Prupe.1G247300_v2.0.a1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000579
Physical Location & Seq
Reverse (-)
25998579 .. 26000729
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G247300.2

Sequence Viewer

Length: 945 bp
ATGTTTGAGAAGATCACACTCGATAGAGCTCAAAATTCCATCATCATTGACCAGTTTTATTGTAATCGTGATCACAAATGCAAAACTCAGGCCTCGGCTGTGTCGGTGGATGATGTGAAGTACATTGATTTTGAAGGAACTTCTGCGAGTGAGGAGGCGATCAAATTAGATTGTGACCAGAATTCAAGTTGCCACAACATTATTATGGACCGCATCAAAGTTACCTCTGATGTTCCTGGTAAGAAGATTTATGCCTCTTGCAATAATGCTATTGGAACTTCCATTGGCACTATTGTGCCCAATGTACCTTGCCTCAAGGCAGGGGCAGAACCAACTACTTATTCGCCAGCTACGCCTCCACAAGCTCTGCCTTTGCCACCTACGCCTCTGCCATCTCTGCCTTTGCCTGTTACACCTCCGCCATCTCTGTCTATGCCGTCAATAGATCAGCCATCTCTGCCTATGCTGGCTGAGCCTTCATTAGCTCTAATTTTGCCAGCTACACCTCCGCCAGCTCCGCACTTGCCAGATACACCTCCGGCATCTCCGCCTGTGCTGGCTACACCTCCGCCATCTCCACCTATGCCGGCTACGCCTCCTCTAGCTCTAATTTTGCCAGCTACACCTCCGCCATTGACGGCTACGTCTCCGCCATCTTTGCCCATGCCAGCTACGCCTCTACCGATTCTGCCTCCCCTAGCTCCACCTTTGCCAAATAAGCCTCGACCAACTCTGCCTTTGCCAACTACGCCTCCGCTAGCTCGGCCTTTGCGAAATAGGCCTCGGCCTCTACCTTTGCCAACTATGCCTTCCCCGGCTCCACCTCTGCCATTTACACCCTTAGCTTCGCTAGTACCAACTCTGCCTCCCCCAGCTCTGCCAGCAACACCACAAATGTCCTTTAAAGGGTACTCAGTAAGGCCTAGGGCACCGGTGTGGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

315

Amino Acids

33.12

Weight (kDa)

8.52

Isoelectric Point (pI)

79.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 928
AciI CCGC 9 cut(s) 211, 419, 509, 518, 548, 569, 629, 650, 755
AcsI RAATTY 2 cut(s) 34, 181
AfaI GTAC 5 cut(s) 122, 306, 855, 911, 941
AfiI CCNNNNNNNGG 1 cut(s) 906
AgeI ACCGGT 1 cut(s) 931
AgsI TTSAA 2 cut(s) 134, 186
AjnI CCWGG 1 cut(s) 235
AjuI GAANNNNNNNTTGG 4 cut(s) 325, 357, 793, 825
AleI CACNNNNGTG 2 cut(s) 293, 934
Alw21I GWGCWC 1 cut(s) 31
Alw26I GTCTC 1 cut(s) 651
AoxI GGCC 5 cut(s) 90, 764, 779, 785, 920
ApoI RAATTY 2 cut(s) 34, 181
AsiGI ACCGGT 1 cut(s) 931
AspA2I CCTAGG 1 cut(s) 923
AspS9I GGNCC 1 cut(s) 208
AsuC2I CCSGG 1 cut(s) 815
AsuNHI GCTAGC 1 cut(s) 757
AvaII GGWCC 1 cut(s) 208
AvrII CCTAGG 1 cut(s) 923
BaeGI GKGCMC 2 cut(s) 300, 931
BanI GGYRCC 1 cut(s) 928
BanII GRGCYC 1 cut(s) 31
Bbv12I GWGCWC 1 cut(s) 31
BccI CCATC 6 cut(s) 47, 400, 430, 460, 580, 661
BceAI ACGGC 2 cut(s) 421, 654
BciT130I CCWGG 1 cut(s) 237
BclI TGATCA 1 cut(s) 70
BcnI CCSGG 1 cut(s) 815
BcoDI GTCTC 1 cut(s) 651
BfaI CTAG 6 cut(s) 602, 698, 758, 851, 924, 943
BlnI CCTAGG 1 cut(s) 923
BlpI GCTNAGC 1 cut(s) 471
Bme1390I CCNGG 2 cut(s) 237, 815
Bme18I GGWCC 1 cut(s) 208
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 2 cut(s) 819, 930
BmrFI CCNGG 2 cut(s) 237, 815
BmsI GCATC 2 cut(s) 222, 551
BmtI GCTAGC 1 cut(s) 761
Bpu10I CCTNAGC 1 cut(s) 841
Bpu1102I GCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 815
BsaJI CCNNGG 4 cut(s) 93, 782, 813, 923
BsaWI WCCGGW 1 cut(s) 931
BsaXI ACNNNNNCTCC 4 cut(s) 736, 766, 850, 880
Bsc4I CCNNNNNNNGG 1 cut(s) 906
Bse118I RCCGGY 2 cut(s) 586, 931
Bse1I ACTGG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 4 cut(s) 93, 782, 813, 923
BseGI GGATG 1 cut(s) 115
BseLI CCNNNNNNNGG 1 cut(s) 906
BseMII CTCAG 3 cut(s) 101, 462, 927
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 2 cut(s) 167, 588
BseSI GKGCMC 2 cut(s) 300, 931
BseYI CCCAGC 1 cut(s) 871
BshFI GGCC 5 cut(s) 92, 766, 781, 787, 922
BshNI GGYRCC 1 cut(s) 928
BshTI ACCGGT 1 cut(s) 931
BsiHKAI GWGCWC 1 cut(s) 31
BsiSI CCGG 4 cut(s) 539, 587, 815, 932
BslI CCNNNNNNNGG 1 cut(s) 906
BsmAI GTCTC 1 cut(s) 651
BsmBI CGTCTC 1 cut(s) 651
BsnI GGCC 5 cut(s) 92, 766, 781, 787, 922
Bsp1286I GDGCHC 3 cut(s) 31, 300, 931
Bsp143I GATC 4 cut(s) 12, 70, 159, 445
Bsp1720I GCTNAGC 1 cut(s) 471
BspACI CCGC 9 cut(s) 211, 419, 509, 518, 548, 569, 629, 650, 755
BspANI GGCC 5 cut(s) 92, 766, 781, 787, 922
BspCNI CTCAG 3 cut(s) 100, 463, 926
BspLI GGNNCC 2 cut(s) 819, 930
BspOI GCTAGC 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 928
BsrFI RCCGGY 2 cut(s) 586, 931
BsrI ACTGG 1 cut(s) 52
BssAI RCCGGY 2 cut(s) 586, 931
BssECI CCNNGG 4 cut(s) 93, 782, 813, 923
BssMI GATC 4 cut(s) 12, 70, 159, 445
BssT1I CCWWGG 1 cut(s) 923
Bst2UI CCWGG 1 cut(s) 237
BstDEI CTNAG 4 cut(s) 87, 471, 841, 913
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 4 cut(s) 15, 73, 162, 448
BstMAI GTCTC 1 cut(s) 651
BstMBI GATC 4 cut(s) 12, 70, 159, 445
BstNI CCWGG 1 cut(s) 237
BstSCI CCNGG 2 cut(s) 235, 813
BstSLI GKGCMC 2 cut(s) 300, 931
BsuRI GGCC 5 cut(s) 92, 766, 781, 787, 922
BtsCI GGATG 1 cut(s) 115
Cfr10I RCCGGY 2 cut(s) 586, 931
Cfr13I GGNCC 1 cut(s) 208
Csp6I GTAC 5 cut(s) 121, 305, 854, 910, 940
CspAI ACCGGT 1 cut(s) 931
CviAII CATG 1 cut(s) 664
CviQI GTAC 5 cut(s) 121, 305, 854, 910, 940
DdeI CTNAG 4 cut(s) 87, 471, 841, 913
DpnI GATC 4 cut(s) 14, 72, 161, 447
DpnII GATC 4 cut(s) 12, 70, 159, 445
DraI TTTAAA 1 cut(s) 904
DrdI GACNNNNNNGTC 1 cut(s) 643
DseDI GACNNNNNNGTC 1 cut(s) 643
EciI GGCGGA 6 cut(s) 408, 498, 537, 558, 618, 639
Ecl136II GAGCTC 1 cut(s) 29
Eco130I CCWWGG 1 cut(s) 923
Eco147I AGGCCT 3 cut(s) 92, 781, 922
Eco24I GRGCYC 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 29
EcoICRI GAGCTC 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 923
EcoT38I GRGCYC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 923
Esp3I CGTCTC 1 cut(s) 651
FaeI CATG 1 cut(s) 667
FaiI YATR 7 cut(s) 206, 252, 434, 464, 584, 665, 806
FatI CATG 1 cut(s) 663
FbaI TGATCA 1 cut(s) 70
FokI GGATG 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 31
FspBI CTAG 6 cut(s) 602, 698, 758, 851, 924, 943
GsaI CCCAGC 1 cut(s) 875
HaeIII GGCC 5 cut(s) 92, 766, 781, 787, 922
HapII CCGG 4 cut(s) 539, 587, 815, 932
Hin1II CATG 1 cut(s) 667
HinfI GANTC 1 cut(s) 685
HpaII CCGG 4 cut(s) 539, 587, 815, 932
Hpy188I TCNGA 1 cut(s) 229
Hpy188III TCNNGA 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 128, 486, 819
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 2 cut(s) 81, 261
HpyF3I CTNAG 4 cut(s) 87, 471, 841, 913
HpySE526I ACGT 1 cut(s) 644
Hsp92II CATG 1 cut(s) 667
KroI GCCGGC 1 cut(s) 586
KroNI GCCGGC 1 cut(s) 588
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 4 cut(s) 12, 70, 159, 445
LmnI GCTCC 3 cut(s) 520, 706, 823
LweI GCATC 2 cut(s) 222, 551
MaeI CTAG 6 cut(s) 602, 698, 758, 851, 924, 943
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 3 cut(s) 173, 220, 409
MalI GATC 4 cut(s) 14, 72, 161, 447
MboI GATC 4 cut(s) 12, 70, 159, 445
MboII GAAGA 2 cut(s) 22, 256
MhlI GDGCHC 3 cut(s) 31, 300, 931
MluCI AATT 5 cut(s) 34, 164, 181, 489, 609
MroNI GCCGGC 1 cut(s) 586
MseI TTAA 1 cut(s) 903
MslI CAYNNNNRTG 3 cut(s) 203, 293, 934
MspI CCGG 4 cut(s) 539, 587, 815, 932
MspR9I CCNGG 2 cut(s) 237, 815
MvaI CCWGG 1 cut(s) 237
NaeI GCCGGC 1 cut(s) 588
NciI CCSGG 1 cut(s) 815
NdeII GATC 4 cut(s) 12, 70, 159, 445
NgoMIV GCCGGC 1 cut(s) 586
NheI GCTAGC 1 cut(s) 757
NlaIII CATG 1 cut(s) 667
NlaIV GGNNCC 2 cut(s) 819, 930
NmeAIII GCCGAG 3 cut(s) 74, 742, 763
NmuCI GTSAC 1 cut(s) 173
OliI CACNNNNGTG 2 cut(s) 293, 934
PceI AGGCCT 3 cut(s) 92, 781, 922
PcsI WCGNNNNNNNCGW 2 cut(s) 680, 769
PdiI GCCGGC 1 cut(s) 588
PfeI GAWTC 1 cut(s) 685
PinAI ACCGGT 1 cut(s) 931
Psp124BI GAGCTC 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 871
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 2 cut(s) 819, 930
PspPI GGNCC 1 cut(s) 208
RsaI GTAC 5 cut(s) 122, 306, 855, 911, 941
RsaNI GTAC 5 cut(s) 121, 305, 854, 910, 940
RseI CAYNNNNRTG 3 cut(s) 203, 293, 934
SacI GAGCTC 1 cut(s) 31
SaqAI TTAA 1 cut(s) 903
Sau3AI GATC 4 cut(s) 12, 70, 159, 445
Sau96I GGNCC 1 cut(s) 208
ScrFI CCNGG 2 cut(s) 237, 815
SduI GDGCHC 3 cut(s) 31, 300, 931
SfaNI GCATC 2 cut(s) 222, 551
SgrAI CRCCGGYG 1 cut(s) 931
SinI GGWCC 1 cut(s) 208
SmiMI CAYNNNNRTG 3 cut(s) 203, 293, 934
SmlI CTYRAG 1 cut(s) 314
SmoI CTYRAG 1 cut(s) 314
Sse9I AATT 5 cut(s) 34, 164, 181, 489, 609
SseBI AGGCCT 3 cut(s) 92, 781, 922
SsiI CCGC 9 cut(s) 211, 419, 509, 518, 548, 569, 629, 650, 755
SspMI CTAG 6 cut(s) 602, 698, 758, 851, 924, 943
SstI GAGCTC 1 cut(s) 31
StuI AGGCCT 3 cut(s) 92, 781, 922
StyD4I CCNGG 2 cut(s) 235, 813
StyI CCWWGG 1 cut(s) 923
TaiI ACGT 1 cut(s) 647
TaqI TCGA 2 cut(s) 21, 724
TasI AATT 5 cut(s) 34, 164, 181, 489, 609
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 1 cut(s) 903
Tru9I TTAA 1 cut(s) 903
TseFI GTSAC 1 cut(s) 173
Tsp45I GTSAC 1 cut(s) 173
TspDTI ATGAA 1 cut(s) 468
VpaK11BI GGWCC 1 cut(s) 208
XapI RAATTY 2 cut(s) 34, 181
XmaJI CCTAGG 1 cut(s) 923
XspI CTAG 6 cut(s) 602, 698, 758, 851, 924, 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.