Rh1AG233300

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000446
Physical Location & Seq
Forward (+)
44366623 .. 44367890
1268 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG233300.1

Sequence Viewer

Length: 981 bp
ATGGCGGATGATAATCAAAGTGAGCTCCCTACAGTTTTGATACTTCGCCAATCCGGATTCATTGGTCTCATGGAACCCAAACTCTCCCAAAAATTCAACTTACTGAAAGCCTGGGACTCTCCTCTCCCTCAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCCCTTATCTCCTCCGCCGATGGGCCACCCATCACCGCCCAAATCCTCCACTTGCTCCCCTCCCTCAAAGTCATAGCCACCACCAGCGCCGGCATTGACCACGTTGACTTGGCCGAGTGTCGACGCCGCGGCGTTGCCGTCGCCGACGCCGGTAAAGCCTTTGCTGAGGACGCTGCTGACTCGGCTGTGGGTCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCAGTATGTTAGGGATGGGCTCTGGGCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTCGAAGTTGCAAAAAGATTGGAGGCATTTGGCTGCAGTGTGTCGTACAATTCGAGGAGCGAAAGGCCGTATCTGTCATACCAGTTCTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATCATCTGTTGCGCATTGACAGAGCAAACCCACCACATGATCAACAAGGAAGTTTTGTCTGCGTTGGGAAGAGAGGGGGTGGTTGTTAATGTAGGGCGTGGAGCCATTATCGATGAGAAAGAAATGGTGCAGTGTTTGGTGCGAGGAGAGATAAGGGGTGCTGGCCTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTTTGCATTGGATAATGTTGTACTGTCACCGCATCAAGCTGTTCTTACACATGAAGCTTTCGTGGGTTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCCGGCTGTGATTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

326

Amino Acids

35.1

Weight (kDa)

5.16

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 39 - 321 2.1e-18 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 2.7e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 643
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 289
AccII CGCG 1 cut(s) 297
AccIII TCCGGA 1 cut(s) 53
AciI CCGC 7 cut(s) 5, 157, 183, 204, 295, 297, 866
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 92, 925
AcyI GRCGYC 2 cut(s) 292, 315
AfaI GTAC 2 cut(s) 548, 858
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 3 cut(s) 110, 165, 795
AluBI AGCT 5 cut(s) 25, 607, 835, 875, 893
AluI AGCT 5 cut(s) 25, 607, 835, 875, 893
Alw21I GWGCWC 2 cut(s) 27, 837
Alw26I GTCTC 2 cut(s) 71, 429
Aor13HI TCCGGA 1 cut(s) 53
AoxI GGCC 5 cut(s) 168, 191, 279, 566, 793
ApeKI GCWGC 3 cut(s) 341, 534, 611
ApoI RAATTY 2 cut(s) 92, 925
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 2 cut(s) 257, 644
AspS9I GGNCC 2 cut(s) 169, 191
AsuHPI GGTGA 3 cut(s) 193, 855, 920
AsuII TTCGAA 2 cut(s) 395, 504
BanII GRGCYC 3 cut(s) 27, 420, 837
Bbv12I GWGCWC 2 cut(s) 27, 837
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 328, 521, 623
BccI CCATC 3 cut(s) 182, 206, 407
BceAI ACGGC 2 cut(s) 290, 553
BciT130I CCWGG 3 cut(s) 112, 167, 797
BclI TGATCA 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 71, 429
BfmI CTRYAG 2 cut(s) 30, 535
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 5 cut(s) 295, 298, 342, 535, 612
BlsI GCNGC 5 cut(s) 296, 299, 343, 536, 613
Bme1390I CCNGG 3 cut(s) 112, 167, 797
BmgT120I GGNCC 2 cut(s) 169, 191
BmiI GGNNCC 2 cut(s) 75, 733
BmrFI CCNGG 3 cut(s) 112, 167, 797
BmsI GCATC 2 cut(s) 613, 877
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 2 cut(s) 395, 504
BpuEI CTTGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 2 cut(s) 292, 315
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 2 cut(s) 111, 295
BsaWI WCCGGW 1 cut(s) 53
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 2 cut(s) 257, 317
Bse1I ACTGG 2 cut(s) 136, 583
Bse8I GATNNNNATC 1 cut(s) 12
BseAI TCCGGA 1 cut(s) 53
BseBI CCWGG 3 cut(s) 112, 167, 797
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 3 cut(s) 13, 418, 805
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 2 cut(s) 136, 583
BseRI GAGGAG 5 cut(s) 111, 169, 447, 571, 789
BseXI GCAGC 3 cut(s) 328, 521, 623
BsgI GTGCAG 1 cut(s) 779
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 5 cut(s) 170, 193, 281, 568, 795
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 2 cut(s) 27, 837
BsiSI CCGG 4 cut(s) 54, 258, 318, 965
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 71, 429
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 5 cut(s) 170, 193, 281, 568, 795
Bso31I GGTCTC 1 cut(s) 71
Bsp119I TTCGAA 2 cut(s) 395, 504
Bsp1286I GDGCHC 3 cut(s) 27, 420, 837
Bsp13I TCCGGA 1 cut(s) 53
Bsp143I GATC 1 cut(s) 669
BspACI CCGC 7 cut(s) 5, 157, 183, 204, 295, 297, 866
BspANI GGCC 5 cut(s) 170, 193, 281, 568, 795
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 741
BspEI TCCGGA 1 cut(s) 53
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 2 cut(s) 75, 733
BspMAI CTGCAG 1 cut(s) 539
BspT104I TTCGAA 2 cut(s) 395, 504
BspTNI GGTCTC 1 cut(s) 71
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 257, 317
BsrI ACTGG 2 cut(s) 136, 583
BssAI RCCGGY 2 cut(s) 257, 317
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 1 cut(s) 669
BssNI GRCGYC 2 cut(s) 292, 315
Bst2UI CCWGG 3 cut(s) 112, 167, 797
Bst4CI ACNGT 4 cut(s) 34, 620, 861, 954
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 2 cut(s) 292, 315
BstBI TTCGAA 2 cut(s) 395, 504
BstC8I GCNNGC 4 cut(s) 157, 259, 609, 793
BstDEI CTNAG 2 cut(s) 333, 453
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 3 cut(s) 13, 418, 805
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 257, 644
BstKTI GATC 1 cut(s) 672
BstMAI GTCTC 2 cut(s) 71, 429
BstMBI GATC 1 cut(s) 669
BstMWI GCNNNNNNNGC 3 cut(s) 323, 338, 350
BstNI CCWGG 3 cut(s) 112, 167, 797
BstSCI CCNGG 3 cut(s) 110, 165, 795
BstSFI CTRYAG 2 cut(s) 30, 535
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 328, 521, 623
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 5 cut(s) 170, 193, 281, 568, 795
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 3 cut(s) 13, 418, 805
BtsI GCAGTG 2 cut(s) 544, 767
BtsIMutI CAGTG 3 cut(s) 544, 625, 767
Cac8I GCNNGC 4 cut(s) 157, 259, 609, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 257, 644
Cfr10I RCCGGY 2 cut(s) 257, 317
Cfr13I GGNCC 2 cut(s) 169, 191
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 3 cut(s) 300, 323, 347
Csp6I GTAC 2 cut(s) 547, 857
CviAII CATG 4 cut(s) 70, 376, 667, 887
CviQI GTAC 2 cut(s) 547, 857
DdeI CTNAG 2 cut(s) 333, 453
DpnI GATC 1 cut(s) 671
DpnII GATC 1 cut(s) 669
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
EciI GGCGGA 2 cut(s) 20, 172
Ecl136II GAGCTC 2 cut(s) 25, 835
Eco24I GRGCYC 3 cut(s) 27, 420, 837
Eco31I GGTCTC 1 cut(s) 71
Eco53kI GAGCTC 2 cut(s) 25, 835
EcoICRI GAGCTC 2 cut(s) 25, 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 110, 165, 795
EcoT38I GRGCYC 3 cut(s) 27, 420, 837
FaeI CATG 4 cut(s) 73, 379, 670, 890
FaiI YATR 7 cut(s) 71, 242, 377, 405, 580, 668, 888
FalI AAGNNNNNCTT 2 cut(s) 864, 896
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 69, 375, 666, 886
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 5 cut(s) 295, 298, 342, 535, 612
FokI GGATG 3 cut(s) 20, 425, 812
FriOI GRGCYC 3 cut(s) 27, 420, 837
Fsp4HI GCNGC 5 cut(s) 295, 298, 342, 535, 612
FspI TGCGCA 1 cut(s) 643
GlaI GCGC 2 cut(s) 256, 643
GluI GCNGC 5 cut(s) 295, 298, 342, 535, 612
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 5 cut(s) 170, 193, 281, 568, 795
HapII CCGG 4 cut(s) 54, 258, 318, 965
HgaI GACGC 3 cut(s) 300, 323, 347
HhaI GCGC 2 cut(s) 257, 644
Hin1I GRCGYC 2 cut(s) 292, 315
Hin1II CATG 4 cut(s) 73, 379, 670, 890
Hin6I GCGC 2 cut(s) 255, 642
HinP1I GCGC 2 cut(s) 255, 642
HincII GTYRAC 2 cut(s) 274, 290
HindII GTYRAC 2 cut(s) 274, 290
HindIII AAGCTT 1 cut(s) 891
HinfI GANTC 4 cut(s) 57, 116, 347, 397
HpaII CCGG 4 cut(s) 54, 258, 318, 965
HphI GGTGA 3 cut(s) 193, 855, 920
Hpy166II GTNNAC 2 cut(s) 274, 290
Hpy188III TCNNGA 4 cut(s) 54, 131, 142, 376
Hpy8I GTNNAC 2 cut(s) 274, 290
Hpy99I CGWCG 3 cut(s) 294, 311, 317
HpyAV CCTTC 1 cut(s) 463
HpyCH4III ACNGT 4 cut(s) 34, 620, 861, 954
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 5 cut(s) 512, 537, 611, 760, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 323, 338, 350
HpyF3I CTNAG 2 cut(s) 333, 453
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 2 cut(s) 292, 315
Hsp92II CATG 4 cut(s) 73, 379, 670, 890
HspAI GCGC 2 cut(s) 255, 642
Kpn2I TCCGGA 1 cut(s) 53
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 669
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 1 cut(s) 669
LmnI GCTCC 5 cut(s) 30, 164, 228, 558, 731
Lsp1109I GCAGC 3 cut(s) 328, 521, 623
LweI GCATC 2 cut(s) 613, 877
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 861, 916
MalI GATC 1 cut(s) 671
MbiI CCGCTC 1 cut(s) 159
MboI GATC 1 cut(s) 669
MboII GAAGA 2 cut(s) 711, 931
MhlI GDGCHC 3 cut(s) 27, 420, 837
MluCI AATT 4 cut(s) 92, 550, 911, 925
MlyI GAGTC 2 cut(s) 110, 341
MmeI TCCRAC 1 cut(s) 463
MroI TCCGGA 1 cut(s) 53
MroNI GCCGGC 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 1 cut(s) 717
MspA1I CMGCKG 1 cut(s) 297
MspI CCGG 4 cut(s) 54, 258, 318, 965
MspR9I CCNGG 3 cut(s) 112, 167, 797
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 3 cut(s) 112, 167, 797
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 3 cut(s) 323, 338, 350
NaeI GCCGGC 1 cut(s) 259
NdeII GATC 1 cut(s) 669
NgoMIV GCCGGC 1 cut(s) 257
NlaIII CATG 4 cut(s) 73, 379, 670, 890
NlaIV GGNNCC 2 cut(s) 75, 733
NmeAIII GCCGAG 2 cut(s) 307, 329
NmuCI GTSAC 1 cut(s) 861
NsbI TGCGCA 1 cut(s) 643
NspV TTCGAA 2 cut(s) 395, 504
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 551
PctI GAATGC 1 cut(s) 935
PdiI GCCGGC 1 cut(s) 259
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 2 cut(s) 57, 397
PkrI GCNGC 5 cut(s) 296, 299, 343, 536, 613
PleI GAGTC 2 cut(s) 110, 341
PpsI GAGTC 2 cut(s) 110, 341
Psp124BI GAGCTC 2 cut(s) 27, 837
Psp6I CCWGG 3 cut(s) 110, 165, 795
PspGI CCWGG 3 cut(s) 110, 165, 795
PspN4I GGNNCC 2 cut(s) 75, 733
PspPI GGNCC 2 cut(s) 169, 191
PstI CTGCAG 1 cut(s) 539
RsaI GTAC 2 cut(s) 548, 858
RsaNI GTAC 2 cut(s) 547, 857
SacI GAGCTC 2 cut(s) 27, 837
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 5 cut(s) 295, 298, 342, 535, 612
Sau3AI GATC 1 cut(s) 669
Sau96I GGNCC 2 cut(s) 169, 191
SchI GAGTC 2 cut(s) 110, 341
ScrFI CCNGG 3 cut(s) 112, 167, 797
SduI GDGCHC 3 cut(s) 27, 420, 837
SetI ASST 7 cut(s) 27, 273, 474, 609, 837, 877, 895
SfaNI GCATC 2 cut(s) 613, 877
SfcI CTRYAG 2 cut(s) 30, 535
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 2 cut(s) 395, 504
SgrBI CCGCGG 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 4 cut(s) 92, 550, 911, 925
SsiI CCGC 7 cut(s) 5, 157, 183, 204, 295, 297, 866
SstI GAGCTC 2 cut(s) 27, 837
StyD4I CCNGG 3 cut(s) 110, 165, 795
TaaI ACNGT 4 cut(s) 34, 620, 861, 954
TaiI ACGT 1 cut(s) 273
TaqI TCGA 5 cut(s) 289, 395, 504, 554, 741
TasI AATT 4 cut(s) 92, 550, 911, 925
TatI WGTACW 1 cut(s) 856
TauI GCSGC 2 cut(s) 297, 300
TfiI GAWTC 2 cut(s) 57, 397
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 3 cut(s) 544, 625, 767
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 3 cut(s) 341, 534, 611
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 3 cut(s) 49, 355, 903
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 3 cut(s) 544, 625, 767
XapI RAATTY 2 cut(s) 92, 925
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.