RLG00000023386

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000579
Physical Location & Seq
Forward (+)
22937519 .. 22940535
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023386

Sequence Viewer

Length: 1182 bp
ATGAGAAAACTACTTGCAAGCTTTTTGATAGTATGCATTTTTACTACATCGTCAAATCTTAATGTTGGGTATTGCCAGGAAACTTTTAATGTGCTTGACTACGGTGCTGTTGGAGATGGCCAAACCGATGATTCTGGAATTTTGGGGAAAATTGTGGCACCCAAAACTCCTCAAGAATGGAAAGAGTGTGAAAAAGAATCCTGGCTGCTCTTTTCAGAGGTGAACAACCTGATCATTAATGGAAATGAAACAGGAGTAATCAATGGGAATGGTTTACCTTGGTACACAGAGGTGAATAAGTCGGAAGTAGAGAAGACAAAATGCGATAGACCAAAGGCTCTACGCTTCTTCAATTGTAATGATCTTCAGTTAAGTGGACTCACACACGTTGATAGTCCAAAAGGGCATATTAGCATACTAAACTGCACCAATGTTATAGTATCCAATCTTCATATCATTGCACCAGATGATAGTGAAAACACAGATGGCATTGACATCAATATGTCAAGACATGTCAATATTCATGACTGCATAATTGGAACAGGAGACGATTGCATTGCTATTAAAGGTGGCTCGGTCCTTGTCAATGTCGCCAACATTGCTTGCGGACCGGGTCATGGAATTAGTGTGGGAAGCATAGGTAAAGAAGGAGCTTATGAGACAGTCGCAGAGGTTTATGTACGAAATTGTACCTTCAATGGTACGATGAATGGAGCTAGAATCAAGACATGGCCGGGTGGTTCTGGATATGCCAAGAATATCTTGTATGAGAAAATCACTCTAATAGGAGCTCAAAATCCTATTATTATTGATCAAAACTACTGCAATGGTGAACACGACTGCGGTGATATAACGTCGAATTCAGCAGTGGAGGTGAGTAATGTGACATTCAATGACTTTCGAGGAACTCTGGCGGATGATCATGCGATTACATTTGATTGCTCCAAGACTGCAGATGGCACTCTAGCTTGTCTCAACATTAATATGAACCAAATCAACATCACTTCCATAGATCCCGAAAAGGAAATCTCTGTCTTATGCAAGAATGTAAATGGAACTACTGAAGATACCAGTCCCAGTGTTCATTGCCTACAAGCTAGAAATTCAACCATTGATGCAGATAGTCCTGATGCATATAGTCCTGATCCCTACGCCTTTTCTTTTTACAACCATGTATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

394

Amino Acids

42.5

Weight (kDa)

4.69

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 351 3.1e-73 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AciI CCGC 3 cut(s) 606, 843, 914
AclWI GGATC 2 cut(s) 1007, 1139
AcoI YGGCCR 2 cut(s) 118, 731
AcsI RAATTY 3 cut(s) 138, 859, 1102
AcuI CTGAAG 2 cut(s) 350, 1083
AfaI GTAC 4 cut(s) 284, 681, 691, 703
AfiI CCNNNNNNNGG 1 cut(s) 617
AflIII ACRYGT 2 cut(s) 385, 511
AgsI TTSAA 4 cut(s) 352, 697, 892, 1107
AjnI CCWGG 2 cut(s) 75, 200
AleI CACNNNNGTG 1 cut(s) 290
AluBI AGCT 6 cut(s) 21, 653, 716, 791, 968, 1097
AluI AGCT 6 cut(s) 21, 653, 716, 791, 968, 1097
Alw21I GWGCWC 1 cut(s) 793
Alw26I GTCTC 3 cut(s) 540, 653, 977
AlwI GGATC 2 cut(s) 1007, 1139
AoxI GGCC 2 cut(s) 118, 731
ApeKI GCWGC 1 cut(s) 205
ApoI RAATTY 3 cut(s) 138, 859, 1102
AseI ATTAAT 2 cut(s) 237, 981
AspS9I GGNCC 2 cut(s) 577, 608
AsuC2I CCSGG 2 cut(s) 612, 735
AsuHPI GGTGA 5 cut(s) 232, 304, 842, 857, 886
AvaII GGWCC 2 cut(s) 577, 608
BalI TGGCCA 1 cut(s) 120
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 793
BarI GAAGNNNNNNTAC 2 cut(s) 432, 464
BbsI GAAGAC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 793
BbvI GCAGC 1 cut(s) 192
BccI CCATC 3 cut(s) 110, 479, 950
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 2 cut(s) 77, 202
BciVI GTATCC 1 cut(s) 451
BclI TGATCA 3 cut(s) 231, 811, 919
BcnI CCSGG 2 cut(s) 612, 735
BcoDI GTCTC 3 cut(s) 540, 653, 977
BfaI CTAG 3 cut(s) 717, 965, 1098
BfmI CTRYAG 1 cut(s) 951
BfuI GTATCC 1 cut(s) 451
BisI GCNGC 1 cut(s) 206
BlsI GCNGC 1 cut(s) 207
Bme1390I CCNGG 4 cut(s) 77, 202, 612, 735
Bme18I GGWCC 2 cut(s) 577, 608
BmgT120I GGNCC 2 cut(s) 577, 608
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 4 cut(s) 77, 202, 612, 735
BmrI ACTGGG 1 cut(s) 1071
BmsI GCATC 2 cut(s) 1105, 1120
BmuI ACTGGG 1 cut(s) 1071
BpiI GAAGAC 1 cut(s) 320
BpuEI CTTGAG 1 cut(s) 156
BpuMI CCSGG 2 cut(s) 612, 735
BsaJI CCNNGG 1 cut(s) 278
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 1071, 1077
Bse3DI GCAATG 5 cut(s) 456, 555, 597, 832, 1084
BseBI CCWGG 2 cut(s) 77, 202
BseDI CCNNGG 1 cut(s) 278
BseGI GGATG 1 cut(s) 922
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMI GCAATG 5 cut(s) 456, 555, 597, 832, 1084
BseNI ACTGG 2 cut(s) 1071, 1077
BseRI GAGGAG 1 cut(s) 159
BseXI GCAGC 1 cut(s) 192
BsgI GTGCAG 1 cut(s) 409
BshFI GGCC 2 cut(s) 120, 733
BshNI GGYRCC 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 793
BsiSI CCGG 2 cut(s) 611, 734
BslFI GGGAC 1 cut(s) 1059
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 3 cut(s) 540, 653, 977
BsmBI CGTCTC 1 cut(s) 540
BsmFI GGGAC 1 cut(s) 1059
BsnI GGCC 2 cut(s) 120, 733
Bsp1286I GDGCHC 1 cut(s) 793
Bsp143I GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
BspACI CCGC 3 cut(s) 606, 843, 914
BspANI GGCC 2 cut(s) 120, 733
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 1 cut(s) 159
BspMAI CTGCAG 1 cut(s) 955
BspPI GGATC 2 cut(s) 1007, 1139
BspT107I GGYRCC 1 cut(s) 157
BsrDI GCAATG 5 cut(s) 456, 555, 597, 832, 1084
BsrI ACTGG 2 cut(s) 1071, 1077
BssECI CCNNGG 1 cut(s) 278
BssMI GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
BssT1I CCWWGG 1 cut(s) 278
Bst2UI CCWGG 2 cut(s) 77, 202
Bst4CI ACNGT 2 cut(s) 104, 664
BstC8I GCNNGC 2 cut(s) 19, 604
BstF5I GGATG 1 cut(s) 922
BstKTI GATC 6 cut(s) 234, 364, 814, 922, 1015, 1147
BstMAI GTCTC 3 cut(s) 540, 653, 977
BstMBI GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
BstMWI GCNNNNNNNGC 1 cut(s) 599
BstNI CCWGG 2 cut(s) 77, 202
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 4 cut(s) 75, 200, 610, 733
BstSFI CTRYAG 1 cut(s) 951
BstV1I GCAGC 1 cut(s) 192
BstV2I GAAGAC 1 cut(s) 320
BstX2I RGATCY 1 cut(s) 1012
BstYI RGATCY 1 cut(s) 1012
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 2 cut(s) 120, 733
BtsCI GGATG 1 cut(s) 922
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 2 cut(s) 873, 1084
Cac8I GCNNGC 2 cut(s) 19, 604
CciI TCATGA 1 cut(s) 523
Cfr13I GGNCC 2 cut(s) 577, 608
CpoI CGGWCCG 1 cut(s) 608
Csp6I GTAC 4 cut(s) 283, 680, 690, 702
CspI CGGWCCG 1 cut(s) 608
CviAII CATG 6 cut(s) 512, 524, 617, 729, 923, 1172
CviQI GTAC 4 cut(s) 283, 680, 690, 702
DpnI GATC 6 cut(s) 233, 363, 813, 921, 1014, 1146
DpnII GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
EaeI YGGCCR 2 cut(s) 118, 731
EciI GGCGGA 1 cut(s) 929
Ecl136II GAGCTC 1 cut(s) 791
Eco130I CCWWGG 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 793
Eco47I GGWCC 2 cut(s) 577, 608
Eco53kI GAGCTC 1 cut(s) 791
Eco57I CTGAAG 2 cut(s) 350, 1083
EcoICRI GAGCTC 1 cut(s) 791
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 2 cut(s) 75, 200
EcoT14I CCWWGG 1 cut(s) 278
EcoT22I ATGCAT 2 cut(s) 38, 1135
EcoT38I GRGCYC 1 cut(s) 793
ErhI CCWWGG 1 cut(s) 278
Esp3I CGTCTC 1 cut(s) 540
FaeI CATG 6 cut(s) 515, 527, 620, 732, 926, 1175
FalI AAGNNNNNCTT 2 cut(s) 746, 778
FaqI GGGAC 1 cut(s) 1059
FatI CATG 6 cut(s) 511, 523, 616, 728, 922, 1171
FbaI TGATCA 3 cut(s) 231, 811, 919
Fnu4HI GCNGC 1 cut(s) 206
FokI GGATG 1 cut(s) 929
FriOI GRGCYC 1 cut(s) 793
Fsp4HI GCNGC 1 cut(s) 206
FspBI CTAG 3 cut(s) 717, 965, 1098
GluI GCNGC 1 cut(s) 206
HaeIII GGCC 2 cut(s) 120, 733
HapII CCGG 2 cut(s) 611, 734
Hin1II CATG 6 cut(s) 515, 527, 620, 732, 926, 1175
HindIII AAGCTT 1 cut(s) 19
HinfI GANTC 4 cut(s) 131, 197, 378, 720
HpaII CCGG 2 cut(s) 611, 734
HphI GGTGA 5 cut(s) 232, 304, 842, 857, 886
Hpy166II GTNNAC 5 cut(s) 223, 275, 285, 377, 833
Hpy188I TCNGA 2 cut(s) 217, 304
Hpy188III TCNNGA 9 cut(s) 135, 173, 507, 524, 724, 744, 1016, 1127, 1142
Hpy8I GTNNAC 5 cut(s) 223, 275, 285, 377, 833
Hpy99I CGWCG 1 cut(s) 859
HpyAV CCTTC 2 cut(s) 641, 703
HpyCH4III ACNGT 2 cut(s) 104, 664
HpyCH4IV ACGT 2 cut(s) 387, 854
HpyF10VI GCNNNNNNNGC 1 cut(s) 599
HpySE526I ACGT 2 cut(s) 387, 854
Hsp92II CATG 6 cut(s) 515, 527, 620, 732, 926, 1175
Ksp22I TGATCA 3 cut(s) 231, 811, 919
Kzo9I GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
LmnI GCTCC 4 cut(s) 650, 713, 788, 947
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 2 cut(s) 1105, 1120
MaeI CTAG 3 cut(s) 717, 965, 1098
MaeII ACGT 2 cut(s) 387, 854
MaeIII GTNAC 1 cut(s) 883
MalI GATC 6 cut(s) 233, 363, 813, 921, 1014, 1146
MboI GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
MboII GAAGA 5 cut(s) 325, 340, 356, 440, 1076
MfeI CAATTG 1 cut(s) 352
MflI RGATCY 1 cut(s) 1012
MhlI GDGCHC 1 cut(s) 793
MlsI TGGCCA 1 cut(s) 120
MluCI AATT 8 cut(s) 138, 150, 352, 534, 621, 685, 859, 1102
MluNI TGGCCA 1 cut(s) 120
MlyI GAGTC 1 cut(s) 372
MmeI TCCRAC 2 cut(s) 91, 282
MnlI CCTC 6 cut(s) 180, 211, 283, 664, 865, 896
Mox20I TGGCCA 1 cut(s) 120
Mph1103I ATGCAT 2 cut(s) 38, 1135
MscI TGGCCA 1 cut(s) 120
MseI TTAA 6 cut(s) 60, 87, 237, 371, 564, 981
MslI CAYNNNNRTG 3 cut(s) 290, 500, 983
Msp20I TGGCCA 1 cut(s) 120
MspI CCGG 2 cut(s) 611, 734
MspR9I CCNGG 4 cut(s) 77, 202, 612, 735
MunI CAATTG 1 cut(s) 352
MvaI CCWGG 2 cut(s) 77, 202
MwoI GCNNNNNNNGC 1 cut(s) 599
NciI CCSGG 2 cut(s) 612, 735
NdeII GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
NlaIII CATG 6 cut(s) 515, 527, 620, 732, 926, 1175
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 883
NsiI ATGCAT 2 cut(s) 38, 1135
NspI RCATGY 1 cut(s) 515
OliI CACNNNNGTG 1 cut(s) 290
PagI TCATGA 1 cut(s) 523
PciI ACATGT 1 cut(s) 511
PfeI GAWTC 3 cut(s) 131, 197, 720
PflFI GACNNNGTC 1 cut(s) 612
PkrI GCNGC 1 cut(s) 207
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
PscI ACATGT 1 cut(s) 511
PshBI ATTAAT 2 cut(s) 237, 981
Psp124BI GAGCTC 1 cut(s) 793
Psp6I CCWGG 2 cut(s) 75, 200
PspGI CCWGG 2 cut(s) 75, 200
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 2 cut(s) 577, 608
PstI CTGCAG 1 cut(s) 955
PsuI RGATCY 1 cut(s) 1012
PsyI GACNNNGTC 1 cut(s) 612
RsaI GTAC 4 cut(s) 284, 681, 691, 703
RsaNI GTAC 4 cut(s) 283, 680, 690, 702
RseI CAYNNNNRTG 3 cut(s) 290, 500, 983
Rsr2I CGGWCCG 1 cut(s) 608
RsrII CGGWCCG 1 cut(s) 608
SacI GAGCTC 1 cut(s) 793
SaqAI TTAA 6 cut(s) 60, 87, 237, 371, 564, 981
SatI GCNGC 1 cut(s) 206
Sau3AI GATC 6 cut(s) 231, 361, 811, 919, 1012, 1144
Sau96I GGNCC 2 cut(s) 577, 608
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 4 cut(s) 77, 202, 612, 735
SduI GDGCHC 1 cut(s) 793
SfaNI GCATC 2 cut(s) 1105, 1120
SfcI CTRYAG 1 cut(s) 951
SinI GGWCC 2 cut(s) 577, 608
SmiMI CAYNNNNRTG 3 cut(s) 290, 500, 983
SmlI CTYRAG 1 cut(s) 171
SmoI CTYRAG 1 cut(s) 171
Sse9I AATT 8 cut(s) 138, 150, 352, 534, 621, 685, 859, 1102
SsiI CCGC 3 cut(s) 606, 843, 914
SspI AATATT 1 cut(s) 520
SspMI CTAG 3 cut(s) 717, 965, 1098
SstI GAGCTC 1 cut(s) 793
StyD4I CCNGG 4 cut(s) 75, 200, 610, 733
StyI CCWWGG 1 cut(s) 278
TaaI ACNGT 2 cut(s) 104, 664
TaiI ACGT 2 cut(s) 390, 857
TaqI TCGA 2 cut(s) 857, 901
TaqII GACCGA 1 cut(s) 565
TasI AATT 8 cut(s) 138, 150, 352, 534, 621, 685, 859, 1102
TfiI GAWTC 3 cut(s) 131, 197, 720
Tru1I TTAA 6 cut(s) 60, 87, 237, 371, 564, 981
Tru9I TTAA 6 cut(s) 60, 87, 237, 371, 564, 981
TscAI CASTG 2 cut(s) 873, 1084
TseFI GTSAC 1 cut(s) 883
TseI GCWGC 1 cut(s) 205
Tsp45I GTSAC 1 cut(s) 883
TspDTI ATGAA 6 cut(s) 261, 440, 512, 722, 1001, 1073
TspRI CASTG 2 cut(s) 873, 1084
Tth111I GACNNNGTC 1 cut(s) 612
VpaK11BI GGWCC 2 cut(s) 577, 608
VspI ATTAAT 2 cut(s) 237, 981
XapI RAATTY 3 cut(s) 138, 859, 1102
XceI RCATGY 1 cut(s) 515
XspI CTAG 3 cut(s) 717, 965, 1098
Zsp2I ATGCAT 2 cut(s) 38, 1135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.