Rw1G020300

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000446
Physical Location & Seq
Forward (+)
43957109 .. 43958226
1118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020300.1

Sequence Viewer

Length: 981 bp
ATGGCAGATGGTAATCACAGTGAGCTCCCTACAGTTCTGATACTTCGCCGATGCCCAACCATTGCTCTCTTGGAACCCATAGTCTCTCAAAAATTCAACTTACTGAAAGCCTGGGACTCTCTTCTCCCTCAAGACCAGTTCTTGACCACCCACGCCAGCTCCGTCCAGGCCCTTATCTCCTCCGCCGATGGGCCACCCATAACCGCCCAAATCCTCCACTTGCTCCCCTCCCTCAAAGTCATAGCCACCACCAGCGCCGTCCTTGACCACGTTGACTTGGCCGAGTGTCGACGCCGCGGCGTTGCCGTCGTCGACGCCGGTGAAGCCTTTGCTGAGGACGCTGCTGACTCGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCGTTATGTTAGGGATGGGCTCTGGGCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTAGAAGTTGCAAAACGATTGGAGGCATTTGGCTGCACTGTCTTGTACAATTCGAGGAGTGAAAAGCCATCTTTGTCATACCAATTTTATTCCAATGTCTGTGAGCTTGCAGCAGACAGTGATGCTCTCATAATCTGCTGTGCATTGACAGCGGAAACCCACCACATGATCAACAAGGAAGTATTGTCTGCATTGGGAAGAGAGGGCGTCATTGTTAATGTAGGGCGCAGAGTTATTATCGATGAGAAGGAAATGGTGCAGTGTTTGGTGAGAGGAGAGATAAGGGGTGCTGGCTTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGATCTCTTTGCATTGGATAATGTTGTACTGTCACTGCATCAAGCTGTCGCTACCCATGAAGCTATCATGGCTTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCCGGCTGTGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

326

Amino Acids

35.06

Weight (kDa)

5.31

Isoelectric Point (pI)

34.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 48 - 321 6.1e-18 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 3.5e-47 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 289, 312
AccII CGCG 1 cut(s) 297
AciI CCGC 5 cut(s) 183, 204, 295, 297, 653
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 92, 925
AcyI GRCGYC 3 cut(s) 292, 315, 708
AfaI GTAC 2 cut(s) 548, 858
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 110, 165
AluBI AGCT 5 cut(s) 25, 159, 607, 875, 893
AluI AGCT 5 cut(s) 25, 159, 607, 875, 893
Alw21I GWGCWC 1 cut(s) 27
Alw26I GTCTC 2 cut(s) 88, 429
AoxI GGCC 3 cut(s) 168, 191, 279
ApeKI GCWGC 3 cut(s) 341, 534, 611
ApoI RAATTY 2 cut(s) 92, 925
AseI ATTAAT 1 cut(s) 975
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 2 cut(s) 257, 729
AspS9I GGNCC 2 cut(s) 169, 191
AsuHPI GGTGA 3 cut(s) 332, 781, 920
AsuII TTCGAA 1 cut(s) 395
BanII GRGCYC 2 cut(s) 27, 420
Bbv12I GWGCWC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 328, 521, 623
BccI CCATC 4 cut(s) 2, 182, 407, 577
BceAI ACGGC 2 cut(s) 242, 290
BciT130I CCWGG 2 cut(s) 112, 167
BclI TGATCA 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 88, 429
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 258
BglII AGATCT 1 cut(s) 832
BisI GCNGC 5 cut(s) 295, 298, 342, 535, 612
BlsI GCNGC 5 cut(s) 296, 299, 343, 536, 613
Bme1390I CCNGG 2 cut(s) 112, 167
BmgT120I GGNCC 2 cut(s) 169, 191
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 2 cut(s) 112, 167
BmsI GCATC 3 cut(s) 41, 613, 877
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 3 cut(s) 292, 315, 708
BsaJI CCNNGG 2 cut(s) 111, 295
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 1 cut(s) 317
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 112, 167
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 2 cut(s) 418, 805
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 4 cut(s) 169, 447, 571, 789
BseXI GCAGC 3 cut(s) 328, 521, 623
BsgI GTGCAG 2 cut(s) 520, 779
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 170, 193, 281
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 1 cut(s) 27
BsiSI CCGG 2 cut(s) 318, 965
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 189
BsmAI GTCTC 2 cut(s) 88, 429
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 3 cut(s) 170, 193, 281
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 27, 420
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 669, 832
BspACI CCGC 5 cut(s) 183, 204, 295, 297, 653
BspANI GGCC 3 cut(s) 170, 193, 281
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 75
BspT104I TTCGAA 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 317
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 2 cut(s) 669, 832
BssNI GRCGYC 3 cut(s) 292, 315, 708
Bst2UI CCWGG 2 cut(s) 112, 167
Bst4CI ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
Bst6I CTCTTC 2 cut(s) 126, 694
BstACI GRCGYC 3 cut(s) 292, 315, 708
BstAUI TGTACA 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 3 cut(s) 157, 609, 793
BstDEI CTNAG 2 cut(s) 333, 453
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 418, 805
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 257, 729
BstKTI GATC 2 cut(s) 672, 835
BstMAI GTCTC 2 cut(s) 88, 429
BstMBI GATC 2 cut(s) 669, 832
BstMWI GCNNNNNNNGC 5 cut(s) 323, 338, 350, 650, 899
BstNI CCWGG 2 cut(s) 112, 167
BstSCI CCNGG 2 cut(s) 110, 165
BstSFI CTRYAG 1 cut(s) 30
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 328, 521, 623
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 3 cut(s) 170, 193, 281
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 418, 805
BtsI GCAGTG 2 cut(s) 767, 863
BtsIMutI CAGTG 5 cut(s) 25, 537, 625, 767, 863
Cac8I GCNNGC 3 cut(s) 157, 609, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 257, 729
Cfr10I RCCGGY 1 cut(s) 317
Cfr13I GGNCC 2 cut(s) 169, 191
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 4 cut(s) 300, 323, 347, 697
Csp6I GTAC 2 cut(s) 547, 857
CviAII CATG 4 cut(s) 376, 667, 887, 898
CviQI GTAC 2 cut(s) 547, 857
DdeI CTNAG 2 cut(s) 333, 453
DpnI GATC 2 cut(s) 671, 834
DpnII GATC 2 cut(s) 669, 832
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 126, 694
EarI CTCTTC 2 cut(s) 126, 694
EciI GGCGGA 1 cut(s) 172
Ecl136II GAGCTC 1 cut(s) 25
Eco24I GRGCYC 2 cut(s) 27, 420
Eco53kI GAGCTC 1 cut(s) 25
EcoICRI GAGCTC 1 cut(s) 25
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 110, 165
EcoT38I GRGCYC 2 cut(s) 27, 420
FaeI CATG 4 cut(s) 379, 670, 890, 901
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 375, 666, 886, 897
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 2 cut(s) 289, 312
Fnu4HI GCNGC 5 cut(s) 295, 298, 342, 535, 612
FokI GGATG 2 cut(s) 425, 812
FriOI GRGCYC 2 cut(s) 27, 420
Fsp4HI GCNGC 5 cut(s) 295, 298, 342, 535, 612
GlaI GCGC 2 cut(s) 256, 728
GluI GCNGC 5 cut(s) 295, 298, 342, 535, 612
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 3 cut(s) 170, 193, 281
HapII CCGG 2 cut(s) 318, 965
HgaI GACGC 4 cut(s) 300, 323, 347, 697
HhaI GCGC 2 cut(s) 257, 729
Hin1I GRCGYC 3 cut(s) 292, 315, 708
Hin1II CATG 4 cut(s) 379, 670, 890, 901
Hin6I GCGC 2 cut(s) 255, 727
HinP1I GCGC 2 cut(s) 255, 727
HincII GTYRAC 3 cut(s) 274, 290, 313
HindII GTYRAC 3 cut(s) 274, 290, 313
HinfI GANTC 3 cut(s) 116, 347, 397
HpaII CCGG 2 cut(s) 318, 965
HphI GGTGA 3 cut(s) 332, 781, 920
Hpy166II GTNNAC 3 cut(s) 274, 290, 313
Hpy188I TCNGA 1 cut(s) 39
Hpy188III TCNNGA 3 cut(s) 131, 142, 376
Hpy8I GTNNAC 3 cut(s) 274, 290, 313
Hpy99I CGWCG 4 cut(s) 294, 311, 314, 317
HpyAV CCTTC 2 cut(s) 463, 742
HpyCH4III ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 8 cut(s) 512, 537, 611, 644, 692, 760, 842, 868
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 338, 350, 650, 899
HpyF3I CTNAG 2 cut(s) 333, 453
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 3 cut(s) 292, 315, 708
Hsp92II CATG 4 cut(s) 379, 670, 890, 901
HspAI GCGC 2 cut(s) 255, 727
Ksp22I TGATCA 1 cut(s) 669
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 2 cut(s) 669, 832
LmnI GCTCC 3 cut(s) 30, 164, 228
Lsp1109I GCAGC 3 cut(s) 328, 521, 623
LweI GCATC 3 cut(s) 41, 613, 877
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 861, 916
MalI GATC 2 cut(s) 671, 834
MboI GATC 2 cut(s) 669, 832
MboII GAAGA 3 cut(s) 113, 711, 931
MflI RGATCY 1 cut(s) 832
MhlI GDGCHC 2 cut(s) 27, 420
MluCI AATT 6 cut(s) 92, 550, 584, 911, 925, 976
MlyI GAGTC 2 cut(s) 110, 341
MmeI TCCRAC 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 2 cut(s) 717, 975
MspA1I CMGCKG 2 cut(s) 297, 653
MspI CCGG 2 cut(s) 318, 965
MspR9I CCNGG 2 cut(s) 112, 167
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 2 cut(s) 112, 167
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 5 cut(s) 323, 338, 350, 650, 899
NdeII GATC 2 cut(s) 669, 832
NlaIII CATG 4 cut(s) 379, 670, 890, 901
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 2 cut(s) 307, 329
NmuCI GTSAC 1 cut(s) 861
NspV TTCGAA 1 cut(s) 395
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 5 cut(s) 296, 299, 343, 536, 613
PleI GAGTC 2 cut(s) 110, 341
PpsI GAGTC 2 cut(s) 110, 341
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 27
Psp6I CCWGG 2 cut(s) 110, 165
PspGI CCWGG 2 cut(s) 110, 165
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 2 cut(s) 169, 191
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 548, 858
RsaNI GTAC 2 cut(s) 547, 857
SacI GAGCTC 1 cut(s) 27
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 2 cut(s) 288, 311
SaqAI TTAA 2 cut(s) 717, 975
SatI GCNGC 5 cut(s) 295, 298, 342, 535, 612
Sau3AI GATC 2 cut(s) 669, 832
Sau96I GGNCC 2 cut(s) 169, 191
SchI GAGTC 2 cut(s) 110, 341
ScrFI CCNGG 2 cut(s) 112, 167
SduI GDGCHC 2 cut(s) 27, 420
SetI ASST 7 cut(s) 27, 161, 273, 474, 609, 877, 895
SfaNI GCATC 3 cut(s) 41, 613, 877
SfcI CTRYAG 1 cut(s) 30
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 395
SgrAI CRCCGGYG 1 cut(s) 317
SgrBI CCGCGG 1 cut(s) 298
SgrDI CGTCGACG 1 cut(s) 311
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 6 cut(s) 92, 550, 584, 911, 925, 976
SsiI CCGC 5 cut(s) 183, 204, 295, 297, 653
SstI GAGCTC 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 110, 165
TaaI ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
TaiI ACGT 1 cut(s) 273
TaqI TCGA 5 cut(s) 289, 312, 395, 554, 741
TasI AATT 6 cut(s) 92, 550, 584, 911, 925, 976
TatI WGTACW 2 cut(s) 546, 856
TauI GCSGC 2 cut(s) 297, 300
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 2 cut(s) 717, 975
Tru9I TTAA 2 cut(s) 717, 975
TscAI CASTG 5 cut(s) 25, 544, 625, 767, 870
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 3 cut(s) 341, 534, 611
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 2 cut(s) 355, 903
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 5 cut(s) 25, 544, 625, 767, 870
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 2 cut(s) 92, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmiI GTMKAC 2 cut(s) 289, 312
XmnI GAANNNNTTC 1 cut(s) 385
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.