Rw1G020330

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000446
Physical Location & Seq
Forward (+)
43976711 .. 43979665
2955 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020330.1

Sequence Viewer

Length: 1050 bp
ATGGAGTTCTGTTTGCAGATTCCAACCAGGCAGGAAGCTAAAATGGCCAGATTGCAATTCCGTGATGCAAATGAGGATGATGTACCAACCGAGGAACTCCCACATCTCCTACTCATTCATCCACCATCATCCTTCACACGTCTCCCATCTGAAATCTGTGAAAAGTTCCGTGTCCTGAAAGCATGGGAGTCGCCACTCCCTCTAGACCAGTTCCTGATCAGCCATGCACGCTCAATCCAAGCCCTACTCATTCGCGGTTTTCTTATGATTAACACAGACATCCTTAAGCAGCTACCGGCACTGAAACTCATCATAACAATCAGCGTTGGTGTGAACCACATTGACATGGCAGAGTGCCGGCGGCGTGGGATTTCTGTGACCAATGCGGGAAGTGTATACTCAGAAGATGTTGCTGATATTGCTGTTGGGTTGTACATTGATGTGCTTAGAAAACTTTCAGCAGCAGACCGGTATGTAAGACAAGGGCTTTGGAGTAGCAATGGAAATTACCCTCTTGGTTCCAAGTTAGGTGGGAAGCGAGTTGGGATTGTTGGATTAGGAAGCATTGGCTCAGAAGTTGCTAAACGACTTGAGGCCTTTGGCTGCATCATCTCATACAATTCAAGGAGGGAAAAGCCAACTTCGTCATACCCCTTCTATTCTATCATATCTGAACTTGCAGCCAATAGTGATGCCCTCATCATCTGTTGTGCGTTAACAGAACAAACCCACCATCTGATTAACAAGGAAGTCTTGGCTTCACTGGGAAGAGAGGGTGTGATTGTTAATGTAGGCCGCGGGGCAATAATTGATGAGAAGGAACTGGTGAAGTGTTTGGTGAGTGGGGAAATAAGAGGTGCCGGTTTTGATGTGTTTGAGAATGAGCCTCAAGTTCCTGAAGAGCTCTTTACATTGGACAATGTTGTTCTATCCCCACATACGGCTATTTTTACTCCGGAATCTTTGCAGAGCGTGAAGGAAGTACTAATCGCCAATGTGGAAGCTTTCTTCTTGAATAAACCTCTAGTTACTCCTGTCATGGATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00260 ko00630 ko01100 ko01110 ko01130 ko01200 map00260 map00630 map01100 map01110 map01130 map01200
Pfam Domains
Protein Families
2-Hacid_dh 2-Hacid_dh_C

Protein Analysis

349

Amino Acids

38.54

Weight (kDa)

5.52

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 70 - 346 9e-21 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 143 - 315 1e-51 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000446)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12550 AT2G45630 AT2G45630
fragaria_vesca FvH4_7g12570 FvH4_7g12570 FvH4_7g12580 FvH4_7g12581 FvH4_7g12581 FvH4_7g12590 FvH4_7g12590
malus_domestica MD07G1124400.v1.1 MD07G1124500.v1.1
prunus_persica Prupe.2G149900_v2.0.a1 Prupe.2G149900_v2.0.a1 Prupe.2G150000_v2.0.a1 Prupe.2G150200_v2.0.a1 Prupe.2G150300_v2.0.a1 Prupe.2G150500_v2.0.a1 Prupe.2G151500_v2.0.a1 Prupe.2G151800_v2.0.a1
pyrus_communis pycom02g16040 pycom07g11720 pycom07g11750
rosa_chinensis RchiOBHm_Chr1g0351471 RchiOBHm_Chr1g0351501 RchiOBHm_Chr1g0351521 RchiOBHm_Chr1g0351531 RchiOBHm_Chr1g0351551 RchiOBHm_Chr1g0351561 RchiOBHm_Chr1g0351571 RchiOBHm_Chr6g0297861
rosa_laevigata RLG00000011555 RLG00000028400 RLG00000028401 RLG00000028402 RLG00000028403 RLG00000028404 RLG00000028405 RLG00000028406
rosa_multiflora Rmu_sc0001144.1_g000033 Rmu_sc0001144.1_g000038 Rmu_sc0001144.1_g000039 Rmu_sc0001144.1_g000040 Rmu_sc0003137.1_g000016 Rmu_sc0006343.1_g000010 Rmu_sc0025960.1_g000001 Rmu_ssc0000403.1_g000015
rosa_roxburghii Rroxscaffold_4G00303720 Rroxscaffold_4G00303730 Rroxscaffold_4G00303740 Rroxscaffold_4G00303750 Rroxscaffold_4G00303770 Rroxscaffold_4G00303780 Rroxscaffold_7G00170140 Rroxscaffold_7G00178990
rosa_rugosa Rorug01G0218300 Rorug01G0218400 Rorug01G0218400 Rorug01G0218500 Rorug01G0218800 Rorug06G0276100
rosa_samantha Rh1AG232900 Rh1AG233000 Rh1AG233200 Rh1AG233300 Rh1AG233400 Rh1AG233500 Rh1BG203800 Rh1BG203900 Rh1BG204000 Rh1BG204100 Rh1BG204200 Rh1CG217600 Rh1CG217800 Rh1CG217900 Rh1CG218000 Rh1CG218100 Rh1DG230700 Rh1DG230800 Rh1DG230900 Rh1DG231000 Rh6AG386700 Rh6BG395300 Rh6CG401100 Rh6DG387400
rosa_wichuraiana Rw1G020270 Rw1G020300 Rw1G020310 Rw1G020320 Rw1G020330 Rw1G020340 Rw1G020350 Rw6G033700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 857
AccI GTMKAC 1 cut(s) 396
AccII CGCG 2 cut(s) 255, 798
AccIII TCCGGA 1 cut(s) 955
AciI CCGC 5 cut(s) 255, 361, 386, 796, 798
AcoI YGGCCR 1 cut(s) 45
AcuI CTGAAG 1 cut(s) 918
AfaI GTAC 3 cut(s) 84, 434, 984
AfiI CCNNNNNNNGG 1 cut(s) 940
AflII CTTAAG 1 cut(s) 284
AflIII ACRYGT 1 cut(s) 137
AgeI ACCGGT 1 cut(s) 468
AgsI TTSAA 2 cut(s) 624, 1015
AjiI CACGTC 1 cut(s) 140
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 4 cut(s) 38, 292, 904, 1004
AluI AGCT 4 cut(s) 38, 292, 904, 1004
Alw21I GWGCWC 1 cut(s) 906
Alw26I GTCTC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 214
Aor13HI TCCGGA 1 cut(s) 955
AoxI GGCC 3 cut(s) 45, 594, 793
ApeKI GCWGC 4 cut(s) 289, 461, 603, 680
ArsI GACNNNNNNTTYG 2 cut(s) 471, 503
AsiGI ACCGGT 1 cut(s) 468
Asp700I GAANNNNTTC 1 cut(s) 454
AsuHPI GGTGA 2 cut(s) 838, 850
BalI TGGCCA 1 cut(s) 47
BanI GGYRCC 1 cut(s) 857
BanII GRGCYC 1 cut(s) 906
Bbv12I GWGCWC 1 cut(s) 906
BbvI GCAGC 4 cut(s) 301, 473, 590, 692
BccI CCATC 3 cut(s) 133, 154, 741
BceAI ACGGC 1 cut(s) 957
BcgI CGANNNNNNTGC 2 cut(s) 171, 205
BciT130I CCWGG 1 cut(s) 28
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 1 cut(s) 146
BfaI CTAG 2 cut(s) 203, 1025
BfrI CTTAAG 1 cut(s) 284
BisI GCNGC 6 cut(s) 290, 362, 462, 604, 681, 796
BlsI GCNGC 6 cut(s) 291, 363, 463, 605, 682, 797
BmcAI AGTACT 1 cut(s) 984
Bme1390I CCNGG 1 cut(s) 28
BmgBI CACGTC 1 cut(s) 140
BmiI GGNNCC 2 cut(s) 520, 859
BmrFI CCNGG 1 cut(s) 28
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 3 cut(s) 55, 615, 682
BmuI ACTGGG 1 cut(s) 773
BpuEI CTTGAG 2 cut(s) 611, 873
BsaJI CCNNGG 2 cut(s) 90, 796
BsaWI WCCGGW 2 cut(s) 468, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 940
Bse118I RCCGGY 4 cut(s) 295, 357, 468, 860
Bse1I ACTGG 3 cut(s) 208, 768, 828
Bse3DI GCAATG 1 cut(s) 505
BseAI TCCGGA 1 cut(s) 955
BseBI CCWGG 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 90, 796
BseGI GGATG 5 cut(s) 82, 118, 128, 279, 1048
BseLI CCNNNNNNNGG 1 cut(s) 940
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 2 cut(s) 414, 585
BseNI ACTGG 3 cut(s) 208, 768, 828
BseXI GCAGC 4 cut(s) 301, 473, 590, 692
Bsh1236I CGCG 2 cut(s) 255, 798
BshFI GGCC 3 cut(s) 47, 596, 795
BshNI GGYRCC 1 cut(s) 857
BshTI ACCGGT 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 906
BsiSI CCGG 5 cut(s) 296, 358, 469, 861, 956
BslI CCNNNNNNNGG 1 cut(s) 940
BsmAI GTCTC 1 cut(s) 146
BsmBI CGTCTC 1 cut(s) 146
BsnI GGCC 3 cut(s) 47, 596, 795
Bsp1286I GDGCHC 1 cut(s) 906
Bsp13I TCCGGA 1 cut(s) 955
Bsp1407I TGTACA 1 cut(s) 432
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 5 cut(s) 255, 361, 386, 796, 798
BspANI GGCC 3 cut(s) 47, 596, 795
BspCNI CTCAG 2 cut(s) 413, 584
BspEI TCCGGA 1 cut(s) 955
BspFNI CGCG 2 cut(s) 255, 798
BspLI GGNNCC 2 cut(s) 520, 859
BspQI GCTCTTC 1 cut(s) 894
BspT107I GGYRCC 1 cut(s) 857
BspTI CTTAAG 1 cut(s) 284
BsrDI GCAATG 1 cut(s) 505
BsrFI RCCGGY 4 cut(s) 295, 357, 468, 860
BsrGI TGTACA 1 cut(s) 432
BsrI ACTGG 3 cut(s) 208, 768, 828
BssAI RCCGGY 4 cut(s) 295, 357, 468, 860
BssECI CCNNGG 2 cut(s) 90, 796
BssMI GATC 1 cut(s) 216
BssNAI GTATAC 1 cut(s) 397
Bst1107I GTATAC 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 28
Bst6I CTCTTC 2 cut(s) 763, 894
BstAFI CTTAAG 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 432
BstC8I GCNNGC 2 cut(s) 229, 359
BstDEI CTNAG 3 cut(s) 400, 446, 571
BstDSI CCRYGG 1 cut(s) 796
BstF5I GGATG 5 cut(s) 82, 118, 128, 279, 1048
BstFNI CGCG 2 cut(s) 255, 798
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 146
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 3 cut(s) 44, 228, 419
BstNI CCWGG 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 26
BstUI CGCG 2 cut(s) 255, 798
BstV1I GCAGC 4 cut(s) 301, 473, 590, 692
BstZ17I GTATAC 1 cut(s) 397
BsuRI GGCC 3 cut(s) 47, 596, 795
BtgI CCRYGG 1 cut(s) 796
BtrI CACGTC 1 cut(s) 140
BtsCI GGATG 5 cut(s) 82, 118, 128, 279, 1048
BtsIMutI CAGTG 2 cut(s) 299, 761
Cac8I GCNNGC 2 cut(s) 229, 359
CaiI CAGNNNCTG 1 cut(s) 214
Cfr10I RCCGGY 4 cut(s) 295, 357, 468, 860
Cfr42I CCGCGG 1 cut(s) 799
Csp6I GTAC 3 cut(s) 83, 433, 983
CspAI ACCGGT 1 cut(s) 468
CspCI CAANNNNNGTGG 2 cut(s) 511, 546
CviAII CATG 4 cut(s) 183, 224, 346, 1039
CviQI GTAC 3 cut(s) 83, 433, 983
DdeI CTNAG 3 cut(s) 400, 446, 571
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 2 cut(s) 763, 894
EarI CTCTTC 2 cut(s) 763, 894
Ecl136II GAGCTC 1 cut(s) 904
Eco147I AGGCCT 1 cut(s) 596
Eco24I GRGCYC 1 cut(s) 906
Eco53kI GAGCTC 1 cut(s) 904
Eco57I CTGAAG 1 cut(s) 918
EcoICRI GAGCTC 1 cut(s) 904
EcoRII CCWGG 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 906
Esp3I CGTCTC 1 cut(s) 146
FaeI CATG 4 cut(s) 186, 227, 349, 1042
FalI AAGNNNNNCTT 2 cut(s) 737, 769
FatI CATG 4 cut(s) 182, 223, 345, 1038
FauI CCCGC 2 cut(s) 379, 791
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 1 cut(s) 396
Fnu4HI GCNGC 6 cut(s) 290, 362, 462, 604, 681, 796
FokI GGATG 4 cut(s) 89, 105, 115, 266
FriOI GRGCYC 1 cut(s) 906
Fsp4HI GCNGC 6 cut(s) 290, 362, 462, 604, 681, 796
FspBI CTAG 2 cut(s) 203, 1025
GluI GCNGC 6 cut(s) 290, 362, 462, 604, 681, 796
HaeIII GGCC 3 cut(s) 47, 596, 795
HapII CCGG 5 cut(s) 296, 358, 469, 861, 956
Hin1II CATG 4 cut(s) 186, 227, 349, 1042
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 3 cut(s) 19, 188, 959
HpaI GTTAAC 1 cut(s) 717
HpaII CCGG 5 cut(s) 296, 358, 469, 861, 956
HphI GGTGA 2 cut(s) 838, 850
Hpy166II GTNNAC 3 cut(s) 334, 397, 717
Hpy188I TCNGA 5 cut(s) 151, 403, 574, 673, 738
Hpy188III TCNNGA 6 cut(s) 175, 203, 214, 896, 956, 1012
Hpy8I GTNNAC 3 cut(s) 334, 397, 717
HpyAV CCTTC 4 cut(s) 142, 664, 811, 970
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 7 cut(s) 16, 55, 68, 227, 606, 680, 967
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 228, 419
HpyF3I CTNAG 3 cut(s) 400, 446, 571
HpySE526I ACGT 1 cut(s) 139
Hsp92II CATG 4 cut(s) 186, 227, 349, 1042
Kpn2I TCCGGA 1 cut(s) 955
KroI GCCGGC 1 cut(s) 357
KroNI GCCGGC 1 cut(s) 359
Ksp22I TGATCA 1 cut(s) 216
KspAI GTTAAC 1 cut(s) 717
KspI CCGCGG 1 cut(s) 799
Kzo9I GATC 1 cut(s) 216
LguI GCTCTTC 1 cut(s) 894
Lsp1109I GCAGC 4 cut(s) 301, 473, 590, 692
LweI GCATC 3 cut(s) 55, 615, 682
MaeI CTAG 2 cut(s) 203, 1025
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 2 cut(s) 376, 1027
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 4 cut(s) 416, 780, 911, 1000
MhlI GDGCHC 1 cut(s) 906
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 4 cut(s) 56, 505, 619, 807
MluNI TGGCCA 1 cut(s) 47
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 2 cut(s) 47, 532
Mox20I TGGCCA 1 cut(s) 47
MroI TCCGGA 1 cut(s) 955
MroNI GCCGGC 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 454
MscI TGGCCA 1 cut(s) 47
MseI TTAA 5 cut(s) 270, 285, 716, 741, 786
MslI CAYNNNNRTG 2 cut(s) 344, 440
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 798
MspCI CTTAAG 1 cut(s) 284
MspI CCGG 5 cut(s) 296, 358, 469, 861, 956
MspR9I CCNGG 1 cut(s) 28
MvaI CCWGG 1 cut(s) 28
MvnI CGCG 2 cut(s) 255, 798
MwoI GCNNNNNNNGC 3 cut(s) 44, 228, 419
NaeI GCCGGC 1 cut(s) 359
NdeII GATC 1 cut(s) 216
NgoMIV GCCGGC 1 cut(s) 357
NlaIII CATG 4 cut(s) 186, 227, 349, 1042
NlaIV GGNNCC 2 cut(s) 520, 859
NmuCI GTSAC 1 cut(s) 376
PceI AGGCCT 1 cut(s) 596
PciSI GCTCTTC 1 cut(s) 894
PdiI GCCGGC 1 cut(s) 359
PdmI GAANNNNTTC 1 cut(s) 454
PfeI GAWTC 2 cut(s) 19, 959
PinAI ACCGGT 1 cut(s) 468
PkrI GCNGC 6 cut(s) 291, 363, 463, 605, 682, 797
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
Psp124BI GAGCTC 1 cut(s) 906
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 520, 859
PstNI CAGNNNCTG 1 cut(s) 214
RsaI GTAC 3 cut(s) 84, 434, 984
RsaNI GTAC 3 cut(s) 83, 433, 983
RseI CAYNNNNRTG 2 cut(s) 344, 440
SacI GAGCTC 1 cut(s) 906
SacII CCGCGG 1 cut(s) 799
SapI GCTCTTC 1 cut(s) 894
SaqAI TTAA 5 cut(s) 270, 285, 716, 741, 786
SatI GCNGC 6 cut(s) 290, 362, 462, 604, 681, 796
Sau3AI GATC 1 cut(s) 216
ScaI AGTACT 1 cut(s) 984
SchI GAGTC 1 cut(s) 197
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 906
SetI ASST 8 cut(s) 40, 142, 294, 532, 859, 906, 1006, 1024
SfaNI GCATC 3 cut(s) 55, 615, 682
Sfr303I CCGCGG 1 cut(s) 799
SgrBI CCGCGG 1 cut(s) 799
SmiMI CAYNNNNRTG 2 cut(s) 344, 440
SmlI CTYRAG 3 cut(s) 284, 590, 888
SmoI CTYRAG 3 cut(s) 284, 590, 888
Sse9I AATT 4 cut(s) 56, 505, 619, 807
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 5 cut(s) 255, 361, 386, 796, 798
SspMI CTAG 2 cut(s) 203, 1025
SstI GAGCTC 1 cut(s) 906
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 26
TaiI ACGT 1 cut(s) 142
TasI AATT 4 cut(s) 56, 505, 619, 807
TatI WGTACW 2 cut(s) 432, 982
TauI GCSGC 2 cut(s) 364, 798
TfiI GAWTC 2 cut(s) 19, 959
Tru1I TTAA 5 cut(s) 270, 285, 716, 741, 786
Tru9I TTAA 5 cut(s) 270, 285, 716, 741, 786
TscAI CASTG 2 cut(s) 306, 768
TseFI GTSAC 1 cut(s) 376
TseI GCWGC 4 cut(s) 289, 461, 603, 680
Tsp45I GTSAC 1 cut(s) 376
TspDTI ATGAA 1 cut(s) 107
TspGWI ACGGA 2 cut(s) 50, 158
TspRI CASTG 2 cut(s) 306, 768
Vha464I CTTAAG 1 cut(s) 284
XbaI TCTAGA 1 cut(s) 202
XmiI GTMKAC 1 cut(s) 396
XmnI GAANNNNTTC 1 cut(s) 454
XspI CTAG 2 cut(s) 203, 1025
ZrmI AGTACT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.