AT2G45630

Glyoxylate hydroxypyruvate reductase HPR3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0000446
Physical Location & Seq
Forward (+)
18795926 .. 18797263
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45630.1

Sequence Viewer

Length: 561 bp
ATGCAAAACAGATCAATCTCGCAAGTCGCAACGATGGCGACGAGCTCTTCCACCGAGAAACTGCCACGTGTCCTCATCGTCAAACGTCCCGACGCCATGGCTGTGTTAGGCGACGGATTCGTCGCCTCCACCAAGTTCGAGATCCTCAAAGCCTTCGAGTCTCCGCTTCCTCTTCCTGAATTCTTGGCCTATCACTCTGACTCCATCTCCGCCATTATAGCCCCTGTAGCTGCTCCGGTCACAGCCGATCTGATTCGCATCCTCCCTAATCTTCGCTTGGTTGTTACTACCAGCGCCGGCGTTGACCATGTTGACTTAGTTGAGTGTCGTCGTCGAGGCATCTCCGTCGCCAACGCTGGCTCAAGCTTCTCGGAAGATGTCGCAGATACTGCCGTCGGTTTACTCATCGACGTCTTCCGCCGCATCTCCGCCGCCAATCGGTTCGTTAAGCAACGGTTTTGGCCACTCAAAGGCGATTATCCTCTCGGTTCCAAGGTTTGCAACATAACTTGTGAAAAAATCAAATTAGCAACAAAAATGATTGTTAATGGTTCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

186

Amino Acids

20.09

Weight (kDa)

9.3

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 66 - 151 4e-16 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 414
AciI CCGC 6 cut(s) 164, 210, 418, 421, 429, 432
AclWI GGATC 1 cut(s) 136
AcoI YGGCCR 1 cut(s) 461
AcsI RAATTY 1 cut(s) 179
AcvI CACGTG 1 cut(s) 68
AcyI GRCGYC 2 cut(s) 93, 411
AfiI CCNNNNNNNGG 2 cut(s) 438, 470
AflIII ACRYGT 1 cut(s) 67
AluBI AGCT 3 cut(s) 45, 230, 366
AluI AGCT 3 cut(s) 45, 230, 366
Alw21I GWGCWC 1 cut(s) 47
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 1 cut(s) 136
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 2 cut(s) 186, 461
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 179
AspLEI GCGC 1 cut(s) 296
BaeI ACNNNNGTAYC 2 cut(s) 378, 411
BalI TGGCCA 1 cut(s) 463
BanII GRGCYC 1 cut(s) 47
BbrPI CACGTG 1 cut(s) 68
BbsI GAAGAC 1 cut(s) 406
Bbv12I GWGCWC 1 cut(s) 47
BbvI GCAGC 1 cut(s) 217
BccI CCATC 2 cut(s) 28, 212
BceAI ACGGC 1 cut(s) 377
BcgI CGANNNNNNTGC 2 cut(s) 328, 362
BcoDI GTCTC 1 cut(s) 165
BfmI CTRYAG 1 cut(s) 225
BfoI RGCGCY 1 cut(s) 297
BisI GCNGC 3 cut(s) 231, 421, 432
BlsI GCNGC 3 cut(s) 232, 422, 433
BmiI GGNNCC 2 cut(s) 490, 553
BmsI GCATC 3 cut(s) 267, 348, 432
BpiI GAAGAC 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 68
BsaBI GATNNNNATC 1 cut(s) 257
BsaHI GRCGYC 2 cut(s) 93, 411
BsaJI CCNNGG 2 cut(s) 96, 492
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 4 cut(s) 185, 191, 215, 221
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 470
Bse118I RCCGGY 1 cut(s) 296
Bse8I GATNNNNATC 1 cut(s) 257
BseDI CCNNGG 2 cut(s) 96, 492
BseGI GGATG 1 cut(s) 258
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 2 cut(s) 438, 470
BseXI GCAGC 1 cut(s) 217
BshFI GGCC 2 cut(s) 188, 463
BsiHKAI GWGCWC 1 cut(s) 47
BsiSI CCGG 2 cut(s) 236, 297
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 2 cut(s) 438, 470
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 2 cut(s) 188, 463
Bsp1286I GDGCHC 1 cut(s) 47
Bsp143I GATC 3 cut(s) 11, 141, 247
Bsp19I CCATGG 1 cut(s) 96
BspACI CCGC 6 cut(s) 164, 210, 418, 421, 429, 432
BspANI GGCC 2 cut(s) 188, 463
BspLI GGNNCC 2 cut(s) 490, 553
BspPI GGATC 1 cut(s) 136
BspQI GCTCTTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 296
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 2 cut(s) 96, 492
BssMI GATC 3 cut(s) 11, 141, 247
BssNI GRCGYC 2 cut(s) 93, 411
BssT1I CCWWGG 2 cut(s) 96, 492
Bst4CI ACNGT 1 cut(s) 456
Bst6I CTCTTC 2 cut(s) 52, 177
BstACI GRCGYC 2 cut(s) 93, 411
BstAPI GCANNNNNTGC 1 cut(s) 389
BstBAI YACGTR 1 cut(s) 68
BstC8I GCNNGC 2 cut(s) 298, 358
BstDEI CTNAG 1 cut(s) 316
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 297
BstHHI GCGC 1 cut(s) 296
BstKTI GATC 3 cut(s) 14, 144, 250
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 3 cut(s) 11, 141, 247
BstMWI GCNNNNNNNGC 4 cut(s) 35, 218, 227, 389
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 1 cut(s) 217
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 141
BstYI RGATCY 1 cut(s) 141
BsuRI GGCC 2 cut(s) 188, 463
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 1 cut(s) 258
Cac8I GCNNGC 2 cut(s) 298, 358
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 1 cut(s) 296
Cfr10I RCCGGY 1 cut(s) 296
CseI GACGC 1 cut(s) 101
CviAII CATG 2 cut(s) 97, 308
DdeI CTNAG 1 cut(s) 316
DpnI GATC 3 cut(s) 13, 143, 249
DpnII GATC 3 cut(s) 11, 141, 247
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 461
Eam1104I CTCTTC 2 cut(s) 52, 177
EarI CTCTTC 2 cut(s) 52, 177
EciI GGCGGA 3 cut(s) 199, 407, 418
Ecl136II GAGCTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 96, 492
Eco24I GRGCYC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 45
Eco72I CACGTG 1 cut(s) 68
EcoICRI GAGCTC 1 cut(s) 45
EcoRI GAATTC 1 cut(s) 179
EcoT14I CCWWGG 2 cut(s) 96, 492
EcoT38I GRGCYC 1 cut(s) 47
ErhI CCWWGG 2 cut(s) 96, 492
FaeI CATG 2 cut(s) 100, 311
FaiI YATR 4 cut(s) 98, 218, 309, 506
FaqI GGGAC 1 cut(s) 72
FatI CATG 2 cut(s) 96, 307
Fnu4HI GCNGC 3 cut(s) 231, 421, 432
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 1 cut(s) 47
Fsp4HI GCNGC 3 cut(s) 231, 421, 432
GlaI GCGC 1 cut(s) 295
GluI GCNGC 3 cut(s) 231, 421, 432
HaeII RGCGCY 1 cut(s) 297
HaeIII GGCC 2 cut(s) 188, 463
HapII CCGG 2 cut(s) 236, 297
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 296
Hin1I GRCGYC 2 cut(s) 93, 411
Hin1II CATG 2 cut(s) 100, 311
Hin6I GCGC 1 cut(s) 294
HinP1I GCGC 1 cut(s) 294
HincII GTYRAC 2 cut(s) 304, 313
HindII GTYRAC 2 cut(s) 304, 313
HindIII AAGCTT 1 cut(s) 364
HinfI GANTC 4 cut(s) 117, 158, 200, 253
HpaII CCGG 2 cut(s) 236, 297
Hpy166II GTNNAC 3 cut(s) 304, 313, 401
Hpy188I TCNGA 3 cut(s) 199, 252, 373
Hpy188III TCNNGA 3 cut(s) 89, 139, 176
Hpy8I GTNNAC 3 cut(s) 304, 313, 401
Hpy99I CGWCG 9 cut(s) 43, 95, 116, 125, 333, 336, 350, 398, 413
HpyAV CCTTC 1 cut(s) 163
HpyCH4III ACNGT 1 cut(s) 456
HpyCH4IV ACGT 3 cut(s) 67, 85, 411
HpyCH4V TGCA 2 cut(s) 4, 501
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 218, 227, 389
HpyF3I CTNAG 1 cut(s) 316
HpySE526I ACGT 3 cut(s) 67, 85, 411
Hsp92I GRCGYC 2 cut(s) 93, 411
Hsp92II CATG 2 cut(s) 100, 311
HspAI GCGC 1 cut(s) 294
KroI GCCGGC 1 cut(s) 296
KroNI GCCGGC 1 cut(s) 298
Kzo9I GATC 3 cut(s) 11, 141, 247
LguI GCTCTTC 1 cut(s) 52
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 6 cut(s) 189, 237, 249, 304, 310, 342
Lsp1109I GCAGC 1 cut(s) 217
LweI GCATC 3 cut(s) 267, 348, 432
MaeII ACGT 3 cut(s) 67, 85, 411
MaeIII GTNAC 2 cut(s) 238, 283
MalI GATC 3 cut(s) 13, 143, 249
MboI GATC 3 cut(s) 11, 141, 247
MboII GAAGA 5 cut(s) 39, 164, 263, 386, 406
MflI RGATCY 1 cut(s) 141
MhlI GDGCHC 1 cut(s) 47
MlsI TGGCCA 1 cut(s) 463
MluCI AATT 2 cut(s) 179, 524
MluNI TGGCCA 1 cut(s) 463
MlyI GAGTC 2 cut(s) 167, 194
MnlI CCTC 7 cut(s) 83, 136, 155, 180, 272, 329, 492
Mox20I TGGCCA 1 cut(s) 463
MreI CGCCGGCG 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 296
MscI TGGCCA 1 cut(s) 463
MseI TTAA 2 cut(s) 447, 546
MslI CAYNNNNRTG 1 cut(s) 101
Msp20I TGGCCA 1 cut(s) 463
MspI CCGG 2 cut(s) 236, 297
MwoI GCNNNNNNNGC 4 cut(s) 35, 218, 227, 389
NaeI GCCGGC 1 cut(s) 298
NcoI CCATGG 1 cut(s) 96
NdeII GATC 3 cut(s) 11, 141, 247
NgoMIV GCCGGC 1 cut(s) 296
NlaIII CATG 2 cut(s) 100, 311
NlaIV GGNNCC 2 cut(s) 490, 553
NmuCI GTSAC 1 cut(s) 238
PciSI GCTCTTC 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 38
PdiI GCCGGC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 117, 253
PkrI GCNGC 3 cut(s) 232, 422, 433
PleI GAGTC 2 cut(s) 166, 194
PmaCI CACGTG 1 cut(s) 68
PmlI CACGTG 1 cut(s) 68
PpsI GAGTC 2 cut(s) 166, 194
Ppu21I YACGTR 1 cut(s) 68
Psp124BI GAGCTC 1 cut(s) 47
PspCI CACGTG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 490, 553
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 1 cut(s) 141
RseI CAYNNNNRTG 1 cut(s) 101
SacI GAGCTC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 52
SaqAI TTAA 2 cut(s) 447, 546
SatI GCNGC 3 cut(s) 231, 421, 432
Sau3AI GATC 3 cut(s) 11, 141, 247
SchI GAGTC 2 cut(s) 167, 194
SduI GDGCHC 1 cut(s) 47
SetI ASST 7 cut(s) 47, 70, 88, 232, 368, 414, 498
SfaNI GCATC 3 cut(s) 267, 348, 432
SfcI CTRYAG 1 cut(s) 225
SgrAI CRCCGGYG 1 cut(s) 296
SmiMI CAYNNNNRTG 1 cut(s) 101
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
Sse9I AATT 2 cut(s) 179, 524
SsiI CCGC 6 cut(s) 164, 210, 418, 421, 429, 432
SstI GAGCTC 1 cut(s) 47
StyI CCWWGG 2 cut(s) 96, 492
TaaI ACNGT 1 cut(s) 456
TaiI ACGT 3 cut(s) 70, 88, 414
TaqI TCGA 4 cut(s) 138, 156, 334, 408
TasI AATT 2 cut(s) 179, 524
TauI GCSGC 2 cut(s) 423, 434
TfiI GAWTC 2 cut(s) 117, 253
Tru1I TTAA 2 cut(s) 447, 546
Tru9I TTAA 2 cut(s) 447, 546
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 238
TspGWI ACGGA 2 cut(s) 129, 334
XapI RAATTY 1 cut(s) 179
ZraI GACGTC 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.