RLG00000011536

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000579
Physical Location & Seq
Reverse (-)
10366609 .. 10369451
2843 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011536

Sequence Viewer

Length: 972 bp
ATGTTGGTCCTTGCTTCCTCTATTTTTCGCATTGGATTTGGCGATTTTAATGTGTTAGATTTTGAAGCAGCTGGAGATGGCAGAACTGATGATTCTCAACTTTCAGGGAATCTTGACTCACCCTCGAGTCTTCGTGAATGGAAAAACTCGGCATTTTGTTGGATATGCTTTTCAAATGTGAGGGATCTCACTCTCATGGGAACTGGACAGATAAATGGCCGTGGTTCAACTTGGTGGAACAAGGCTCTGCAGTTCGACAATTGTGACCAACTTACCCTAGATGGATTTACTTCTATTGACAGTCCAAGAGTTCATATCACAATGCACAAATGTACTGGTGTCCAAGTCTCAAACGTTCATATAAGTGCACCTGCAAGCAGCCCCAACACCGATGGCTTCTGTATCGTTGGGTCAACACAAGTAAATATAAGGGATTCTGTCATTGCAACTGGAGATGACTGCGTTGCAATTAAGGGAGGTTGCTCTTTTATAAACATAACCGGTGTGCAATGCGGACCAGGTCATGGTATCAGTGTGGGAAGCTTAGGCAAACAAGAAGGTCCGGATATAGTTGAAGAAGTACATGTTCAGAACTGTAAATTTACCAATTCTCAAAATGGTGCAAGAATCAAGACATTGCCGGGAGGGTATGGGCACGCTAGAGCGATATCTTTTGAGCAAATCACGCTTGAAAATGCCAAGAATCCCATTATAATTAAGCAAACGTACGTTGATAAAAATGGAGCAGTCAAGGTGAGTGATGTAACATTTCGTGGCTTTGAAGGAACATCAGCTTCCGCGCAAGCAATAACATTGGATTGCTCAGAGTTGGGCTGCACCAACATTGTAATGGATCAAATCGCCTTAAGCTCGGCCGTGCCAGGAAAGTCATTGCATTCTTATTGCAGAAATGCCCAAGGAAGATCAATCCTTACTTCACCTTATGTTCCTTGTCTAGAACAGTCAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

324

Amino Acids

34.48

Weight (kDa)

5.79

Isoelectric Point (pI)

36.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 44 - 305 3.3e-67 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 491, 713
AarI CACCTGC 1 cut(s) 379
Acc36I ACCTGC 1 cut(s) 379
AccB7I CCANNNNNTGG 1 cut(s) 524
AccII CGCG 1 cut(s) 800
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 2 cut(s) 513, 798
AclI AACGTT 1 cut(s) 354
AclWI GGATC 2 cut(s) 192, 861
AcoI YGGCCR 2 cut(s) 217, 873
AcsI RAATTY 1 cut(s) 599
AfaI GTAC 3 cut(s) 334, 582, 728
AfiI CCNNNNNNNGG 1 cut(s) 524
AflII CTTAAG 1 cut(s) 865
AflIII ACRYGT 1 cut(s) 583
AgeI ACCGGT 1 cut(s) 500
AgsI TTSAA 6 cut(s) 65, 174, 228, 575, 692, 782
AjnI CCWGG 2 cut(s) 517, 880
AluBI AGCT 4 cut(s) 71, 543, 794, 870
AluI AGCT 4 cut(s) 71, 543, 794, 870
Alw21I GWGCWC 1 cut(s) 370
Alw26I GTCTC 1 cut(s) 352
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 2 cut(s) 192, 861
Ama87I CYCGRG 1 cut(s) 124
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 2 cut(s) 217, 873
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 3 cut(s) 68, 378, 834
ApoI RAATTY 1 cut(s) 599
AsiGI ACCGGT 1 cut(s) 500
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 7, 515, 560
AsuC2I CCSGG 1 cut(s) 642
AsuHPI GGTGA 3 cut(s) 111, 766, 930
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 3 cut(s) 7, 515, 560
BaeGI GKGCMC 2 cut(s) 370, 657
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 3 cut(s) 80, 390, 821
BccI CCATC 3 cut(s) 71, 275, 386
BceAI ACGGC 2 cut(s) 204, 860
BciT130I CCWGG 2 cut(s) 519, 882
BcnI CCSGG 1 cut(s) 642
BcoDI GTCTC 1 cut(s) 352
BfaI CTAG 3 cut(s) 278, 660, 956
BfmI CTRYAG 1 cut(s) 248
BfrI CTTAAG 1 cut(s) 865
BfuAI ACCTGC 1 cut(s) 379
BisI GCNGC 3 cut(s) 69, 379, 835
BlsI GCNGC 3 cut(s) 70, 380, 836
Bme1390I CCNGG 3 cut(s) 519, 642, 882
Bme18I GGWCC 3 cut(s) 7, 515, 560
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 7, 515, 560
BmrFI CCNGG 3 cut(s) 519, 642, 882
BpiI GAAGAC 1 cut(s) 122
BpmI CTGGAG 2 cut(s) 93, 471
Bpu10I CCTNAGC 1 cut(s) 544
BpuMI CCSGG 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 220, 916
BsaWI WCCGGW 2 cut(s) 500, 562
Bsc4I CCNNNNNNNGG 1 cut(s) 524
Bse118I RCCGGY 1 cut(s) 500
Bse1I ACTGG 3 cut(s) 208, 340, 454
Bse3DI GCAATG 4 cut(s) 441, 515, 635, 890
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 2 cut(s) 519, 882
BseDI CCNNGG 2 cut(s) 220, 916
BseLI CCNNNNNNNGG 1 cut(s) 524
BseMI GCAATG 4 cut(s) 441, 515, 635, 890
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 3 cut(s) 208, 340, 454
BseSI GKGCMC 2 cut(s) 370, 657
BseX3I CGGCCG 1 cut(s) 873
BseXI GCAGC 3 cut(s) 80, 390, 821
BsgI GTGCAG 1 cut(s) 820
Bsh1236I CGCG 1 cut(s) 800
Bsh1285I CGRYCG 1 cut(s) 876
BshFI GGCC 2 cut(s) 219, 875
BshTI ACCGGT 1 cut(s) 500
BsiEI CGRYCG 1 cut(s) 876
BsiHKAI GWGCWC 1 cut(s) 370
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 3 cut(s) 501, 563, 641
BsiWI CGTACG 1 cut(s) 726
BslI CCNNNNNNNGG 1 cut(s) 524
BsmAI GTCTC 1 cut(s) 352
BsmI GAATGC 1 cut(s) 895
BsnI GGCC 2 cut(s) 219, 875
BsoBI CYCGRG 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 370, 657
Bsp13I TCCGGA 1 cut(s) 562
Bsp143I GATC 3 cut(s) 184, 853, 923
BspACI CCGC 2 cut(s) 513, 798
BspANI GGCC 2 cut(s) 219, 875
BspCNI CTCAG 1 cut(s) 836
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 1 cut(s) 800
BspMAI CTGCAG 1 cut(s) 252
BspMI ACCTGC 1 cut(s) 379
BspPI GGATC 2 cut(s) 192, 861
BspTI CTTAAG 1 cut(s) 865
BsrDI GCAATG 4 cut(s) 441, 515, 635, 890
BsrFI RCCGGY 1 cut(s) 500
BsrI ACTGG 3 cut(s) 208, 340, 454
BssAI RCCGGY 1 cut(s) 500
BssECI CCNNGG 2 cut(s) 220, 916
BssMI GATC 3 cut(s) 184, 853, 923
BssT1I CCWWGG 1 cut(s) 916
Bst2UI CCWGG 2 cut(s) 519, 882
Bst4CI ACNGT 3 cut(s) 302, 596, 963
BstAFI CTTAAG 1 cut(s) 865
BstC8I GCNNGC 3 cut(s) 376, 657, 804
BstDEI CTNAG 2 cut(s) 544, 823
BstDSI CCRYGG 1 cut(s) 220
BstFNI CGCG 1 cut(s) 800
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 3 cut(s) 187, 856, 926
BstMAI GTCTC 1 cut(s) 352
BstMBI GATC 3 cut(s) 184, 853, 923
BstMCI CGRYCG 1 cut(s) 876
BstMWI GCNNNNNNNGC 1 cut(s) 685
BstNI CCWGG 2 cut(s) 519, 882
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 3 cut(s) 517, 640, 880
BstSFI CTRYAG 1 cut(s) 248
BstSLI GKGCMC 2 cut(s) 370, 657
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 3 cut(s) 80, 390, 821
BstV2I GAAGAC 1 cut(s) 122
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BstZI CGGCCG 1 cut(s) 873
BsuRI GGCC 2 cut(s) 219, 875
BtgI CCRYGG 1 cut(s) 220
BtsIMutI CAGTG 1 cut(s) 538
BveI ACCTGC 1 cut(s) 379
Cac8I GCNNGC 3 cut(s) 376, 657, 804
CfoI GCGC 1 cut(s) 802
Cfr10I RCCGGY 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 7, 515, 560
CsiI ACCWGGT 1 cut(s) 517
Csp6I GTAC 3 cut(s) 333, 581, 727
CspAI ACCGGT 1 cut(s) 500
CviAII CATG 3 cut(s) 196, 524, 584
CviQI GTAC 3 cut(s) 333, 581, 727
DdeI CTNAG 2 cut(s) 544, 823
DpnI GATC 3 cut(s) 186, 855, 925
DpnII GATC 3 cut(s) 184, 853, 923
EaeI YGGCCR 2 cut(s) 217, 873
EagI CGGCCG 1 cut(s) 873
EclXI CGGCCG 1 cut(s) 873
Eco130I CCWWGG 1 cut(s) 916
Eco32I GATATC 1 cut(s) 669
Eco47I GGWCC 3 cut(s) 7, 515, 560
Eco52I CGGCCG 1 cut(s) 873
Eco88I CYCGRG 1 cut(s) 124
EcoRII CCWGG 2 cut(s) 517, 880
EcoRV GATATC 1 cut(s) 669
EcoT14I CCWWGG 1 cut(s) 916
ErhI CCWWGG 1 cut(s) 916
FaeI CATG 3 cut(s) 199, 527, 587
FatI CATG 3 cut(s) 195, 523, 583
Fnu4HI GCNGC 3 cut(s) 69, 379, 835
Fsp4HI GCNGC 3 cut(s) 69, 379, 835
FspBI CTAG 3 cut(s) 278, 660, 956
GlaI GCGC 1 cut(s) 801
GluI GCNGC 3 cut(s) 69, 379, 835
GsuI CTGGAG 2 cut(s) 93, 471
HaeIII GGCC 2 cut(s) 219, 875
HapII CCGG 3 cut(s) 501, 563, 641
HhaI GCGC 1 cut(s) 802
Hin1II CATG 3 cut(s) 199, 527, 587
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HincII GTYRAC 1 cut(s) 414
HindII GTYRAC 1 cut(s) 414
HindIII AAGCTT 1 cut(s) 541
HinfI GANTC 7 cut(s) 92, 109, 116, 127, 434, 627, 703
HpaII CCGG 3 cut(s) 501, 563, 641
HphI GGTGA 3 cut(s) 111, 766, 930
Hpy166II GTNNAC 2 cut(s) 368, 414
Hpy188I TCNGA 2 cut(s) 591, 826
Hpy188III TCNNGA 5 cut(s) 113, 134, 563, 631, 956
Hpy8I GTNNAC 2 cut(s) 368, 414
HpyAV CCTTC 2 cut(s) 551, 776
HpyCH4III ACNGT 3 cut(s) 302, 596, 963
HpyCH4IV ACGT 3 cut(s) 354, 725, 729
HpyF10VI GCNNNNNNNGC 1 cut(s) 685
HpyF3I CTNAG 2 cut(s) 544, 823
HpySE526I ACGT 3 cut(s) 354, 725, 729
Hsp92II CATG 3 cut(s) 199, 527, 587
HspAI GCGC 1 cut(s) 800
Kpn2I TCCGGA 1 cut(s) 562
Kzo9I GATC 3 cut(s) 184, 853, 923
LmnI GCTCC 1 cut(s) 743
Lsp1109I GCAGC 3 cut(s) 80, 390, 821
MabI ACCWGGT 1 cut(s) 517
MaeI CTAG 3 cut(s) 278, 660, 956
MaeII ACGT 3 cut(s) 354, 725, 729
MaeIII GTNAC 2 cut(s) 263, 763
MalI GATC 3 cut(s) 186, 855, 925
MboI GATC 3 cut(s) 184, 853, 923
MboII GAAGA 3 cut(s) 122, 587, 933
MfeI CAATTG 1 cut(s) 259
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 370, 657
MluCI AATT 5 cut(s) 259, 468, 599, 607, 714
MlyI GAGTC 2 cut(s) 110, 136
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 5 cut(s) 28, 133, 174, 470, 638
MroI TCCGGA 1 cut(s) 562
MseI TTAA 4 cut(s) 48, 471, 717, 866
MslI CAYNNNNRTG 3 cut(s) 194, 363, 848
MspA1I CMGCKG 1 cut(s) 71
MspCI CTTAAG 1 cut(s) 865
MspI CCGG 3 cut(s) 501, 563, 641
MspR9I CCNGG 3 cut(s) 519, 642, 882
MunI CAATTG 1 cut(s) 259
Mva1269I GAATGC 1 cut(s) 895
MvaI CCWGG 2 cut(s) 519, 882
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 1 cut(s) 685
NciI CCSGG 1 cut(s) 642
NdeII GATC 3 cut(s) 184, 853, 923
NlaIII CATG 3 cut(s) 199, 527, 587
NmeAIII GCCGAG 2 cut(s) 128, 851
NmuCI GTSAC 1 cut(s) 263
NspI RCATGY 1 cut(s) 587
PaeR7I CTCGAG 1 cut(s) 124
PaqCI CACCTGC 1 cut(s) 379
PciI ACATGT 1 cut(s) 583
PctI GAATGC 1 cut(s) 895
PfeI GAWTC 5 cut(s) 92, 109, 434, 627, 703
Pfl23II CGTACG 1 cut(s) 726
PflFI GACNNNGTC 1 cut(s) 519
PflMI CCANNNNNTGG 1 cut(s) 524
PinAI ACCGGT 1 cut(s) 500
PkrI GCNGC 3 cut(s) 70, 380, 836
PleI GAGTC 2 cut(s) 110, 135
PpsI GAGTC 2 cut(s) 110, 135
PscI ACATGT 1 cut(s) 583
PsiI TTATAA 2 cut(s) 491, 713
Psp1406I AACGTT 1 cut(s) 354
Psp6I CCWGG 2 cut(s) 517, 880
PspGI CCWGG 2 cut(s) 517, 880
PspLI CGTACG 1 cut(s) 726
PspPI GGNCC 3 cut(s) 7, 515, 560
PspXI VCTCGAGB 1 cut(s) 124
PstI CTGCAG 1 cut(s) 252
PsuI RGATCY 1 cut(s) 184
PsyI GACNNNGTC 1 cut(s) 519
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 3 cut(s) 334, 582, 728
RsaNI GTAC 3 cut(s) 333, 581, 727
RseI CAYNNNNRTG 3 cut(s) 194, 363, 848
SaqAI TTAA 4 cut(s) 48, 471, 717, 866
SatI GCNGC 3 cut(s) 69, 379, 835
Sau3AI GATC 3 cut(s) 184, 853, 923
Sau96I GGNCC 3 cut(s) 7, 515, 560
SchI GAGTC 2 cut(s) 110, 136
ScrFI CCNGG 3 cut(s) 519, 642, 882
SduI GDGCHC 2 cut(s) 370, 657
SexAI ACCWGGT 1 cut(s) 517
SfcI CTRYAG 1 cut(s) 248
Sfr274I CTCGAG 1 cut(s) 124
SinI GGWCC 3 cut(s) 7, 515, 560
SlaI CTCGAG 1 cut(s) 124
SmiMI CAYNNNNRTG 3 cut(s) 194, 363, 848
SmlI CTYRAG 2 cut(s) 124, 865
SmoI CTYRAG 2 cut(s) 124, 865
Sse9I AATT 5 cut(s) 259, 468, 599, 607, 714
SsiI CCGC 2 cut(s) 513, 798
SspMI CTAG 3 cut(s) 278, 660, 956
StyD4I CCNGG 3 cut(s) 517, 640, 880
StyI CCWWGG 1 cut(s) 916
TaaI ACNGT 3 cut(s) 302, 596, 963
TaiI ACGT 3 cut(s) 357, 728, 732
TaqI TCGA 2 cut(s) 125, 255
TasI AATT 5 cut(s) 259, 468, 599, 607, 714
TatI WGTACW 2 cut(s) 332, 580
TfiI GAWTC 5 cut(s) 92, 109, 434, 627, 703
Tru1I TTAA 4 cut(s) 48, 471, 717, 866
Tru9I TTAA 4 cut(s) 48, 471, 717, 866
TscAI CASTG 1 cut(s) 538
TseFI GTSAC 1 cut(s) 263
TseI GCWGC 3 cut(s) 68, 378, 834
Tsp45I GTSAC 1 cut(s) 263
TspDTI ATGAA 2 cut(s) 302, 347
TspRI CASTG 1 cut(s) 538
Tth111I GACNNNGTC 1 cut(s) 519
Van91I CCANNNNNTGG 1 cut(s) 524
Vha464I CTTAAG 1 cut(s) 865
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 3 cut(s) 7, 515, 560
XapI RAATTY 1 cut(s) 599
XbaI TCTAGA 1 cut(s) 955
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 847
XhoI CTCGAG 1 cut(s) 124
XspI CTAG 3 cut(s) 278, 660, 956
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.