GCGC 3 cut(s) 104, 179, 254
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 4 cut(s) 108, 164, 661, 1319
Hin6I GCGC 3 cut(s) 102, 177, 252
HinP1I GCGC 3 cut(s) 102, 177, 252
HincII GTYRAC 2 cut(s) 639, 1059
HindII GTYRAC 2 cut(s) 639, 1059
HindIII AAGCTT 2 cut(s) 1068, 1097
HinfI GANTC 6 cut(s) 68, 110, 184, 510, 881, 1325
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 4 cut(s) 208, 218, 249, 280
HphI GGTGA 4 cut(s) 70, 130, 772, 1015
Hpy166II GTNNAC 5 cut(s) 265, 596, 639, 1059, 1210
Hpy188I TCNGA 6 cut(s) 73, 189, 372, 922, 970, 981
Hpy188III TCNNGA 5 cut(s) 31, 125, 546, 889, 1217
Hpy8I GTNNAC 5 cut(s) 265, 596, 639, 1059, 1210
Hpy99I CGWCG 6 cut(s) 203, 952, 1032, 1063, 1183, 1186
HpyAV CCTTC 4 cut(s) 64, 586, 733, 1214
HpyCH4III ACNGT 3 cut(s) 611, 877, 1300
HpyCH4IV ACGT 3 cut(s) 201, 1052, 1178
HpyCH4V TGCA 5 cut(s) 528, 602, 683, 935, 1138
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 341, 1096
HpyF3I CTNAG 4 cut(s) 379, 926, 967, 1160
HpySE526I ACGT 3 cut(s) 201, 1052, 1178
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 4 cut(s) 108, 164, 661, 1319
HspAI GCGC 3 cut(s) 102, 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 33, 660
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
LmnI GCTCC 1 cut(s) 1063
Lsp1109I GCAGC 6 cut(s) 198, 395, 512, 614, 947, 1022
LweI GCATC 1 cut(s) 1154
MaeII ACGT 3 cut(s) 201, 1052, 1178
MaeIII GTNAC 3 cut(s) 298, 611, 1003
MalI GATC 8 cut(s) 35, 191, 230, 377, 533, 662, 924, 1026
MboI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
MboII GAAGA 6 cut(s) 238, 338, 385, 998, 1032, 1255
MflI RGATCY 3 cut(s) 375, 531, 922
MhlI GDGCHC 3 cut(s) 828, 1166, 1173
MluCI AATT 4 cut(s) 726, 743, 907, 1303
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 4 cut(s) 119, 178, 504, 890
MmeI TCCRAC 4 cut(s) 64, 96, 454, 977
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 2 cut(s) 638, 708
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 208, 218, 249, 280
MspR9I CCNGG 1 cut(s) 960
Mva1269I GAATGC 1 cut(s) 1129
MvaI CCWGG 1 cut(s) 960
MvnI CGCG 5 cut(s) 102, 177, 323, 1032, 1218
MwoI GCNNNNNNNGC 3 cut(s) 314, 341, 1096
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 4 cut(s) 108, 164, 661, 1319
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 3 cut(s) 298, 611, 1003
NruI TCGCGA 1 cut(s) 1218
NspI RCATGY 1 cut(s) 1319
NspV TTCGAA 2 cut(s) 66, 1193
PaeI GCATGC 1 cut(s) 1319
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 1190
PctI GAATGC 1 cut(s) 1129
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 68, 1325
Pfl23II CGTACG 1 cut(s) 318
PkrI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 937, 1034, 1037
PleI GAGTC 4 cut(s) 118, 178, 504, 889
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 4 cut(s) 118, 178, 504, 889
Psp124BI GAGCTC 1 cut(s) 828
Psp6I CCWGG 1 cut(s) 958
PspFI CCCAGC 2 cut(s) 1198, 1319
PspGI CCWGG 1 cut(s) 958
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 4 cut(s) 4, 127, 220, 1103
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 3 cut(s) 375, 531, 922
PvuII CAGCTG 1 cut(s) 386
RruI TCGCGA 1 cut(s) 1218
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 828
SalI GTCGAC 1 cut(s) 1057
SaqAI TTAA 2 cut(s) 638, 708
SatI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 936, 1033, 1036
Sau3AI GATC 8 cut(s) 33, 189, 228, 375, 531, 660, 922, 1024
Sau96I GGNCC 4 cut(s) 4, 127, 220, 1103
SchI GAGTC 4 cut(s) 119, 178, 504, 890
ScrFI CCNGG 1 cut(s) 960
SduI GDGCHC 3 cut(s) 828, 1166, 1173
SfaNI GCATC 1 cut(s) 1154
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 2 cut(s) 66, 1193
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SphI GCATGC 1 cut(s) 1319
Sse9I AATT 4 cut(s) 726, 743, 907, 1303
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 8 cut(s) 100, 175, 240, 283, 323, 652, 1032, 1224
SspDI GGCGCC 1 cut(s) 251
SstI GAGCTC 1 cut(s) 828
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 958
StyI CCWWGG 1 cut(s) 991
TaaI ACNGT 3 cut(s) 611, 877, 1300
TaiI ACGT 3 cut(s) 204, 1055, 1181
TaqI TCGA 6 cut(s) 66, 360, 513, 1058, 1173, 1193
TasI AATT 4 cut(s) 726, 743, 907, 1303
TauI GCSGC 3 cut(s) 177, 286, 1035
TfiI GAWTC 2 cut(s) 68, 1325
Tru1I TTAA 2 cut(s) 638, 708
Tru9I TTAA 2 cut(s) 638, 708
TscAI CASTG 4 cut(s) 427, 598, 616, 1171
TseFI GTSAC 3 cut(s) 298, 611, 1003
TseI GCWGC 6 cut(s) 211, 383, 525, 602, 935, 1035
Tsp45I GTSAC 3 cut(s) 298, 611, 1003
TspDTI ATGAA 4 cut(s) 46, 346, 510, 1136
TspGWI ACGGA 1 cut(s) 1076
TspRI CASTG 4 cut(s) 427, 598, 616, 1171
VpaK11BI GGWCC 1 cut(s) 127
XapI RAATTY 2 cut(s) 907, 1303
XceI RCATGY 1 cut(s) 1319
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 1058
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.