Rh1CG218000

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000446
Physical Location & Seq
Forward (+)
46855988 .. 46866820
10833 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG218000.1

Sequence Viewer

Length: 1155 bp
ATGGCCCTATTGCCAAACCTCCCAGATGTCCTGATCATCCAACCACCACCAGCCTTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGCGCATGGGAGTCGCCACTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCATGCCCTCCTCTGCCGCGCCGAGACTCCGATCAACGCCGACGTCCTCCGGCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCCGACCAAGTGGACAAAGCCGAGTGCCGGCGGCGTGGGATTGCGGTGACCAACGCAGGAAATGCGTACGCGGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGATGTGTTTCGGAAGATCTCAGCAGCTGATTGCTATGTGAGAAAAGGGCTTTGGAGTAGCAGTGGAGATTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGTATCATTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCCTTTGGCTGCAAGATCTCATACAACTCAAGAAGCGAAAGGCCGGCTTTGTCATACACCTTCTATTCCAATGTCAGTGAACTTGCAGCAGACAGTGACGCTCTCGTAATCTGTTGTGGGTTAACAGACCAGACCCGCCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGGTAGTTAATATAGGGCGTGGGGCAATTATTGATGAGAAGGAATTGGTCAAGTGTATGGTGAATGGAGAGATTAGAGGTGCTGGTTTGGATGTGTATGAGAATGAGCCTCATATACCGAAAGAGCTCGTGGCAATGGACAACGTTGTTCTGTCTCCACATAAGGCTGTTTATACAGTAGAGTCTGTCAAGAAAGGAGATTTCAGGTGTGCAGCAGTAGGGTCATCGTGCTACCCAGGTCGCTCAGAATATGGTCAGATTGAAGACCAAGGTTGGATGGTGACAAAGGCTACTGGAAGAGATCGTCGCGGCTGCTGTGGAGAGACAACGTGGTCGACGGAGCAAGCTTTTTGGTGGGATGTTGTGCTGTTCAAGCTTGGGCCATTTGAAAATTGTCTGTGTCGGCTAGGACAGTTCGTGGATTGGGTTCTCTTAAACCTAGTGCTTGGGCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

384

Amino Acids

41.97

Weight (kDa)

5.78

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 229 3.8e-19 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 2.1e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1030
AatII GACGTC 1 cut(s) 204
AccB1I GGYRCC 1 cut(s) 251
AccI GTMKAC 1 cut(s) 1034
AccII CGCG 4 cut(s) 102, 177, 323, 1008
AciI CCGC 8 cut(s) 100, 175, 240, 283, 296, 323, 652, 1008
AclI AACGTT 1 cut(s) 843
AcuI CTGAAG 1 cut(s) 245
AcyI GRCGYC 2 cut(s) 201, 252
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 2 cut(s) 106, 279
AgsI TTSAA 4 cut(s) 91, 962, 1072, 1088
AjnI CCWGG 1 cut(s) 934
AjuI GAANNNNNNNTTGG 2 cut(s) 394, 426
AluBI AGCT 4 cut(s) 386, 826, 1046, 1075
AluI AGCT 4 cut(s) 386, 826, 1046, 1075
Alw21I GWGCWC 1 cut(s) 828
Alw26I GTCTC 3 cut(s) 176, 858, 1016
AlwNI CAGNNNCTG 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 5 cut(s) 3, 219, 516, 557, 1079
ApeKI GCWGC 6 cut(s) 211, 383, 525, 602, 911, 1011
AspLEI GCGC 3 cut(s) 104, 179, 254
AspS9I GGNCC 4 cut(s) 4, 127, 220, 1079
AsuHPI GGTGA 5 cut(s) 70, 130, 310, 772, 991
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 1 cut(s) 251
BanII GRGCYC 1 cut(s) 828
BauI CACGAG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 969
Bbv12I GWGCWC 1 cut(s) 828
BbvI GCAGC 6 cut(s) 198, 395, 512, 614, 923, 998
BccI CCATC 2 cut(s) 689, 970
BcgI CGANNNNNNTGC 2 cut(s) 93, 127
BciT130I CCWGG 1 cut(s) 936
BclI TGATCA 2 cut(s) 33, 660
BcoDI GTCTC 3 cut(s) 176, 858, 1016
BfaI CTAG 2 cut(s) 1106, 1139
BfoI RGCGCY 1 cut(s) 255
BglII AGATCT 2 cut(s) 375, 531
BisI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 912, 1009, 1012
BlsI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 913, 1010, 1013
Bme1390I CCNGG 1 cut(s) 936
Bme18I GGWCC 1 cut(s) 127
BmeT110I CYCGRG 1 cut(s) 512
BmgT120I GGNCC 4 cut(s) 4, 127, 220, 1079
BmiI GGNNCC 2 cut(s) 253, 442
BmrFI CCNGG 1 cut(s) 936
BpiI GAAGAC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
Bsa29I ATCGAT 1 cut(s) 360
BsaHI GRCGYC 2 cut(s) 201, 252
BsaJI CCNNGG 2 cut(s) 934, 967
BsaXI ACNNNNNCTCC 4 cut(s) 406, 436, 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 106, 279
Bse118I RCCGGY 4 cut(s) 217, 248, 279, 559
Bse1I ACTGG 2 cut(s) 130, 997
Bse3DI GCAATG 1 cut(s) 840
BseBI CCWGG 1 cut(s) 936
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 2 cut(s) 934, 967
BseGI GGATG 5 cut(s) 36, 93, 796, 981, 1063
BseLI CCNNNNNNNGG 2 cut(s) 106, 279
BseMI GCAATG 1 cut(s) 840
BseMII CTCAG 2 cut(s) 393, 957
BseNI ACTGG 2 cut(s) 130, 997
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 6 cut(s) 198, 395, 512, 614, 923, 998
BsgI GTGCAG 1 cut(s) 930
Bsh1236I CGCG 4 cut(s) 102, 177, 323, 1008
BshFI GGCC 5 cut(s) 5, 221, 518, 559, 1081
BshNI GGYRCC 1 cut(s) 251
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 828
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 5 cut(s) 208, 218, 249, 280, 560
BsiWI CGTACG 1 cut(s) 318
BslI CCNNNNNNNGG 2 cut(s) 106, 279
BsmAI GTCTC 3 cut(s) 176, 858, 1016
BsnI GGCC 5 cut(s) 5, 221, 518, 559, 1081
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 828
Bsp143I GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
BspACI CCGC 8 cut(s) 100, 175, 240, 283, 296, 323, 652, 1008
BspANI GGCC 5 cut(s) 5, 221, 518, 559, 1081
BspCNI CTCAG 2 cut(s) 392, 956
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 4 cut(s) 102, 177, 323, 1008
BspLI GGNNCC 2 cut(s) 253, 442
BspT104I TTCGAA 1 cut(s) 66
BspT107I GGYRCC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 840
BsrFI RCCGGY 4 cut(s) 217, 248, 279, 559
BsrI ACTGG 2 cut(s) 130, 997
BssAI RCCGGY 4 cut(s) 217, 248, 279, 559
BssECI CCNNGG 2 cut(s) 934, 967
BssMI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
BssNI GRCGYC 2 cut(s) 201, 252
BssSI CACGAG 1 cut(s) 827
BssT1I CCWWGG 1 cut(s) 967
Bst2BI CACGAG 1 cut(s) 827
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 3 cut(s) 611, 877, 1113
Bst6I CTCTTC 1 cut(s) 991
BstACI GRCGYC 2 cut(s) 201, 252
BstAPI GCANNNNNTGC 1 cut(s) 314
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 4 cut(s) 244, 281, 561, 1044
BstDEI CTNAG 2 cut(s) 379, 943
BstEII GGTNACC 1 cut(s) 298
BstF5I GGATG 5 cut(s) 36, 93, 796, 981, 1063
BstFNI CGCG 4 cut(s) 102, 177, 323, 1008
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 104, 179, 254
BstKTI GATC 7 cut(s) 36, 192, 231, 378, 534, 663, 1003
BstMAI GTCTC 3 cut(s) 176, 858, 1016
BstMBI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 314, 341, 1072
BstNI CCWGG 1 cut(s) 936
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 934
BstUI CGCG 4 cut(s) 102, 177, 323, 1008
BstV1I GCAGC 6 cut(s) 198, 395, 512, 614, 923, 998
BstV2I GAAGAC 1 cut(s) 969
BstX2I RGATCY 2 cut(s) 375, 531
BstYI RGATCY 2 cut(s) 375, 531
Bsu15I ATCGAT 1 cut(s) 360
BsuRI GGCC 5 cut(s) 5, 221, 518, 559, 1081
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 5 cut(s) 36, 93, 796, 981, 1063
BtsI GCAGTG 1 cut(s) 427
BtsIMutI CAGTG 3 cut(s) 427, 598, 616
Cac8I GCNNGC 4 cut(s) 244, 281, 561, 1044
CaiI CAGNNNCTG 1 cut(s) 386
CfoI GCGC 3 cut(s) 104, 179, 254
Cfr10I RCCGGY 4 cut(s) 217, 248, 279, 559
Cfr13I GGNCC 4 cut(s) 4, 127, 220, 1079
ClaI ATCGAT 1 cut(s) 360
CseI GACGC 1 cut(s) 623
Csp6I GTAC 1 cut(s) 319
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 161, 658
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 2 cut(s) 379, 943
DinI GGCGCC 1 cut(s) 253
DpnI GATC 7 cut(s) 35, 191, 230, 377, 533, 662, 1002
DpnII GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
DrdI GACNNNNNNGTC 1 cut(s) 1030
DseDI GACNNNNNNGTC 1 cut(s) 1030
Eam1104I CTCTTC 1 cut(s) 991
EarI CTCTTC 1 cut(s) 991
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 967
Eco147I AGGCCT 1 cut(s) 518
Eco24I GRGCYC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 340
Eco47I GGWCC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 826
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
Eco91I GGTNACC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 826
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 1 cut(s) 934
EcoRV GATATC 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 967
EcoT38I GRGCYC 1 cut(s) 828
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 967
FaeI CATG 3 cut(s) 108, 164, 661
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FatI CATG 3 cut(s) 104, 160, 657
FauI CCCGC 1 cut(s) 659
FbaI TGATCA 2 cut(s) 33, 660
FblI GTMKAC 1 cut(s) 1034
Fnu4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 912, 1009, 1012
FokI GGATG 5 cut(s) 23, 80, 803, 988, 1070
FriOI GRGCYC 1 cut(s) 828
Fsp4HI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 912, 1009, 1012
FspBI CTAG 2 cut(s) 1106, 1139
GlaI GCGC 3 cut(s) 103, 178, 253
GluI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 912, 1009, 1012
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 5 cut(s) 5, 221, 518, 559, 1081
HapII CCGG 5 cut(s) 208, 218, 249, 280, 560
HgaI GACGC 1 cut(s) 623
HhaI GCGC 3 cut(s) 104, 179, 254
Hin1I GRCGYC 2 cut(s) 201, 252
Hin1II CATG 3 cut(s) 108, 164, 661
Hin6I GCGC 3 cut(s) 102, 177, 252
HinP1I GCGC 3 cut(s) 102, 177, 252
HincII GTYRAC 2 cut(s) 639, 1035
HindII GTYRAC 2 cut(s) 639, 1035
HindIII AAGCTT 2 cut(s) 1044, 1073
HinfI GANTC 5 cut(s) 68, 110, 184, 510, 881
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 5 cut(s) 208, 218, 249, 280, 560
HphI GGTGA 5 cut(s) 70, 130, 310, 772, 991
Hpy166II GTNNAC 4 cut(s) 265, 596, 639, 1035
Hpy188I TCNGA 5 cut(s) 73, 189, 372, 946, 957
Hpy188III TCNNGA 4 cut(s) 31, 125, 546, 889
Hpy8I GTNNAC 4 cut(s) 265, 596, 639, 1035
Hpy99I CGWCG 3 cut(s) 203, 1008, 1039
HpyAV CCTTC 3 cut(s) 64, 586, 733
HpyCH4III ACNGT 3 cut(s) 611, 877, 1113
HpyCH4IV ACGT 3 cut(s) 201, 843, 1028
HpyCH4V TGCA 4 cut(s) 528, 602, 683, 911
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 341, 1072
HpyF3I CTNAG 2 cut(s) 379, 943
HpySE526I ACGT 3 cut(s) 201, 843, 1028
Hsp92I GRCGYC 2 cut(s) 201, 252
Hsp92II CATG 3 cut(s) 108, 164, 661
HspAI GCGC 3 cut(s) 102, 177, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 2 cut(s) 279, 559
KroNI GCCGGC 2 cut(s) 281, 561
Ksp22I TGATCA 2 cut(s) 33, 660
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
LmnI GCTCC 1 cut(s) 1039
Lsp1109I GCAGC 6 cut(s) 198, 395, 512, 614, 923, 998
MaeI CTAG 2 cut(s) 1106, 1139
MaeII ACGT 3 cut(s) 201, 843, 1028
MaeIII GTNAC 3 cut(s) 298, 611, 979
MalI GATC 7 cut(s) 35, 191, 230, 377, 533, 662, 1002
MboI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
MboII GAAGA 5 cut(s) 238, 338, 385, 974, 1008
MflI RGATCY 2 cut(s) 375, 531
MhlI GDGCHC 1 cut(s) 828
MluCI AATT 3 cut(s) 726, 743, 1090
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 4 cut(s) 119, 178, 504, 890
MmeI TCCRAC 3 cut(s) 64, 96, 953
MroNI GCCGGC 2 cut(s) 279, 559
MseI TTAA 3 cut(s) 638, 708, 1133
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 5 cut(s) 208, 218, 249, 280, 560
MspR9I CCNGG 1 cut(s) 936
MvaI CCWGG 1 cut(s) 936
MvnI CGCG 4 cut(s) 102, 177, 323, 1008
MwoI GCNNNNNNNGC 3 cut(s) 314, 341, 1072
NaeI GCCGGC 2 cut(s) 281, 561
NarI GGCGCC 1 cut(s) 252
NdeII GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
NgoMIV GCCGGC 2 cut(s) 279, 559
NlaIII CATG 3 cut(s) 108, 164, 661
NlaIV GGNNCC 2 cut(s) 253, 442
NmeAIII GCCGAG 2 cut(s) 205, 298
NmuCI GTSAC 3 cut(s) 298, 611, 979
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PceI AGGCCT 1 cut(s) 518
PdiI GCCGGC 2 cut(s) 281, 561
PfeI GAWTC 1 cut(s) 68
Pfl23II CGTACG 1 cut(s) 318
PkrI GCNGC 9 cut(s) 176, 213, 285, 385, 527, 604, 913, 1010, 1013
PleI GAGTC 4 cut(s) 118, 178, 504, 889
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 4 cut(s) 118, 178, 504, 889
Psp124BI GAGCTC 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 843
Psp6I CCWGG 1 cut(s) 934
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 1 cut(s) 934
PspLI CGTACG 1 cut(s) 318
PspN4I GGNNCC 2 cut(s) 253, 442
PspPI GGNCC 4 cut(s) 4, 127, 220, 1079
PspXI VCTCGAGB 1 cut(s) 512
PstNI CAGNNNCTG 1 cut(s) 386
PsuI RGATCY 2 cut(s) 375, 531
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 828
SalI GTCGAC 1 cut(s) 1033
SaqAI TTAA 3 cut(s) 638, 708, 1133
SatI GCNGC 9 cut(s) 175, 212, 284, 384, 526, 603, 912, 1009, 1012
Sau3AI GATC 7 cut(s) 33, 189, 228, 375, 531, 660, 1000
Sau96I GGNCC 4 cut(s) 4, 127, 220, 1079
SchI GAGTC 4 cut(s) 119, 178, 504, 890
ScrFI CCNGG 1 cut(s) 936
SduI GDGCHC 1 cut(s) 828
SfoI GGCGCC 1 cut(s) 253
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SinI GGWCC 1 cut(s) 127
SlaI CTCGAG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 3 cut(s) 726, 743, 1090
SseBI AGGCCT 1 cut(s) 518
SsiI CCGC 8 cut(s) 100, 175, 240, 283, 296, 323, 652, 1008
SspDI GGCGCC 1 cut(s) 251
SspMI CTAG 2 cut(s) 1106, 1139
SstI GAGCTC 1 cut(s) 828
StuI AGGCCT 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 934
StyI CCWWGG 1 cut(s) 967
TaaI ACNGT 3 cut(s) 611, 877, 1113
TaiI ACGT 3 cut(s) 204, 846, 1031
TaqI TCGA 4 cut(s) 66, 360, 513, 1034
TasI AATT 3 cut(s) 726, 743, 1090
TauI GCSGC 3 cut(s) 177, 286, 1011
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 638, 708, 1133
Tru9I TTAA 3 cut(s) 638, 708, 1133
TscAI CASTG 3 cut(s) 427, 598, 616
TseFI GTSAC 3 cut(s) 298, 611, 979
TseI GCWGC 6 cut(s) 211, 383, 525, 602, 911, 1011
Tsp45I GTSAC 3 cut(s) 298, 611, 979
TspDTI ATGAA 3 cut(s) 46, 346, 510
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 3 cut(s) 427, 598, 616
VpaK11BI GGWCC 1 cut(s) 127
XhoI CTCGAG 1 cut(s) 512
XmiI GTMKAC 1 cut(s) 1034
XspI CTAG 2 cut(s) 1106, 1139
ZraI GACGTC 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.