Rw0G012400

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00549
OG0000579
Physical Location & Seq
Forward (+)
203519 .. 205542
2024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G012400.1

Sequence Viewer

Length: 1230 bp
ATGCAGTACATTCTTGTGAGCTTTTTGATATTTTGCATATCTTCATCTCATTTAAGCGACATTGTATATGGAAAAAAAACATATGATGTTCGTAGTTATGGTGCTGTTGGAGATGGAAACGCTGATGATTCAAAATTAAGTTTTCTTTCTTCCTTTGGTTTTCCCATTCATATGTTTGCTTTTGTAAAAGCGTGGGAAGATTTATGTGGAGCATCTGAAACTTATGATGTACCAACACTTCAGATACCGGCGGGCAAAACATTCTTATTGCAGCCTACGAAATTTAAAGGTCCATGCAAATCAAAAAGCGTTCATGTTCAGGTGGTGGCACCCAAAACACCGGATGCTTGGAAACAGTGTGAATCAAATTACTGGCTTTCCTTTTCAAATGTGGCAAACCTCAGAATGAATGGCGGTTCAGGAATAATCGACGGCCAGGATGACAAGAAGAAGTGTCAACGACCAAAGGCTCTACACTTCCATGGATGTCATAATCTTCTACTAACTGGACTTACTCATGTAAATAGTCCAAAAGGTCATATTAGTATAAGTAACTGCAGCCATGTATATGTCGCCAATCTCACTATAACTGCACCTGAAGAGAGTCCAAACACCGATGGAATTGACATCTCAAACTCAAATCATGTCAACATTCATGCCTCTTATATTGGAACCGGTGAGGATTGTATAGCTATCAATTCTGGATGTTCTAATATCAACATTACCAACATTGCATGTGGCCCAGGCCATGGAATTAGCGTGGGAAGCTTGGGAGAAAATGGGGCTTACGCAACAGTAGAAAATGTATCTGTGAAAAATTGTAATTTCAGTAGAACTCAAAATGGAGTAAGAATCAAGACATGGCAGGGTGGATCTGGGTACGCCAAGAATATAACCTTTGAACAAATCACACTTCATGCCACTAAAAACCCAATTATCATCGACCAATATTATTGTAATGGAGCTCACAATTGTAAAAACAAGACCTCAGCTGTCAAGGTGAGTGATGTGAGATACAGGAATATCCAAGGTACTTCAGCAAAAAAGGAGGCGATAAGATTAGACTGCAATCAGTTTTCGGGTTGTCGCAACATTGTAATGGAGCACATCAACATCACCTCGAATGGTCCTGGTAATAAGGGGATACAGGCATCATGCTACAACATCAATGGAATTTCTAAAAGCACAGTGCCGAGAGTACTTAACCTGCAGTCCAAACTGGTTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

409

Amino Acids

44.38

Weight (kDa)

8.82

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 79 - 390 8.5e-86 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1215
AccB1I GGYRCC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 749
AciI CCGC 2 cut(s) 251, 414
AclWI GGATC 1 cut(s) 880
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 2 cut(s) 281, 1173
AcuI CTGAAG 3 cut(s) 224, 618, 1020
AfaI GTAC 5 cut(s) 8, 231, 881, 1033, 1200
AfiI CCNNNNNNNGG 1 cut(s) 749
AgeI ACCGGT 1 cut(s) 674
AgsI TTSAA 3 cut(s) 132, 387, 902
AjnI CCWGG 3 cut(s) 435, 742, 1129
AluBI AGCT 5 cut(s) 21, 692, 768, 965, 992
AluI AGCT 5 cut(s) 21, 692, 768, 965, 992
Alw21I GWGCWC 2 cut(s) 967, 1107
AlwI GGATC 1 cut(s) 880
AoxI GGCC 3 cut(s) 433, 739, 745
ApeKI GCWGC 2 cut(s) 271, 558
ApoI RAATTY 2 cut(s) 281, 1173
AsiGI ACCGGT 1 cut(s) 674
AspS9I GGNCC 3 cut(s) 290, 740, 1127
AsuHPI GGTGA 3 cut(s) 689, 1012, 1108
AvaII GGWCC 2 cut(s) 290, 1127
BanI GGYRCC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 967
BarI GAAGNNNNNNTAC 2 cut(s) 222, 254
Bbv12I GWGCWC 2 cut(s) 967, 1107
BbvCI CCTCAGC 1 cut(s) 988
BbvI GCAGC 2 cut(s) 283, 570
BccI CCATC 2 cut(s) 107, 611
BceAI ACGGC 1 cut(s) 448
BciT130I CCWGG 3 cut(s) 437, 744, 1131
BciVI GTATCC 1 cut(s) 1137
BfmI CTRYAG 2 cut(s) 556, 1208
BfuAI ACCTGC 1 cut(s) 1215
BfuI GTATCC 1 cut(s) 1137
BisI GCNGC 2 cut(s) 272, 559
BlsI GCNGC 2 cut(s) 273, 560
BmcAI AGTACT 1 cut(s) 1200
Bme1390I CCNGG 3 cut(s) 437, 744, 1131
Bme18I GGWCC 2 cut(s) 290, 1127
BmgT120I GGNCC 3 cut(s) 290, 740, 1127
BmiI GGNNCC 2 cut(s) 330, 673
BmrFI CCNGG 3 cut(s) 437, 744, 1131
BmsI GCATC 3 cut(s) 221, 334, 1160
Bpu10I CCTNAGC 1 cut(s) 988
BsaJI CCNNGG 4 cut(s) 481, 742, 748, 1027
BsaWI WCCGGW 2 cut(s) 340, 674
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse118I RCCGGY 2 cut(s) 247, 674
Bse1I ACTGG 3 cut(s) 377, 511, 1224
Bse3DI GCAATG 1 cut(s) 729
BseBI CCWGG 3 cut(s) 437, 744, 1131
BseDI CCNNGG 4 cut(s) 481, 742, 748, 1027
BseGI GGATG 4 cut(s) 349, 445, 491, 710
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 1 cut(s) 729
BseMII CTCAG 2 cut(s) 415, 1002
BseNI ACTGG 3 cut(s) 377, 511, 1224
BseXI GCAGC 2 cut(s) 283, 570
BsgI GTGCAG 1 cut(s) 576
BshFI GGCC 3 cut(s) 435, 741, 747
BshNI GGYRCC 1 cut(s) 328
BshTI ACCGGT 1 cut(s) 674
BsiHKAI GWGCWC 2 cut(s) 967, 1107
BsiSI CCGG 3 cut(s) 248, 341, 675
BslI CCNNNNNNNGG 1 cut(s) 749
BsnI GGCC 3 cut(s) 435, 741, 747
Bsp1286I GDGCHC 2 cut(s) 967, 1107
Bsp143I GATC 1 cut(s) 872
Bsp19I CCATGG 2 cut(s) 481, 748
BspACI CCGC 2 cut(s) 251, 414
BspANI GGCC 3 cut(s) 435, 741, 747
BspCNI CTCAG 2 cut(s) 414, 1001
BspLI GGNNCC 2 cut(s) 330, 673
BspMAI CTGCAG 2 cut(s) 560, 1212
BspMI ACCTGC 1 cut(s) 1215
BspPI GGATC 1 cut(s) 880
BspT107I GGYRCC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 729
BsrFI RCCGGY 2 cut(s) 247, 674
BsrI ACTGG 3 cut(s) 377, 511, 1224
BssAI RCCGGY 2 cut(s) 247, 674
BssECI CCNNGG 4 cut(s) 481, 742, 748, 1027
BssMI GATC 1 cut(s) 872
BssT1I CCWWGG 3 cut(s) 481, 748, 1027
Bst2UI CCWGG 3 cut(s) 437, 744, 1131
Bst4CI ACNGT 3 cut(s) 357, 796, 1189
Bst6I CTCTTC 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 2 cut(s) 401, 988
BstDSI CCRYGG 2 cut(s) 481, 748
BstF5I GGATG 4 cut(s) 349, 445, 491, 710
BstKTI GATC 1 cut(s) 875
BstMBI GATC 1 cut(s) 872
BstMWI GCNNNNNNNGC 1 cut(s) 765
BstNI CCWGG 3 cut(s) 437, 744, 1131
BstNSI RCATGY 1 cut(s) 738
BstSCI CCNGG 3 cut(s) 435, 742, 1129
BstSFI CTRYAG 2 cut(s) 556, 1208
BstV1I GCAGC 2 cut(s) 283, 570
BstX2I RGATCY 1 cut(s) 872
BstYI RGATCY 1 cut(s) 872
BsuI GTATCC 1 cut(s) 1137
BsuRI GGCC 3 cut(s) 435, 741, 747
BtgI CCRYGG 2 cut(s) 481, 748
BtsCI GGATG 4 cut(s) 349, 445, 491, 710
BtsIMutI CAGTG 2 cut(s) 362, 1194
BveI ACCTGC 1 cut(s) 1215
Cac8I GCNNGC 1 cut(s) 253
Cfr10I RCCGGY 2 cut(s) 247, 674
Cfr13I GGNCC 3 cut(s) 290, 740, 1127
Csp6I GTAC 5 cut(s) 7, 230, 880, 1032, 1199
CspAI ACCGGT 1 cut(s) 674
CviQI GTAC 5 cut(s) 7, 230, 880, 1032, 1199
DdeI CTNAG 2 cut(s) 401, 988
DpnI GATC 1 cut(s) 874
DpnII GATC 1 cut(s) 872
DraI TTTAAA 1 cut(s) 286
EaeI YGGCCR 1 cut(s) 433
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Ecl136II GAGCTC 1 cut(s) 965
Eco130I CCWWGG 3 cut(s) 481, 748, 1027
Eco24I GRGCYC 1 cut(s) 967
Eco47I GGWCC 2 cut(s) 290, 1127
Eco53kI GAGCTC 1 cut(s) 965
Eco57I CTGAAG 3 cut(s) 224, 618, 1020
EcoICRI GAGCTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 435, 742, 1129
EcoT14I CCWWGG 3 cut(s) 481, 748, 1027
EcoT38I GRGCYC 1 cut(s) 967
ErhI CCWWGG 3 cut(s) 481, 748, 1027
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 2 cut(s) 82, 171
Fnu4HI GCNGC 2 cut(s) 272, 559
FokI GGATG 4 cut(s) 356, 452, 498, 717
FriOI GRGCYC 1 cut(s) 967
Fsp4HI GCNGC 2 cut(s) 272, 559
GluI GCNGC 2 cut(s) 272, 559
HaeIII GGCC 3 cut(s) 435, 741, 747
HapII CCGG 3 cut(s) 248, 341, 675
HincII GTYRAC 2 cut(s) 458, 649
HindII GTYRAC 2 cut(s) 458, 649
HindIII AAGCTT 1 cut(s) 766
HinfI GANTC 4 cut(s) 128, 362, 604, 852
HpaII CCGG 3 cut(s) 248, 341, 675
HphI GGTGA 3 cut(s) 689, 1012, 1108
Hpy166II GTNNAC 2 cut(s) 458, 649
Hpy188I TCNGA 3 cut(s) 217, 243, 404
Hpy188III TCNNGA 3 cut(s) 420, 702, 856
Hpy8I GTNNAC 2 cut(s) 458, 649
Hpy99I CGWCG 1 cut(s) 434
HpyCH4III ACNGT 3 cut(s) 357, 796, 1189
HpyCH4V TGCA 9 cut(s) 4, 36, 271, 297, 558, 593, 734, 1068, 1210
HpyF10VI GCNNNNNNNGC 1 cut(s) 765
HpyF3I CTNAG 2 cut(s) 401, 988
Kzo9I GATC 1 cut(s) 872
LmnI GCTCC 3 cut(s) 209, 962, 1102
Lsp1109I GCAGC 2 cut(s) 283, 570
LweI GCATC 3 cut(s) 221, 334, 1160
MaeIII GTNAC 1 cut(s) 551
MalI GATC 1 cut(s) 874
MboI GATC 1 cut(s) 872
MboII GAAGA 6 cut(s) 33, 141, 209, 460, 488, 611
MfeI CAATTG 1 cut(s) 970
MflI RGATCY 1 cut(s) 872
MhlI GDGCHC 2 cut(s) 967, 1107
MlyI GAGTC 1 cut(s) 613
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 6 cut(s) 410, 670, 673, 997, 1042, 1129
MseI TTAA 4 cut(s) 53, 137, 285, 1203
MslI CAYNNNNRTG 5 cut(s) 14, 170, 480, 567, 1097
MspA1I CMGCKG 1 cut(s) 992
MspI CCGG 3 cut(s) 248, 341, 675
MspR9I CCNGG 3 cut(s) 437, 744, 1131
MunI CAATTG 1 cut(s) 970
MvaI CCWGG 3 cut(s) 437, 744, 1131
MwoI GCNNNNNNNGC 1 cut(s) 765
NcoI CCATGG 2 cut(s) 481, 748
NdeI CATATG 2 cut(s) 82, 171
NdeII GATC 1 cut(s) 872
NlaIV GGNNCC 2 cut(s) 330, 673
NmeAIII GCCGAG 1 cut(s) 1218
NspI RCATGY 1 cut(s) 738
PfeI GAWTC 3 cut(s) 128, 362, 852
PflMI CCANNNNNTGG 1 cut(s) 749
PinAI ACCGGT 1 cut(s) 674
PkrI GCNGC 2 cut(s) 273, 560
PleI GAGTC 1 cut(s) 612
PpsI GAGTC 1 cut(s) 612
Psp124BI GAGCTC 1 cut(s) 967
Psp6I CCWGG 3 cut(s) 435, 742, 1129
PspGI CCWGG 3 cut(s) 435, 742, 1129
PspN4I GGNNCC 2 cut(s) 330, 673
PspPI GGNCC 3 cut(s) 290, 740, 1127
PstI CTGCAG 2 cut(s) 560, 1212
PsuI RGATCY 1 cut(s) 872
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 5 cut(s) 8, 231, 881, 1033, 1200
RsaNI GTAC 5 cut(s) 7, 230, 880, 1032, 1199
RseI CAYNNNNRTG 5 cut(s) 14, 170, 480, 567, 1097
SacI GAGCTC 1 cut(s) 967
SaqAI TTAA 4 cut(s) 53, 137, 285, 1203
SatI GCNGC 2 cut(s) 272, 559
Sau3AI GATC 1 cut(s) 872
Sau96I GGNCC 3 cut(s) 290, 740, 1127
ScaI AGTACT 1 cut(s) 1200
SchI GAGTC 1 cut(s) 613
ScrFI CCNGG 3 cut(s) 437, 744, 1131
SduI GDGCHC 2 cut(s) 967, 1107
SfaNI GCATC 3 cut(s) 221, 334, 1160
SfcI CTRYAG 2 cut(s) 556, 1208
SinI GGWCC 2 cut(s) 290, 1127
SmiMI CAYNNNNRTG 5 cut(s) 14, 170, 480, 567, 1097
SsiI CCGC 2 cut(s) 251, 414
SspI AATATT 1 cut(s) 950
SstI GAGCTC 1 cut(s) 967
StyD4I CCNGG 3 cut(s) 435, 742, 1129
StyI CCWWGG 3 cut(s) 481, 748, 1027
TaaI ACNGT 3 cut(s) 357, 796, 1189
TaqI TCGA 3 cut(s) 429, 942, 1121
TatI WGTACW 2 cut(s) 6, 1198
TfiI GAWTC 3 cut(s) 128, 362, 852
Tru1I TTAA 4 cut(s) 53, 137, 285, 1203
Tru9I TTAA 4 cut(s) 53, 137, 285, 1203
TscAI CASTG 2 cut(s) 362, 1194
TseI GCWGC 2 cut(s) 271, 558
TspDTI ATGAA 6 cut(s) 33, 158, 302, 422, 644, 905
TspRI CASTG 2 cut(s) 362, 1194
Van91I CCANNNNNTGG 1 cut(s) 749
VpaK11BI GGWCC 2 cut(s) 290, 1127
XapI RAATTY 2 cut(s) 281, 1173
XceI RCATGY 1 cut(s) 738
ZrmI AGTACT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.