RchiOBHm_Chr3g0458021

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000579
Physical Location & Seq
Forward (+)
6888613 .. 6890638
2026 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42473

Sequence Viewer

Length: 1059 bp
ATGCAGTACATACTTCGACATTGTATATGGAAAAAAACATATGATGTTCTTAGTTATGGTGCTGTTGGAGATGGAAACGCTGATGATTCAAAAGTGTTGGGGAAGGTGGTGGCACCCAAAACACCGGATGCTTGGAAACAGTGTGAATCAAATTACTGGCTTTCCTTTTCATATGTGGCAAACCTCAGAATGAATGGCGGTTCAGGAATAATCGACGGCCAGGGTTCATCTTGGTGGAGCAACGCTAACGAACAAAAGCTCCACATAGATGACAAGAAGAAGTGTCAACGACCAAAGGCTCTACACTTCCATGGATGTCATAATCTTCTACTAACTGGACTTACTCATGTAAATAGTCCGAAAGGTCATATTAGTATAAGCAACTGCAGATATGTATATGTCGCCAATCTCACTATAACTGCACCTGAAGAGAGTCCAAACACCGATGGAATTGACATCTCAAACTCAAATCATGTCAACATTCATGCCTCTTATATTGGAACCGGTGATGATTGTATAGCTATCAATTCTGGATGTTCTAATATCAACATTACCAACATTGCATGTGGCCCAGGCCATGGAATTAGCGTGGGAAGCTTGGGAGAAAATGGGGCTTACGCAACAGTAGAAAATGTATCTGTGAAAAATTGTAATTTCAGTAGAACTCAAAATGGAGTAAGAATCAAGACATGGCAGGGTGGATCTGGGTACGCCAAGAATATAACCTTTGAACAAATCACACTTCATGCCACTAAAAACCCAATTATCATCGACCAATATTATTGTAATGGAGCTCACAATTGTAAAAACAAGACCTCAGCTGTCAAGGTGAGTGATGTGAGATACAGGAATATCCAAGGCACTTCAGCAAAAAAGGAGGCGATAAGATTAGACTGCAATCAGTTTTCGGGTTGTCGCAACATTGTAATGGAGCACATCAACATCACCTCGAATGTTCCTGGTAATAAGGGGATACAGGCATCATGCTACAACATCAATGGAATTTCTAAAAGCACAGTGCCGAGAGTACTTTGCCTGCAGTCCAAACTGGTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

352

Amino Acids

38.4

Weight (kDa)

8.94

Isoelectric Point (pI)

35.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 47 - 333 1.9e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 112
AccB7I CCANNNNNTGG 1 cut(s) 578
AciI CCGC 1 cut(s) 198
AclWI GGATC 1 cut(s) 709
AcoI YGGCCR 1 cut(s) 217
AcsI RAATTY 1 cut(s) 1002
AcuI CTGAAG 2 cut(s) 447, 849
AfaI GTAC 3 cut(s) 8, 710, 1029
AfiI CCNNNNNNNGG 1 cut(s) 578
AgeI ACCGGT 1 cut(s) 503
AgsI TTSAA 2 cut(s) 90, 731
AjnI CCWGG 3 cut(s) 219, 571, 958
AloI GAACNNNNNNTCC 2 cut(s) 243, 275
AluBI AGCT 5 cut(s) 259, 521, 597, 794, 821
AluI AGCT 5 cut(s) 259, 521, 597, 794, 821
Alw21I GWGCWC 2 cut(s) 796, 936
AlwI GGATC 1 cut(s) 709
AoxI GGCC 3 cut(s) 217, 568, 574
ApoI RAATTY 1 cut(s) 1002
AsiGI ACCGGT 1 cut(s) 503
AspS9I GGNCC 2 cut(s) 569, 1051
AsuHPI GGTGA 3 cut(s) 518, 841, 937
AvaII GGWCC 1 cut(s) 1051
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 796
Bbv12I GWGCWC 2 cut(s) 796, 936
BbvCI CCTCAGC 1 cut(s) 817
BccI CCATC 2 cut(s) 65, 440
BceAI ACGGC 1 cut(s) 232
BciT130I CCWGG 3 cut(s) 221, 573, 960
BciVI GTATCC 1 cut(s) 966
BfmI CTRYAG 2 cut(s) 385, 1037
BfuI GTATCC 1 cut(s) 966
BmcAI AGTACT 1 cut(s) 1029
Bme1390I CCNGG 3 cut(s) 221, 573, 960
Bme18I GGWCC 1 cut(s) 1051
BmgT120I GGNCC 2 cut(s) 569, 1051
BmiI GGNNCC 2 cut(s) 114, 502
BmrFI CCNGG 3 cut(s) 221, 573, 960
BmsI GCATC 2 cut(s) 118, 989
Bpu10I CCTNAGC 1 cut(s) 817
BsaJI CCNNGG 5 cut(s) 220, 310, 571, 577, 856
BsaWI WCCGGW 2 cut(s) 124, 503
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 4 cut(s) 161, 340, 1053, 1054
Bse3DI GCAATG 1 cut(s) 558
BseBI CCWGG 3 cut(s) 221, 573, 960
BseDI CCNNGG 5 cut(s) 220, 310, 571, 577, 856
BseGI GGATG 3 cut(s) 133, 320, 539
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 199, 831
BseNI ACTGG 4 cut(s) 161, 340, 1053, 1054
BsgI GTGCAG 1 cut(s) 405
BshFI GGCC 3 cut(s) 219, 570, 576
BshNI GGYRCC 1 cut(s) 112
BshTI ACCGGT 1 cut(s) 503
BsiHKAI GWGCWC 2 cut(s) 796, 936
BsiSI CCGG 2 cut(s) 125, 504
BslI CCNNNNNNNGG 1 cut(s) 578
BsnI GGCC 3 cut(s) 219, 570, 576
Bsp1286I GDGCHC 2 cut(s) 796, 936
Bsp143I GATC 1 cut(s) 701
Bsp19I CCATGG 2 cut(s) 310, 577
BspACI CCGC 1 cut(s) 198
BspANI GGCC 3 cut(s) 219, 570, 576
BspCNI CTCAG 2 cut(s) 198, 830
BspLI GGNNCC 2 cut(s) 114, 502
BspMAI CTGCAG 2 cut(s) 389, 1041
BspPI GGATC 1 cut(s) 709
BspT107I GGYRCC 1 cut(s) 112
BsrDI GCAATG 1 cut(s) 558
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 4 cut(s) 161, 340, 1053, 1054
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 5 cut(s) 220, 310, 571, 577, 856
BssMI GATC 1 cut(s) 701
BssT1I CCWWGG 3 cut(s) 310, 577, 856
Bst2UI CCWGG 3 cut(s) 221, 573, 960
Bst4CI ACNGT 3 cut(s) 141, 625, 1018
Bst6I CTCTTC 1 cut(s) 423
BstC8I GCNNGC 1 cut(s) 1037
BstDEI CTNAG 3 cut(s) 50, 185, 817
BstDSI CCRYGG 2 cut(s) 310, 577
BstF5I GGATG 3 cut(s) 133, 320, 539
BstKTI GATC 1 cut(s) 704
BstMBI GATC 1 cut(s) 701
BstMWI GCNNNNNNNGC 1 cut(s) 594
BstNI CCWGG 3 cut(s) 221, 573, 960
BstNSI RCATGY 1 cut(s) 567
BstSCI CCNGG 3 cut(s) 219, 571, 958
BstSFI CTRYAG 2 cut(s) 385, 1037
BstX2I RGATCY 1 cut(s) 701
BstYI RGATCY 1 cut(s) 701
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 3 cut(s) 219, 570, 576
BtgI CCRYGG 2 cut(s) 310, 577
BtsCI GGATG 3 cut(s) 133, 320, 539
BtsIMutI CAGTG 2 cut(s) 146, 1023
Cac8I GCNNGC 1 cut(s) 1037
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 2 cut(s) 569, 1051
Csp6I GTAC 3 cut(s) 7, 709, 1028
CspAI ACCGGT 1 cut(s) 503
CviAII CATG 9 cut(s) 311, 347, 473, 485, 564, 578, 690, 746, 984
CviQI GTAC 3 cut(s) 7, 709, 1028
DdeI CTNAG 3 cut(s) 50, 185, 817
DpnI GATC 1 cut(s) 703
DpnII GATC 1 cut(s) 701
EaeI YGGCCR 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 423
EarI CTCTTC 1 cut(s) 423
Ecl136II GAGCTC 1 cut(s) 794
Eco130I CCWWGG 3 cut(s) 310, 577, 856
Eco24I GRGCYC 1 cut(s) 796
Eco47I GGWCC 1 cut(s) 1051
Eco53kI GAGCTC 1 cut(s) 794
Eco57I CTGAAG 2 cut(s) 447, 849
EcoICRI GAGCTC 1 cut(s) 794
EcoRII CCWGG 3 cut(s) 219, 571, 958
EcoT14I CCWWGG 3 cut(s) 310, 577, 856
EcoT38I GRGCYC 1 cut(s) 796
ErhI CCWWGG 3 cut(s) 310, 577, 856
FaeI CATG 9 cut(s) 314, 350, 476, 488, 567, 581, 693, 749, 987
FatI CATG 9 cut(s) 310, 346, 472, 484, 563, 577, 689, 745, 983
FauNDI CATATG 2 cut(s) 40, 172
FokI GGATG 3 cut(s) 140, 327, 546
FriOI GRGCYC 1 cut(s) 796
HaeIII GGCC 3 cut(s) 219, 570, 576
HapII CCGG 2 cut(s) 125, 504
Hin1II CATG 9 cut(s) 314, 350, 476, 488, 567, 581, 693, 749, 987
HincII GTYRAC 2 cut(s) 287, 478
HindII GTYRAC 2 cut(s) 287, 478
HindIII AAGCTT 1 cut(s) 595
HinfI GANTC 4 cut(s) 86, 146, 433, 681
HpaII CCGG 2 cut(s) 125, 504
HphI GGTGA 3 cut(s) 518, 841, 937
Hpy166II GTNNAC 2 cut(s) 287, 478
Hpy188I TCNGA 2 cut(s) 188, 360
Hpy188III TCNNGA 3 cut(s) 204, 531, 685
Hpy8I GTNNAC 2 cut(s) 287, 478
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 3 cut(s) 141, 625, 1018
HpyCH4V TGCA 6 cut(s) 4, 387, 422, 563, 897, 1039
HpyF10VI GCNNNNNNNGC 1 cut(s) 594
HpyF3I CTNAG 3 cut(s) 50, 185, 817
Hsp92II CATG 9 cut(s) 314, 350, 476, 488, 567, 581, 693, 749, 987
Kzo9I GATC 1 cut(s) 701
LmnI GCTCC 4 cut(s) 237, 264, 791, 931
LweI GCATC 2 cut(s) 118, 989
MalI GATC 1 cut(s) 703
MboI GATC 1 cut(s) 701
MboII GAAGA 3 cut(s) 289, 317, 440
MfeI CAATTG 1 cut(s) 799
MflI RGATCY 1 cut(s) 701
MhlI GDGCHC 2 cut(s) 796, 936
MluCI AATT 9 cut(s) 151, 450, 526, 582, 646, 652, 762, 799, 1002
MlyI GAGTC 1 cut(s) 442
MmeI TCCRAC 1 cut(s) 46
MnlI CCTC 5 cut(s) 194, 499, 826, 871, 958
MslI CAYNNNNRTG 4 cut(s) 232, 267, 309, 926
MspA1I CMGCKG 1 cut(s) 821
MspI CCGG 2 cut(s) 125, 504
MspR9I CCNGG 3 cut(s) 221, 573, 960
MunI CAATTG 1 cut(s) 799
MvaI CCWGG 3 cut(s) 221, 573, 960
MwoI GCNNNNNNNGC 1 cut(s) 594
NcoI CCATGG 2 cut(s) 310, 577
NdeI CATATG 2 cut(s) 40, 172
NdeII GATC 1 cut(s) 701
NlaIII CATG 9 cut(s) 314, 350, 476, 488, 567, 581, 693, 749, 987
NlaIV GGNNCC 2 cut(s) 114, 502
NmeAIII GCCGAG 1 cut(s) 1047
NspI RCATGY 1 cut(s) 567
PfeI GAWTC 3 cut(s) 86, 146, 681
PflMI CCANNNNNTGG 1 cut(s) 578
PinAI ACCGGT 1 cut(s) 503
PleI GAGTC 1 cut(s) 441
PpsI GAGTC 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 796
Psp6I CCWGG 3 cut(s) 219, 571, 958
PspGI CCWGG 3 cut(s) 219, 571, 958
PspN4I GGNNCC 2 cut(s) 114, 502
PspPI GGNCC 2 cut(s) 569, 1051
PstI CTGCAG 2 cut(s) 389, 1041
PsuI RGATCY 1 cut(s) 701
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 3 cut(s) 8, 710, 1029
RsaNI GTAC 3 cut(s) 7, 709, 1028
RseI CAYNNNNRTG 4 cut(s) 232, 267, 309, 926
SacI GAGCTC 1 cut(s) 796
Sau3AI GATC 1 cut(s) 701
Sau96I GGNCC 2 cut(s) 569, 1051
ScaI AGTACT 1 cut(s) 1029
SchI GAGTC 1 cut(s) 442
ScrFI CCNGG 3 cut(s) 221, 573, 960
SduI GDGCHC 2 cut(s) 796, 936
SfaNI GCATC 2 cut(s) 118, 989
SfcI CTRYAG 2 cut(s) 385, 1037
SinI GGWCC 1 cut(s) 1051
SmiMI CAYNNNNRTG 4 cut(s) 232, 267, 309, 926
Sse9I AATT 9 cut(s) 151, 450, 526, 582, 646, 652, 762, 799, 1002
SsiI CCGC 1 cut(s) 198
SspI AATATT 1 cut(s) 779
SstI GAGCTC 1 cut(s) 796
StyD4I CCNGG 3 cut(s) 219, 571, 958
StyI CCWWGG 3 cut(s) 310, 577, 856
TaaI ACNGT 3 cut(s) 141, 625, 1018
TaqI TCGA 4 cut(s) 16, 213, 771, 950
TasI AATT 9 cut(s) 151, 450, 526, 582, 646, 652, 762, 799, 1002
TatI WGTACW 2 cut(s) 6, 1027
TfiI GAWTC 3 cut(s) 86, 146, 681
TscAI CASTG 2 cut(s) 146, 1023
TspDTI ATGAA 5 cut(s) 159, 206, 216, 473, 734
TspRI CASTG 2 cut(s) 146, 1023
Van91I CCANNNNNTGG 1 cut(s) 578
VpaK11BI GGWCC 1 cut(s) 1051
XapI RAATTY 1 cut(s) 1002
XceI RCATGY 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 1029
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.