RLG00000028404

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000446
Physical Location & Seq
Reverse (-)
22306750 .. 22308360
1611 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028404

Sequence Viewer

Length: 981 bp
ATGGCAGATGGTAATCACAGTGAGCCCCCTACAGTTCTGGTACTTCGCCGCTCCCCAACCATTGCTCTCTTGGAACCCATAATCTCCCAAAAATTCAACTTACTGAAAGCCTGGGACTCTCCTCTCCCACAAGACCAGTTCTTGACCACCCACGCCCGCTCCGTCCAGGCCCTCCTCTCCTCCGTCGGTGGGCTGCCCATCACCACCCAAATCCTCCACTTGCTCCCTTCCCTCAAATTCATAGCCACCACCAGCGCCGGCATGGACCACGTTGACTCGGCCGAGTGTCGACGCCTCGGCGTTGCTGTCGCCAACGCCGCTAAAGCCTTTGCTGAGGACGCTGCTGACATGGCTGTTGGGCTGTTCATTGATGTCATGAGAAAGCTGTCGGCTTCGGATCGGTATGTTGGGGACGGGCTCTGGGCTACCAGAGGAAACTATCCTCTTGGTTCGAAGATTGGAGGAAAGAAGGTTGGTATTGTTGGATTGGGGAACATTGGTTTAGAAGTTGCAAAAAGATTGGAGGCATTTGGCTGCAGTGTCTTGTACAATTCAAGGAGCGAAAAGTCATCTTTGTCATACCAATTTTATTCCAATGTGTGTGAGCTTGCAGCAGACAGTGATGCTCTCATCATCTGTTGTGCATTGACAGAGCAAACCCACCACATGATCAACAGGGAAGTTTTGTCCGCGTTGGGAAGAGAGGGGGTTATCGTTAATGTAGGGCGCGGAGCTATTATCGATGAGAAAGAAATGGTTGAGTATTTGGTGCGAGGAGAGATTGGAGGTGCTGGCCTGGATGTGTTTGAGAATGAGCCTGATGTTCCTAAAGAGCTCTATGCATTGGATAATGTTGTACTGTCACCGCATCAAGCTGTCGCTACACATGAAGCTATCGTGGCTTTGGGTGAAGTAGTAACAGGCAATTTGGAAGCATTCTTCTCAAACAAACCGTTGCTTTCTTCGGCTGTGATTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

327

Amino Acids

34.84

Weight (kDa)

5.31

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 34 - 321 6.4e-19 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 120 - 292 2.7e-50 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 51, 159
AccI GTMKAC 1 cut(s) 289
AccII CGCG 2 cut(s) 692, 729
AciI CCGC 6 cut(s) 49, 157, 318, 690, 729, 866
AclWI GGATC 1 cut(s) 405
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 2 cut(s) 92, 236
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 3 cut(s) 42, 548, 858
AfiI CCNNNNNNNGG 1 cut(s) 189
AgsI TTSAA 2 cut(s) 97, 555
AjnI CCWGG 3 cut(s) 110, 165, 795
AluBI AGCT 6 cut(s) 385, 607, 734, 835, 875, 893
AluI AGCT 6 cut(s) 385, 607, 734, 835, 875, 893
Alw21I GWGCWC 1 cut(s) 837
AlwI GGATC 1 cut(s) 405
AoxI GGCC 3 cut(s) 168, 279, 793
ApeKI GCWGC 4 cut(s) 193, 341, 534, 611
ApoI RAATTY 2 cut(s) 92, 236
AseI ATTAAT 1 cut(s) 975
AspLEI GCGC 2 cut(s) 257, 729
AspS9I GGNCC 2 cut(s) 169, 265
AsuHPI GGTGA 3 cut(s) 193, 855, 920
AsuII TTCGAA 1 cut(s) 452
AvaII GGWCC 1 cut(s) 265
BanII GRGCYC 3 cut(s) 27, 420, 837
Bbv12I GWGCWC 1 cut(s) 837
BbvCI CCTCAGC 1 cut(s) 333
BbvI GCAGC 4 cut(s) 180, 328, 521, 623
BccI CCATC 2 cut(s) 2, 206
BciT130I CCWGG 3 cut(s) 112, 167, 797
BclI TGATCA 1 cut(s) 669
BfmI CTRYAG 2 cut(s) 30, 535
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 6 cut(s) 49, 194, 318, 342, 535, 612
BlsI GCNGC 6 cut(s) 50, 195, 319, 343, 536, 613
Bme1390I CCNGG 3 cut(s) 112, 167, 797
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 2 cut(s) 169, 265
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 3 cut(s) 112, 167, 797
BmsI GCATC 2 cut(s) 613, 877
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 1 cut(s) 452
Bsa29I ATCGAT 1 cut(s) 741
BsaBI GATNNNNATC 1 cut(s) 12
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 2 cut(s) 111, 295
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 189
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 60
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 3 cut(s) 112, 167, 797
BseCI ATCGAT 1 cut(s) 741
BseDI CCNNGG 2 cut(s) 111, 295
BseGI GGATG 1 cut(s) 805
BseJI GATNNNNATC 1 cut(s) 12
BseLI CCNNNNNNNGG 1 cut(s) 189
BseMI GCAATG 1 cut(s) 60
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 1 cut(s) 136
BseRI GAGGAG 4 cut(s) 111, 164, 169, 789
BseX3I CGGCCG 1 cut(s) 279
BseXI GCAGC 4 cut(s) 180, 328, 521, 623
Bsh1236I CGCG 2 cut(s) 692, 729
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 3 cut(s) 170, 281, 795
BshVI ATCGAT 1 cut(s) 741
BsiEI CGRYCG 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 1 cut(s) 258
BslFI GGGAC 2 cut(s) 128, 425
BslI CCNNNNNNNGG 1 cut(s) 189
BsmFI GGGAC 2 cut(s) 128, 425
BsmI GAATGC 1 cut(s) 935
BsnI GGCC 3 cut(s) 170, 281, 795
Bsp119I TTCGAA 1 cut(s) 452
Bsp1286I GDGCHC 3 cut(s) 27, 420, 837
Bsp1407I TGTACA 1 cut(s) 546
Bsp143I GATC 2 cut(s) 397, 669
BspACI CCGC 6 cut(s) 49, 157, 318, 690, 729, 866
BspANI GGCC 3 cut(s) 170, 281, 795
BspCNI CTCAG 1 cut(s) 325
BspDI ATCGAT 1 cut(s) 741
BspFNI CGCG 2 cut(s) 692, 729
BspHI TCATGA 1 cut(s) 375
BspLI GGNNCC 1 cut(s) 75
BspMAI CTGCAG 1 cut(s) 539
BspPI GGATC 1 cut(s) 405
BspT104I TTCGAA 1 cut(s) 452
BsrBI CCGCTC 2 cut(s) 51, 159
BsrDI GCAATG 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 257
BsrGI TGTACA 1 cut(s) 546
BsrI ACTGG 1 cut(s) 136
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 2 cut(s) 111, 295
BssMI GATC 2 cut(s) 397, 669
BssNI GRCGYC 1 cut(s) 292
Bst2UI CCWGG 3 cut(s) 112, 167, 797
Bst4CI ACNGT 5 cut(s) 20, 34, 620, 861, 954
Bst6I CTCTTC 1 cut(s) 694
BstACI GRCGYC 1 cut(s) 292
BstAUI TGTACA 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 4 cut(s) 157, 259, 609, 793
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 1 cut(s) 805
BstFNI CGCG 2 cut(s) 692, 729
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 257, 729
BstKTI GATC 2 cut(s) 400, 672
BstMBI GATC 2 cut(s) 397, 669
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 5 cut(s) 317, 323, 338, 350, 899
BstNI CCWGG 3 cut(s) 112, 167, 797
BstSCI CCNGG 3 cut(s) 110, 165, 795
BstSFI CTRYAG 2 cut(s) 30, 535
BstUI CGCG 2 cut(s) 692, 729
BstV1I GCAGC 4 cut(s) 180, 328, 521, 623
BstZI CGGCCG 1 cut(s) 279
Bsu15I ATCGAT 1 cut(s) 741
BsuRI GGCC 3 cut(s) 170, 281, 795
BsuTUI ATCGAT 1 cut(s) 741
BtsCI GGATG 1 cut(s) 805
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 3 cut(s) 25, 544, 625
Cac8I GCNNGC 4 cut(s) 157, 259, 609, 793
CciI TCATGA 1 cut(s) 375
CfoI GCGC 2 cut(s) 257, 729
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 169, 265
ClaI ATCGAT 1 cut(s) 741
CseI GACGC 2 cut(s) 300, 347
Csp6I GTAC 3 cut(s) 41, 547, 857
CviAII CATG 5 cut(s) 262, 349, 376, 667, 887
CviQI GTAC 3 cut(s) 41, 547, 857
DdeI CTNAG 1 cut(s) 333
DpnI GATC 2 cut(s) 399, 671
DpnII GATC 2 cut(s) 397, 669
EaeI YGGCCR 1 cut(s) 279
EagI CGGCCG 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 694
EarI CTCTTC 1 cut(s) 694
Ecl136II GAGCTC 1 cut(s) 835
EclXI CGGCCG 1 cut(s) 279
Eco24I GRGCYC 3 cut(s) 27, 420, 837
Eco47I GGWCC 1 cut(s) 265
Eco52I CGGCCG 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 835
EcoICRI GAGCTC 1 cut(s) 835
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 3 cut(s) 110, 165, 795
EcoT22I ATGCAT 1 cut(s) 844
EcoT38I GRGCYC 3 cut(s) 27, 420, 837
FaeI CATG 5 cut(s) 265, 352, 379, 670, 890
FaqI GGGAC 2 cut(s) 128, 425
FatI CATG 5 cut(s) 261, 348, 375, 666, 886
FauI CCCGC 1 cut(s) 164
FbaI TGATCA 1 cut(s) 669
FblI GTMKAC 1 cut(s) 289
Fnu4HI GCNGC 6 cut(s) 49, 194, 318, 342, 535, 612
FokI GGATG 1 cut(s) 812
FriOI GRGCYC 3 cut(s) 27, 420, 837
Fsp4HI GCNGC 6 cut(s) 49, 194, 318, 342, 535, 612
GlaI GCGC 2 cut(s) 256, 728
GluI GCNGC 6 cut(s) 49, 194, 318, 342, 535, 612
HaeII RGCGCY 1 cut(s) 258
HaeIII GGCC 3 cut(s) 170, 281, 795
HapII CCGG 1 cut(s) 258
HgaI GACGC 2 cut(s) 300, 347
HhaI GCGC 2 cut(s) 257, 729
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 5 cut(s) 265, 352, 379, 670, 890
Hin6I GCGC 2 cut(s) 255, 727
HinP1I GCGC 2 cut(s) 255, 727
HincII GTYRAC 2 cut(s) 274, 290
HindII GTYRAC 2 cut(s) 274, 290
HinfI GANTC 2 cut(s) 116, 275
HpaII CCGG 1 cut(s) 258
HphI GGTGA 3 cut(s) 193, 855, 920
Hpy166II GTNNAC 2 cut(s) 274, 290
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 2 cut(s) 142, 376
Hpy8I GTNNAC 2 cut(s) 274, 290
Hpy99I CGWCG 2 cut(s) 188, 294
HpyAV CCTTC 2 cut(s) 237, 463
HpyCH4III ACNGT 5 cut(s) 20, 34, 620, 861, 954
HpyCH4IV ACGT 1 cut(s) 270
HpyCH4V TGCA 5 cut(s) 512, 537, 611, 644, 842
HpyF10VI GCNNNNNNNGC 5 cut(s) 317, 323, 338, 350, 899
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 270
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 5 cut(s) 265, 352, 379, 670, 890
HspAI GCGC 2 cut(s) 255, 727
KroI GCCGGC 1 cut(s) 257
KroNI GCCGGC 1 cut(s) 259
Ksp22I TGATCA 1 cut(s) 669
Kzo9I GATC 2 cut(s) 397, 669
LmnI GCTCC 5 cut(s) 56, 164, 228, 558, 731
Lsp1109I GCAGC 4 cut(s) 180, 328, 521, 623
LweI GCATC 2 cut(s) 613, 877
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 2 cut(s) 861, 916
MalI GATC 2 cut(s) 399, 671
MbiI CCGCTC 2 cut(s) 51, 159
MboI GATC 2 cut(s) 397, 669
MboII GAAGA 4 cut(s) 466, 711, 931, 954
MhlI GDGCHC 3 cut(s) 27, 420, 837
MluCI AATT 6 cut(s) 92, 236, 550, 584, 925, 976
MlyI GAGTC 2 cut(s) 110, 269
MmeI TCCRAC 1 cut(s) 463
Mph1103I ATGCAT 1 cut(s) 844
MroNI GCCGGC 1 cut(s) 257
MseI TTAA 2 cut(s) 717, 975
MspI CCGG 1 cut(s) 258
MspR9I CCNGG 3 cut(s) 112, 167, 797
Mva1269I GAATGC 1 cut(s) 935
MvaI CCWGG 3 cut(s) 112, 167, 797
MvnI CGCG 2 cut(s) 692, 729
MwoI GCNNNNNNNGC 5 cut(s) 317, 323, 338, 350, 899
NaeI GCCGGC 1 cut(s) 259
NdeII GATC 2 cut(s) 397, 669
NgoMIV GCCGGC 1 cut(s) 257
NlaIII CATG 5 cut(s) 265, 352, 379, 670, 890
NlaIV GGNNCC 1 cut(s) 75
NmeAIII GCCGAG 3 cut(s) 257, 276, 307
NmuCI GTSAC 1 cut(s) 861
NsiI ATGCAT 1 cut(s) 844
NspV TTCGAA 1 cut(s) 452
PagI TCATGA 1 cut(s) 375
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 1 cut(s) 935
PdiI GCCGGC 1 cut(s) 259
PkrI GCNGC 6 cut(s) 50, 195, 319, 343, 536, 613
PleI GAGTC 2 cut(s) 110, 269
PpsI GAGTC 2 cut(s) 110, 269
PshBI ATTAAT 1 cut(s) 975
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 3 cut(s) 110, 165, 795
PspGI CCWGG 3 cut(s) 110, 165, 795
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 2 cut(s) 169, 265
PstI CTGCAG 1 cut(s) 539
RsaI GTAC 3 cut(s) 42, 548, 858
RsaNI GTAC 3 cut(s) 41, 547, 857
SacI GAGCTC 1 cut(s) 837
SalI GTCGAC 1 cut(s) 288
SaqAI TTAA 2 cut(s) 717, 975
SatI GCNGC 6 cut(s) 49, 194, 318, 342, 535, 612
Sau3AI GATC 2 cut(s) 397, 669
Sau96I GGNCC 2 cut(s) 169, 265
SchI GAGTC 2 cut(s) 110, 269
ScrFI CCNGG 3 cut(s) 112, 167, 797
SduI GDGCHC 3 cut(s) 27, 420, 837
SetI ASST 9 cut(s) 273, 387, 474, 609, 736, 790, 837, 877, 895
SfaNI GCATC 2 cut(s) 613, 877
SfcI CTRYAG 2 cut(s) 30, 535
SfuI TTCGAA 1 cut(s) 452
SinI GGWCC 1 cut(s) 265
Sse9I AATT 6 cut(s) 92, 236, 550, 584, 925, 976
SsiI CCGC 6 cut(s) 49, 157, 318, 690, 729, 866
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 3 cut(s) 110, 165, 795
TaaI ACNGT 5 cut(s) 20, 34, 620, 861, 954
TaiI ACGT 1 cut(s) 273
TaqI TCGA 3 cut(s) 289, 452, 741
TasI AATT 6 cut(s) 92, 236, 550, 584, 925, 976
TatI WGTACW 2 cut(s) 546, 856
TauI GCSGC 2 cut(s) 51, 320
Tru1I TTAA 2 cut(s) 717, 975
Tru9I TTAA 2 cut(s) 717, 975
TscAI CASTG 3 cut(s) 25, 544, 625
TseFI GTSAC 1 cut(s) 861
TseI GCWGC 4 cut(s) 193, 341, 534, 611
Tsp45I GTSAC 1 cut(s) 861
TspDTI ATGAA 3 cut(s) 229, 355, 903
TspGWI ACGGA 2 cut(s) 151, 172
TspRI CASTG 3 cut(s) 25, 544, 625
VpaK11BI GGWCC 1 cut(s) 265
VspI ATTAAT 1 cut(s) 975
XapI RAATTY 2 cut(s) 92, 236
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 259
XmiI GTMKAC 1 cut(s) 289
Zsp2I ATGCAT 1 cut(s) 844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.