Rroxscaffold_4G00303730

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000446
Physical Location & Seq
Reverse (-)
24117396 .. 24119906
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303730.1

Sequence Viewer

Length: 975 bp
ATGGCCCTATTGCCAAACCTCCCAGATGTCCTGATCATCCAACCACCACCAGCCTTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGAGCATGGGAATCACCACTCCCTCTGGACCAGTTCCTCACCACCCACGCTCAATCAGTCCAGGCCCTCCTCTGCCGCGCCGAGACTCCGATCAACACCGACGTCCTCCGGCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCCGACCAAGTGGACAAAGCCGAGTGCCGGCGGCGTGGGATTGCGGTGACCAACGCAGGAAATGCGTACGCGGAAGATGTCGCTGATATCGCTGTTGGGTTGTTCATCGATGTGTTTCGGAAGATTTCAGCAGCTGATTGGTATGTGAGAAAAGGGCTTTGGAGTAGCAAAGGAGATTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGAAGCGAAAGGCCGTCTTTGTCATACACCTTCTATTCCAATGTCAGTGAACTTGCAGCAGACAGGTGTCAAAAGTTCACTTCGTCGGATCTCAGGTGTGCAGTAGTACGGTCGACGGGCTACCCTGGTCGGTCAGAATATGGTCAGATTGAAGATCAAGGTTTGATGGTGACAAAGGCTACTGGAAGAGATCGTTGCGGCAGCTGTGGAGAGACAACGTGGTCGACGGAGCAAGCTTTTGGTGGGAGGTTGTGCTGTTCAAGCTTGGGCCATTTGAAAATTGTCTGTGTCATGGCTAGGACAGTTCGTGGATTGGGTTCTCTCAAACCTAGTGCTTGGGCTTTAGATCTGACCTATATTGGGTTGGATTTTGTGCTTGGGCTGCTCACTCATCACTTGGTTTATTTGGTTTATAGGCGTAGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

324

Amino Acids

35.45

Weight (kDa)

8.96

Isoelectric Point (pI)

37.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 211 9.7e-18 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 189 7.2e-15 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 766
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 2 cut(s) 659, 770
AccII CGCG 2 cut(s) 177, 323
AciI CCGC 6 cut(s) 175, 240, 283, 296, 323, 744
AclWI GGATC 1 cut(s) 642
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 2 cut(s) 320, 654
AfiI CCNNNNNNNGG 3 cut(s) 106, 279, 906
AgsI TTSAA 4 cut(s) 91, 698, 807, 823
AjnI CCWGG 2 cut(s) 159, 670
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 4 cut(s) 386, 750, 782, 810
AluI AGCT 4 cut(s) 386, 750, 782, 810
Alw26I GTCTC 2 cut(s) 176, 752
AlwI GGATC 1 cut(s) 642
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 6 cut(s) 3, 162, 219, 516, 557, 814
ApeKI GCWGC 6 cut(s) 211, 383, 525, 602, 747, 928
AspLEI GCGC 2 cut(s) 179, 254
AspS9I GGNCC 5 cut(s) 4, 127, 163, 220, 814
AsuHPI GGTGA 5 cut(s) 70, 105, 130, 310, 727
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BbvI GCAGC 6 cut(s) 198, 395, 512, 614, 759, 915
BccI CCATC 1 cut(s) 706
BceAI ACGGC 1 cut(s) 544
BciT130I CCWGG 2 cut(s) 161, 672
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 2 cut(s) 176, 752
BfaI CTAG 2 cut(s) 843, 876
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 2 cut(s) 531, 892
BisI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 745, 748, 929
BlsI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 746, 749, 930
Bme1390I CCNGG 2 cut(s) 161, 672
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 5 cut(s) 4, 127, 163, 220, 814
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 2 cut(s) 161, 672
BoxI GACNNNNGTC 1 cut(s) 612
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaJI CCNNGG 1 cut(s) 670
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 279, 906
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 733
BseBI CCWGG 2 cut(s) 161, 672
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 2 cut(s) 36, 93
BseLI CCNNNNNNNGG 3 cut(s) 106, 279, 906
BseMII CTCAG 1 cut(s) 652
BseNI ACTGG 2 cut(s) 130, 733
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 198, 395, 512, 614, 759, 915
BsgI GTGCAG 1 cut(s) 666
Bsh1236I CGCG 2 cut(s) 177, 323
Bsh1285I CGRYCG 1 cut(s) 659
BshFI GGCC 6 cut(s) 5, 164, 221, 518, 559, 816
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiEI CGRYCG 1 cut(s) 659
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 4 cut(s) 208, 218, 249, 280
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 3 cut(s) 106, 279, 906
BsmAI GTCTC 2 cut(s) 176, 752
BsnI GGCC 6 cut(s) 5, 164, 221, 518, 559, 816
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
BspACI CCGC 6 cut(s) 175, 240, 283, 296, 323, 744
BspANI GGCC 6 cut(s) 5, 164, 221, 518, 559, 816
BspCNI CTCAG 1 cut(s) 651
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 2 cut(s) 177, 323
BspLI GGNNCC 2 cut(s) 253, 442
BspPI GGATC 1 cut(s) 642
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 733
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
BssNI GRCGYC 2 cut(s) 201, 252
Bst2UI CCWGG 2 cut(s) 161, 672
Bst4CI ACNGT 2 cut(s) 657, 850
Bst6I CTCTTC 1 cut(s) 727
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 3 cut(s) 244, 281, 780
BstDEI CTNAG 1 cut(s) 638
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 2 cut(s) 36, 93
BstFNI CGCG 2 cut(s) 177, 323
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 179, 254
BstKTI GATC 8 cut(s) 36, 192, 231, 534, 637, 703, 739, 895
BstMAI GTCTC 2 cut(s) 176, 752
BstMBI GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
BstMCI CGRYCG 1 cut(s) 659
BstMWI GCNNNNNNNGC 4 cut(s) 314, 341, 807, 928
BstNI CCWGG 2 cut(s) 161, 672
BstPAI GACNNNNGTC 1 cut(s) 612
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 2 cut(s) 159, 670
BstUI CGCG 2 cut(s) 177, 323
BstV1I GCAGC 6 cut(s) 198, 395, 512, 614, 759, 915
BstX2I RGATCY 3 cut(s) 531, 634, 892
BstYI RGATCY 3 cut(s) 531, 634, 892
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 6 cut(s) 5, 164, 221, 518, 559, 816
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 2 cut(s) 36, 93
BtsIMutI CAGTG 1 cut(s) 598
Cac8I GCNNGC 3 cut(s) 244, 281, 780
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 2 cut(s) 179, 254
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 5 cut(s) 4, 127, 163, 220, 814
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 2 cut(s) 319, 653
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 2 cut(s) 105, 838
CviQI GTAC 2 cut(s) 319, 653
DdeI CTNAG 1 cut(s) 638
DinI GGCGCC 1 cut(s) 253
DpnI GATC 8 cut(s) 35, 191, 230, 533, 636, 702, 738, 894
DpnII GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
DrdI GACNNNNNNGTC 1 cut(s) 766
DseDI GACNNNNNNGTC 1 cut(s) 766
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
EciI GGCGGA 1 cut(s) 229
Eco147I AGGCCT 1 cut(s) 518
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 298
EcoO109I RGGNCCY 1 cut(s) 163
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 2 cut(s) 159, 670
EcoRV GATATC 1 cut(s) 340
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
FaeI CATG 2 cut(s) 108, 841
FalI AAGNNNNNCTT 2 cut(s) 547, 579
FatI CATG 2 cut(s) 104, 837
FbaI TGATCA 1 cut(s) 33
FblI GTMKAC 2 cut(s) 659, 770
Fnu4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 745, 748, 929
FokI GGATG 2 cut(s) 23, 80
Fsp4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 745, 748, 929
FspBI CTAG 2 cut(s) 843, 876
GlaI GCGC 2 cut(s) 178, 253
GluI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 745, 748, 929
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 6 cut(s) 5, 164, 221, 518, 559, 816
HapII CCGG 4 cut(s) 208, 218, 249, 280
HhaI GCGC 2 cut(s) 179, 254
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 2 cut(s) 108, 841
Hin6I GCGC 2 cut(s) 177, 252
HinP1I GCGC 2 cut(s) 177, 252
HincII GTYRAC 2 cut(s) 660, 771
HindII GTYRAC 2 cut(s) 660, 771
HindIII AAGCTT 2 cut(s) 780, 808
HinfI GANTC 4 cut(s) 68, 110, 184, 510
HpaII CCGG 4 cut(s) 208, 218, 249, 280
HphI GGTGA 5 cut(s) 70, 105, 130, 310, 727
Hpy166II GTNNAC 5 cut(s) 265, 596, 624, 660, 771
Hpy188I TCNGA 8 cut(s) 73, 101, 189, 372, 634, 682, 693, 897
Hpy188III TCNNGA 3 cut(s) 31, 125, 546
Hpy8I GTNNAC 5 cut(s) 265, 596, 624, 660, 771
Hpy99I CGWCG 4 cut(s) 203, 634, 664, 775
HpyAV CCTTC 2 cut(s) 64, 586
HpyCH4III ACNGT 2 cut(s) 657, 850
HpyCH4IV ACGT 2 cut(s) 201, 764
HpyCH4V TGCA 3 cut(s) 528, 602, 647
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 341, 807, 928
HpyF3I CTNAG 1 cut(s) 638
HpySE526I ACGT 2 cut(s) 201, 764
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 2 cut(s) 108, 841
HspAI GCGC 2 cut(s) 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 1 cut(s) 33
Kzo9I GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
LmnI GCTCC 1 cut(s) 775
Lsp1109I GCAGC 6 cut(s) 198, 395, 512, 614, 759, 915
MaeI CTAG 2 cut(s) 843, 876
MaeII ACGT 2 cut(s) 201, 764
MaeIII GTNAC 2 cut(s) 298, 715
MalI GATC 8 cut(s) 35, 191, 230, 533, 636, 702, 738, 894
MboI GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
MboII GAAGA 5 cut(s) 238, 338, 385, 710, 744
MflI RGATCY 3 cut(s) 531, 634, 892
MluCI AATT 1 cut(s) 825
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 178, 504
MmeI TCCRAC 5 cut(s) 64, 96, 454, 612, 891
MroNI GCCGGC 1 cut(s) 279
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 386, 750
MspI CCGG 4 cut(s) 208, 218, 249, 280
MspR9I CCNGG 2 cut(s) 161, 672
MvaI CCWGG 2 cut(s) 161, 672
MvnI CGCG 2 cut(s) 177, 323
MwoI GCNNNNNNNGC 4 cut(s) 314, 341, 807, 928
NaeI GCCGGC 1 cut(s) 281
NarI GGCGCC 1 cut(s) 252
NdeII GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 2 cut(s) 108, 841
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 2 cut(s) 298, 715
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 68, 110
Pfl23II CGTACG 1 cut(s) 318
PkrI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 746, 749, 930
PleI GAGTC 2 cut(s) 178, 504
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 2 cut(s) 178, 504
PshAI GACNNNNGTC 1 cut(s) 612
Psp6I CCWGG 2 cut(s) 159, 670
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 2 cut(s) 159, 670
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 5 cut(s) 4, 127, 163, 220, 814
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 3 cut(s) 531, 634, 892
PvuII CAGCTG 2 cut(s) 386, 750
RsaI GTAC 2 cut(s) 320, 654
RsaNI GTAC 2 cut(s) 319, 653
RseI CAYNNNNRTG 1 cut(s) 362
SalI GTCGAC 2 cut(s) 658, 769
SatI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 745, 748, 929
Sau3AI GATC 8 cut(s) 33, 189, 228, 531, 634, 700, 736, 892
Sau96I GGNCC 5 cut(s) 4, 127, 163, 220, 814
SchI GAGTC 2 cut(s) 178, 504
ScrFI CCNGG 2 cut(s) 161, 672
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 1 cut(s) 825
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 6 cut(s) 175, 240, 283, 296, 323, 744
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 843, 876
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 2 cut(s) 159, 670
TaaI ACNGT 2 cut(s) 657, 850
TaiI ACGT 2 cut(s) 204, 767
TaqI TCGA 5 cut(s) 66, 360, 513, 659, 770
TaqII GACCGA 1 cut(s) 666
TasI AATT 1 cut(s) 825
TauI GCSGC 3 cut(s) 177, 286, 747
TfiI GAWTC 2 cut(s) 68, 110
TscAI CASTG 1 cut(s) 598
TseFI GTSAC 2 cut(s) 298, 715
TseI GCWGC 6 cut(s) 211, 383, 525, 602, 747, 928
Tsp45I GTSAC 2 cut(s) 298, 715
TspDTI ATGAA 3 cut(s) 46, 346, 510
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 1 cut(s) 598
VpaK11BI GGWCC 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 2 cut(s) 659, 770
XspI CTAG 2 cut(s) 843, 876
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.