MD09G1203400.v1.1

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000579
Physical Location & Seq
Forward (+)
19090463 .. 19096965
6503 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1203400.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGAGAAATGCACTTGTAATTGGCTTTCTAATACTTTGCATTGCTTCGTCCAATTTTAGTATTGGGCGTGGCCTGACAACTTTTAATGTGCTTGACTACGGTGCTCTTGGTGATGGCAAAACTGATGATTCGAAAGTAAGTTTTTCATCTGTCTACGCTCGTTTATCTTTCTTGAAAGCATGGAATGTTTCATGCGTACAAACGCAAGGGATATCATCAACACTTGAAATACCAGCGAATAAAACATTCCTTTTACAACCTACCAAATTCTCAGGCCCTTGCAAATCTAGCTCTGTCCATGTCCAGATTTTGGGAAAAATTGTGGCACCTAGCAATCTGGATGCATGGAAAGAGTGCGGCAGCGAATCATGGCTAACATTTTTAAAAGTGACCAACCTCATCATCAATGGAACCGGAGAAATCAATGGGCAGGGTTCGCCATGGTGGAGCAACGCTACCCTACTTAAATTACAATTTAAATGTGATGATTGTATTACAATTATTAATGGCTCGTCTTATATAAATATCACCAACATTGCATGCGGACCAGGTCATGGAATTAGGTTACGCTCCGATGACTCCAATTCTTACCGTTGTCGGACAAGAAGAAGTGGGTTAGATGCTGTTGATGGTGGTGCTACAAGCGGATGTTGTCGCTCAAAATGGTTAGGCGGTTATGGATATGCGAAGAATATATCTTTTAAGCAAATCAAACTAGTAGCAGCCAAAAATCCGATTATCATTGACCAACATTATTGTAATGGTGGACATGATTGCAAAAATTACACTTCGGCAGTTAGTGAGACTGATGTCTCATACAGTGATTTTCAAAGAACTTCTGAAACTGAAGAAGCCATTAAATTTGATTGCTCTGAGCCTCCAGGTTGTATTAATATTGTTATGGACCAAATTAACAATACTTCTGCAGTTGCTGGGAAGGAGGTTCATGCCTTTTGCATCAATGTGAATGGAACCTCCAATGCAACTGTTCCTGTTGTCCCTTGTCTGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

339

Amino Acids

36.48

Weight (kDa)

8.41

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 70 - 153 8.1e-12 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 162 - 323 1.9e-22 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 325
AccB7I CCANNNNNTGG 2 cut(s) 310, 554
AccI GTMKAC 1 cut(s) 153
AciI CCGC 4 cut(s) 357, 543, 645, 672
AcsI RAATTY 2 cut(s) 266, 860
AcuI CTGAAG 1 cut(s) 867
AfaI GTAC 1 cut(s) 198
AfiI CCNNNNNNNGG 2 cut(s) 310, 554
AgsI TTSAA 3 cut(s) 175, 227, 830
AhlI ACTAGT 1 cut(s) 715
AjnI CCWGG 2 cut(s) 547, 880
AluBI AGCT 1 cut(s) 291
AluI AGCT 1 cut(s) 291
Alw21I GWGCWC 1 cut(s) 106
Alw26I GTCTC 2 cut(s) 797, 817
AlwNI CAGNNNCTG 1 cut(s) 932
AoxI GGCC 2 cut(s) 70, 274
ApeKI GCWGC 2 cut(s) 360, 722
ApoI RAATTY 2 cut(s) 266, 860
AseI ATTAAT 2 cut(s) 504, 891
AspS9I GGNCC 3 cut(s) 275, 545, 904
AsuHPI GGTGA 2 cut(s) 122, 520
AsuII TTCGAA 1 cut(s) 131
AvaII GGWCC 2 cut(s) 545, 904
BanI GGYRCC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 372, 734
BccI CCATC 2 cut(s) 107, 623
BciT130I CCWGG 2 cut(s) 549, 882
BcoDI GTCTC 2 cut(s) 797, 817
BcuI ACTAGT 1 cut(s) 715
BfaI CTAG 3 cut(s) 288, 330, 716
BfmI CTRYAG 1 cut(s) 924
BisI GCNGC 3 cut(s) 358, 361, 723
BlsI GCNGC 3 cut(s) 359, 362, 724
Bme1390I CCNGG 2 cut(s) 549, 882
Bme18I GGWCC 2 cut(s) 545, 904
BmgT120I GGNCC 3 cut(s) 275, 545, 904
BmiI GGNNCC 3 cut(s) 327, 412, 973
BmrFI CCNGG 2 cut(s) 549, 882
BmsI GCATC 3 cut(s) 331, 610, 966
BoxI GACNNNNGTC 1 cut(s) 809
BpmI CTGGAG 1 cut(s) 864
Bpu14I TTCGAA 1 cut(s) 131
BsaJI CCNNGG 1 cut(s) 440
BsaWI WCCGGW 1 cut(s) 413
Bsc4I CCNNNNNNNGG 2 cut(s) 310, 554
Bse3DI GCAATG 2 cut(s) 39, 534
BseBI CCWGG 2 cut(s) 549, 882
BseDI CCNNGG 1 cut(s) 440
BseGI GGATG 2 cut(s) 346, 653
BseLI CCNNNNNNNGG 2 cut(s) 310, 554
BseMI GCAATG 2 cut(s) 39, 534
BseMII CTCAG 2 cut(s) 285, 864
BseXI GCAGC 2 cut(s) 372, 734
BseYI CCCAGC 1 cut(s) 932
BshFI GGCC 2 cut(s) 72, 276
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 106
BsiSI CCGG 1 cut(s) 414
BslFI GGGAC 1 cut(s) 983
BslI CCNNNNNNNGG 2 cut(s) 310, 554
BsmAI GTCTC 2 cut(s) 797, 817
BsmFI GGGAC 1 cut(s) 983
BsnI GGCC 2 cut(s) 72, 276
Bsp119I TTCGAA 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 106
Bsp19I CCATGG 1 cut(s) 440
BspACI CCGC 4 cut(s) 357, 543, 645, 672
BspANI GGCC 2 cut(s) 72, 276
BspCNI CTCAG 2 cut(s) 284, 865
BspLI GGNNCC 3 cut(s) 327, 412, 973
BspMAI CTGCAG 1 cut(s) 928
BspT104I TTCGAA 1 cut(s) 131
BspT107I GGYRCC 1 cut(s) 325
BsrDI GCAATG 2 cut(s) 39, 534
BssECI CCNNGG 1 cut(s) 440
BssT1I CCWWGG 1 cut(s) 440
Bst2UI CCWGG 2 cut(s) 549, 882
Bst4CI ACNGT 4 cut(s) 101, 593, 821, 988
BstBI TTCGAA 1 cut(s) 131
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 271, 873
BstDSI CCRYGG 1 cut(s) 440
BstF5I GGATG 2 cut(s) 346, 653
BstMAI GTCTC 2 cut(s) 797, 817
BstMWI GCNNNNNNNGC 2 cut(s) 288, 436
BstNI CCWGG 2 cut(s) 549, 882
BstNSI RCATGY 1 cut(s) 543
BstPAI GACNNNNGTC 1 cut(s) 809
BstSCI CCNGG 2 cut(s) 547, 880
BstSFI CTRYAG 1 cut(s) 924
BstV1I GCAGC 2 cut(s) 372, 734
BsuRI GGCC 2 cut(s) 72, 276
BtgI CCRYGG 1 cut(s) 440
BtsCI GGATG 2 cut(s) 346, 653
BtsIMutI CAGTG 1 cut(s) 826
Cac8I GCNNGC 1 cut(s) 541
CaiI CAGNNNCTG 1 cut(s) 932
Cfr13I GGNCC 3 cut(s) 275, 545, 904
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 1 cut(s) 197
CviJI RGCY 9 cut(s) 24, 72, 276, 291, 373, 510, 725, 854, 877
CviKI_1 RGCY 9 cut(s) 24, 72, 276, 291, 373, 510, 725, 854, 877
CviQI GTAC 1 cut(s) 197
DdeI CTNAG 2 cut(s) 271, 873
DraI TTTAAA 2 cut(s) 384, 478
Eco130I CCWWGG 1 cut(s) 440
Eco32I GATATC 1 cut(s) 213
Eco47I GGWCC 2 cut(s) 545, 904
Eco57I CTGAAG 1 cut(s) 867
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 547, 880
EcoRV GATATC 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 440
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 440
FaqI GGGAC 1 cut(s) 983
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 3 cut(s) 358, 361, 723
FokI GGATG 2 cut(s) 353, 660
Fsp4HI GCNGC 3 cut(s) 358, 361, 723
FspBI CTAG 3 cut(s) 288, 330, 716
GluI GCNGC 3 cut(s) 358, 361, 723
GsaI CCCAGC 1 cut(s) 936
GsuI CTGGAG 1 cut(s) 864
HaeIII GGCC 2 cut(s) 72, 276
HapII CCGG 1 cut(s) 414
HinfI GANTC 3 cut(s) 128, 365, 578
HpaII CCGG 1 cut(s) 414
HphI GGTGA 2 cut(s) 122, 520
Hpy166II GTNNAC 2 cut(s) 154, 767
Hpy188I TCNGA 6 cut(s) 574, 600, 735, 841, 874, 1008
Hpy188III TCNNGA 3 cut(s) 172, 304, 338
Hpy8I GTNNAC 2 cut(s) 154, 767
HpyAV CCTTC 1 cut(s) 931
HpyCH4III ACNGT 4 cut(s) 101, 593, 821, 988
HpyCH4V TGCA 9 cut(s) 11, 39, 282, 344, 539, 777, 926, 957, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 288, 436
HpyF3I CTNAG 2 cut(s) 271, 873
LmnI GCTCC 2 cut(s) 447, 575
Lsp1109I GCAGC 2 cut(s) 372, 734
LweI GCATC 3 cut(s) 331, 610, 966
MabI ACCWGGT 1 cut(s) 547
MaeI CTAG 3 cut(s) 288, 330, 716
MaeIII GTNAC 2 cut(s) 388, 564
MboII GAAGA 3 cut(s) 618, 700, 860
MhlI GDGCHC 1 cut(s) 106
MlyI GAGTC 1 cut(s) 572
MmeI TCCRAC 1 cut(s) 578
MnlI CCTC 4 cut(s) 407, 888, 934, 985
Mph1103I ATGCAT 1 cut(s) 346
MseI TTAA 9 cut(s) 84, 383, 465, 477, 504, 702, 858, 891, 912
MslI CAYNNNNRTG 1 cut(s) 962
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 2 cut(s) 549, 882
MvaI CCWGG 2 cut(s) 549, 882
MwoI GCNNNNNNNGC 2 cut(s) 288, 436
NcoI CCATGG 1 cut(s) 440
NlaIV GGNNCC 3 cut(s) 327, 412, 973
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 1 cut(s) 543
NspV TTCGAA 1 cut(s) 131
PaeI GCATGC 1 cut(s) 543
PfeI GAWTC 2 cut(s) 128, 365
PflFI GACNNNGTC 1 cut(s) 549
PflMI CCANNNNNTGG 2 cut(s) 310, 554
PkrI GCNGC 3 cut(s) 359, 362, 724
PleI GAGTC 1 cut(s) 572
PpsI GAGTC 1 cut(s) 572
PshAI GACNNNNGTC 1 cut(s) 809
PshBI ATTAAT 2 cut(s) 504, 891
Psp6I CCWGG 2 cut(s) 547, 880
PspFI CCCAGC 1 cut(s) 932
PspGI CCWGG 2 cut(s) 547, 880
PspN4I GGNNCC 3 cut(s) 327, 412, 973
PspPI GGNCC 3 cut(s) 275, 545, 904
PstI CTGCAG 1 cut(s) 928
PstNI CAGNNNCTG 1 cut(s) 932
PsyI GACNNNGTC 1 cut(s) 549
RsaI GTAC 1 cut(s) 198
RsaNI GTAC 1 cut(s) 197
RseI CAYNNNNRTG 1 cut(s) 962
SaqAI TTAA 9 cut(s) 84, 383, 465, 477, 504, 702, 858, 891, 912
SatI GCNGC 3 cut(s) 358, 361, 723
Sau96I GGNCC 3 cut(s) 275, 545, 904
SchI GAGTC 1 cut(s) 572
ScrFI CCNGG 2 cut(s) 549, 882
SduI GDGCHC 1 cut(s) 106
SetI ASST 9 cut(s) 262, 293, 331, 399, 553, 566, 886, 945, 977
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 3 cut(s) 331, 610, 966
SfcI CTRYAG 1 cut(s) 924
SfuI TTCGAA 1 cut(s) 131
SinI GGWCC 2 cut(s) 545, 904
SmiI ATTTAAAT 1 cut(s) 478
SmiMI CAYNNNNRTG 1 cut(s) 962
SpeI ACTAGT 1 cut(s) 715
SphI GCATGC 1 cut(s) 543
SsiI CCGC 4 cut(s) 357, 543, 645, 672
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 288, 330, 716
StyD4I CCNGG 2 cut(s) 547, 880
StyI CCWWGG 1 cut(s) 440
SwaI ATTTAAAT 1 cut(s) 478
TaaI ACNGT 4 cut(s) 101, 593, 821, 988
TaqI TCGA 1 cut(s) 131
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 2 cut(s) 128, 365
Tru1I TTAA 9 cut(s) 84, 383, 465, 477, 504, 702, 858, 891, 912
Tru9I TTAA 9 cut(s) 84, 383, 465, 477, 504, 702, 858, 891, 912
TscAI CASTG 1 cut(s) 826
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 360, 722
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 3 cut(s) 135, 180, 935
TspRI CASTG 1 cut(s) 826
Tth111I GACNNNGTC 1 cut(s) 549
Van91I CCANNNNNTGG 2 cut(s) 310, 554
VpaK11BI GGWCC 2 cut(s) 545, 904
VspI ATTAAT 2 cut(s) 504, 891
XapI RAATTY 2 cut(s) 266, 860
XceI RCATGY 1 cut(s) 543
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 3 cut(s) 288, 330, 716
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.