RchiOBHm_Chr2g0146281

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000579
Physical Location & Seq
Reverse (-)
63961647 .. 63963647
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51604

Sequence Viewer

Length: 834 bp
ATGACCCTGGCGGCCATCAAGATTTCGGGAAAAATTGTGGCACCCAGTTCAGAGGCCTGGAAAGAGTGTGCAAACCCTTGGCTAGCCTTCTTCCAGGTGACCAACCTCATCGTCAGTGGATCAGGAGAAATCAATGGCCAAGGTTCCTCTTGGTGGGCTAAATATGACCAACTTAACTATGCATTAGGTTTCCGCCGATGCGATAATCTTCAATTAAGTGGACTTACTCATGTTGACAGTCCAAAAACACATATTTCCATAAAATCTTGCAACAATGCAACTGTCTCTTATCTTACTATTAGGGCACCTGAAGATAGTCCGAACACAGATGGAATGGACATATCTATGTCAAGCCATGTCAATATTCATGATTGCAATATTGGAACCGGCCAAAATGGAGCTTATGAAACAGTAGAAGAGGTACATGTACAAAATTGCAGTTTCACTAGTACAATGTATGGAGCCAGAATCAAGACATATCAGGGTGGTTCCGGATATGCTAGAAAGATATCGTATGAACAAATCGCCCTTAGAACTACTAGGAACCCCATTATTATTGACCAATTCTACTGTAATGGTCAGCATGACTGCCAAACTTCGACTTCGGCCGTACGGGTGAGTGAAGTGACATACAATGGGTTTCATGGAACTTCTGAGAACGAGCAAGCAATTACATTTAATTGCAGCCAAAGTATAAGTTGTGCCAACATTGTGATGAATCAAGTGAGTATCACTTCACCAGTTCCCAGTGAGCAGGTTAGGGCTTCTTGCAGCAATGTAAATGGGACTTCTCACGCAACTGTACCACAAGTCCCTTGCCTAAAAAATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

277

Amino Acids

30.14

Weight (kDa)

6.22

Isoelectric Point (pI)

46.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 6 - 130 5.4e-25 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 133 - 263 1.1e-28 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 40, 304
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 2 cut(s) 11, 193
AclWI GGATC 1 cut(s) 127
AcoI YGGCCR 4 cut(s) 12, 136, 388, 606
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 5 cut(s) 423, 429, 451, 612, 804
AfiI CCNNNNNNNGG 1 cut(s) 153
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 1 cut(s) 212
AhlI ACTAGT 1 cut(s) 446
AjnI CCWGG 3 cut(s) 6, 56, 93
AluBI AGCT 1 cut(s) 401
AluI AGCT 1 cut(s) 401
Alw26I GTCTC 1 cut(s) 289
AlwI GGATC 1 cut(s) 127
Aor13HI TCCGGA 1 cut(s) 491
AoxI GGCC 5 cut(s) 12, 54, 136, 388, 606
ApeKI GCWGC 2 cut(s) 684, 771
AsuHPI GGTGA 3 cut(s) 109, 628, 729
AsuNHI GCTAGC 1 cut(s) 82
BaeGI GKGCMC 1 cut(s) 307
BalI TGGCCA 1 cut(s) 138
BanI GGYRCC 2 cut(s) 40, 304
BbvI GCAGC 2 cut(s) 696, 783
BccI CCATC 2 cut(s) 23, 323
BceAI ACGGC 1 cut(s) 593
BciT130I CCWGG 3 cut(s) 8, 58, 95
BcoDI GTCTC 1 cut(s) 289
BcuI ACTAGT 1 cut(s) 446
BfaI CTAG 4 cut(s) 83, 447, 501, 540
BfuAI ACCTGC 1 cut(s) 745
BisI GCNGC 3 cut(s) 12, 685, 772
BlsI GCNGC 3 cut(s) 13, 686, 773
Bme1390I CCNGG 3 cut(s) 8, 58, 95
BmiI GGNNCC 7 cut(s) 42, 145, 306, 385, 463, 490, 545
BmrFI CCNGG 3 cut(s) 8, 58, 95
BmrI ACTGGG 2 cut(s) 39, 741
BmsI GCATC 1 cut(s) 188
BmtI GCTAGC 1 cut(s) 86
BmuI ACTGGG 2 cut(s) 39, 741
BsaJI CCNNGG 3 cut(s) 6, 77, 139
BsaWI WCCGGW 1 cut(s) 491
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 386
Bse1I ACTGG 3 cut(s) 45, 740, 747
Bse3DI GCAATG 1 cut(s) 781
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 3 cut(s) 8, 58, 95
BseDI CCNNGG 3 cut(s) 6, 77, 139
BseLI CCNNNNNNNGG 1 cut(s) 153
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 1 cut(s) 645
BseNI ACTGG 3 cut(s) 45, 740, 747
BseSI GKGCMC 1 cut(s) 307
BseX3I CGGCCG 1 cut(s) 606
BseXI GCAGC 2 cut(s) 696, 783
Bsh1285I CGRYCG 1 cut(s) 609
BshFI GGCC 5 cut(s) 14, 56, 138, 390, 608
BshNI GGYRCC 2 cut(s) 40, 304
BsiEI CGRYCG 1 cut(s) 609
BsiSI CCGG 2 cut(s) 387, 492
BsiWI CGTACG 1 cut(s) 610
BslFI GGGAC 2 cut(s) 797, 799
BslI CCNNNNNNNGG 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 2 cut(s) 797, 799
BsnI GGCC 5 cut(s) 14, 56, 138, 390, 608
Bsp1286I GDGCHC 1 cut(s) 307
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 427
Bsp143I GATC 1 cut(s) 119
BspACI CCGC 2 cut(s) 11, 193
BspANI GGCC 5 cut(s) 14, 56, 138, 390, 608
BspCNI CTCAG 1 cut(s) 646
BspEI TCCGGA 1 cut(s) 491
BspHI TCATGA 1 cut(s) 367
BspLI GGNNCC 7 cut(s) 42, 145, 306, 385, 463, 490, 545
BspMI ACCTGC 1 cut(s) 745
BspOI GCTAGC 1 cut(s) 86
BspPI GGATC 1 cut(s) 127
BspT107I GGYRCC 2 cut(s) 40, 304
BsrDI GCAATG 1 cut(s) 781
BsrFI RCCGGY 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 427
BsrI ACTGG 3 cut(s) 45, 740, 747
BssAI RCCGGY 1 cut(s) 386
BssECI CCNNGG 3 cut(s) 6, 77, 139
BssMI GATC 1 cut(s) 119
BssT1I CCWWGG 2 cut(s) 77, 139
Bst2UI CCWGG 3 cut(s) 8, 58, 95
Bst4CI ACNGT 5 cut(s) 239, 283, 412, 572, 802
Bst6I CTCTTC 1 cut(s) 411
BstAUI TGTACA 1 cut(s) 427
BstC8I GCNNGC 2 cut(s) 84, 666
BstDEI CTNAG 2 cut(s) 530, 654
BstEII GGTNACC 1 cut(s) 97
BstKTI GATC 1 cut(s) 122
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 1 cut(s) 119
BstMCI CGRYCG 1 cut(s) 609
BstNI CCWGG 3 cut(s) 8, 58, 95
BstNSI RCATGY 1 cut(s) 428
BstPI GGTNACC 1 cut(s) 97
BstSCI CCNGG 3 cut(s) 6, 56, 93
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 2 cut(s) 696, 783
BstZI CGGCCG 1 cut(s) 606
BsuRI GGCC 5 cut(s) 14, 56, 138, 390, 608
BtsIMutI CAGTG 2 cut(s) 121, 754
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 2 cut(s) 84, 666
CciI TCATGA 1 cut(s) 367
Cfr10I RCCGGY 1 cut(s) 386
Csp6I GTAC 5 cut(s) 422, 428, 450, 611, 803
CviAII CATG 6 cut(s) 230, 356, 368, 425, 584, 644
CviQI GTAC 5 cut(s) 422, 428, 450, 611, 803
DdeI CTNAG 2 cut(s) 530, 654
DpnI GATC 1 cut(s) 121
DpnII GATC 1 cut(s) 119
EaeI YGGCCR 4 cut(s) 12, 136, 388, 606
EagI CGGCCG 1 cut(s) 606
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EciI GGCGGA 1 cut(s) 182
EclXI CGGCCG 1 cut(s) 606
Eco130I CCWWGG 2 cut(s) 77, 139
Eco147I AGGCCT 1 cut(s) 56
Eco32I GATATC 1 cut(s) 510
Eco52I CGGCCG 1 cut(s) 606
Eco57I CTGAAG 1 cut(s) 330
Eco91I GGTNACC 1 cut(s) 97
EcoO65I GGTNACC 1 cut(s) 97
EcoRII CCWGG 3 cut(s) 6, 56, 93
EcoRV GATATC 1 cut(s) 510
EcoT14I CCWWGG 2 cut(s) 77, 139
EcoT22I ATGCAT 1 cut(s) 184
ErhI CCWWGG 2 cut(s) 77, 139
FaeI CATG 6 cut(s) 233, 359, 371, 428, 587, 647
FaqI GGGAC 2 cut(s) 797, 799
FatI CATG 6 cut(s) 229, 355, 367, 424, 583, 643
Fnu4HI GCNGC 3 cut(s) 12, 685, 772
Fsp4HI GCNGC 3 cut(s) 12, 685, 772
FspBI CTAG 4 cut(s) 83, 447, 501, 540
GluI GCNGC 3 cut(s) 12, 685, 772
HaeIII GGCC 5 cut(s) 14, 56, 138, 390, 608
HapII CCGG 2 cut(s) 387, 492
Hin1II CATG 6 cut(s) 233, 359, 371, 428, 587, 647
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HinfI GANTC 2 cut(s) 468, 718
HpaII CCGG 2 cut(s) 387, 492
HphI GGTGA 3 cut(s) 109, 628, 729
Hpy166II GTNNAC 2 cut(s) 221, 235
Hpy188I TCNGA 3 cut(s) 52, 321, 655
Hpy188III TCNNGA 6 cut(s) 19, 27, 123, 368, 472, 492
Hpy8I GTNNAC 2 cut(s) 221, 235
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 5 cut(s) 239, 283, 412, 572, 802
HpyCH4V TGCA 8 cut(s) 71, 182, 270, 278, 375, 438, 684, 771
HpyF3I CTNAG 2 cut(s) 530, 654
Hsp92II CATG 6 cut(s) 233, 359, 371, 428, 587, 647
Kpn2I TCCGGA 1 cut(s) 491
Kzo9I GATC 1 cut(s) 119
LmnI GCTCC 2 cut(s) 398, 461
Lsp1109I GCAGC 2 cut(s) 696, 783
LweI GCATC 1 cut(s) 188
MaeI CTAG 4 cut(s) 83, 447, 501, 540
MaeIII GTNAC 2 cut(s) 97, 625
MalI GATC 1 cut(s) 121
MboI GATC 1 cut(s) 119
MboII GAAGA 4 cut(s) 82, 200, 323, 428
MhlI GDGCHC 1 cut(s) 307
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 6 cut(s) 33, 212, 433, 563, 669, 679
MluNI TGGCCA 1 cut(s) 138
MnlI CCTC 4 cut(s) 46, 116, 157, 412
Mox20I TGGCCA 1 cut(s) 138
Mph1103I ATGCAT 1 cut(s) 184
MroI TCCGGA 1 cut(s) 491
MscI TGGCCA 1 cut(s) 138
MseI TTAA 4 cut(s) 174, 215, 678, 832
MslI CAYNNNNRTG 2 cut(s) 344, 713
Msp20I TGGCCA 1 cut(s) 138
MspI CCGG 2 cut(s) 387, 492
MspR9I CCNGG 3 cut(s) 8, 58, 95
MvaI CCWGG 3 cut(s) 8, 58, 95
NdeII GATC 1 cut(s) 119
NheI GCTAGC 1 cut(s) 82
NlaIII CATG 6 cut(s) 233, 359, 371, 428, 587, 647
NlaIV GGNNCC 7 cut(s) 42, 145, 306, 385, 463, 490, 545
NmuCI GTSAC 2 cut(s) 97, 625
NsiI ATGCAT 1 cut(s) 184
NspI RCATGY 1 cut(s) 428
PagI TCATGA 1 cut(s) 367
PceI AGGCCT 1 cut(s) 56
PciI ACATGT 1 cut(s) 424
PfeI GAWTC 2 cut(s) 468, 718
Pfl23II CGTACG 1 cut(s) 610
PkrI GCNGC 3 cut(s) 13, 686, 773
PscI ACATGT 1 cut(s) 424
Psp6I CCWGG 3 cut(s) 6, 56, 93
PspEI GGTNACC 1 cut(s) 97
PspGI CCWGG 3 cut(s) 6, 56, 93
PspLI CGTACG 1 cut(s) 610
PspN4I GGNNCC 7 cut(s) 42, 145, 306, 385, 463, 490, 545
RsaI GTAC 5 cut(s) 423, 429, 451, 612, 804
RsaNI GTAC 5 cut(s) 422, 428, 450, 611, 803
RseI CAYNNNNRTG 2 cut(s) 344, 713
SaqAI TTAA 4 cut(s) 174, 215, 678, 832
SatI GCNGC 3 cut(s) 12, 685, 772
Sau3AI GATC 1 cut(s) 119
ScrFI CCNGG 3 cut(s) 8, 58, 95
SduI GDGCHC 1 cut(s) 307
SetI ASST 8 cut(s) 99, 108, 145, 190, 310, 403, 423, 759
SfaNI GCATC 1 cut(s) 188
SmiMI CAYNNNNRTG 2 cut(s) 344, 713
SpeI ACTAGT 1 cut(s) 446
Sse9I AATT 6 cut(s) 33, 212, 433, 563, 669, 679
SseBI AGGCCT 1 cut(s) 56
SsiI CCGC 2 cut(s) 11, 193
SspI AATATT 3 cut(s) 364, 379, 829
SspMI CTAG 4 cut(s) 83, 447, 501, 540
StuI AGGCCT 1 cut(s) 56
StyD4I CCNGG 3 cut(s) 6, 56, 93
StyI CCWWGG 2 cut(s) 77, 139
TaaI ACNGT 5 cut(s) 239, 283, 412, 572, 802
TaqI TCGA 1 cut(s) 599
TasI AATT 6 cut(s) 33, 212, 433, 563, 669, 679
TatI WGTACW 2 cut(s) 427, 449
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 2 cut(s) 468, 718
Tru1I TTAA 4 cut(s) 174, 215, 678, 832
Tru9I TTAA 4 cut(s) 174, 215, 678, 832
TscAI CASTG 2 cut(s) 121, 754
TseFI GTSAC 2 cut(s) 97, 625
TseI GCWGC 2 cut(s) 684, 771
Tsp45I GTSAC 2 cut(s) 97, 625
TspDTI ATGAA 5 cut(s) 356, 420, 531, 632, 731
TspRI CASTG 2 cut(s) 121, 754
XceI RCATGY 1 cut(s) 428
XspI CTAG 4 cut(s) 83, 447, 501, 540
Zsp2I ATGCAT 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.