FvH4_7g12581

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000446
Physical Location & Seq
Forward (+)
11395607 .. 11397232
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12581.t1

Sequence Viewer

Length: 972 bp
ATGGCTCCATTGCCAAACCTCCCAGAAGTCCTAATCATCAACCCACCACCAGCCTTCATCTGTTTCGAATCCCACTTCACCCACAAGTTCAACATCCTCCGAGCATGGGAGTCCCCTCTTCCTCTAGACCAGTTCCTCACCACCCACGCCCACTCAGTCCAAGCCCTCCTCTGCCGCGCCGAGACTCCGGTCAACGCCGACCTCCTCCGTCTGCTACCGGCCCTGAAGATCGTGGTGTCCGCCAGCACCGGCGCTGATCAAGTGGACAAAGCGGAGTGCCGGCGGCGTGGGATTGCCGTGACCAATGCTGGAAATGCGTACTCGGAGGATGTTGCTGATCTCGCTGTTGGGTTGTTTCTCGATGTGAATAGGAAGATTTCGGCAGCTGATTCGTACGTGAGACGTGGGCTTTGGTCTAGCAGAGGAGATTATTCTCTTGGTTCCAAGATAGGTGGAAAGCGAGTTGGGGTTGTTGGATTGGGTAGCATTGGCTATGAAGTTGCCAAAAGACTCGAGGCATTTGGTTGCAAGATTTCATACAACTCAAGGAGTAAAAGGCAGTCTTTGTCATATCCCTTCTATTCCAATGTTAGTGAACTTGCAGCAGACAATGATGCTCTCATTATCTGTTGTGGGTTAACAGACGAAACCCGGCACATGATCAACAAGGAAGTCTTATCTGCATTGGGAAAAGATGGAGTGGTAGTTAATATAGGGCGCGGTGCAATTATTGATGAGAAAGCATTGGTCAGGTGTTTGGTGAATGGAGAGATTAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCAAAAGAGATCGTGGCGATGGATAATGTTGTTCTGTCTCCACATAAGGCTGTTTATACAGTAGAGTCTGTAAAGGAAGGAGGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAAACCTTTGGTTACTCCAGTCATATATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

324

Amino Acids

35.02

Weight (kDa)

6.4

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 46 - 320 1.1e-22 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 117 - 289 1.1e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 177, 720
AciI CCGC 5 cut(s) 175, 240, 272, 283, 720
AcuI CTGAAG 1 cut(s) 245
AfaI GTAC 2 cut(s) 320, 395
AfiI CCNNNNNNNGG 1 cut(s) 106
AgsI TTSAA 1 cut(s) 91
AjiI CACGTC 1 cut(s) 404
AluBI AGCT 2 cut(s) 386, 926
AluI AGCT 2 cut(s) 386, 926
Alw26I GTCTC 3 cut(s) 176, 394, 858
Ama87I CYCGRG 1 cut(s) 512
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 2 cut(s) 383, 602
ArsI GACNNNNNNTTYG 2 cut(s) 393, 425
AspLEI GCGC 3 cut(s) 179, 254, 720
AspS9I GGNCC 1 cut(s) 220
AsuC2I CCSGG 1 cut(s) 652
AsuHPI GGTGA 4 cut(s) 70, 130, 772, 911
AsuII TTCGAA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 512
BbvI GCAGC 2 cut(s) 395, 614
BccI CCATC 2 cut(s) 689, 829
BceAI ACGGC 1 cut(s) 281
BcgI CGANNNNNNTGC 2 cut(s) 372, 406
BclI TGATCA 2 cut(s) 256, 660
BcnI CCSGG 1 cut(s) 652
BcoDI GTCTC 3 cut(s) 176, 394, 858
BfaI CTAG 2 cut(s) 125, 417
BfoI RGCGCY 1 cut(s) 255
BisI GCNGC 4 cut(s) 175, 284, 384, 603
BlsI GCNGC 4 cut(s) 176, 285, 385, 604
Bme1390I CCNGG 1 cut(s) 652
BmeT110I CYCGRG 1 cut(s) 512
BmgBI CACGTC 1 cut(s) 404
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 2 cut(s) 6, 442
BmrFI CCNGG 1 cut(s) 652
BmsI GCATC 1 cut(s) 604
BoxI GACNNNNGTC 1 cut(s) 188
BpmI CTGGAG 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 652
BsaAI YACGTR 1 cut(s) 397
BsaWI WCCGGW 1 cut(s) 187
BsaXI ACNNNNNCTCC 4 cut(s) 690, 720, 888, 918
Bsc4I CCNNNNNNNGG 1 cut(s) 106
Bse118I RCCGGY 3 cut(s) 217, 248, 279
Bse1I ACTGG 2 cut(s) 130, 956
Bse3DI GCAATG 1 cut(s) 8
BseGI GGATG 3 cut(s) 93, 334, 796
BseLI CCNNNNNNNGG 1 cut(s) 106
BseMI GCAATG 1 cut(s) 8
BseMII CTCAG 1 cut(s) 168
BseNI ACTGG 2 cut(s) 130, 956
BseRI GAGGAG 3 cut(s) 158, 194, 438
BseXI GCAGC 2 cut(s) 395, 614
Bsh1236I CGCG 2 cut(s) 177, 720
BshFI GGCC 1 cut(s) 221
BsiHKCI CYCGRG 1 cut(s) 512
BsiSI CCGG 5 cut(s) 188, 218, 249, 280, 652
BsiWI CGTACG 1 cut(s) 393
BslFI GGGAC 1 cut(s) 97
BslI CCNNNNNNNGG 1 cut(s) 106
BsmAI GTCTC 3 cut(s) 176, 394, 858
BsmBI CGTCTC 1 cut(s) 394
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 221
BsoBI CYCGRG 1 cut(s) 512
Bsp119I TTCGAA 1 cut(s) 66
Bsp143I GATC 6 cut(s) 228, 256, 337, 660, 825, 909
BspACI CCGC 5 cut(s) 175, 240, 272, 283, 720
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 167
BspFNI CGCG 2 cut(s) 177, 720
BspHI TCATGA 1 cut(s) 968
BspLI GGNNCC 2 cut(s) 6, 442
BspT104I TTCGAA 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 8
BsrFI RCCGGY 3 cut(s) 217, 248, 279
BsrI ACTGG 2 cut(s) 130, 956
BssAI RCCGGY 3 cut(s) 217, 248, 279
BssMI GATC 6 cut(s) 228, 256, 337, 660, 825, 909
Bst4CI ACNGT 1 cut(s) 877
Bst6I CTCTTC 1 cut(s) 123
BstBAI YACGTR 1 cut(s) 397
BstBI TTCGAA 1 cut(s) 66
BstC8I GCNNGC 2 cut(s) 244, 281
BstDEI CTNAG 1 cut(s) 154
BstF5I GGATG 3 cut(s) 93, 334, 796
BstFNI CGCG 2 cut(s) 177, 720
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 3 cut(s) 179, 254, 720
BstKTI GATC 6 cut(s) 231, 259, 340, 663, 828, 912
BstMAI GTCTC 3 cut(s) 176, 394, 858
BstMBI GATC 6 cut(s) 228, 256, 337, 660, 825, 909
BstMWI GCNNNNNNNGC 2 cut(s) 314, 341
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 1 cut(s) 650
BstUI CGCG 2 cut(s) 177, 720
BstV1I GCAGC 2 cut(s) 395, 614
BsuRI GGCC 1 cut(s) 221
BtgZI GCGATG 1 cut(s) 848
BtrI CACGTC 1 cut(s) 404
BtsCI GGATG 3 cut(s) 93, 334, 796
Cac8I GCNNGC 2 cut(s) 244, 281
CciI TCATGA 1 cut(s) 968
CfoI GCGC 3 cut(s) 179, 254, 720
Cfr10I RCCGGY 3 cut(s) 217, 248, 279
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 2 cut(s) 319, 394
CspCI CAANNNNNGTGG 2 cut(s) 433, 468
CviAII CATG 3 cut(s) 105, 658, 969
CviQI GTAC 2 cut(s) 319, 394
DdeI CTNAG 1 cut(s) 154
DpnI GATC 6 cut(s) 230, 258, 339, 662, 827, 911
DpnII GATC 6 cut(s) 228, 256, 337, 660, 825, 909
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 512
Esp3I CGTCTC 1 cut(s) 394
FaeI CATG 3 cut(s) 108, 661, 972
FalI AAGNNNNNCTT 4 cut(s) 547, 579, 659, 691
FaqI GGGAC 1 cut(s) 97
FatI CATG 3 cut(s) 104, 657, 968
FbaI TGATCA 2 cut(s) 256, 660
Fnu4HI GCNGC 4 cut(s) 175, 284, 384, 603
FokI GGATG 3 cut(s) 80, 341, 803
Fsp4HI GCNGC 4 cut(s) 175, 284, 384, 603
FspBI CTAG 2 cut(s) 125, 417
GlaI GCGC 3 cut(s) 178, 253, 719
GluI GCNGC 4 cut(s) 175, 284, 384, 603
GsuI CTGGAG 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 1 cut(s) 221
HapII CCGG 5 cut(s) 188, 218, 249, 280, 652
HhaI GCGC 3 cut(s) 179, 254, 720
Hin1II CATG 3 cut(s) 108, 661, 972
Hin6I GCGC 3 cut(s) 177, 252, 718
HinP1I GCGC 3 cut(s) 177, 252, 718
HincII GTYRAC 2 cut(s) 193, 639
HindII GTYRAC 2 cut(s) 193, 639
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 6 cut(s) 68, 110, 184, 389, 510, 881
HpaI GTTAAC 1 cut(s) 639
HpaII CCGG 5 cut(s) 188, 218, 249, 280, 652
HphI GGTGA 4 cut(s) 70, 130, 772, 911
Hpy166II GTNNAC 4 cut(s) 193, 265, 596, 639
Hpy188I TCNGA 2 cut(s) 101, 325
Hpy188III TCNNGA 3 cut(s) 125, 359, 969
Hpy8I GTNNAC 4 cut(s) 193, 265, 596, 639
HpyAV CCTTC 3 cut(s) 64, 586, 887
HpyCH4III ACNGT 1 cut(s) 877
HpyCH4IV ACGT 2 cut(s) 396, 403
HpyCH4V TGCA 4 cut(s) 528, 602, 683, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 341
HpyF3I CTNAG 1 cut(s) 154
HpySE526I ACGT 2 cut(s) 396, 403
Hsp92II CATG 3 cut(s) 108, 661, 972
HspAI GCGC 3 cut(s) 177, 252, 718
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 2 cut(s) 256, 660
KspAI GTTAAC 1 cut(s) 639
Kzo9I GATC 6 cut(s) 228, 256, 337, 660, 825, 909
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 2 cut(s) 395, 614
LweI GCATC 1 cut(s) 604
MaeI CTAG 2 cut(s) 125, 417
MaeII ACGT 2 cut(s) 396, 403
MaeIII GTNAC 2 cut(s) 298, 949
MalI GATC 6 cut(s) 230, 258, 339, 662, 827, 911
MboI GATC 6 cut(s) 228, 256, 337, 660, 825, 909
MboII GAAGA 4 cut(s) 110, 238, 385, 922
MluCI AATT 2 cut(s) 726, 916
MlyI GAGTC 4 cut(s) 119, 178, 504, 890
MmeI TCCRAC 1 cut(s) 454
MroNI GCCGGC 1 cut(s) 279
MseI TTAA 2 cut(s) 638, 708
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 5 cut(s) 188, 218, 249, 280, 652
MspR9I CCNGG 1 cut(s) 652
MvnI CGCG 2 cut(s) 177, 720
MwoI GCNNNNNNNGC 2 cut(s) 314, 341
NaeI GCCGGC 1 cut(s) 281
NciI CCSGG 1 cut(s) 652
NdeII GATC 6 cut(s) 228, 256, 337, 660, 825, 909
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 3 cut(s) 108, 661, 972
NlaIV GGNNCC 2 cut(s) 6, 442
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 1 cut(s) 298
NspV TTCGAA 1 cut(s) 66
PaeR7I CTCGAG 1 cut(s) 512
PagI TCATGA 1 cut(s) 968
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 2 cut(s) 68, 389
Pfl23II CGTACG 1 cut(s) 393
PkrI GCNGC 4 cut(s) 176, 285, 385, 604
PleI GAGTC 4 cut(s) 118, 178, 504, 889
PpsI GAGTC 4 cut(s) 118, 178, 504, 889
Ppu21I YACGTR 1 cut(s) 397
PshAI GACNNNNGTC 1 cut(s) 188
PspLI CGTACG 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 6, 442
PspPI GGNCC 1 cut(s) 220
PspXI VCTCGAGB 1 cut(s) 512
PvuII CAGCTG 1 cut(s) 386
RsaI GTAC 2 cut(s) 320, 395
RsaNI GTAC 2 cut(s) 319, 394
SaqAI TTAA 2 cut(s) 638, 708
SatI GCNGC 4 cut(s) 175, 284, 384, 603
Sau3AI GATC 6 cut(s) 228, 256, 337, 660, 825, 909
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 4 cut(s) 119, 178, 504, 890
ScrFI CCNGG 1 cut(s) 652
SfaNI GCATC 1 cut(s) 604
Sfr274I CTCGAG 1 cut(s) 512
SfuI TTCGAA 1 cut(s) 66
SgrAI CRCCGGYG 1 cut(s) 248
SlaI CTCGAG 1 cut(s) 512
SmlI CTYRAG 2 cut(s) 512, 544
SmoI CTYRAG 2 cut(s) 512, 544
Sse9I AATT 2 cut(s) 726, 916
SsiI CCGC 5 cut(s) 175, 240, 272, 283, 720
SspMI CTAG 2 cut(s) 125, 417
StyD4I CCNGG 1 cut(s) 650
TaaI ACNGT 1 cut(s) 877
TaiI ACGT 2 cut(s) 399, 406
TaqI TCGA 3 cut(s) 66, 360, 513
TasI AATT 2 cut(s) 726, 916
TauI GCSGC 2 cut(s) 177, 286
TfiI GAWTC 2 cut(s) 68, 389
Tru1I TTAA 2 cut(s) 638, 708
Tru9I TTAA 2 cut(s) 638, 708
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 2 cut(s) 383, 602
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 4 cut(s) 46, 510, 525, 957
TspGWI ACGGA 1 cut(s) 197
XbaI TCTAGA 1 cut(s) 124
XcmI CCANNNNNNNNNTGG 1 cut(s) 943
XhoI CTCGAG 1 cut(s) 512
XspI CTAG 2 cut(s) 125, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.