FvH4_4g07180

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000579
Physical Location & Seq
Forward (+)
6597223 .. 6600708
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g07180.t1

Sequence Viewer

Length: 1323 bp
ATGGCCAACTCGAGTGAGACTATAAAAGGGCTAAAAAGAATCAGGTATATATCCATTGTAGTACTAGGTCTTCCTTCACTACTTCACTCTCTTGTGTTTTTATGGATTGTTGCTTCGTCTACTTTTGGCGTTGGTTATGGCCAAAATGGTACTCTCAACCTCCTTGATTTCGGCGCCTCCGGCAATGGCGTAGCTGACGATACTGAAGCTTTCGACACTGCATGGAAGACGCTTTGCTCTACCGGCCAAACCTTAAACATCCCTGCCGGGAAGACCTTCGTAGTTAAACCTCTTAAACTTGAAGGTCCTTGCAAATCCAGTGGTGTTGTAATTCAGATTGATGGGAATCTTGTTGGTCCGCCCACTCTTGGCGAATGGCAAGACGCGTGCGCACTGAAATGTTGGCTATGCTTCATGAGCGTGAACGGCCTCGTCATCAAGGGGAAAGGAGTTATAGATGGCAAGGGCTCAATTTGGTGGAACCAAAAAACGCAATCCATAGATGAGGCTGCAAACAGTTGCAAAGCACCCACGGCTTTATACTTCAACAAATGCTATCAACTTCAATTATCAGGAATTACTTCTCGTGATAGCCCTAGAGTCCATATATTTTTATTTCGCAGCAATGGTGCACATATATCAAACGTTCATGTGAGCGCACCGGAAAATAGCCCGAATACTGATGGCATCGACGTCTCTGAATCAAGCCAAGTAACCATACAAGACTCTGTTATCGGAACTGGTGATGATTGCGTTGCAATTAAGGGAGGCGCCTCTTTTGTAAACGTAACTCATGTGACATGCGGACCAGGTCATGGTTTCAGTGTCGGTAGTTTAGGGATGATTCCATCTACTGCAGATGAAGTCCATGACATATATTTTCAGAATTGTAACTGTGACAAAACTATGAACTGCGCAAGAATTAAAACATGGATGGGAGGATCTGGGTTTGCCAAAGAGATTTTCTATAATCAGATGACCCTTCAACAGTCACAAAATCCAATTTTGATTGACCAACACTACTGTGATGGCGGACATAGTTGCACAGTTATGGCCGGAGCGGTGAAAGTTAGTGACATAACATTCAGTGGCTTTACCGGAAGTTCAGCAAGTGAGCAAGCAATAACATTGAATTGCTCGGACTCGGGTTGCCCCAATATTCTGTTGGATCATGTCAGCCTAACTTCTGGGATCGGAAAGCCACTTCGTTCTGCATGTTACAAGGCTACTGGAAAAGCTAGTTTTACTGATCCTCCAGTTCCTTGCCTAAACTCGAAGCAAAATCTGGCACCAGTGATGGCACCACTCCCAATCACATATTAA

Protein Analysis

441

Amino Acids

46.63

Weight (kDa)

6.35

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 53 - 264 2.9e-06 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 83 - 410 2.2e-83 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 696
Acc16I TGCGCA 2 cut(s) 391, 916
AccB1I GGYRCC 4 cut(s) 173, 770, 1288, 1300
AccB7I CCANNNNNTGG 1 cut(s) 815
AccBSI CCGCTC 1 cut(s) 1061
AccI GTMKAC 1 cut(s) 119
AccII CGCG 1 cut(s) 386
AciI CCGC 4 cut(s) 359, 804, 1032, 1061
AclI AACGTT 1 cut(s) 645
AclWI GGATC 4 cut(s) 949, 1176, 1199, 1244
AcoI YGGCCR 4 cut(s) 3, 139, 244, 1053
AcuI CTGAAG 1 cut(s) 225
AcyI GRCGYC 3 cut(s) 174, 693, 771
AfaI GTAC 2 cut(s) 63, 151
AfiI CCNNNNNNNGG 2 cut(s) 368, 815
AflIII ACRYGT 1 cut(s) 384
AgsI TTSAA 5 cut(s) 302, 547, 566, 986, 1132
AjnI CCWGG 1 cut(s) 808
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AleI CACNNNNGTG 1 cut(s) 1023
AluBI AGCT 3 cut(s) 194, 209, 1238
AluI AGCT 3 cut(s) 194, 209, 1238
Alw21I GWGCWC 1 cut(s) 634
Alw26I GTCTC 2 cut(s) 11, 700
Alw44I GTGCAC 1 cut(s) 630
AlwI GGATC 4 cut(s) 949, 1176, 1199, 1244
Ama87I CYCGRG 2 cut(s) 10, 1144
AoxI GGCC 5 cut(s) 3, 139, 244, 427, 1053
ApaLI GTGCAC 1 cut(s) 630
ApeKI GCWGC 2 cut(s) 509, 621
Asp700I GAANNNNTTC 2 cut(s) 275, 580
AspLEI GCGC 5 cut(s) 176, 392, 659, 773, 917
AspS9I GGNCC 3 cut(s) 305, 356, 806
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 755, 1075
AvaI CYCGRG 2 cut(s) 10, 1144
AvaII GGWCC 3 cut(s) 305, 356, 806
BaeGI GKGCMC 1 cut(s) 634
BalI TGGCCA 2 cut(s) 5, 141
BanI GGYRCC 4 cut(s) 173, 770, 1288, 1300
BanII GRGCYC 1 cut(s) 470
BarI GAAGNNNNNNTAC 2 cut(s) 54, 86
BauI CACGAG 1 cut(s) 585
BbsI GAAGAC 3 cut(s) 62, 233, 278
Bbv12I GWGCWC 1 cut(s) 634
BbvI GCAGC 2 cut(s) 496, 633
BccI CCATC 7 cut(s) 335, 452, 677, 856, 928, 1022, 1291
BceAI ACGGC 2 cut(s) 442, 549
BciT130I CCWGG 1 cut(s) 810
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 11, 700
BfaI CTAG 3 cut(s) 65, 597, 1239
BfmI CTRYAG 1 cut(s) 855
BfoI RGCGCY 2 cut(s) 177, 774
BisI GCNGC 2 cut(s) 510, 622
BlsI GCNGC 2 cut(s) 511, 623
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 2 cut(s) 268, 810
Bme18I GGWCC 3 cut(s) 305, 356, 806
BmeT110I CYCGRG 2 cut(s) 10, 1144
BmgT120I GGNCC 3 cut(s) 305, 356, 806
BmiI GGNNCC 5 cut(s) 175, 482, 772, 1290, 1302
BmrFI CCNGG 2 cut(s) 268, 810
BmsI GCATC 1 cut(s) 696
BpiI GAAGAC 3 cut(s) 62, 233, 278
BpmI CTGGAG 1 cut(s) 1239
BpuMI CCSGG 1 cut(s) 268
BsaBI GATNNNNATC 1 cut(s) 345
BsaHI GRCGYC 3 cut(s) 174, 693, 771
BsaJI CCNNGG 1 cut(s) 531
BsaWI WCCGGW 2 cut(s) 661, 1097
BsaXI ACNNNNNCTCC 2 cut(s) 1237, 1267
Bsc4I CCNNNNNNNGG 2 cut(s) 368, 815
Bse118I RCCGGY 1 cut(s) 242
Bse1I ACTGG 5 cut(s) 318, 745, 1234, 1256, 1292
Bse3DI GCAATG 2 cut(s) 190, 631
Bse8I GATNNNNATC 1 cut(s) 345
BseBI CCWGG 1 cut(s) 810
BseDI CCNNGG 1 cut(s) 531
BseGI GGATG 3 cut(s) 258, 846, 939
BseJI GATNNNNATC 1 cut(s) 345
BseLI CCNNNNNNNGG 2 cut(s) 368, 815
BseMI GCAATG 2 cut(s) 190, 631
BseNI ACTGG 5 cut(s) 318, 745, 1234, 1256, 1292
BseSI GKGCMC 1 cut(s) 634
BseXI GCAGC 2 cut(s) 496, 633
Bsh1236I CGCG 1 cut(s) 386
BshFI GGCC 5 cut(s) 5, 141, 246, 429, 1055
BshNI GGYRCC 4 cut(s) 173, 770, 1288, 1300
BsiHKAI GWGCWC 1 cut(s) 634
BsiHKCI CYCGRG 2 cut(s) 10, 1144
BsiSI CCGG 6 cut(s) 180, 243, 267, 662, 1056, 1098
BslI CCNNNNNNNGG 2 cut(s) 368, 815
BsmAI GTCTC 2 cut(s) 11, 700
BsmBI CGTCTC 1 cut(s) 700
BsnI GGCC 5 cut(s) 5, 141, 246, 429, 1055
BsoBI CYCGRG 2 cut(s) 10, 1144
Bsp1286I GDGCHC 2 cut(s) 470, 634
Bsp143I GATC 4 cut(s) 941, 1168, 1191, 1249
BspACI CCGC 4 cut(s) 359, 804, 1032, 1061
BspANI GGCC 5 cut(s) 5, 141, 246, 429, 1055
BspFNI CGCG 1 cut(s) 386
BspHI TCATGA 1 cut(s) 414
BspLI GGNNCC 5 cut(s) 175, 482, 772, 1290, 1302
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 4 cut(s) 949, 1176, 1199, 1244
BspT107I GGYRCC 4 cut(s) 173, 770, 1288, 1300
BsrBI CCGCTC 1 cut(s) 1061
BsrDI GCAATG 2 cut(s) 190, 631
BsrFI RCCGGY 1 cut(s) 242
BsrI ACTGG 5 cut(s) 318, 745, 1234, 1256, 1292
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 531
BssMI GATC 4 cut(s) 941, 1168, 1191, 1249
BssNI GRCGYC 3 cut(s) 174, 693, 771
BssSI CACGAG 1 cut(s) 585
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 810
Bst4CI ACNGT 5 cut(s) 518, 896, 990, 1025, 1048
BstACI GRCGYC 3 cut(s) 174, 693, 771
BstC8I GCNNGC 2 cut(s) 388, 1119
BstDSI CCRYGG 1 cut(s) 531
BstF5I GGATG 3 cut(s) 258, 846, 939
BstFNI CGCG 1 cut(s) 386
BstH2I RGCGCY 2 cut(s) 177, 774
BstHHI GCGC 5 cut(s) 176, 392, 659, 773, 917
BstKTI GATC 4 cut(s) 944, 1171, 1194, 1252
BstMAI GTCTC 2 cut(s) 11, 700
BstMBI GATC 4 cut(s) 941, 1168, 1191, 1249
BstMWI GCNNNNNNNGC 5 cut(s) 180, 243, 417, 426, 533
BstNI CCWGG 1 cut(s) 810
BstNSI RCATGY 2 cut(s) 804, 1218
BstSCI CCNGG 2 cut(s) 266, 808
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 634
BstUI CGCG 1 cut(s) 386
BstV1I GCAGC 2 cut(s) 496, 633
BstV2I GAAGAC 3 cut(s) 62, 233, 278
BstX2I RGATCY 1 cut(s) 941
BstYI RGATCY 1 cut(s) 941
BsuRI GGCC 5 cut(s) 5, 141, 246, 429, 1055
BtgI CCRYGG 1 cut(s) 531
BtsCI GGATG 3 cut(s) 258, 846, 939
BtsI GCAGTG 1 cut(s) 216
BtsIMutI CAGTG 6 cut(s) 216, 325, 392, 829, 1093, 1299
Cac8I GCNNGC 2 cut(s) 388, 1119
CciI TCATGA 1 cut(s) 414
CfoI GCGC 5 cut(s) 176, 392, 659, 773, 917
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 305, 356, 806
CseI GACGC 2 cut(s) 238, 392
CsiI ACCWGGT 1 cut(s) 808
Csp6I GTAC 2 cut(s) 62, 150
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviQI GTAC 2 cut(s) 62, 150
DinI GGCGCC 2 cut(s) 175, 772
DpnI GATC 4 cut(s) 943, 1170, 1193, 1251
DpnII GATC 4 cut(s) 941, 1168, 1191, 1249
EaeI YGGCCR 4 cut(s) 3, 139, 244, 1053
EciI GGCGGA 2 cut(s) 348, 1047
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 3 cut(s) 305, 356, 806
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 2 cut(s) 10, 1144
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 808
EcoT38I GRGCYC 1 cut(s) 470
EgeI GGCGCC 2 cut(s) 175, 772
EheI GGCGCC 2 cut(s) 175, 772
Esp3I CGTCTC 1 cut(s) 700
FblI GTMKAC 1 cut(s) 119
Fnu4HI GCNGC 2 cut(s) 510, 622
FokI GGATG 3 cut(s) 245, 853, 946
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 2 cut(s) 510, 622
FspAI RTGCGCAY 1 cut(s) 391
FspBI CTAG 3 cut(s) 65, 597, 1239
FspI TGCGCA 2 cut(s) 391, 916
GlaI GCGC 5 cut(s) 175, 391, 658, 772, 916
GluI GCNGC 2 cut(s) 510, 622
GsuI CTGGAG 1 cut(s) 1239
HaeII RGCGCY 2 cut(s) 177, 774
HaeIII GGCC 5 cut(s) 5, 141, 246, 429, 1055
HapII CCGG 6 cut(s) 180, 243, 267, 662, 1056, 1098
HgaI GACGC 2 cut(s) 238, 392
HhaI GCGC 5 cut(s) 176, 392, 659, 773, 917
Hin1I GRCGYC 3 cut(s) 174, 693, 771
Hin6I GCGC 5 cut(s) 174, 390, 657, 771, 915
HinP1I GCGC 5 cut(s) 174, 390, 657, 771, 915
HindIII AAGCTT 1 cut(s) 207
HinfI GANTC 7 cut(s) 39, 346, 600, 701, 725, 844, 1142
HpaII CCGG 6 cut(s) 180, 243, 267, 662, 1056, 1098
HphI GGTGA 2 cut(s) 755, 1075
Hpy166II GTNNAC 4 cut(s) 120, 424, 632, 784
Hpy188I TCNGA 7 cut(s) 336, 700, 737, 885, 975, 1141, 1196
Hpy188III TCNNGA 3 cut(s) 415, 573, 587
Hpy8I GTNNAC 4 cut(s) 120, 424, 632, 784
Hpy99I CGWCG 1 cut(s) 695
HpyAV CCTTC 4 cut(s) 84, 286, 296, 992
HpyCH4III ACNGT 5 cut(s) 518, 896, 990, 1025, 1048
HpyCH4IV ACGT 3 cut(s) 645, 693, 786
HpyCH4V TGCA 9 cut(s) 221, 312, 512, 522, 632, 758, 857, 1044, 1214
HpyF10VI GCNNNNNNNGC 5 cut(s) 180, 243, 417, 426, 533
HpySE526I ACGT 3 cut(s) 645, 693, 786
Hsp92I GRCGYC 3 cut(s) 174, 693, 771
HspAI GCGC 5 cut(s) 174, 390, 657, 771, 915
KasI GGCGCC 2 cut(s) 173, 770
Kzo9I GATC 4 cut(s) 941, 1168, 1191, 1249
LmnI GCTCC 1 cut(s) 1058
Lsp1109I GCAGC 2 cut(s) 496, 633
LweI GCATC 1 cut(s) 696
MabI ACCWGGT 1 cut(s) 808
MaeI CTAG 3 cut(s) 65, 597, 1239
MaeII ACGT 3 cut(s) 645, 693, 786
MaeIII GTNAC 8 cut(s) 712, 787, 796, 890, 896, 990, 1073, 1217
MalI GATC 4 cut(s) 943, 1170, 1193, 1251
MbiI CCGCTC 1 cut(s) 1061
MboI GATC 4 cut(s) 941, 1168, 1191, 1249
MboII GAAGA 3 cut(s) 62, 238, 283
MflI RGATCY 1 cut(s) 941
MhlI GDGCHC 2 cut(s) 470, 634
MlsI TGGCCA 2 cut(s) 5, 141
MluCI AATT 9 cut(s) 330, 471, 566, 576, 759, 886, 921, 1002, 1132
MluI ACGCGT 1 cut(s) 384
MluNI TGGCCA 2 cut(s) 5, 141
Mly113I GGCGCC 2 cut(s) 174, 771
MlyI GAGTC 3 cut(s) 609, 719, 1136
MmeI TCCRAC 1 cut(s) 1146
MnlI CCTC 9 cut(s) 170, 187, 300, 440, 499, 761, 784, 932, 1263
Mox20I TGGCCA 2 cut(s) 5, 141
MroXI GAANNNNTTC 2 cut(s) 275, 580
MscI TGGCCA 2 cut(s) 5, 141
MseI TTAA 6 cut(s) 254, 285, 294, 762, 924, 1321
MslI CAYNNNNRTG 4 cut(s) 397, 419, 1023, 1049
Msp20I TGGCCA 2 cut(s) 5, 141
MspI CCGG 6 cut(s) 180, 243, 267, 662, 1056, 1098
MspR9I CCNGG 2 cut(s) 268, 810
MvaI CCWGG 1 cut(s) 810
MvnI CGCG 1 cut(s) 386
MwoI GCNNNNNNNGC 5 cut(s) 180, 243, 417, 426, 533
NarI GGCGCC 2 cut(s) 174, 771
NciI CCSGG 1 cut(s) 268
NdeII GATC 4 cut(s) 941, 1168, 1191, 1249
NlaIV GGNNCC 5 cut(s) 175, 482, 772, 1290, 1302
NmuCI GTSAC 4 cut(s) 796, 896, 990, 1073
NsbI TGCGCA 2 cut(s) 391, 916
NspI RCATGY 2 cut(s) 804, 1218
OliI CACNNNNGTG 1 cut(s) 1023
PaeR7I CTCGAG 1 cut(s) 10
PagI TCATGA 1 cut(s) 414
PdmI GAANNNNTTC 2 cut(s) 275, 580
PfeI GAWTC 4 cut(s) 39, 346, 701, 844
PflFI GACNNNGTC 1 cut(s) 810
PflMI CCANNNNNTGG 1 cut(s) 815
PkrI GCNGC 2 cut(s) 511, 623
PleI GAGTC 3 cut(s) 608, 719, 1136
PluTI GGCGCC 2 cut(s) 177, 774
PpsI GAGTC 3 cut(s) 608, 719, 1136
PpuMI RGGWCCY 1 cut(s) 305
Psp1406I AACGTT 1 cut(s) 645
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 808
PspGI CCWGG 1 cut(s) 808
PspN4I GGNNCC 5 cut(s) 175, 482, 772, 1290, 1302
PspPI GGNCC 3 cut(s) 305, 356, 806
PspPPI RGGWCCY 1 cut(s) 305
PspXI VCTCGAGB 1 cut(s) 10
PstI CTGCAG 1 cut(s) 859
PsuI RGATCY 1 cut(s) 941
PsyI GACNNNGTC 1 cut(s) 810
RsaI GTAC 2 cut(s) 63, 151
RsaNI GTAC 2 cut(s) 62, 150
RseI CAYNNNNRTG 4 cut(s) 397, 419, 1023, 1049
SaqAI TTAA 6 cut(s) 254, 285, 294, 762, 924, 1321
SatI GCNGC 2 cut(s) 510, 622
Sau3AI GATC 4 cut(s) 941, 1168, 1191, 1249
Sau96I GGNCC 3 cut(s) 305, 356, 806
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 3 cut(s) 609, 719, 1136
ScrFI CCNGG 2 cut(s) 268, 810
SduI GDGCHC 2 cut(s) 470, 634
SexAI ACCWGGT 1 cut(s) 808
SfaNI GCATC 1 cut(s) 696
SfcI CTRYAG 1 cut(s) 855
SfoI GGCGCC 2 cut(s) 175, 772
Sfr274I CTCGAG 1 cut(s) 10
SinI GGWCC 3 cut(s) 305, 356, 806
SlaI CTCGAG 1 cut(s) 10
SmiMI CAYNNNNRTG 4 cut(s) 397, 419, 1023, 1049
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 9 cut(s) 330, 471, 566, 576, 759, 886, 921, 1002, 1132
SsiI CCGC 4 cut(s) 359, 804, 1032, 1061
SspDI GGCGCC 2 cut(s) 173, 770
SspI AATATT 1 cut(s) 1159
SspMI CTAG 3 cut(s) 65, 597, 1239
StyD4I CCNGG 2 cut(s) 266, 808
TaaI ACNGT 5 cut(s) 518, 896, 990, 1025, 1048
TaiI ACGT 3 cut(s) 648, 696, 789
TaqI TCGA 4 cut(s) 11, 213, 690, 1274
TasI AATT 9 cut(s) 330, 471, 566, 576, 759, 886, 921, 1002, 1132
TatI WGTACW 1 cut(s) 61
TfiI GAWTC 4 cut(s) 39, 346, 701, 844
Tru1I TTAA 6 cut(s) 254, 285, 294, 762, 924, 1321
Tru9I TTAA 6 cut(s) 254, 285, 294, 762, 924, 1321
TscAI CASTG 6 cut(s) 223, 325, 399, 829, 1093, 1299
TseFI GTSAC 4 cut(s) 796, 896, 990, 1073
TseI GCWGC 2 cut(s) 509, 621
Tsp45I GTSAC 4 cut(s) 796, 896, 990, 1073
TspDTI ATGAA 4 cut(s) 403, 638, 876, 923
TspRI CASTG 6 cut(s) 223, 325, 399, 829, 1093, 1299
Tth111I GACNNNGTC 1 cut(s) 810
Van91I CCANNNNNTGG 1 cut(s) 815
VneI GTGCAC 1 cut(s) 630
VpaK11BI GGWCC 3 cut(s) 305, 356, 806
XceI RCATGY 2 cut(s) 804, 1218
XcmI CCANNNNNNNNNTGG 1 cut(s) 1162
XhoI CTCGAG 1 cut(s) 10
XmiI GTMKAC 1 cut(s) 119
XmnI GAANNNNTTC 2 cut(s) 275, 580
XspI CTAG 3 cut(s) 65, 597, 1239
ZraI GACGTC 1 cut(s) 694
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.