RLG00000028406

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22389494 .. 22392980
3487 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028406

Sequence Viewer

Length: 1101 bp
ATGGCAGATGATGATCACAGTGAGCTCCCTACAGTTCTTGTACTTCGCCGGTGCCCATTCATTGCTCTCTTGGAACCCAAATGCTCCCAGAAGTTCAACTTACTAAAAGCCTGGGACTCTCCTCTCCCTCAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCGATCCTCTCCTCCGGCAATGGGCCTCCCATCACAGCCCAAATCCTCCAGTTGCTCCCCTCCCTCAAACTCGTAGTCACCAACAGCACCGGCCTTGACCACGTTGACTTGGCCGAATGTCGACGCCGCGGCATTGCGGTCGCCAACGCCGCTAAAGTGTTTGCTGAGGACGTTGCTGACACGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCGGTATGTTAGGGGTGGACTGTGGGCTTGCAGAGGCGACTATCCTCTTGGTTCTAAGTCTTCTTTGTTGTCTACATGCTCACCCTCCGACGTTAAAAGATCCATTCCTCCTCACTTCATAGCCATCATCAGATCTATAGCCGCCGACGATGAATACTTTATTCTCAAGGAGGATGCCTATTGGAGGAAAGAAGGTTGGTATTGTTGGATTGGGCAGCATTGGGTTAGAAGTTGCAAAGAGATTGGAGGGATTTGGTGCAGTGTCTCGTACAATTCGAGGAGCGAAAAGCCGTCTTTGTCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATCATCTGTTGTGGACTGACAGAGGAAACCCACCACATGATCAACAAGGAAGTTTTGTCTGCATTGGGAAGAGAGGGGGTGATCGTTAATGTAGGGCGCGGAGCTATTATCGATGAGAAGGAAATGGTCGAGTGTTTGGTGCGAGGAGAGATCAGAGGTGCTGGCTTGGATGTGTTCGAGAATGAGCCTAATGTTCCTAAAGAGCTCTTTTCATTGGATAATGTTGTACTGTCACCGCATCAAGCTATTGTTACACATGAAGCTTTTGTGGATTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAGCCGTTGCTTTCTCTGGCTGTGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

367

Amino Acids

40.36

Weight (kDa)

5.44

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 41 - 361 7.3e-20 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 203 - 332 2.3e-32 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 51
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 2 cut(s) 289, 470
AccII CGCG 2 cut(s) 297, 849
AciI CCGC 8 cut(s) 157, 295, 297, 305, 318, 540, 849, 986
AclWI GGATC 2 cut(s) 166, 492
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 1045
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 3 cut(s) 42, 668, 978
AfiI CCNNNNNNNGG 2 cut(s) 189, 582
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 110, 165
AleI CACNNNNGTG 1 cut(s) 353
AluBI AGCT 6 cut(s) 25, 727, 854, 955, 995, 1013
AluI AGCT 6 cut(s) 25, 727, 854, 955, 995, 1013
Alw21I GWGCWC 2 cut(s) 27, 957
Alw26I GTCTC 1 cut(s) 667
AlwI GGATC 2 cut(s) 166, 492
AoxI GGCC 3 cut(s) 191, 259, 279
ApeKI GCWGC 2 cut(s) 613, 731
ApoI RAATTY 1 cut(s) 1045
ArsI GACNNNNNNTTYG 2 cut(s) 228, 260
AseI ATTAAT 1 cut(s) 1095
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 1 cut(s) 191
AsuHPI GGTGA 5 cut(s) 238, 471, 841, 975, 1040
AsuII TTCGAA 1 cut(s) 395
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 51
BanII GRGCYC 2 cut(s) 27, 957
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 2 cut(s) 27, 957
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 2 cut(s) 625, 743
BccI CCATC 2 cut(s) 206, 530
BceAI ACGGC 3 cut(s) 366, 673, 1057
BcgI CGANNNNNNTGC 2 cut(s) 289, 323
BciT130I CCWGG 2 cut(s) 112, 167
BclI TGATCA 2 cut(s) 13, 789
BcoDI GTCTC 1 cut(s) 667
BfmI CTRYAG 2 cut(s) 30, 534
BglII AGATCT 1 cut(s) 530
BisI GCNGC 6 cut(s) 295, 298, 318, 540, 614, 732
BlsI GCNGC 6 cut(s) 296, 299, 319, 541, 615, 733
Bme1390I CCNGG 2 cut(s) 112, 167
BmgT120I GGNCC 1 cut(s) 191
BmiI GGNNCC 2 cut(s) 53, 75
BmrFI CCNGG 2 cut(s) 112, 167
BmsI GCATC 3 cut(s) 562, 733, 997
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 1 cut(s) 200
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 114, 548
Bsa29I ATCGAT 1 cut(s) 861
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 111, 295
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 189, 582
Bse118I RCCGGY 2 cut(s) 48, 257
Bse1I ACTGG 2 cut(s) 136, 217
Bse3DI GCAATG 3 cut(s) 60, 193, 300
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 112, 167
BseCI ATCGAT 1 cut(s) 861
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 2 cut(s) 577, 925
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 2 cut(s) 189, 582
BseMI GCAATG 3 cut(s) 60, 193, 300
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 136, 217
BseRI GAGGAG 5 cut(s) 111, 169, 498, 691, 909
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 625, 743
BsgI GTGCAG 1 cut(s) 676
Bsh1236I CGCG 2 cut(s) 297, 849
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 3 cut(s) 193, 261, 281
BshNI GGYRCC 1 cut(s) 51
BshVI ATCGAT 1 cut(s) 861
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 2 cut(s) 27, 957
BsiSI CCGG 3 cut(s) 49, 183, 258
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 2 cut(s) 189, 582
BsmAI GTCTC 1 cut(s) 667
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 1055
BsnI GGCC 3 cut(s) 193, 261, 281
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 27, 56, 957
Bsp143I GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
BspACI CCGC 8 cut(s) 157, 295, 297, 305, 318, 540, 849, 986
BspANI GGCC 3 cut(s) 193, 261, 281
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 861
BspFNI CGCG 2 cut(s) 297, 849
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 53, 75
BspPI GGATC 2 cut(s) 166, 492
BspT104I TTCGAA 1 cut(s) 395
BspT107I GGYRCC 1 cut(s) 51
BsrBI CCGCTC 1 cut(s) 159
BsrDI GCAATG 3 cut(s) 60, 193, 300
BsrFI RCCGGY 2 cut(s) 48, 257
BsrI ACTGG 2 cut(s) 136, 217
BssAI RCCGGY 2 cut(s) 48, 257
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
BssNI GRCGYC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 112, 167
Bst4CI ACNGT 5 cut(s) 20, 34, 420, 740, 981
Bst6I CTCTTC 1 cut(s) 814
BstACI GRCGYC 1 cut(s) 292
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 4 cut(s) 157, 427, 729, 913
BstDEI CTNAG 2 cut(s) 333, 453
BstDSI CCRYGG 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 580
BstF5I GGATG 2 cut(s) 577, 925
BstFNI CGCG 2 cut(s) 297, 849
BstHHI GCGC 1 cut(s) 849
BstKTI GATC 7 cut(s) 16, 174, 500, 533, 792, 834, 903
BstMAI GTCTC 1 cut(s) 667
BstMBI GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
BstMCI CGRYCG 1 cut(s) 309
BstMWI GCNNNNNNNGC 2 cut(s) 317, 350
BstNI CCWGG 2 cut(s) 112, 167
BstNSI RCATGY 1 cut(s) 477
BstSCI CCNGG 2 cut(s) 110, 165
BstSFI CTRYAG 2 cut(s) 30, 534
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 2 cut(s) 297, 849
BstV1I GCAGC 2 cut(s) 625, 743
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 2 cut(s) 497, 530
BstYI RGATCY 2 cut(s) 497, 530
Bsu15I ATCGAT 1 cut(s) 861
BsuRI GGCC 3 cut(s) 193, 261, 281
BsuTUI ATCGAT 1 cut(s) 861
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 577, 925
BtsI GCAGTG 1 cut(s) 664
BtsIMutI CAGTG 3 cut(s) 25, 664, 745
Cac8I GCNNGC 4 cut(s) 157, 427, 729, 913
CciI TCATGA 1 cut(s) 375
CfoI GCGC 1 cut(s) 849
Cfr10I RCCGGY 2 cut(s) 48, 257
Cfr13I GGNCC 1 cut(s) 191
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 861
CseI GACGC 1 cut(s) 300
Csp6I GTAC 3 cut(s) 41, 667, 977
CviAII CATG 4 cut(s) 376, 474, 787, 1007
CviQI GTAC 3 cut(s) 41, 667, 977
DdeI CTNAG 2 cut(s) 333, 453
DpnI GATC 7 cut(s) 15, 173, 499, 532, 791, 833, 902
DpnII GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 814
EarI CTCTTC 1 cut(s) 814
Ecl136II GAGCTC 2 cut(s) 25, 955
Eco24I GRGCYC 2 cut(s) 27, 957
Eco53kI GAGCTC 2 cut(s) 25, 955
EcoICRI GAGCTC 2 cut(s) 25, 955
EcoNI CCTNNNNNAGG 1 cut(s) 580
EcoRII CCWGG 2 cut(s) 110, 165
EcoT38I GRGCYC 2 cut(s) 27, 957
FaeI CATG 4 cut(s) 379, 477, 790, 1010
FaiI YATR 8 cut(s) 377, 405, 475, 518, 536, 700, 788, 1008
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 375, 473, 786, 1006
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 2 cut(s) 13, 789
FblI GTMKAC 2 cut(s) 289, 470
Fnu4HI GCNGC 6 cut(s) 295, 298, 318, 540, 614, 732
FokI GGATG 2 cut(s) 584, 932
FriOI GRGCYC 2 cut(s) 27, 957
Fsp4HI GCNGC 6 cut(s) 295, 298, 318, 540, 614, 732
GlaI GCGC 1 cut(s) 848
GluI GCNGC 6 cut(s) 295, 298, 318, 540, 614, 732
GsuI CTGGAG 1 cut(s) 200
HaeIII GGCC 3 cut(s) 193, 261, 281
HapII CCGG 3 cut(s) 49, 183, 258
HgaI GACGC 1 cut(s) 300
HhaI GCGC 1 cut(s) 849
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 4 cut(s) 379, 477, 790, 1010
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HincII GTYRAC 2 cut(s) 274, 290
HindII GTYRAC 2 cut(s) 274, 290
HindIII AAGCTT 1 cut(s) 1011
HinfI GANTC 2 cut(s) 116, 397
HpaII CCGG 3 cut(s) 49, 183, 258
HphI GGTGA 5 cut(s) 238, 471, 841, 975, 1040
Hpy166II GTNNAC 5 cut(s) 274, 290, 416, 471, 764
Hpy188I TCNGA 3 cut(s) 487, 530, 905
Hpy188III TCNNGA 4 cut(s) 131, 142, 376, 928
Hpy8I GTNNAC 5 cut(s) 274, 290, 416, 471, 764
Hpy99I CGWCG 3 cut(s) 294, 491, 548
HpyAV CCTTC 2 cut(s) 584, 862
HpyCH4III ACNGT 5 cut(s) 20, 34, 420, 740, 981
HpyCH4IV ACGT 3 cut(s) 270, 339, 489
HpyCH4V TGCA 5 cut(s) 429, 633, 657, 731, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 317, 350
HpyF3I CTNAG 2 cut(s) 333, 453
HpySE526I ACGT 3 cut(s) 270, 339, 489
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 4 cut(s) 379, 477, 790, 1010
HspAI GCGC 1 cut(s) 847
Ksp22I TGATCA 2 cut(s) 13, 789
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
LmnI GCTCC 6 cut(s) 30, 89, 164, 228, 678, 851
Lsp1109I GCAGC 2 cut(s) 625, 743
LweI GCATC 3 cut(s) 562, 733, 997
MaeII ACGT 3 cut(s) 270, 339, 489
MaeIII GTNAC 4 cut(s) 244, 981, 1000, 1036
MalI GATC 7 cut(s) 15, 173, 499, 532, 791, 833, 902
MbiI CCGCTC 1 cut(s) 159
MboI GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
MboII GAAGA 3 cut(s) 450, 831, 1051
MflI RGATCY 2 cut(s) 497, 530
MhlI GDGCHC 3 cut(s) 27, 56, 957
MluCI AATT 5 cut(s) 670, 704, 1031, 1045, 1096
MlyI GAGTC 1 cut(s) 110
MmeI TCCRAC 2 cut(s) 510, 584
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 3 cut(s) 492, 837, 1095
MslI CAYNNNNRTG 1 cut(s) 353
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 3 cut(s) 49, 183, 258
MspR9I CCNGG 2 cut(s) 112, 167
Mva1269I GAATGC 1 cut(s) 1055
MvaI CCWGG 2 cut(s) 112, 167
MvnI CGCG 2 cut(s) 297, 849
MwoI GCNNNNNNNGC 2 cut(s) 317, 350
NdeII GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
NlaIII CATG 4 cut(s) 379, 477, 790, 1010
NlaIV GGNNCC 2 cut(s) 53, 75
NmuCI GTSAC 2 cut(s) 244, 981
NspI RCATGY 1 cut(s) 477
NspV TTCGAA 1 cut(s) 395
OliI CACNNNNGTG 1 cut(s) 353
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 671
PctI GAATGC 1 cut(s) 1055
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 6 cut(s) 296, 299, 319, 541, 615, 733
PleI GAGTC 1 cut(s) 110
PpsI GAGTC 1 cut(s) 110
PshBI ATTAAT 1 cut(s) 1095
Psp124BI GAGCTC 2 cut(s) 27, 957
Psp6I CCWGG 2 cut(s) 110, 165
PspGI CCWGG 2 cut(s) 110, 165
PspN4I GGNNCC 2 cut(s) 53, 75
PspPI GGNCC 1 cut(s) 191
PsuI RGATCY 2 cut(s) 497, 530
RsaI GTAC 3 cut(s) 42, 668, 978
RsaNI GTAC 3 cut(s) 41, 667, 977
RseI CAYNNNNRTG 1 cut(s) 353
SacI GAGCTC 2 cut(s) 27, 957
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 3 cut(s) 492, 837, 1095
SatI GCNGC 6 cut(s) 295, 298, 318, 540, 614, 732
Sau3AI GATC 7 cut(s) 13, 171, 497, 530, 789, 831, 900
Sau96I GGNCC 1 cut(s) 191
SchI GAGTC 1 cut(s) 110
ScrFI CCNGG 2 cut(s) 112, 167
SduI GDGCHC 3 cut(s) 27, 56, 957
SfaNI GCATC 3 cut(s) 562, 733, 997
SfcI CTRYAG 2 cut(s) 30, 534
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 395
SgrAI CRCCGGYG 1 cut(s) 48
SgrBI CCGCGG 1 cut(s) 298
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 2 cut(s) 129, 563
SmoI CTYRAG 2 cut(s) 129, 563
Sse9I AATT 5 cut(s) 670, 704, 1031, 1045, 1096
SsiI CCGC 8 cut(s) 157, 295, 297, 305, 318, 540, 849, 986
SstI GAGCTC 2 cut(s) 27, 957
StyD4I CCNGG 2 cut(s) 110, 165
TaaI ACNGT 5 cut(s) 20, 34, 420, 740, 981
TaiI ACGT 3 cut(s) 273, 342, 492
TaqI TCGA 6 cut(s) 289, 395, 674, 861, 879, 927
TasI AATT 5 cut(s) 670, 704, 1031, 1045, 1096
TatI WGTACW 2 cut(s) 40, 976
TauI GCSGC 4 cut(s) 297, 300, 320, 542
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 3 cut(s) 492, 837, 1095
Tru9I TTAA 3 cut(s) 492, 837, 1095
TscAI CASTG 3 cut(s) 25, 664, 745
TseFI GTSAC 2 cut(s) 244, 981
TseI GCWGC 2 cut(s) 613, 731
Tsp45I GTSAC 2 cut(s) 244, 981
TspDTI ATGAA 6 cut(s) 49, 355, 505, 564, 951, 1023
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 3 cut(s) 25, 664, 745
VspI ATTAAT 1 cut(s) 1095
XagI CCTNNNNNAGG 1 cut(s) 580
XapI RAATTY 1 cut(s) 1045
XceI RCATGY 1 cut(s) 477
XmiI GTMKAC 2 cut(s) 289, 470
XmnI GAANNNNTTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.