RLG00000028405

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22362706 .. 22373590
10885 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028405

Sequence Viewer

Length: 981 bp
ATGGCAGATGGTAATCACAGTGAGCTCCCAACAGTTCTGGTACTTCGCCGATGCCCAACCATTGCTCTCTTGGAACCCATAGTCTCTCAAAAATTCAACTTACTGAAAGCCTGGGACTCTCCTCTCCCTCAAGACCAGTTCTTGACCACTCACGGCAGTTCCGTCCAGGCCCTTATCTCCTCAGCCGATGGCCCACCCATTACCGCCCAAATCCTCCACTTGCTCCCCTCCCTCAAAGTCATATCCACCACCAGCGCTAGCATTGACCATGTTGACTTGGCCGAGTGTCGACGCCGCGGCGTTGCCGTCGCCAACGCCGCTAAAGCCTTTGCTGAGGACGCTGCTGACTCGGCTGTGGGGCTGTTCATTGATGTCATGAGAAAGATTTCGGCTTCGAATCGGTATGTTAGGGATGGGCTCTGGGCTACCAGAGGAGACTATCCTCTTGGTTCTAAGATTGGAGGAAAGAAGGTCGGTATTGTTGGATTGGGCAACATTGGGTTAGAAGTTGCAAAACGATTGGAGGCATTTGGCTGCACTGTCTTGTACAATTCGAGGAGTGAAAAGCCATCTTTGTCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATAATCTGCTGTGCATTGACAGCGGAAACCCACCACATGATCAACAAGGAAGTATTGTCTGCGTTGGGAAGAGAGGGCGTGATTGTTAATGTAGTGCGCAGAGTTATTATCGATGAGAAGGAAATGGTGCAGTGTTTGGTGCGAGGAGAGATAAGGGGTGCTGGCTTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTTTGCATTGGATAATGTTGTACTGTCACTGCATCAAGCTGTCACTACCCATGAAGCTATCGTGGCTTTGGGTGAATTAGTAACAGGGAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTCAGGCTGTGATTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

327

Amino Acids

35.14

Weight (kDa)

5.65

Isoelectric Point (pI)

33.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 55 - 321 5.4e-18 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 2.7e-46 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 728
AccI GTMKAC 1 cut(s) 289
AccII CGCG 1 cut(s) 297
AciI CCGC 5 cut(s) 204, 295, 297, 318, 653
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 92, 925
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 3 cut(s) 42, 548, 858
AfeI AGCGCT 1 cut(s) 256
AgsI TTSAA 1 cut(s) 97
AjnI CCWGG 2 cut(s) 110, 165
AluBI AGCT 5 cut(s) 25, 607, 835, 875, 893
AluI AGCT 5 cut(s) 25, 607, 835, 875, 893
Alw21I GWGCWC 2 cut(s) 27, 837
Alw26I GTCTC 2 cut(s) 88, 429
Aor51HI AGCGCT 1 cut(s) 256
AoxI GGCC 3 cut(s) 168, 190, 279
ApeKI GCWGC 3 cut(s) 341, 534, 611
ApoI RAATTY 2 cut(s) 92, 925
Asp700I GAANNNNTTC 1 cut(s) 385
AspLEI GCGC 2 cut(s) 257, 729
AspS9I GGNCC 2 cut(s) 169, 191
AsuHPI GGTGA 1 cut(s) 920
AsuII TTCGAA 1 cut(s) 395
AsuNHI GCTAGC 1 cut(s) 257
BanII GRGCYC 3 cut(s) 27, 420, 837
Bbv12I GWGCWC 2 cut(s) 27, 837
BbvCI CCTCAGC 2 cut(s) 181, 333
BbvI GCAGC 3 cut(s) 328, 521, 623
BccI CCATC 4 cut(s) 2, 182, 407, 577
BceAI ACGGC 2 cut(s) 169, 290
BciT130I CCWGG 2 cut(s) 112, 167
BclI TGATCA 1 cut(s) 669
BcoDI GTCTC 2 cut(s) 88, 429
BfaI CTAG 1 cut(s) 258
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 6 cut(s) 295, 298, 318, 342, 535, 612
BlsI GCNGC 6 cut(s) 296, 299, 319, 343, 536, 613
Bme1390I CCNGG 2 cut(s) 112, 167
BmgT120I GGNCC 2 cut(s) 169, 191
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 2 cut(s) 112, 167
BmsI GCATC 3 cut(s) 41, 613, 877
BmtI GCTAGC 1 cut(s) 261
Bpu10I CCTNAGC 2 cut(s) 181, 333
Bpu14I TTCGAA 1 cut(s) 395
BpuEI CTTGAG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 111, 295
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 2 cut(s) 112, 167
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 2 cut(s) 418, 805
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 3 cut(s) 195, 324, 977
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 5 cut(s) 111, 169, 447, 571, 789
BseXI GCAGC 3 cut(s) 328, 521, 623
BsgI GTGCAG 2 cut(s) 520, 779
Bsh1236I CGCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 170, 192, 281
BshVI ATCGAT 1 cut(s) 741
BsiHKAI GWGCWC 2 cut(s) 27, 837
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 2 cut(s) 88, 429
BsmFI GGGAC 1 cut(s) 128
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 3 cut(s) 170, 192, 281
Bsp119I TTCGAA 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 27, 420, 837
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 1 cut(s) 669
BspACI CCGC 5 cut(s) 204, 295, 297, 318, 653
BspANI GGCC 3 cut(s) 170, 192, 281
BspCNI CTCAG 3 cut(s) 194, 325, 976
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 1 cut(s) 297
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 75
BspOI GCTAGC 1 cut(s) 261
BspT104I TTCGAA 1 cut(s) 395
BsrDI GCAATG 1 cut(s) 60
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 1 cut(s) 669
BssNI GRCGYC 1 cut(s) 292
Bst2UI CCWGG 2 cut(s) 112, 167
Bst4CI ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 395
BstC8I GCNNGC 3 cut(s) 259, 609, 793
BstDEI CTNAG 4 cut(s) 181, 333, 453, 963
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 2 cut(s) 418, 805
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 257, 729
BstKTI GATC 1 cut(s) 672
BstMAI GTCTC 2 cut(s) 88, 429
BstMBI GATC 1 cut(s) 669
BstMWI GCNNNNNNNGC 6 cut(s) 317, 323, 338, 350, 650, 899
BstNI CCWGG 2 cut(s) 112, 167
BstSCI CCNGG 2 cut(s) 110, 165
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 3 cut(s) 328, 521, 623
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 3 cut(s) 170, 192, 281
BsuTUI ATCGAT 1 cut(s) 741
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 2 cut(s) 418, 805
BtsI GCAGTG 2 cut(s) 767, 863
BtsIMutI CAGTG 5 cut(s) 25, 537, 625, 767, 863
Cac8I GCNNGC 3 cut(s) 259, 609, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 257, 729
Cfr13I GGNCC 2 cut(s) 169, 191
Cfr42I CCGCGG 1 cut(s) 298
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 2 cut(s) 300, 347
Csp6I GTAC 3 cut(s) 41, 547, 857
CviAII CATG 4 cut(s) 269, 376, 667, 887
CviQI GTAC 3 cut(s) 41, 547, 857
DdeI CTNAG 4 cut(s) 181, 333, 453, 963
DpnI GATC 1 cut(s) 671
DpnII GATC 1 cut(s) 669
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Ecl136II GAGCTC 2 cut(s) 25, 835
Eco24I GRGCYC 3 cut(s) 27, 420, 837
Eco47III AGCGCT 1 cut(s) 256
Eco53kI GAGCTC 2 cut(s) 25, 835
EcoICRI GAGCTC 2 cut(s) 25, 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 110, 165
EcoT38I GRGCYC 3 cut(s) 27, 420, 837
FaeI CATG 4 cut(s) 272, 379, 670, 890
FaiI YATR 9 cut(s) 80, 242, 270, 377, 405, 580, 632, 668, 888
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 268, 375, 666, 886
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 6 cut(s) 295, 298, 318, 342, 535, 612
FokI GGATG 2 cut(s) 425, 812
FriOI GRGCYC 3 cut(s) 27, 420, 837
Fsp4HI GCNGC 6 cut(s) 295, 298, 318, 342, 535, 612
FspBI CTAG 1 cut(s) 258
FspI TGCGCA 1 cut(s) 728
GlaI GCGC 2 cut(s) 256, 728
GluI GCNGC 6 cut(s) 295, 298, 318, 342, 535, 612
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 3 cut(s) 170, 192, 281
HgaI GACGC 2 cut(s) 300, 347
HhaI GCGC 2 cut(s) 257, 729
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 4 cut(s) 272, 379, 670, 890
Hin6I GCGC 2 cut(s) 255, 727
HinP1I GCGC 2 cut(s) 255, 727
HincII GTYRAC 2 cut(s) 274, 290
HindII GTYRAC 2 cut(s) 274, 290
HinfI GANTC 3 cut(s) 116, 347, 397
HphI GGTGA 1 cut(s) 920
Hpy166II GTNNAC 2 cut(s) 274, 290
Hpy188III TCNNGA 3 cut(s) 131, 142, 376
Hpy8I GTNNAC 2 cut(s) 274, 290
Hpy99I CGWCG 2 cut(s) 294, 311
HpyAV CCTTC 2 cut(s) 463, 742
HpyCH4III ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
HpyCH4V TGCA 7 cut(s) 512, 537, 611, 644, 760, 842, 868
HpyF10VI GCNNNNNNNGC 6 cut(s) 317, 323, 338, 350, 650, 899
HpyF3I CTNAG 4 cut(s) 181, 333, 453, 963
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 4 cut(s) 272, 379, 670, 890
HspAI GCGC 2 cut(s) 255, 727
Ksp22I TGATCA 1 cut(s) 669
KspI CCGCGG 1 cut(s) 298
Kzo9I GATC 1 cut(s) 669
LmnI GCTCC 2 cut(s) 30, 228
Lsp1109I GCAGC 3 cut(s) 328, 521, 623
LweI GCATC 3 cut(s) 41, 613, 877
MaeI CTAG 1 cut(s) 258
MaeIII GTNAC 3 cut(s) 861, 877, 916
MalI GATC 1 cut(s) 671
MboI GATC 1 cut(s) 669
MboII GAAGA 2 cut(s) 711, 931
MhlI GDGCHC 3 cut(s) 27, 420, 837
MluCI AATT 5 cut(s) 92, 550, 584, 911, 925
MlyI GAGTC 2 cut(s) 110, 341
MmeI TCCRAC 1 cut(s) 463
MroXI GAANNNNTTC 1 cut(s) 385
MseI TTAA 1 cut(s) 717
MspA1I CMGCKG 2 cut(s) 297, 653
MspR9I CCNGG 2 cut(s) 112, 167
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 2 cut(s) 112, 167
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 6 cut(s) 317, 323, 338, 350, 650, 899
NdeII GATC 1 cut(s) 669
NheI GCTAGC 1 cut(s) 257
NlaIII CATG 4 cut(s) 272, 379, 670, 890
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 2 cut(s) 307, 329
NmuCI GTSAC 2 cut(s) 861, 877
NsbI TGCGCA 1 cut(s) 728
NspV TTCGAA 1 cut(s) 395
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 935
PdmI GAANNNNTTC 1 cut(s) 385
PfeI GAWTC 1 cut(s) 397
PkrI GCNGC 6 cut(s) 296, 299, 319, 343, 536, 613
PleI GAGTC 2 cut(s) 110, 341
PpsI GAGTC 2 cut(s) 110, 341
Psp124BI GAGCTC 2 cut(s) 27, 837
Psp6I CCWGG 2 cut(s) 110, 165
PspGI CCWGG 2 cut(s) 110, 165
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 2 cut(s) 169, 191
RsaI GTAC 3 cut(s) 42, 548, 858
RsaNI GTAC 3 cut(s) 41, 547, 857
SacI GAGCTC 2 cut(s) 27, 837
SacII CCGCGG 1 cut(s) 298
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 6 cut(s) 295, 298, 318, 342, 535, 612
Sau3AI GATC 1 cut(s) 669
Sau96I GGNCC 2 cut(s) 169, 191
SchI GAGTC 2 cut(s) 110, 341
ScrFI CCNGG 2 cut(s) 112, 167
SduI GDGCHC 3 cut(s) 27, 420, 837
SetI ASST 6 cut(s) 27, 474, 609, 837, 877, 895
SfaNI GCATC 3 cut(s) 41, 613, 877
Sfr303I CCGCGG 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 395
SgrBI CCGCGG 1 cut(s) 298
SmlI CTYRAG 1 cut(s) 129
SmoI CTYRAG 1 cut(s) 129
Sse9I AATT 5 cut(s) 92, 550, 584, 911, 925
SsiI CCGC 5 cut(s) 204, 295, 297, 318, 653
SspMI CTAG 1 cut(s) 258
SstI GAGCTC 2 cut(s) 27, 837
StyD4I CCNGG 2 cut(s) 110, 165
TaaI ACNGT 6 cut(s) 20, 34, 541, 620, 861, 954
TaqI TCGA 4 cut(s) 289, 395, 554, 741
TasI AATT 5 cut(s) 92, 550, 584, 911, 925
TatI WGTACW 2 cut(s) 546, 856
TauI GCSGC 3 cut(s) 297, 300, 320
TfiI GAWTC 1 cut(s) 397
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 5 cut(s) 25, 544, 625, 767, 870
TseFI GTSAC 2 cut(s) 861, 877
TseI GCWGC 3 cut(s) 341, 534, 611
Tsp45I GTSAC 2 cut(s) 861, 877
TspDTI ATGAA 2 cut(s) 355, 903
TspGWI ACGGA 1 cut(s) 151
TspRI CASTG 5 cut(s) 25, 544, 625, 767, 870
XapI RAATTY 2 cut(s) 92, 925
XcmI CCANNNNNNNNNTGG 1 cut(s) 67
XmiI GTMKAC 1 cut(s) 289
XmnI GAANNNNTTC 1 cut(s) 385
XspI CTAG 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.