FvH4_7g12590

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000446
Physical Location & Seq
Forward (+)
11398083 .. 11399854
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12590.t1

Sequence Viewer

Length: 972 bp
ATGGCTCCATTGCCAAACCTTCCAGAAGTCCTAATCATCAACCCACCACCAGCCTTCATCTCTTTCGAATCCGACTTCACCAAAAAGTTCAACATCCTCCGAGCATGGGAATCCCCACTCCCTCTGGACCAGTACCTCACCACCCACGCCCAATCCGCCAAGGCCCTCCTCTGCCGCGCTTTGACTCCGGTCAACGCCGACCTCCTCCGTCAGTTACCGGCCCTGAAGCTCGTGGTGTCCGTCAGCACCGGCGCCAACCACGTGGACAAAGCGGAGTGCCGGCGGCGTGGGATTGCCGTGACCAATGCTGGAAATGCGTACTCGGAAGATGTTGCTGATCTCGCTGTTGGGTTGTTTCTCGATGTGAATAGGAAGATTTCGGCAGCTGATTGGTATGTGAGACGTGGGCTTTGGAGTAGCAAAGGAGACTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCCTTTGGCTGCATCATCTCATACAACTCAAGGAGGGAAAAGCCATCTTTGTCATACCCCTTCTATTCCAATGTCTGTGAACTTGCAGCTAATAATGATGCGCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATTGGCATTGGGAAAATATGGAGTGGTGGTTAACATAGGGCGTGGGGCAATTATTGATGAGAAAGAAATGGTCAAGTGTTTGGTGAACGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGAGCTGGTGGAAATGGACAATGTTGTTCTGTCTCCACATAGGGGTGTTTATACACTAGAATCGTTAAAGAGAGGAAGTGAATTAATGATCGCCAATCTGGCAGCTTTCTTCTCCAATAAACCTCTGGTTACTCCAGCCATGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

324

Amino Acids

35.31

Weight (kDa)

7.57

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 44 - 316 2.6e-22 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 1.7e-47 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 251
AccII CGCG 1 cut(s) 177
AciI CCGC 5 cut(s) 156, 175, 272, 283, 652
AcuI CTGAAG 1 cut(s) 245
AcvI CACGTG 1 cut(s) 262
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 2 cut(s) 134, 320
AfiI CCNNNNNNNGG 2 cut(s) 106, 863
AgsI TTSAA 1 cut(s) 91
AjiI CACGTC 1 cut(s) 404
AluBI AGCT 5 cut(s) 229, 386, 605, 826, 926
AluI AGCT 5 cut(s) 229, 386, 605, 826, 926
Alw26I GTCTC 3 cut(s) 394, 420, 858
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 3 cut(s) 162, 219, 516
ApeKI GCWGC 4 cut(s) 383, 525, 602, 923
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AseI ATTAAT 1 cut(s) 905
AspLEI GCGC 3 cut(s) 179, 254, 619
AspS9I GGNCC 3 cut(s) 127, 163, 220
AsuHPI GGTGA 3 cut(s) 70, 130, 772
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BauI CACGAG 1 cut(s) 230
BbrPI CACGTG 1 cut(s) 262
BbvI GCAGC 4 cut(s) 395, 512, 614, 935
BccI CCATC 1 cut(s) 568
BceAI ACGGC 1 cut(s) 281
BclI TGATCA 1 cut(s) 660
BcoDI GTCTC 3 cut(s) 394, 420, 858
BfaI CTAG 1 cut(s) 878
BfoI RGCGCY 1 cut(s) 255
BglI GCCNNNNNGGC 1 cut(s) 920
BisI GCNGC 6 cut(s) 175, 284, 384, 526, 603, 924
BlsI GCNGC 6 cut(s) 176, 285, 385, 527, 604, 925
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 3 cut(s) 127, 163, 220
BmiI GGNNCC 3 cut(s) 6, 253, 442
BmsI GCATC 2 cut(s) 537, 604
BoxI GACNNNNGTC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
BsaAI YACGTR 1 cut(s) 262
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 2 cut(s) 159, 960
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 863
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 1 cut(s) 130
Bse3DI GCAATG 1 cut(s) 8
BseDI CCNNGG 2 cut(s) 159, 960
BseGI GGATG 3 cut(s) 93, 796, 970
BseLI CCNNNNNNNGG 2 cut(s) 106, 863
BseMI GCAATG 1 cut(s) 8
BseNI ACTGG 1 cut(s) 130
BseRI GAGGAG 2 cut(s) 158, 194
BseXI GCAGC 4 cut(s) 395, 512, 614, 935
Bsh1236I CGCG 1 cut(s) 177
BshFI GGCC 3 cut(s) 164, 221, 518
BshNI GGYRCC 1 cut(s) 251
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 188, 218, 249, 280
BslI CCNNNNNNNGG 2 cut(s) 106, 863
BsmAI GTCTC 3 cut(s) 394, 420, 858
BsmBI CGTCTC 1 cut(s) 394
BsnI GGCC 3 cut(s) 164, 221, 518
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 4 cut(s) 337, 660, 771, 909
Bsp19I CCATGG 1 cut(s) 960
BspACI CCGC 5 cut(s) 156, 175, 272, 283, 652
BspANI GGCC 3 cut(s) 164, 221, 518
BspFNI CGCG 1 cut(s) 177
BspLI GGNNCC 3 cut(s) 6, 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 8
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 1 cut(s) 130
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssECI CCNNGG 2 cut(s) 159, 960
BssMI GATC 4 cut(s) 337, 660, 771, 909
BssNI GRCGYC 1 cut(s) 252
BssSI CACGAG 1 cut(s) 230
BssT1I CCWWGG 2 cut(s) 159, 960
Bst2BI CACGAG 1 cut(s) 230
BstACI GRCGYC 1 cut(s) 252
BstBAI YACGTR 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 1 cut(s) 281
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 3 cut(s) 93, 796, 970
BstFNI CGCG 1 cut(s) 177
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 179, 254, 619
BstKTI GATC 4 cut(s) 340, 663, 774, 912
BstMAI GTCTC 3 cut(s) 394, 420, 858
BstMBI GATC 4 cut(s) 337, 660, 771, 909
BstMWI GCNNNNNNNGC 4 cut(s) 155, 314, 341, 920
BstPAI GACNNNNGTC 1 cut(s) 188
BstUI CGCG 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 395, 512, 614, 935
BstXI CCANNNNNNTGG 1 cut(s) 262
BsuRI GGCC 3 cut(s) 164, 221, 518
BtgI CCRYGG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 3 cut(s) 93, 796, 970
Cac8I GCNNGC 1 cut(s) 281
CfoI GCGC 3 cut(s) 179, 254, 619
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 3 cut(s) 127, 163, 220
Csp6I GTAC 2 cut(s) 133, 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 658, 961
CviQI GTAC 2 cut(s) 133, 319
DinI GGCGCC 1 cut(s) 253
DpnI GATC 4 cut(s) 339, 662, 773, 911
DpnII GATC 4 cut(s) 337, 660, 771, 909
EciI GGCGGA 1 cut(s) 145
Eco130I CCWWGG 2 cut(s) 159, 960
Eco147I AGGCCT 1 cut(s) 518
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco72I CACGTG 1 cut(s) 262
Eco88I CYCGRG 1 cut(s) 512
EcoO109I RGGNCCY 1 cut(s) 163
EcoT14I CCWWGG 2 cut(s) 159, 960
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 2 cut(s) 159, 960
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 3 cut(s) 108, 661, 964
FalI AAGNNNNNCTT 2 cut(s) 659, 691
FatI CATG 3 cut(s) 104, 657, 960
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 1 cut(s) 660
Fnu4HI GCNGC 6 cut(s) 175, 284, 384, 526, 603, 924
FokI GGATG 2 cut(s) 80, 803
Fsp4HI GCNGC 6 cut(s) 175, 284, 384, 526, 603, 924
FspBI CTAG 1 cut(s) 878
GlaI GCGC 3 cut(s) 178, 253, 618
GluI GCNGC 6 cut(s) 175, 284, 384, 526, 603, 924
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 3 cut(s) 164, 221, 518
HapII CCGG 4 cut(s) 188, 218, 249, 280
HhaI GCGC 3 cut(s) 179, 254, 619
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 3 cut(s) 108, 661, 964
Hin6I GCGC 3 cut(s) 177, 252, 617
HinP1I GCGC 3 cut(s) 177, 252, 617
HincII GTYRAC 2 cut(s) 193, 709
HindII GTYRAC 2 cut(s) 193, 709
HinfI GANTC 5 cut(s) 68, 110, 184, 510, 881
HpaI GTTAAC 1 cut(s) 709
HpaII CCGG 4 cut(s) 188, 218, 249, 280
HphI GGTGA 3 cut(s) 70, 130, 772
Hpy166II GTNNAC 5 cut(s) 193, 265, 596, 709, 763
Hpy188I TCNGA 4 cut(s) 73, 101, 325, 776
Hpy188III TCNNGA 3 cut(s) 23, 125, 359
Hpy8I GTNNAC 5 cut(s) 193, 265, 596, 709, 763
HpyAV CCTTC 3 cut(s) 29, 64, 586
HpyCH4IV ACGT 2 cut(s) 261, 403
HpyCH4V TGCA 3 cut(s) 528, 602, 635
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 314, 341, 920
HpySE526I ACGT 2 cut(s) 261, 403
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 3 cut(s) 108, 661, 964
HspAI GCGC 3 cut(s) 177, 252, 617
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 1 cut(s) 660
KspAI GTTAAC 1 cut(s) 709
Kzo9I GATC 4 cut(s) 337, 660, 771, 909
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 395, 512, 614, 935
LweI GCATC 2 cut(s) 537, 604
MaeI CTAG 1 cut(s) 878
MaeII ACGT 2 cut(s) 261, 403
MaeIII GTNAC 3 cut(s) 213, 298, 949
MalI GATC 4 cut(s) 339, 662, 773, 911
MboI GATC 4 cut(s) 337, 660, 771, 909
MboII GAAGA 3 cut(s) 338, 385, 922
MluCI AATT 2 cut(s) 726, 902
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 178, 504
MmeI TCCRAC 2 cut(s) 96, 454
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 3 cut(s) 708, 887, 905
MslI CAYNNNNRTG 1 cut(s) 864
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 4 cut(s) 188, 218, 249, 280
MvnI CGCG 1 cut(s) 177
MwoI GCNNNNNNNGC 4 cut(s) 155, 314, 341, 920
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NcoI CCATGG 1 cut(s) 960
NdeII GATC 4 cut(s) 337, 660, 771, 909
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 3 cut(s) 108, 661, 964
NlaIV GGNNCC 3 cut(s) 6, 253, 442
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 3 cut(s) 68, 110, 881
PkrI GCNGC 6 cut(s) 176, 285, 385, 527, 604, 925
PleI GAGTC 2 cut(s) 178, 504
PluTI GGCGCC 1 cut(s) 255
PmaCI CACGTG 1 cut(s) 262
PmlI CACGTG 1 cut(s) 262
PpsI GAGTC 2 cut(s) 178, 504
Ppu21I YACGTR 1 cut(s) 262
PshAI GACNNNNGTC 1 cut(s) 188
PshBI ATTAAT 1 cut(s) 905
PspCI CACGTG 1 cut(s) 262
PspN4I GGNNCC 3 cut(s) 6, 253, 442
PspPI GGNCC 3 cut(s) 127, 163, 220
PspXI VCTCGAGB 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 134, 320
RsaNI GTAC 2 cut(s) 133, 319
RseI CAYNNNNRTG 1 cut(s) 864
SaqAI TTAA 3 cut(s) 708, 887, 905
SatI GCNGC 6 cut(s) 175, 284, 384, 526, 603, 924
Sau3AI GATC 4 cut(s) 337, 660, 771, 909
Sau96I GGNCC 3 cut(s) 127, 163, 220
SchI GAGTC 2 cut(s) 178, 504
SfaNI GCATC 2 cut(s) 537, 604
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 864
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 2 cut(s) 726, 902
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 5 cut(s) 156, 175, 272, 283, 652
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 1 cut(s) 878
StuI AGGCCT 1 cut(s) 518
StyI CCWWGG 2 cut(s) 159, 960
TaiI ACGT 2 cut(s) 264, 406
TaqI TCGA 3 cut(s) 66, 360, 513
TasI AATT 2 cut(s) 726, 902
TauI GCSGC 2 cut(s) 177, 286
TfiI GAWTC 3 cut(s) 68, 110, 881
Tru1I TTAA 3 cut(s) 708, 887, 905
Tru9I TTAA 3 cut(s) 708, 887, 905
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 4 cut(s) 383, 525, 602, 923
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 2 cut(s) 46, 510
TspGWI ACGGA 3 cut(s) 197, 229, 780
VpaK11BI GGWCC 1 cut(s) 127
VspI ATTAAT 1 cut(s) 905
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 512
XspI CTAG 1 cut(s) 878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.