AT4G32375

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000579
Physical Location & Seq
Forward (+)
15629115 .. 15631690
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32375.1

Sequence Viewer

Length: 1272 bp
ATGGTAAGGGAGTGGGGAAACTACGAAAGTAAATGTTGGTTATGTTTCACAAACGTGAAAGGTCTCGTCCTCAATGGATCTGGGATTCTCCATCCCCATGGTGAAGCTTGGTGGTCCTCTATTGAGCACTCCCACCGACCACGAACAATAGGATTTAGTGGAAGTAGTAACATCTTATATAATGGGCTCACTCAGATGAATAGCCCAAAGAATCACATTTCTATTTTAGATTGCACCAACGTAACTTTATCAAACTTCCATCTAATCGCACCGAAAGATAGTCCAAATACTGATGGCATCGATATTGCTCATTCAAACAATATCCGAATTTTCAACTCATCTATCCAAACCGGTGATGATTGTATTGCGATTAATGGTGGTTCGTATGATATCAACATAACTCATGTAGCATGTGGTCCAGGTCATGGCATCAGTATTGGAAGTTTGGGGAGATATAGTGTAAACGATACGGTCCAGAACGTAAAAATAAGACACTGCAGCTTCAATGGAACAGAAAATGGAGCAAGAATAAAGACTTGGACTGGAGGTCTCGGGGTCGCCAAAAATATATTATACGAGAATATAACATTAACAGATACCAAATATCCTATCATCATCGATCAACATTACTGTAATGGTGGCCACAATTGTACAAAAGAGGCGATGACTGCTGTGAAAGTGAGCAATGTGACATTTAGATACTTTACAGGAACATGTGCAAATGATATTGCGATAAAACTAGATTGTGATGAAGTAACGGGTTGTAAAGATATCGTGATGGAACACATCAACATCACTTCTTCATCTACGAAAAGGCCTCTAACCGCATATTGTCAATTTGCGGATATAATTAGTCACTTTGTCTCCATGAAAATCAAATGTGATTATTATGAAGAACCTTTAGTACCAGTTGAACCTCCACAACAGGTTGAACCTCCAACGCCGACTAAACCTCTGGCTCCAGCTAAACCTCCACGACACGTTGGACCTCTAATGCCGACTAAACCTCCAACCATGTTTCCTAAACCTCTGGCACCAGCTAAATCTCCACGACACGTTGAACTTCCAATGCCGACTAAACCTCCAACCATGTTTCCTAAACCTCTGGCTCCAGCTAAACCTCCACGACACGTTGAACCTCCAATGCCGACTAAACCTCCAACCATGTTTCCTAAACCATTGGCACCAGCTAAACCTCCTGTATACTATGCTAAACCTCCAGCGCCGAATGCTCAACCTTCGATGTTGTCTTTTCTTTCGTGCCTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

423

Amino Acids

46.36

Weight (kDa)

8.74

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 9 - 271 7.7e-79 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1033, 1183
AccB7I CCANNNNNTGG 1 cut(s) 425
AccI GTMKAC 1 cut(s) 1203
AciI CCGC 2 cut(s) 825, 842
AclWI GGATC 1 cut(s) 85
AcoI YGGCCR 1 cut(s) 640
AcsI RAATTY 1 cut(s) 327
AfaI GTAC 2 cut(s) 652, 906
AfiI CCNNNNNNNGG 1 cut(s) 425
AflIII ACRYGT 4 cut(s) 713, 979, 1054, 1129
AgeI ACCGGT 1 cut(s) 350
AgsI TTSAA 7 cut(s) 315, 334, 505, 914, 932, 1061, 1136
AhdI GACNNNNNGTC 1 cut(s) 546
AjnI CCWGG 1 cut(s) 418
AjuI GAANNNNNNNTTGG 2 cut(s) 520, 552
AleI CACNNNNGTG 1 cut(s) 53
AluBI AGCT 6 cut(s) 107, 501, 965, 1040, 1115, 1190
AluI AGCT 6 cut(s) 107, 501, 965, 1040, 1115, 1190
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 3 cut(s) 68, 554, 868
AlwI GGATC 1 cut(s) 85
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 2 cut(s) 640, 815
ApeKI GCWGC 1 cut(s) 498
ApoI RAATTY 1 cut(s) 327
AseI ATTAAT 1 cut(s) 372
AsiGI ACCGGT 1 cut(s) 350
AspLEI GCGC 1 cut(s) 1225
AspS9I GGNCC 4 cut(s) 114, 416, 472, 986
AsuHPI GGTGA 2 cut(s) 113, 365
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 4 cut(s) 114, 416, 472, 986
BalI TGGCCA 1 cut(s) 642
BanI GGYRCC 2 cut(s) 1033, 1183
BanII GRGCYC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 510
BccI CCATC 4 cut(s) 99, 267, 287, 772
BciT130I CCWGG 1 cut(s) 420
BcoDI GTCTC 3 cut(s) 68, 554, 868
BfaI CTAG 1 cut(s) 740
BfmI CTRYAG 1 cut(s) 496
BfoI RGCGCY 1 cut(s) 1226
BisI GCNGC 1 cut(s) 499
BlsI GCNGC 1 cut(s) 500
Bme1390I CCNGG 1 cut(s) 420
Bme18I GGWCC 4 cut(s) 114, 416, 472, 986
BmeRI GACNNNNNGTC 1 cut(s) 546
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 114, 416, 472, 986
BmiI GGNNCC 4 cut(s) 960, 1035, 1110, 1185
BmrFI CCNGG 1 cut(s) 420
BmsI GCATC 2 cut(s) 306, 438
BpmI CTGGAG 4 cut(s) 564, 945, 1095, 1203
Bsa29I ATCGAT 2 cut(s) 300, 618
BsaI GGTCTC 2 cut(s) 68, 554
BsaJI CCNNGG 1 cut(s) 97
BsaWI WCCGGW 1 cut(s) 350
Bsc4I CCNNNNNNNGG 1 cut(s) 425
Bse118I RCCGGY 1 cut(s) 350
Bse1I ACTGG 2 cut(s) 547, 908
Bse3DI GCAATG 1 cut(s) 691
BseBI CCWGG 1 cut(s) 420
BseCI ATCGAT 2 cut(s) 300, 618
BseDI CCNNGG 1 cut(s) 97
BseGI GGATG 1 cut(s) 91
BseLI CCNNNNNNNGG 1 cut(s) 425
BseMI GCAATG 1 cut(s) 691
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 2 cut(s) 547, 908
BseXI GCAGC 1 cut(s) 510
BshFI GGCC 2 cut(s) 642, 817
BshNI GGYRCC 2 cut(s) 1033, 1183
BshTI ACCGGT 1 cut(s) 350
BshVI ATCGAT 2 cut(s) 300, 618
BsiHKAI GWGCWC 1 cut(s) 129
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 1 cut(s) 351
BslI CCNNNNNNNGG 1 cut(s) 425
BsmAI GTCTC 3 cut(s) 68, 554, 868
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 2 cut(s) 642, 817
Bso31I GGTCTC 2 cut(s) 68, 554
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 2 cut(s) 129, 189
Bsp1407I TGTACA 1 cut(s) 650
Bsp143I GATC 2 cut(s) 77, 619
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 2 cut(s) 825, 842
BspANI GGCC 2 cut(s) 642, 817
BspCNI CTCAG 1 cut(s) 205
BspDI ATCGAT 2 cut(s) 300, 618
BspLI GGNNCC 4 cut(s) 960, 1035, 1110, 1185
BspMAI CTGCAG 1 cut(s) 500
BspPI GGATC 1 cut(s) 85
BspT107I GGYRCC 2 cut(s) 1033, 1183
BspTNI GGTCTC 2 cut(s) 68, 554
BsrDI GCAATG 1 cut(s) 691
BsrFI RCCGGY 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 650
BsrI ACTGG 2 cut(s) 547, 908
BssAI RCCGGY 1 cut(s) 350
BssECI CCNNGG 1 cut(s) 97
BssMI GATC 2 cut(s) 77, 619
BssNAI GTATAC 1 cut(s) 1204
BssT1I CCWWGG 1 cut(s) 97
Bst1107I GTATAC 1 cut(s) 1204
Bst2UI CCWGG 1 cut(s) 420
Bst4CI ACNGT 2 cut(s) 472, 632
BstAUI TGTACA 1 cut(s) 650
BstDEI CTNAG 1 cut(s) 192
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 1226
BstHHI GCGC 1 cut(s) 1225
BstKTI GATC 2 cut(s) 80, 622
BstMAI GTCTC 3 cut(s) 68, 554, 868
BstMBI GATC 2 cut(s) 77, 619
BstMWI GCNNNNNNNGC 2 cut(s) 668, 1229
BstNI CCWGG 1 cut(s) 420
BstNSI RCATGY 2 cut(s) 414, 717
BstSCI CCNGG 1 cut(s) 418
BstSFI CTRYAG 1 cut(s) 496
BstV1I GCAGC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 77
BstXI CCANNNNNNTGG 1 cut(s) 98
BstYI RGATCY 1 cut(s) 77
BstZ17I GTATAC 1 cut(s) 1204
Bsu15I ATCGAT 2 cut(s) 300, 618
BsuRI GGCC 2 cut(s) 642, 817
BsuTUI ATCGAT 2 cut(s) 300, 618
BtgI CCRYGG 1 cut(s) 97
BtgZI GCGATG 1 cut(s) 677
BtsCI GGATG 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 1 cut(s) 493
CfoI GCGC 1 cut(s) 1225
Cfr10I RCCGGY 1 cut(s) 350
Cfr13I GGNCC 4 cut(s) 114, 416, 472, 986
ClaI ATCGAT 2 cut(s) 300, 618
Csp6I GTAC 2 cut(s) 651, 905
CspAI ACCGGT 1 cut(s) 350
CviAII CATG 9 cut(s) 98, 404, 411, 425, 714, 868, 1015, 1090, 1165
CviQI GTAC 2 cut(s) 651, 905
DdeI CTNAG 1 cut(s) 192
DpnI GATC 2 cut(s) 79, 621
DpnII GATC 2 cut(s) 77, 619
DriI GACNNNNNGTC 1 cut(s) 546
EaeI YGGCCR 1 cut(s) 640
Eam1105I GACNNNNNGTC 1 cut(s) 546
Eco130I CCWWGG 1 cut(s) 97
Eco147I AGGCCT 1 cut(s) 817
Eco24I GRGCYC 1 cut(s) 189
Eco31I GGTCTC 2 cut(s) 68, 554
Eco32I GATATC 2 cut(s) 391, 772
Eco47I GGWCC 4 cut(s) 114, 416, 472, 986
Eco88I CYCGRG 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 418
EcoRV GATATC 2 cut(s) 391, 772
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 97
FaeI CATG 9 cut(s) 101, 407, 414, 428, 717, 871, 1018, 1093, 1168
FatI CATG 9 cut(s) 97, 403, 410, 424, 713, 867, 1014, 1089, 1164
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 1 cut(s) 499
FokI GGATG 1 cut(s) 78
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 1 cut(s) 499
FspBI CTAG 1 cut(s) 740
GlaI GCGC 1 cut(s) 1224
GluI GCNGC 1 cut(s) 499
GsuI CTGGAG 4 cut(s) 564, 945, 1095, 1203
HaeII RGCGCY 1 cut(s) 1226
HaeIII GGCC 2 cut(s) 642, 817
HapII CCGG 1 cut(s) 351
HhaI GCGC 1 cut(s) 1225
Hin1II CATG 9 cut(s) 101, 407, 414, 428, 717, 871, 1018, 1093, 1168
Hin6I GCGC 1 cut(s) 1223
HinP1I GCGC 1 cut(s) 1223
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 2 cut(s) 85, 211
HpaII CCGG 1 cut(s) 351
HphI GGTGA 2 cut(s) 113, 365
Hpy166II GTNNAC 2 cut(s) 463, 1204
Hpy188I TCNGA 2 cut(s) 195, 326
Hpy188III TCNNGA 2 cut(s) 475, 775
Hpy8I GTNNAC 2 cut(s) 463, 1204
HpyAV CCTTC 1 cut(s) 1248
HpyCH4III ACNGT 2 cut(s) 472, 632
HpyCH4IV ACGT 6 cut(s) 54, 240, 480, 981, 1056, 1131
HpyCH4V TGCA 3 cut(s) 234, 498, 719
HpyF10VI GCNNNNNNNGC 2 cut(s) 668, 1229
HpyF3I CTNAG 1 cut(s) 192
HpySE526I ACGT 6 cut(s) 54, 240, 480, 981, 1056, 1131
Hsp92II CATG 9 cut(s) 101, 407, 414, 428, 717, 871, 1018, 1093, 1168
HspAI GCGC 1 cut(s) 1223
Kzo9I GATC 2 cut(s) 77, 619
LmnI GCTCC 3 cut(s) 521, 964, 1114
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 2 cut(s) 306, 438
MaeI CTAG 1 cut(s) 740
MaeII ACGT 6 cut(s) 54, 240, 480, 981, 1056, 1131
MaeIII GTNAC 5 cut(s) 167, 241, 688, 754, 854
MalI GATC 2 cut(s) 79, 621
MboI GATC 2 cut(s) 77, 619
MboII GAAGA 2 cut(s) 792, 905
MfeI CAATTG 1 cut(s) 646
MflI RGATCY 1 cut(s) 77
MhlI GDGCHC 2 cut(s) 129, 189
MlsI TGGCCA 1 cut(s) 642
MluCI AATT 4 cut(s) 327, 646, 836, 849
MluNI TGGCCA 1 cut(s) 642
MmeI TCCRAC 5 cut(s) 962, 964, 1034, 1109, 1184
Mox20I TGGCCA 1 cut(s) 642
MscI TGGCCA 1 cut(s) 642
MseI TTAA 3 cut(s) 372, 590, 1270
MslI CAYNNNNRTG 3 cut(s) 53, 96, 194
Msp20I TGGCCA 1 cut(s) 642
MspI CCGG 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 420
MunI CAATTG 1 cut(s) 646
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 1 cut(s) 420
MwoI GCNNNNNNNGC 2 cut(s) 668, 1229
NcoI CCATGG 1 cut(s) 97
NdeII GATC 2 cut(s) 77, 619
NlaIII CATG 9 cut(s) 101, 407, 414, 428, 717, 871, 1018, 1093, 1168
NlaIV GGNNCC 4 cut(s) 960, 1035, 1110, 1185
NmuCI GTSAC 2 cut(s) 688, 854
NspI RCATGY 2 cut(s) 414, 717
OliI CACNNNNGTG 1 cut(s) 53
PceI AGGCCT 1 cut(s) 817
PciI ACATGT 1 cut(s) 713
PctI GAATGC 1 cut(s) 1234
PfeI GAWTC 2 cut(s) 85, 211
PflMI CCANNNNNTGG 1 cut(s) 425
PinAI ACCGGT 1 cut(s) 350
PkrI GCNGC 1 cut(s) 500
PscI ACATGT 1 cut(s) 713
PshBI ATTAAT 1 cut(s) 372
Psp6I CCWGG 1 cut(s) 418
PspGI CCWGG 1 cut(s) 418
PspN4I GGNNCC 4 cut(s) 960, 1035, 1110, 1185
PspPI GGNCC 4 cut(s) 114, 416, 472, 986
PsrI GAACNNNNNNTAC 2 cut(s) 888, 920
PstI CTGCAG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 77
RsaI GTAC 2 cut(s) 652, 906
RsaNI GTAC 2 cut(s) 651, 905
RseI CAYNNNNRTG 3 cut(s) 53, 96, 194
SaqAI TTAA 3 cut(s) 372, 590, 1270
SatI GCNGC 1 cut(s) 499
Sau3AI GATC 2 cut(s) 77, 619
Sau96I GGNCC 4 cut(s) 114, 416, 472, 986
ScrFI CCNGG 1 cut(s) 420
SduI GDGCHC 2 cut(s) 129, 189
SfaNI GCATC 2 cut(s) 306, 438
SfcI CTRYAG 1 cut(s) 496
SinI GGWCC 4 cut(s) 114, 416, 472, 986
SmiMI CAYNNNNRTG 3 cut(s) 53, 96, 194
Sse9I AATT 4 cut(s) 327, 646, 836, 849
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 2 cut(s) 825, 842
SspMI CTAG 1 cut(s) 740
StuI AGGCCT 1 cut(s) 817
StyD4I CCNGG 1 cut(s) 418
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 2 cut(s) 472, 632
TaiI ACGT 6 cut(s) 57, 243, 483, 984, 1059, 1134
TaqI TCGA 3 cut(s) 300, 618, 1241
TasI AATT 4 cut(s) 327, 646, 836, 849
TatI WGTACW 1 cut(s) 650
TfiI GAWTC 2 cut(s) 85, 211
Tru1I TTAA 3 cut(s) 372, 590, 1270
Tru9I TTAA 3 cut(s) 372, 590, 1270
TscAI CASTG 1 cut(s) 500
TseFI GTSAC 2 cut(s) 688, 854
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 2 cut(s) 688, 854
TspDTI ATGAA 5 cut(s) 212, 765, 792, 884, 906
TspRI CASTG 1 cut(s) 500
Van91I CCANNNNNTGG 1 cut(s) 425
VpaK11BI GGWCC 4 cut(s) 114, 416, 472, 986
VspI ATTAAT 1 cut(s) 372
XapI RAATTY 1 cut(s) 327
XceI RCATGY 2 cut(s) 414, 717
XmiI GTMKAC 1 cut(s) 1203
XspI CTAG 1 cut(s) 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.