Rh1CG218100

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0000446
Physical Location & Seq
Forward (+)
46867217 .. 46869257
2041 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG218100.1

Sequence Viewer

Length: 1101 bp
ATGCGAGACATTTTATCGCAAGGAGCATTAAACATACAAGAACTTCAGCTTGTAAAATCAGTGTCGGTACAGTTTATCATTTGGGAACAAAAAGCAAAACTAATCTTGATCTCACTCTCAAAGTCTCTAATGGCCCAGCAGTTGCAAAACCTCCCAGATGTCCTGATCATCAAACCACCACCAGCATTCATATCTTTCGAATCCGACTTCACCCAGAAGTTCAACATCCTCCGAGCATGGGAATCGCCACTCCCTCTGGACCAGTTCCTCACCACCCACGCCCAATCAGTCCAGGCCCTCCTTTGCCGCGCTGTGACTCAGGTCAACGCCGACCTCCTCCAGCTGCTACCGGCCCTGAAGCTCGTGGTGACTGTCAGCACCGGCGCCAATCACGTGGACATAGCGGAGTGCCGGCGGCGTGGGATTGCTGTGGCCAATGCCCGAAATGCGTACTCGGAAGATGTTGCTGATATCGCTGTTGGGTTGTTCATCGACGTGAATCGGAGAATTTCAGCAGCTGATTGGTATGTGAGACGTGGGCTTTGGTCTAGCAAAGGAGACTATCCTCTTGGTTCCAAGATAGGTGGAAAGCGTGTAGGTATTGTTGGATTGGGAAGCATTGGCTATGAAGTTGCCAAAAGACTTGAGACCTTTGGCTGCAAGATCTCATACAACTCTAGGAGTGAAAGGCCATCTTTGTCGTACCCCTTCTATTCCAATGTCTGTGAGCTTGCAGCTAATAATGATGCTCTCATCATCTGTTGTGCATTGACAGACCAAACCCGCCACATGATCAACAAGGAAGTCTTATTTGCATTGGGAAAAGATGGAGTGATAGTTAATATAGGGCGTGGGGCAATTATTGATGAAAAAGAATTGGTCAAGTGTTTGGTGAATGGAGAGATCAGAGGTGCTGGTTTGGATGTGTTTGAGAATGAGCCTCATATACCGAAAGACCTTGTGGCAATGGACAATGTTGTTCTGTCTCCACATAAGTCTGTTTATACCCTAGAGTCTTTCAAGAGAGGAAGTGAAGTAATGATCGCCAATTTGGAAGCTTTCTTCTCCAATAGACCATTGATTACTCCAGTCTATGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

366

Amino Acids

40.37

Weight (kDa)

8.09

Isoelectric Point (pI)

42.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 90 - 364 1.7e-22 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 161 - 331 3e-48 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 1094
AccII CGCG 1 cut(s) 309
AciI CCGC 4 cut(s) 307, 404, 415, 784
AcoI YGGCCR 1 cut(s) 432
AcsI RAATTY 1 cut(s) 507
AcuI CTGAAG 2 cut(s) 29, 377
AcvI CACGTG 1 cut(s) 394
AcyI GRCGYC 1 cut(s) 384
AfaI GTAC 3 cut(s) 69, 452, 704
AfiI CCNNNNNNNGG 2 cut(s) 238, 1094
AgsI TTSAA 2 cut(s) 223, 1021
AjiI CACGTC 2 cut(s) 496, 536
AjnI CCWGG 1 cut(s) 291
AluBI AGCT 7 cut(s) 49, 343, 361, 518, 730, 737, 1058
AluI AGCT 7 cut(s) 49, 343, 361, 518, 730, 737, 1058
Alw26I GTCTC 5 cut(s) 129, 526, 552, 641, 990
AlwNI CAGNNNCTG 1 cut(s) 518
AoxI GGCC 5 cut(s) 132, 294, 351, 432, 689
ApeKI GCWGC 4 cut(s) 343, 515, 657, 734
ApoI RAATTY 1 cut(s) 507
ArsI GACNNNNNNTTYG 2 cut(s) 525, 557
AspLEI GCGC 2 cut(s) 311, 386
AspS9I GGNCC 4 cut(s) 133, 259, 295, 352
AsuHPI GGTGA 4 cut(s) 202, 262, 379, 904
AsuII TTCGAA 1 cut(s) 198
AvaII GGWCC 1 cut(s) 259
BalI TGGCCA 1 cut(s) 434
BanI GGYRCC 1 cut(s) 383
BauI CACGAG 1 cut(s) 362
BbrPI CACGTG 1 cut(s) 394
BbvI GCAGC 4 cut(s) 330, 527, 644, 746
BccI CCATC 2 cut(s) 700, 821
BcgI CGANNNNNNTGC 2 cut(s) 225, 259
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 2 cut(s) 165, 792
BcoDI GTCTC 5 cut(s) 129, 526, 552, 641, 990
BfaI CTAG 3 cut(s) 549, 678, 1010
BfoI RGCGCY 1 cut(s) 387
BglII AGATCT 1 cut(s) 663
BisI GCNGC 6 cut(s) 307, 344, 416, 516, 658, 735
BlsI GCNGC 6 cut(s) 308, 345, 417, 517, 659, 736
Bme1390I CCNGG 1 cut(s) 293
Bme18I GGWCC 1 cut(s) 259
BmgBI CACGTC 2 cut(s) 496, 536
BmgT120I GGNCC 4 cut(s) 133, 259, 295, 352
BmiI GGNNCC 2 cut(s) 385, 574
BmrFI CCNGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 736
BoxI GACNNNNGTC 1 cut(s) 320
BpmI CTGGAG 2 cut(s) 323, 1071
Bpu14I TTCGAA 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 665
BsaAI YACGTR 1 cut(s) 394
BsaHI GRCGYC 1 cut(s) 384
BsaI GGTCTC 1 cut(s) 641
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 2 cut(s) 238, 1094
Bse118I RCCGGY 3 cut(s) 349, 380, 411
Bse1I ACTGG 2 cut(s) 262, 1088
Bse3DI GCAATG 1 cut(s) 972
BseBI CCWGG 1 cut(s) 293
BseGI GGATG 2 cut(s) 225, 928
BseLI CCNNNNNNNGG 2 cut(s) 238, 1094
BseMI GCAATG 1 cut(s) 972
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 2 cut(s) 262, 1088
BseRI GAGGAG 1 cut(s) 326
BseXI GCAGC 4 cut(s) 330, 527, 644, 746
BseYI CCCAGC 1 cut(s) 135
Bsh1236I CGCG 1 cut(s) 309
BshFI GGCC 5 cut(s) 134, 296, 353, 434, 691
BshNI GGYRCC 1 cut(s) 383
BsiSI CCGG 3 cut(s) 350, 381, 412
BslI CCNNNNNNNGG 2 cut(s) 238, 1094
BsmAI GTCTC 5 cut(s) 129, 526, 552, 641, 990
BsmBI CGTCTC 1 cut(s) 526
BsmI GAATGC 1 cut(s) 185
BsnI GGCC 5 cut(s) 134, 296, 353, 434, 691
Bso31I GGTCTC 1 cut(s) 641
Bsp119I TTCGAA 1 cut(s) 198
Bsp143I GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
BspACI CCGC 4 cut(s) 307, 404, 415, 784
BspANI GGCC 5 cut(s) 134, 296, 353, 434, 691
BspCNI CTCAG 1 cut(s) 331
BspFNI CGCG 1 cut(s) 309
BspLI GGNNCC 2 cut(s) 385, 574
BspT104I TTCGAA 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 641
BsrDI GCAATG 1 cut(s) 972
BsrFI RCCGGY 3 cut(s) 349, 380, 411
BsrI ACTGG 2 cut(s) 262, 1088
BssAI RCCGGY 3 cut(s) 349, 380, 411
BssMI GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
BssNI GRCGYC 1 cut(s) 384
BssSI CACGAG 1 cut(s) 362
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 72, 373
BstACI GRCGYC 1 cut(s) 384
BstBAI YACGTR 1 cut(s) 394
BstBI TTCGAA 1 cut(s) 198
BstC8I GCNNGC 2 cut(s) 413, 732
BstDEI CTNAG 1 cut(s) 318
BstF5I GGATG 2 cut(s) 225, 928
BstFNI CGCG 1 cut(s) 309
BstH2I RGCGCY 1 cut(s) 387
BstHHI GCGC 2 cut(s) 311, 386
BstKTI GATC 6 cut(s) 111, 168, 666, 795, 906, 1044
BstMAI GTCTC 5 cut(s) 129, 526, 552, 641, 990
BstMBI GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
BstMWI GCNNNNNNNGC 2 cut(s) 446, 473
BstNI CCWGG 1 cut(s) 293
BstPAI GACNNNNGTC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 291
BstUI CGCG 1 cut(s) 309
BstV1I GCAGC 4 cut(s) 330, 527, 644, 746
BstX2I RGATCY 1 cut(s) 663
BstXI CCANNNNNNTGG 1 cut(s) 394
BstYI RGATCY 1 cut(s) 663
BsuRI GGCC 5 cut(s) 134, 296, 353, 434, 691
BtrI CACGTC 2 cut(s) 496, 536
BtsCI GGATG 2 cut(s) 225, 928
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 413, 732
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 2 cut(s) 311, 386
Cfr10I RCCGGY 3 cut(s) 349, 380, 411
Cfr13I GGNCC 4 cut(s) 133, 259, 295, 352
Csp6I GTAC 3 cut(s) 68, 451, 703
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviAII CATG 2 cut(s) 237, 790
CviQI GTAC 3 cut(s) 68, 451, 703
DdeI CTNAG 1 cut(s) 318
DinI GGCGCC 1 cut(s) 385
DpnI GATC 6 cut(s) 110, 167, 665, 794, 905, 1043
DpnII GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
EaeI YGGCCR 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 641
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 1 cut(s) 259
Eco57I CTGAAG 2 cut(s) 29, 377
Eco72I CACGTG 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 472
EgeI GGCGCC 1 cut(s) 385
EheI GGCGCC 1 cut(s) 385
Esp3I CGTCTC 1 cut(s) 526
FaeI CATG 2 cut(s) 240, 793
FalI AAGNNNNNCTT 4 cut(s) 679, 711, 791, 823
FatI CATG 2 cut(s) 236, 789
FauI CCCGC 1 cut(s) 791
FbaI TGATCA 2 cut(s) 165, 792
Fnu4HI GCNGC 6 cut(s) 307, 344, 416, 516, 658, 735
FokI GGATG 2 cut(s) 212, 935
Fsp4HI GCNGC 6 cut(s) 307, 344, 416, 516, 658, 735
FspBI CTAG 3 cut(s) 549, 678, 1010
GlaI GCGC 2 cut(s) 310, 385
GluI GCNGC 6 cut(s) 307, 344, 416, 516, 658, 735
GsaI CCCAGC 1 cut(s) 139
GsuI CTGGAG 2 cut(s) 323, 1071
HaeII RGCGCY 1 cut(s) 387
HaeIII GGCC 5 cut(s) 134, 296, 353, 434, 691
HapII CCGG 3 cut(s) 350, 381, 412
HhaI GCGC 2 cut(s) 311, 386
Hin1I GRCGYC 1 cut(s) 384
Hin1II CATG 2 cut(s) 240, 793
Hin6I GCGC 2 cut(s) 309, 384
HinP1I GCGC 2 cut(s) 309, 384
HincII GTYRAC 1 cut(s) 325
HindII GTYRAC 1 cut(s) 325
HindIII AAGCTT 1 cut(s) 1056
HinfI GANTC 5 cut(s) 200, 242, 316, 499, 1013
HpaII CCGG 3 cut(s) 350, 381, 412
HphI GGTGA 4 cut(s) 202, 262, 379, 904
Hpy166II GTNNAC 2 cut(s) 325, 397
Hpy188I TCNGA 5 cut(s) 205, 233, 457, 504, 908
Hpy188III TCNNGA 4 cut(s) 106, 163, 257, 1021
Hpy8I GTNNAC 2 cut(s) 325, 397
Hpy99I CGWCG 1 cut(s) 497
HpyAV CCTTC 1 cut(s) 718
HpyCH4III ACNGT 2 cut(s) 72, 373
HpyCH4IV ACGT 3 cut(s) 393, 495, 535
HpyCH4V TGCA 5 cut(s) 145, 660, 734, 767, 815
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 473
HpyF3I CTNAG 1 cut(s) 318
HpySE526I ACGT 3 cut(s) 393, 495, 535
Hsp92I GRCGYC 1 cut(s) 384
Hsp92II CATG 2 cut(s) 240, 793
HspAI GCGC 2 cut(s) 309, 384
KasI GGCGCC 1 cut(s) 383
KroI GCCGGC 1 cut(s) 411
KroNI GCCGGC 1 cut(s) 413
Ksp22I TGATCA 2 cut(s) 165, 792
Kzo9I GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 4 cut(s) 330, 527, 644, 746
LweI GCATC 1 cut(s) 736
MaeI CTAG 3 cut(s) 549, 678, 1010
MaeII ACGT 3 cut(s) 393, 495, 535
MaeIII GTNAC 2 cut(s) 313, 367
MalI GATC 6 cut(s) 110, 167, 665, 794, 905, 1043
MboI GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
MboII GAAGA 2 cut(s) 470, 1054
MflI RGATCY 1 cut(s) 663
MlsI TGGCCA 1 cut(s) 434
MluCI AATT 4 cut(s) 507, 858, 875, 1048
MluNI TGGCCA 1 cut(s) 434
Mly113I GGCGCC 1 cut(s) 384
MlyI GAGTC 2 cut(s) 310, 1022
MmeI TCCRAC 2 cut(s) 228, 586
Mox20I TGGCCA 1 cut(s) 434
MroNI GCCGGC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 434
MseI TTAA 2 cut(s) 29, 840
MslI CAYNNNNRTG 1 cut(s) 494
Msp20I TGGCCA 1 cut(s) 434
MspA1I CMGCKG 2 cut(s) 343, 518
MspI CCGG 3 cut(s) 350, 381, 412
MspR9I CCNGG 1 cut(s) 293
Mva1269I GAATGC 1 cut(s) 185
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 309
MwoI GCNNNNNNNGC 2 cut(s) 446, 473
NaeI GCCGGC 1 cut(s) 413
NarI GGCGCC 1 cut(s) 384
NdeII GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
NgoMIV GCCGGC 1 cut(s) 411
NlaIII CATG 2 cut(s) 240, 793
NlaIV GGNNCC 2 cut(s) 385, 574
NmuCI GTSAC 2 cut(s) 313, 367
NspV TTCGAA 1 cut(s) 198
PctI GAATGC 1 cut(s) 185
PdiI GCCGGC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 200, 242, 499
PflMI CCANNNNNTGG 1 cut(s) 1094
PkrI GCNGC 6 cut(s) 308, 345, 417, 517, 659, 736
PleI GAGTC 2 cut(s) 310, 1021
PluTI GGCGCC 1 cut(s) 387
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 2 cut(s) 310, 1021
Ppu21I YACGTR 1 cut(s) 394
PshAI GACNNNNGTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 291
PspCI CACGTG 1 cut(s) 394
PspFI CCCAGC 1 cut(s) 135
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 385, 574
PspPI GGNCC 4 cut(s) 133, 259, 295, 352
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 663
PvuII CAGCTG 2 cut(s) 343, 518
RsaI GTAC 3 cut(s) 69, 452, 704
RsaNI GTAC 3 cut(s) 68, 451, 703
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 2 cut(s) 29, 840
SatI GCNGC 6 cut(s) 307, 344, 416, 516, 658, 735
Sau3AI GATC 6 cut(s) 108, 165, 663, 792, 903, 1041
Sau96I GGNCC 4 cut(s) 133, 259, 295, 352
SchI GAGTC 2 cut(s) 310, 1022
ScrFI CCNGG 1 cut(s) 293
SfaNI GCATC 1 cut(s) 736
SfoI GGCGCC 1 cut(s) 385
SfuI TTCGAA 1 cut(s) 198
SgrAI CRCCGGYG 1 cut(s) 380
SinI GGWCC 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 644
SmoI CTYRAG 1 cut(s) 644
Sse9I AATT 4 cut(s) 507, 858, 875, 1048
SsiI CCGC 4 cut(s) 307, 404, 415, 784
SspDI GGCGCC 1 cut(s) 383
SspMI CTAG 3 cut(s) 549, 678, 1010
StyD4I CCNGG 1 cut(s) 291
TaaI ACNGT 2 cut(s) 72, 373
TaiI ACGT 3 cut(s) 396, 498, 538
TaqI TCGA 2 cut(s) 198, 492
TasI AATT 4 cut(s) 507, 858, 875, 1048
TauI GCSGC 2 cut(s) 309, 418
TfiI GAWTC 3 cut(s) 200, 242, 499
Tru1I TTAA 2 cut(s) 29, 840
Tru9I TTAA 2 cut(s) 29, 840
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 2 cut(s) 313, 367
TseI GCWGC 4 cut(s) 343, 515, 657, 734
Tsp45I GTSAC 2 cut(s) 313, 367
TspDTI ATGAA 4 cut(s) 178, 478, 642, 882
TspRI CASTG 1 cut(s) 66
Van91I CCANNNNNTGG 1 cut(s) 1094
VpaK11BI GGWCC 1 cut(s) 259
XapI RAATTY 1 cut(s) 507
XspI CTAG 3 cut(s) 549, 678, 1010
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.