RLG00000009272

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000579
Physical Location & Seq
Reverse (-)
50000024 .. 50006895
6872 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009272

Sequence Viewer

Length: 2040 bp
ATGGAGTTGCCAGGATCCAAGCCTTTCATCTACGGCTTCCATGGCCACTGTTTAAGCCAAAGTGTATCATCATCCTCTATGGATGTTAGCGTCGTGCCAGATGGCAACATGCTGACGGAGTTGTTAGATCCCTACCCGAAGTCGATCGGCGATCAGTTAACGCATCCGGTGGTTCAGATTTCATCGGTGGAACGAGTAGCTAAGGTGTTGAGTCATGTACCTTCGACGGGAGGTAGCGCTCTCCTTCCTTTTCCCCTTTTCCGTGGTGGTGACAACGACGGCGGTATAGGTGGTATTGTCGGTGACAAAGATGGTGGTATGGGTTTGTGTTCTGGGAGTACGATCGGTGGTGGAGATAGTGTTCCCTCAGATTTGGATTGTTGGGATTCGGGGAGGCTTGCCAGTGGGATGGATTTGTTCTACGTTGGGTTGGCTTGGGACTGGGATGTAAGCTGGACGAAGATTGCTAGAGCGGGCATCTGTGTCGGTGCCTGGCTTGGTGATGATGATATCTGGCTGGTGACGTCAACAGAGCCGTTGTGCTTGAGGCCAGGTGCTACGCGGCTTTTCTTTGCTCGGGTTGGTGTTAACCTCGGCTCCGGTGGTGGCAACGGCGACATAGGGGGCGGTGATATGTGCTGGGGTGTCTCTTCTACTTTCGGTGGCAGAGGCGGTGGCTGGCAGATGACGGCGGCGGGGAGGATGGTCGCTGGGCTTCTAGGGTTTACTCTAGTGGCTTTTAGTGGGTCAGGGCTCATTGGCGCTTGGGCCTTGAAGGCTGTTTGGCTGGGTCTACTTTCGCTACTTACCTCTGTGTTGTTTTCATGGATTGTTGCTTCACCTAGATTTGGCATTGGGCCATGGTATGGCCAAAATGGTACTCTTAACGTACTTGATTTTGGTGCCTCTGGTGATGGCATAGCAGATGATACTGAAGCTTTCTTAAATGCATGGAAGACACTTTGCGAGACCGGCCAAACCCTAAACATCCCTGCAGGGAAGACCTTTGTAGTCAAACCTCTTCAACTTGTAGGTCCATGCAAATCCAATAACGTTGGAATTCAGATTGATGGGAATATTGTCGGTCCATCCACTCTTAGCGAATGGGCAGACGCGTGCAAAATCGGACAATGTTGGCTATGCTTTGTAAACGTGAACGGCCTCGTCGTCAAGGGGAAAGGACTTATAAATGGCAATGGTCCAATTTGGTGGAACCAGGAAACCCAATCCATAAATGCTTTAAACTTCCACAAATGCGACCAACTTCAATTATCTGGATTTACTTCCCGTGATAGTCCGAGAGCCCATATATTTATACATAGCAGCAATAATGTACGTATCTCAAACATTCACTTGAGTGCACCGGAAAAAAGCCCGAATACTGATGGCATAGACATCTCTTTATCAAGCCAAGTAACCATACAAGACTCTTTCATCGGAACTGGTGATGACTGCATTGCAATTAAGGGAGGCTCCTCTTTTGTAAACATAACTCATGTAACATGCGGACCAGGTCATGGTTTCAGTGTGGGTAGTTTAGGACAAGATCCAGCTACTACGGATAATGTCGAACAAGTATACTTTCAGAATTGTATCTGCAACAAATCTACAAACTGTGCAAGAATCAAAACATGGATGGGTGGATCTGGGTATGCTCGGGCGATTTTCTTCAGACAAATGACGCTTGAACAATCACAAAATCCTATTATAATTGACCAACACTACTGCAATGGCGGACATAATTGCCCAGCTAAGACGGGAGCAGTGAAAGTTAGCAATGTAACATTCAGTGGCATTACCGGAACTTCAGCAAGTGAGCAAGCAATAACACTGGATTGCTCCGACTTGGGTTGTACCGATATCTTGATGGATCATGTCAACATAACCTCCGCAGCCGGACTGCCACTTCGTTCTGTGTGCAACAAGGCCAGTGGGAAAGCTAGTTTTACTTCACCTCCGATTCCTTGCCTGTCGTCGAATGAATCTTTGTCATCAGTGATTGCACCAATCTCGTCCTATGAACCGGACCCAATTAAATAA

Protein Analysis

680

Amino Acids

71.15

Weight (kDa)

5.27

Isoelectric Point (pI)

34.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 295 - 516 2.7e-11 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 326 - 644 2.2e-78 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1187, 1709
AatII GACGTC 1 cut(s) 527
AccB1I GGYRCC 2 cut(s) 488, 902
AccB7I CCANNNNNTGG 2 cut(s) 866, 1517
AccBSI CCGCTC 1 cut(s) 473
AccI GTMKAC 2 cut(s) 793, 1578
AccII CGCG 2 cut(s) 562, 1115
AclI AACGTT 1 cut(s) 1053
AclWI GGATC 6 cut(s) 9, 22, 122, 1541, 1651, 1878
AcoI YGGCCR 3 cut(s) 43, 868, 973
AcsI RAATTY 1 cut(s) 1059
AcuI CTGAAG 3 cut(s) 954, 1654, 1791
AcyI GRCGYC 1 cut(s) 524
AfaI GTAC 6 cut(s) 219, 340, 880, 891, 1335, 1855
AfeI AGCGCT 1 cut(s) 238
AfiI CCNNNNNNNGG 4 cut(s) 227, 848, 866, 1517
AflIII ACRYGT 1 cut(s) 1113
AgsI TTSAA 4 cut(s) 775, 1025, 1268, 1688
AjnI CCWGG 5 cut(s) 10, 491, 550, 1215, 1510
AleI CACNNNNGTG 1 cut(s) 1356
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 6 cut(s) 200, 453, 938, 1553, 1751, 1940
AluI AGCT 6 cut(s) 200, 453, 938, 1553, 1751, 1940
Alw21I GWGCWC 1 cut(s) 1363
Alw26I GTCTC 2 cut(s) 652, 962
Alw44I GTGCAC 1 cut(s) 1359
AlwI GGATC 6 cut(s) 9, 22, 122, 1541, 1651, 1878
Ama87I CYCGRG 2 cut(s) 576, 1656
Aor51HI AGCGCT 1 cut(s) 238
AoxI GGCC 8 cut(s) 43, 548, 768, 857, 868, 973, 1159, 1926
ApaLI GTGCAC 1 cut(s) 1359
ApeKI GCWGC 2 cut(s) 1323, 1892
ApoI RAATTY 1 cut(s) 1059
ArsI GACNNNNNNTTYG 2 cut(s) 1890, 1922
AspLEI GCGC 2 cut(s) 239, 764
AspS9I GGNCC 7 cut(s) 768, 857, 1034, 1085, 1199, 1508, 2026
AsuHPI GGTGA 9 cut(s) 281, 314, 512, 532, 641, 831, 923, 1457, 1944
AvaI CYCGRG 2 cut(s) 576, 1656
AvaII GGWCC 5 cut(s) 1034, 1085, 1199, 1508, 2026
BaeGI GKGCMC 1 cut(s) 1363
BalI TGGCCA 2 cut(s) 45, 870
BamHI GGATCC 1 cut(s) 14
BanI GGYRCC 2 cut(s) 488, 902
BanII GRGCYC 2 cut(s) 756, 1306
BbsI GAAGAC 2 cut(s) 962, 1007
Bbv12I GWGCWC 1 cut(s) 1363
BbvI GCAGC 2 cut(s) 1335, 1904
BceAI ACGGC 6 cut(s) 49, 295, 520, 628, 705, 1174
BcgI CGANNNNNNTGC 4 cut(s) 466, 500, 1992, 2026
BciT130I CCWGG 5 cut(s) 12, 493, 552, 1217, 1512
BcoDI GTCTC 2 cut(s) 652, 962
BfaI CTAG 5 cut(s) 468, 719, 731, 843, 1941
BfmI CTRYAG 1 cut(s) 993
BfoI RGCGCY 2 cut(s) 240, 765
BglI GCCNNNNNGGC 1 cut(s) 776
BisI GCNGC 4 cut(s) 563, 693, 1324, 1893
BlsI GCNGC 4 cut(s) 564, 694, 1325, 1894
Bme1390I CCNGG 5 cut(s) 12, 493, 552, 1217, 1512
Bme18I GGWCC 5 cut(s) 1034, 1085, 1199, 1508, 2026
BmeT110I CYCGRG 2 cut(s) 576, 1656
BmgT120I GGNCC 7 cut(s) 768, 857, 1034, 1085, 1199, 1508, 2026
BmiI GGNNCC 7 cut(s) 16, 490, 598, 904, 1214, 1474, 2028
BmrFI CCNGG 5 cut(s) 12, 493, 552, 1217, 1512
BmrI ACTGGG 1 cut(s) 451
BmsI GCATC 2 cut(s) 172, 486
BmuI ACTGGG 1 cut(s) 451
BpiI GAAGAC 2 cut(s) 962, 1007
Bpu10I CCTNAGC 1 cut(s) 201
BpuEI CTTGAG 2 cut(s) 565, 1375
BsaAI YACGTR 1 cut(s) 1337
BsaHI GRCGYC 1 cut(s) 524
BsaI GGTCTC 1 cut(s) 962
BsaJI CCNNGG 4 cut(s) 40, 262, 592, 860
BsaWI WCCGGW 5 cut(s) 166, 599, 1363, 1799, 2023
BsaXI ACNNNNNCTCC 8 cut(s) 345, 375, 581, 611, 1871, 1901, 1939, 1969
Bsc4I CCNNNNNNNGG 4 cut(s) 227, 848, 866, 1517
Bse118I RCCGGY 1 cut(s) 971
Bse1I ACTGG 5 cut(s) 402, 446, 1447, 1836, 1929
Bse3DI GCAATG 4 cut(s) 1201, 1455, 1735, 1783
BseBI CCWGG 5 cut(s) 12, 493, 552, 1217, 1512
BseDI CCNNGG 4 cut(s) 40, 262, 592, 860
BseGI GGATG 9 cut(s) 71, 88, 163, 414, 451, 708, 987, 1088, 1641
BseLI CCNNNNNNNGG 4 cut(s) 227, 848, 866, 1517
BseMI GCAATG 4 cut(s) 1201, 1455, 1735, 1783
BseMII CTCAG 1 cut(s) 381
BseNI ACTGG 5 cut(s) 402, 446, 1447, 1836, 1929
BseRI GAGGAG 1 cut(s) 1465
BseSI GKGCMC 1 cut(s) 1363
BseXI GCAGC 2 cut(s) 1335, 1904
BseYI CCCAGC 4 cut(s) 639, 710, 787, 1747
Bsh1236I CGCG 2 cut(s) 562, 1115
Bsh1285I CGRYCG 2 cut(s) 147, 345
BshFI GGCC 8 cut(s) 45, 550, 770, 859, 870, 975, 1161, 1928
BshNI GGYRCC 2 cut(s) 488, 902
BsiEI CGRYCG 2 cut(s) 147, 345
BsiHKAI GWGCWC 1 cut(s) 1363
BsiHKCI CYCGRG 2 cut(s) 576, 1656
BsiSI CCGG 7 cut(s) 167, 600, 972, 1364, 1800, 1896, 2024
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 4 cut(s) 227, 848, 866, 1517
BsmAI GTCTC 2 cut(s) 652, 962
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 8 cut(s) 45, 550, 770, 859, 870, 975, 1161, 1928
Bso31I GGTCTC 1 cut(s) 962
BsoBI CYCGRG 2 cut(s) 576, 1656
Bsp1286I GDGCHC 3 cut(s) 756, 1306, 1363
Bsp143I GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
Bsp19I CCATGG 2 cut(s) 40, 860
BspANI GGCC 8 cut(s) 45, 550, 770, 859, 870, 975, 1161, 1928
BspCNI CTCAG 1 cut(s) 380
BspFNI CGCG 2 cut(s) 562, 1115
BspLI GGNNCC 7 cut(s) 16, 490, 598, 904, 1214, 1474, 2028
BspMAI CTGCAG 1 cut(s) 997
BspPI GGATC 6 cut(s) 9, 22, 122, 1541, 1651, 1878
BspT107I GGYRCC 2 cut(s) 488, 902
BspTNI GGTCTC 1 cut(s) 962
BsrBI CCGCTC 1 cut(s) 473
BsrDI GCAATG 4 cut(s) 1201, 1455, 1735, 1783
BsrFI RCCGGY 1 cut(s) 971
BsrI ACTGG 5 cut(s) 402, 446, 1447, 1836, 1929
BssAI RCCGGY 1 cut(s) 971
BssECI CCNNGG 4 cut(s) 40, 262, 592, 860
BssMI GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
BssNAI GTATAC 1 cut(s) 1579
BssNI GRCGYC 1 cut(s) 524
BssT1I CCWWGG 2 cut(s) 40, 860
Bst1107I GTATAC 1 cut(s) 1579
Bst2UI CCWGG 5 cut(s) 12, 493, 552, 1217, 1512
Bst4CI ACNGT 2 cut(s) 50, 1616
Bst6I CTCTTC 2 cut(s) 655, 1026
BstACI GRCGYC 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 1337
BstC8I GCNNGC 5 cut(s) 399, 475, 680, 1117, 1821
BstDEI CTNAG 4 cut(s) 201, 367, 1097, 1752
BstDSI CCRYGG 3 cut(s) 40, 262, 860
BstF5I GGATG 9 cut(s) 71, 88, 163, 414, 451, 708, 987, 1088, 1641
BstFNI CGCG 2 cut(s) 562, 1115
BstH2I RGCGCY 2 cut(s) 240, 765
BstHHI GCGC 2 cut(s) 239, 764
BstKTI GATC 8 cut(s) 17, 130, 147, 154, 345, 1549, 1646, 1873
BstMAI GTCTC 2 cut(s) 652, 962
BstMBI GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
BstMCI CGRYCG 2 cut(s) 147, 345
BstMWI GCNNNNNNNGC 3 cut(s) 42, 776, 972
BstNI CCWGG 5 cut(s) 12, 493, 552, 1217, 1512
BstNSI RCATGY 2 cut(s) 112, 1506
BstSCI CCNGG 5 cut(s) 10, 491, 550, 1215, 1510
BstSFI CTRYAG 1 cut(s) 993
BstSLI GKGCMC 1 cut(s) 1363
BstSNI TACGTA 1 cut(s) 1337
BstUI CGCG 2 cut(s) 562, 1115
BstV1I GCAGC 2 cut(s) 1335, 1904
BstV2I GAAGAC 2 cut(s) 962, 1007
BstX2I RGATCY 4 cut(s) 14, 127, 1546, 1643
BstXI CCANNNNNNTGG 2 cut(s) 409, 1209
BstYI RGATCY 4 cut(s) 14, 127, 1546, 1643
BstZ17I GTATAC 1 cut(s) 1579
BsuRI GGCC 8 cut(s) 45, 550, 770, 859, 870, 975, 1161, 1928
BtgI CCRYGG 3 cut(s) 40, 262, 860
BtsCI GGATG 9 cut(s) 71, 88, 163, 414, 451, 708, 987, 1088, 1641
BtsI GCAGTG 1 cut(s) 1770
BtsIMutI CAGTG 8 cut(s) 46, 409, 1531, 1770, 1795, 1829, 1936, 2001
Cac8I GCNNGC 5 cut(s) 399, 475, 680, 1117, 1821
CfoI GCGC 2 cut(s) 239, 764
Cfr10I RCCGGY 1 cut(s) 971
Cfr13I GGNCC 7 cut(s) 768, 857, 1034, 1085, 1199, 1508, 2026
CseI GACGC 3 cut(s) 79, 1121, 1690
CsiI ACCWGGT 1 cut(s) 1510
Csp6I GTAC 6 cut(s) 218, 339, 879, 890, 1334, 1854
CspCI CAANNNNNGTGG 4 cut(s) 295, 330, 1912, 1947
CviQI GTAC 6 cut(s) 218, 339, 879, 890, 1334, 1854
DdeI CTNAG 4 cut(s) 201, 367, 1097, 1752
DpnI GATC 8 cut(s) 16, 129, 146, 153, 344, 1548, 1645, 1872
DpnII GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
DraI TTTAAA 1 cut(s) 1242
EaeI YGGCCR 3 cut(s) 43, 868, 973
Eam1104I CTCTTC 2 cut(s) 655, 1026
EarI CTCTTC 2 cut(s) 655, 1026
EciI GGCGGA 1 cut(s) 1749
Eco105I TACGTA 1 cut(s) 1337
Eco130I CCWWGG 2 cut(s) 40, 860
Eco24I GRGCYC 2 cut(s) 756, 1306
Eco31I GGTCTC 1 cut(s) 962
Eco32I GATATC 2 cut(s) 511, 1861
Eco47I GGWCC 5 cut(s) 1034, 1085, 1199, 1508, 2026
Eco47III AGCGCT 1 cut(s) 238
Eco57I CTGAAG 3 cut(s) 954, 1654, 1791
Eco88I CYCGRG 2 cut(s) 576, 1656
EcoRI GAATTC 1 cut(s) 1059
EcoRII CCWGG 5 cut(s) 10, 491, 550, 1215, 1510
EcoRV GATATC 2 cut(s) 511, 1861
EcoT14I CCWWGG 2 cut(s) 40, 860
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 2 cut(s) 756, 1306
ErhI CCWWGG 2 cut(s) 40, 860
FaqI GGGAC 1 cut(s) 452
FauI CCCGC 2 cut(s) 466, 688
FblI GTMKAC 2 cut(s) 793, 1578
Fnu4HI GCNGC 4 cut(s) 563, 693, 1324, 1893
FokI GGATG 9 cut(s) 58, 95, 150, 421, 458, 715, 974, 1075, 1648
FriOI GRGCYC 2 cut(s) 756, 1306
Fsp4HI GCNGC 4 cut(s) 563, 693, 1324, 1893
FspBI CTAG 5 cut(s) 468, 719, 731, 843, 1941
GlaI GCGC 2 cut(s) 238, 763
GluI GCNGC 4 cut(s) 563, 693, 1324, 1893
GsaI CCCAGC 4 cut(s) 643, 714, 791, 1751
HaeII RGCGCY 2 cut(s) 240, 765
HaeIII GGCC 8 cut(s) 45, 550, 770, 859, 870, 975, 1161, 1928
HapII CCGG 7 cut(s) 167, 600, 972, 1364, 1800, 1896, 2024
HgaI GACGC 3 cut(s) 79, 1121, 1690
HhaI GCGC 2 cut(s) 239, 764
Hin1I GRCGYC 1 cut(s) 524
Hin6I GCGC 2 cut(s) 237, 762
HinP1I GCGC 2 cut(s) 237, 762
HincII GTYRAC 4 cut(s) 159, 528, 589, 1879
HindII GTYRAC 4 cut(s) 159, 528, 589, 1879
HindIII AAGCTT 1 cut(s) 936
HinfI GANTC 6 cut(s) 211, 386, 1427, 1623, 1960, 1982
HpaI GTTAAC 2 cut(s) 159, 589
HpaII CCGG 7 cut(s) 167, 600, 972, 1364, 1800, 1896, 2024
HphI GGTGA 9 cut(s) 281, 314, 512, 532, 641, 831, 923, 1457, 1944
Hpy188III TCNNGA 2 cut(s) 1275, 1864
Hpy99I CGWCG 5 cut(s) 95, 229, 281, 1169, 1978
HpyAV CCTTC 3 cut(s) 231, 254, 769
HpyCH4III ACNGT 2 cut(s) 50, 1616
HpyCH4IV ACGT 6 cut(s) 423, 524, 888, 1053, 1152, 1336
HpyF10VI GCNNNNNNNGC 3 cut(s) 42, 776, 972
HpyF3I CTNAG 4 cut(s) 201, 367, 1097, 1752
HpySE526I ACGT 6 cut(s) 423, 524, 888, 1053, 1152, 1336
Hsp92I GRCGYC 1 cut(s) 524
HspAI GCGC 2 cut(s) 237, 762
KspAI GTTAAC 2 cut(s) 159, 589
Kzo9I GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
LmnI GCTCC 4 cut(s) 602, 1478, 1760, 1844
Lsp1109I GCAGC 2 cut(s) 1335, 1904
LweI GCATC 2 cut(s) 172, 486
MabI ACCWGGT 1 cut(s) 1510
MaeI CTAG 5 cut(s) 468, 719, 731, 843, 1941
MaeII ACGT 6 cut(s) 423, 524, 888, 1053, 1152, 1336
MaeIII GTNAC 6 cut(s) 269, 302, 520, 1414, 1498, 1780
MalI GATC 8 cut(s) 16, 129, 146, 153, 344, 1548, 1645, 1872
MbiI CCGCTC 1 cut(s) 473
MboI GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
MboII GAAGA 6 cut(s) 472, 642, 967, 1012, 1013, 1660
MflI RGATCY 4 cut(s) 14, 127, 1546, 1643
MhlI GDGCHC 3 cut(s) 756, 1306, 1363
MlsI TGGCCA 2 cut(s) 45, 870
MluCI AATT 8 cut(s) 1059, 1203, 1268, 1461, 1588, 1710, 1741, 2031
MluI ACGCGT 1 cut(s) 1113
MluNI TGGCCA 2 cut(s) 45, 870
MlyI GAGTC 2 cut(s) 220, 1421
MmeI TCCRAC 2 cut(s) 1036, 1866
Mox20I TGGCCA 2 cut(s) 45, 870
Mph1103I ATGCAT 1 cut(s) 952
MscI TGGCCA 2 cut(s) 45, 870
MseI TTAA 8 cut(s) 53, 158, 588, 885, 944, 1241, 1464, 2034
MslI CAYNNNNRTG 1 cut(s) 1356
Msp20I TGGCCA 2 cut(s) 45, 870
MspI CCGG 7 cut(s) 167, 600, 972, 1364, 1800, 1896, 2024
MspR9I CCNGG 5 cut(s) 12, 493, 552, 1217, 1512
MvaI CCWGG 5 cut(s) 12, 493, 552, 1217, 1512
MvnI CGCG 2 cut(s) 562, 1115
MwoI GCNNNNNNNGC 3 cut(s) 42, 776, 972
NcoI CCATGG 2 cut(s) 40, 860
NdeII GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
NlaIV GGNNCC 7 cut(s) 16, 490, 598, 904, 1214, 1474, 2028
NmeAIII GCCGAG 1 cut(s) 573
NmuCI GTSAC 3 cut(s) 269, 302, 520
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 2 cut(s) 112, 1506
OliI CACNNNNGTG 1 cut(s) 1356
PcsI WCGNNNNNNNCGW 1 cut(s) 1164
PfeI GAWTC 4 cut(s) 386, 1623, 1960, 1982
PflFI GACNNNGTC 1 cut(s) 1512
PflMI CCANNNNNTGG 2 cut(s) 866, 1517
PkrI GCNGC 4 cut(s) 564, 694, 1325, 1894
Ple19I CGATCG 2 cut(s) 147, 345
PleI GAGTC 2 cut(s) 219, 1421
PpsI GAGTC 2 cut(s) 219, 1421
Ppu21I YACGTR 1 cut(s) 1337
PsiI TTATAA 2 cut(s) 1187, 1709
Psp1406I AACGTT 1 cut(s) 1053
Psp6I CCWGG 5 cut(s) 10, 491, 550, 1215, 1510
PspFI CCCAGC 4 cut(s) 639, 710, 787, 1747
PspGI CCWGG 5 cut(s) 10, 491, 550, 1215, 1510
PspN4I GGNNCC 7 cut(s) 16, 490, 598, 904, 1214, 1474, 2028
PspPI GGNCC 7 cut(s) 768, 857, 1034, 1085, 1199, 1508, 2026
PstI CTGCAG 1 cut(s) 997
PsuI RGATCY 4 cut(s) 14, 127, 1546, 1643
PsyI GACNNNGTC 1 cut(s) 1512
PvuI CGATCG 2 cut(s) 147, 345
RsaI GTAC 6 cut(s) 219, 340, 880, 891, 1335, 1855
RsaNI GTAC 6 cut(s) 218, 339, 879, 890, 1334, 1854
RseI CAYNNNNRTG 1 cut(s) 1356
SaqAI TTAA 8 cut(s) 53, 158, 588, 885, 944, 1241, 1464, 2034
SatI GCNGC 4 cut(s) 563, 693, 1324, 1893
Sau3AI GATC 8 cut(s) 14, 127, 144, 151, 342, 1546, 1643, 1870
Sau96I GGNCC 7 cut(s) 768, 857, 1034, 1085, 1199, 1508, 2026
SbfI CCTGCAGG 1 cut(s) 997
SchI GAGTC 2 cut(s) 220, 1421
ScrFI CCNGG 5 cut(s) 12, 493, 552, 1217, 1512
SdaI CCTGCAGG 1 cut(s) 997
SduI GDGCHC 3 cut(s) 756, 1306, 1363
SexAI ACCWGGT 1 cut(s) 1510
SfaNI GCATC 2 cut(s) 172, 486
SfcI CTRYAG 1 cut(s) 993
SinI GGWCC 5 cut(s) 1034, 1085, 1199, 1508, 2026
SmiMI CAYNNNNRTG 1 cut(s) 1356
SmlI CTYRAG 2 cut(s) 544, 1354
SmoI CTYRAG 2 cut(s) 544, 1354
SnaBI TACGTA 1 cut(s) 1337
Sse8387I CCTGCAGG 1 cut(s) 997
Sse9I AATT 8 cut(s) 1059, 1203, 1268, 1461, 1588, 1710, 1741, 2031
SspI AATATT 1 cut(s) 1078
SspMI CTAG 5 cut(s) 468, 719, 731, 843, 1941
StyD4I CCNGG 5 cut(s) 10, 491, 550, 1215, 1510
StyI CCWWGG 2 cut(s) 40, 860
TaaI ACNGT 2 cut(s) 50, 1616
TaiI ACGT 6 cut(s) 426, 527, 891, 1056, 1155, 1339
TaqI TCGA 4 cut(s) 143, 224, 1569, 1976
TaqII GACCGA 1 cut(s) 1073
TasI AATT 8 cut(s) 1059, 1203, 1268, 1461, 1588, 1710, 1741, 2031
TauI GCSGC 2 cut(s) 565, 695
TfiI GAWTC 4 cut(s) 386, 1623, 1960, 1982
Tru1I TTAA 8 cut(s) 53, 158, 588, 885, 944, 1241, 1464, 2034
Tru9I TTAA 8 cut(s) 53, 158, 588, 885, 944, 1241, 1464, 2034
TscAI CASTG 8 cut(s) 53, 409, 1531, 1770, 1795, 1836, 1936, 2001
TseFI GTSAC 3 cut(s) 269, 302, 520
TseI GCWGC 2 cut(s) 1323, 1892
Tsp45I GTSAC 3 cut(s) 269, 302, 520
TspDTI ATGAA 6 cut(s) 16, 171, 813, 1423, 1995, 2034
TspGWI ACGGA 3 cut(s) 131, 251, 1574
TspRI CASTG 8 cut(s) 53, 409, 1531, 1770, 1795, 1836, 1936, 2001
Tth111I GACNNNGTC 1 cut(s) 1512
Van91I CCANNNNNTGG 2 cut(s) 866, 1517
VneI GTGCAC 1 cut(s) 1359
VpaK11BI GGWCC 5 cut(s) 1034, 1085, 1199, 1508, 2026
XapI RAATTY 1 cut(s) 1059
XceI RCATGY 2 cut(s) 112, 1506
XmiI GTMKAC 2 cut(s) 793, 1578
XspI CTAG 5 cut(s) 468, 719, 731, 843, 1941
ZraI GACGTC 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.