FvH4_7g12580

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000446
Physical Location & Seq
Forward (+)
11391222 .. 11393048
1827 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12580.t1

Sequence Viewer

Length: 1005 bp
ATGACTGGATTGCAATTCTGTGATGCCAAGGATGATGTGCCATCCGAGGAACTCCCACATCTTCTACTCATTCATCCACCTTCATCTTTCACACGTTTCCCACCTGAATTATGTGCAAAGTTCCCTGTCCTGAAAGCTTGGGAGTCGCCACTCCCTCTAGACCAGTTCTTGAGCACCCATGCAGGCTCAATTCAAGCCCTGCTCGTTCGCGGTTATCTTAAGGTTAACGCAGACATCCTTGAGCAGCTACCGGCACTGAAACTCGTCTTGACTATCAGCGTTGGTGTGAACCACATCGACATGGCAGAGTGCCGCCGGCGTGGGATTTCTGTGGCTAATGTGGGAAATGCGTACTCGGAAGATGTTGCTGACATTGCTGTTGGGTTGTATATTGATGTACTTCGAAAACTTTCAGCAGCAGACCGGTATGTAAGACAAGGATTTTGGAGTAGCAAAGGAAATTACCCTCTTGGTTCCAAGTTAGGTGGGAAGCGAGTTGGGATCATTGGACTAGGAAGCATTGGCTCAGAAGTTGCTAAACGACTCGAGGCCTTTGGCTGCATCATCTCATACAACTCAAGGAGGGAAAAGCCATCTTTGTCATACCCCTTCTATCCTAACGTATCTGAACTTGCAGCTAACAGTGATGCCCTCATCATCTGTTGTGCATTAACAGATCAAACCCACCACATGATCAACAAGGAAGTCCTGGCTTTACTTGGAAGAGAGGGTGTGATTGTTAATGTAGGCCGGGGGGCAATAATTGATGAGAAAGAACTGGTGAAGTGTTTGGTGAGTGGGGAAATAAGAGGTGCAGGTTTCGATGTGTTTGAGAATGAGCCTCAAGTTCCTGAAGAGCTCTTTGCATTGGACAATGTTGTTCTTTCCCCACATAAGGCTGTTAGTACTCTGGAATCTTCACAGAGTATGAATGAAATGATGACGGCCAATCTGGAAGCTTTCTTCTCGAATAAACCTCTGGTTTCTCCTGTCATGGAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

335

Amino Acids

36.43

Weight (kDa)

5.39

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 61 - 331 5.6e-23 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 128 - 300 1.1e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 806
AccII CGCG 1 cut(s) 210
AciI CCGC 2 cut(s) 210, 313
AclWI GGATC 1 cut(s) 509
AcoI YGGCCR 1 cut(s) 945
AcuI CTGAAG 1 cut(s) 873
AfaI GTAC 3 cut(s) 353, 399, 907
AfiI CCNNNNNNNGG 1 cut(s) 895
AflII CTTAAG 1 cut(s) 218
AflIII ACRYGT 1 cut(s) 92
AgeI ACCGGT 1 cut(s) 423
AgsI TTSAA 1 cut(s) 194
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 5 cut(s) 137, 247, 638, 859, 959
AluI AGCT 5 cut(s) 137, 247, 638, 859, 959
Alw21I GWGCWC 2 cut(s) 176, 861
AlwI GGATC 1 cut(s) 509
Ama87I CYCGRG 1 cut(s) 545
AoxI GGCC 3 cut(s) 549, 748, 945
ApeKI GCWGC 4 cut(s) 244, 416, 558, 635
ArsI GACNNNNNNTTYG 2 cut(s) 426, 458
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 409
AsuC2I CCSGG 1 cut(s) 752
AsuHPI GGTGA 2 cut(s) 793, 805
AsuII TTCGAA 1 cut(s) 403
AvaI CYCGRG 1 cut(s) 545
BanII GRGCYC 1 cut(s) 861
Bbv12I GWGCWC 2 cut(s) 176, 861
BbvI GCAGC 4 cut(s) 256, 428, 545, 647
BccI CCATC 2 cut(s) 49, 601
BceAI ACGGC 1 cut(s) 960
BciT130I CCWGG 1 cut(s) 710
BclI TGATCA 1 cut(s) 693
BcnI CCSGG 1 cut(s) 752
BfaI CTAG 2 cut(s) 158, 512
BfrI CTTAAG 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 806
BisI GCNGC 5 cut(s) 245, 313, 417, 559, 636
BlsI GCNGC 5 cut(s) 246, 314, 418, 560, 637
BmcAI AGTACT 1 cut(s) 907
Bme1390I CCNGG 2 cut(s) 710, 752
BmeT110I CYCGRG 1 cut(s) 545
BmiI GGNNCC 1 cut(s) 475
BmrFI CCNGG 2 cut(s) 710, 752
BmsI GCATC 3 cut(s) 13, 570, 637
Bpu14I TTCGAA 1 cut(s) 403
BpuEI CTTGAG 4 cut(s) 190, 260, 562, 828
BpuMI CCSGG 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 27, 45, 751
BsaWI WCCGGW 1 cut(s) 423
Bsc4I CCNNNNNNNGG 1 cut(s) 895
Bse118I RCCGGY 3 cut(s) 250, 315, 423
Bse1I ACTGG 3 cut(s) 10, 163, 783
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 3 cut(s) 27, 45, 751
BseGI GGATG 4 cut(s) 37, 41, 73, 234
BseLI CCNNNNNNNGG 1 cut(s) 895
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 1 cut(s) 540
BseNI ACTGG 3 cut(s) 10, 163, 783
BseXI GCAGC 4 cut(s) 256, 428, 545, 647
BsgI GTGCAG 1 cut(s) 834
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 551, 750, 947
BshTI ACCGGT 1 cut(s) 423
BsiHKAI GWGCWC 2 cut(s) 176, 861
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 4 cut(s) 251, 316, 424, 751
BslI CCNNNNNNNGG 1 cut(s) 895
BsnI GGCC 3 cut(s) 551, 750, 947
BsoBI CYCGRG 1 cut(s) 545
Bsp119I TTCGAA 1 cut(s) 403
Bsp1286I GDGCHC 2 cut(s) 176, 861
Bsp143I GATC 3 cut(s) 501, 676, 693
BspACI CCGC 2 cut(s) 210, 313
BspANI GGCC 3 cut(s) 551, 750, 947
BspCNI CTCAG 1 cut(s) 539
BspFNI CGCG 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 475
BspMI ACCTGC 1 cut(s) 806
BspPI GGATC 1 cut(s) 509
BspQI GCTCTTC 1 cut(s) 849
BspT104I TTCGAA 1 cut(s) 403
BspTI CTTAAG 1 cut(s) 218
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 3 cut(s) 250, 315, 423
BsrI ACTGG 3 cut(s) 10, 163, 783
BssAI RCCGGY 3 cut(s) 250, 315, 423
BssECI CCNNGG 3 cut(s) 27, 45, 751
BssMI GATC 3 cut(s) 501, 676, 693
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 1 cut(s) 644
Bst6I CTCTTC 3 cut(s) 718, 849, 993
BstAFI CTTAAG 1 cut(s) 218
BstBI TTCGAA 1 cut(s) 403
BstC8I GCNNGC 2 cut(s) 184, 317
BstDEI CTNAG 1 cut(s) 526
BstF5I GGATG 4 cut(s) 37, 41, 73, 234
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 3 cut(s) 504, 679, 696
BstMBI GATC 3 cut(s) 501, 676, 693
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 708, 750
BstUI CGCG 1 cut(s) 210
BstV1I GCAGC 4 cut(s) 256, 428, 545, 647
BsuRI GGCC 3 cut(s) 551, 750, 947
BtsCI GGATG 4 cut(s) 37, 41, 73, 234
BtsIMutI CAGTG 2 cut(s) 254, 649
BveI ACCTGC 1 cut(s) 806
Cac8I GCNNGC 2 cut(s) 184, 317
Cfr10I RCCGGY 3 cut(s) 250, 315, 423
Csp6I GTAC 3 cut(s) 352, 398, 906
CspAI ACCGGT 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 466, 501
CviAII CATG 4 cut(s) 179, 301, 691, 994
CviQI GTAC 3 cut(s) 352, 398, 906
DdeI CTNAG 1 cut(s) 526
DpnI GATC 3 cut(s) 503, 678, 695
DpnII GATC 3 cut(s) 501, 676, 693
EaeI YGGCCR 1 cut(s) 945
Eam1104I CTCTTC 3 cut(s) 718, 849, 993
EarI CTCTTC 3 cut(s) 718, 849, 993
Ecl136II GAGCTC 1 cut(s) 859
Eco130I CCWWGG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 551
Eco24I GRGCYC 1 cut(s) 861
Eco53kI GAGCTC 1 cut(s) 859
Eco57I CTGAAG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 859
EcoRII CCWGG 1 cut(s) 708
EcoT14I CCWWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 861
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 4 cut(s) 182, 304, 694, 997
FatI CATG 4 cut(s) 178, 300, 690, 993
FbaI TGATCA 1 cut(s) 693
Fnu4HI GCNGC 5 cut(s) 245, 313, 417, 559, 636
FokI GGATG 4 cut(s) 28, 44, 60, 221
FriOI GRGCYC 1 cut(s) 861
Fsp4HI GCNGC 5 cut(s) 245, 313, 417, 559, 636
FspBI CTAG 2 cut(s) 158, 512
GluI GCNGC 5 cut(s) 245, 313, 417, 559, 636
HaeIII GGCC 3 cut(s) 551, 750, 947
HapII CCGG 4 cut(s) 251, 316, 424, 751
Hin1II CATG 4 cut(s) 182, 304, 694, 997
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HindIII AAGCTT 2 cut(s) 135, 957
HinfI GANTC 3 cut(s) 143, 543, 914
HpaI GTTAAC 1 cut(s) 226
HpaII CCGG 4 cut(s) 251, 316, 424, 751
HphI GGTGA 2 cut(s) 793, 805
Hpy166II GTNNAC 2 cut(s) 226, 289
Hpy188I TCNGA 4 cut(s) 46, 358, 529, 628
Hpy188III TCNNGA 8 cut(s) 130, 158, 169, 268, 851, 911, 953, 967
Hpy8I GTNNAC 2 cut(s) 226, 289
HpyAV CCTTC 2 cut(s) 90, 619
HpyCH4III ACNGT 1 cut(s) 644
HpyCH4IV ACGT 2 cut(s) 94, 621
HpyCH4V TGCA 8 cut(s) 13, 116, 182, 561, 635, 668, 815, 866
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 1 cut(s) 526
HpySE526I ACGT 2 cut(s) 94, 621
Hsp92II CATG 4 cut(s) 182, 304, 694, 997
KroI GCCGGC 1 cut(s) 315
KroNI GCCGGC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 693
KspAI GTTAAC 1 cut(s) 226
Kzo9I GATC 3 cut(s) 501, 676, 693
LguI GCTCTTC 1 cut(s) 849
Lsp1109I GCAGC 4 cut(s) 256, 428, 545, 647
LweI GCATC 3 cut(s) 13, 570, 637
MaeI CTAG 2 cut(s) 158, 512
MaeII ACGT 2 cut(s) 94, 621
MalI GATC 3 cut(s) 503, 678, 695
MboI GATC 3 cut(s) 501, 676, 693
MboII GAAGA 6 cut(s) 53, 371, 735, 866, 909, 955
MhlI GDGCHC 2 cut(s) 176, 861
MluCI AATT 5 cut(s) 14, 107, 189, 460, 762
MlyI GAGTC 2 cut(s) 152, 537
MreI CGCCGGCG 1 cut(s) 315
MroNI GCCGGC 1 cut(s) 315
MroXI GAANNNNTTC 1 cut(s) 409
MseI TTAA 4 cut(s) 219, 225, 671, 741
MslI CAYNNNNRTG 1 cut(s) 299
MspCI CTTAAG 1 cut(s) 218
MspI CCGG 4 cut(s) 251, 316, 424, 751
MspR9I CCNGG 2 cut(s) 710, 752
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 1 cut(s) 210
MwoI GCNNNNNNNGC 1 cut(s) 374
NaeI GCCGGC 1 cut(s) 317
NciI CCSGG 1 cut(s) 752
NdeII GATC 3 cut(s) 501, 676, 693
NgoMIV GCCGGC 1 cut(s) 315
NlaIII CATG 4 cut(s) 182, 304, 694, 997
NlaIV GGNNCC 1 cut(s) 475
NspV TTCGAA 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 545
PceI AGGCCT 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 849
PdiI GCCGGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 409
PfeI GAWTC 1 cut(s) 914
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 5 cut(s) 246, 314, 418, 560, 637
PleI GAGTC 2 cut(s) 151, 537
PpsI GAGTC 2 cut(s) 151, 537
Psp124BI GAGCTC 1 cut(s) 861
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 1 cut(s) 475
PspXI VCTCGAGB 1 cut(s) 545
RsaI GTAC 3 cut(s) 353, 399, 907
RsaNI GTAC 3 cut(s) 352, 398, 906
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 861
SapI GCTCTTC 1 cut(s) 849
SaqAI TTAA 4 cut(s) 219, 225, 671, 741
SatI GCNGC 5 cut(s) 245, 313, 417, 559, 636
Sau3AI GATC 3 cut(s) 501, 676, 693
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 2 cut(s) 152, 537
ScrFI CCNGG 2 cut(s) 710, 752
SduI GDGCHC 2 cut(s) 176, 861
SfaNI GCATC 3 cut(s) 13, 570, 637
Sfr274I CTCGAG 1 cut(s) 545
SfuI TTCGAA 1 cut(s) 403
SgrAI CRCCGGYG 1 cut(s) 315
SlaI CTCGAG 1 cut(s) 545
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 6 cut(s) 169, 218, 239, 545, 577, 843
SmoI CTYRAG 6 cut(s) 169, 218, 239, 545, 577, 843
Sse9I AATT 5 cut(s) 14, 107, 189, 460, 762
SseBI AGGCCT 1 cut(s) 551
SsiI CCGC 2 cut(s) 210, 313
SspMI CTAG 2 cut(s) 158, 512
SstI GAGCTC 1 cut(s) 861
StuI AGGCCT 1 cut(s) 551
StyD4I CCNGG 2 cut(s) 708, 750
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 644
TaiI ACGT 2 cut(s) 97, 624
TaqI TCGA 5 cut(s) 297, 403, 546, 822, 968
TasI AATT 5 cut(s) 14, 107, 189, 460, 762
TatI WGTACW 2 cut(s) 397, 905
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 1 cut(s) 914
Tru1I TTAA 4 cut(s) 219, 225, 671, 741
Tru9I TTAA 4 cut(s) 219, 225, 671, 741
TscAI CASTG 2 cut(s) 261, 649
TseI GCWGC 4 cut(s) 244, 416, 558, 635
TspDTI ATGAA 4 cut(s) 62, 72, 944, 948
TspRI CASTG 2 cut(s) 261, 649
Vha464I CTTAAG 1 cut(s) 218
XbaI TCTAGA 1 cut(s) 157
XhoI CTCGAG 1 cut(s) 545
XmnI GAANNNNTTC 1 cut(s) 409
XspI CTAG 2 cut(s) 158, 512
ZrmI AGTACT 1 cut(s) 907
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.