FvH4_6g32320

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000579
Physical Location & Seq
Reverse (-)
25369645 .. 25372747
3103 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32320.t1

Sequence Viewer

Length: 1287 bp
ATGGAAGCGGTGGAGGCAGACATGGAGACTGCCATGAGAGATCGCGCCGCACAGCTAAAAAACCCTAACCCTAGAGAGAGAGAGAGCGAAGAGAGGCTGCGTTTTGGACATGGGCAAACAACCTTTAATGTGCTTGACTATGGTGCTGCTGGAGATGGCGAGAGAGATGATTCTAAAGCATTCTTAGAAGCATGGAATGCATCATGTTCAACTTACGCTAGTGAAGGGACAACAACACTAGTAATACCAGCAGAAAAAACATTCCTTTTGCAACCTACCACCTTCTCAGGTTCTTGCAATTCTGGCAACGGTATCCGGGTCGAGATTATGGGGAAAATTGTGGCACCGAAAAATCCAGATGAATGGAAAGAGTGTGTAGAATCGTGGTTATCTTTTGGAAATCTGAGCAACCTCATCATAAACGGAACAGGAGAAATTAATGGCCAAGGTTCACCTTGGTGGAGCAATATTACCACCCAAGCGAGCAAGGTCAAATGCGAACGACCAAAGGCTCTACACTTCTTCAATTGCAGTAATCTTCGGTTAAGTGGAGTTACACATGTTGACAGTCCAAAGGCTCATATTAGCATCAATCGTAGTAATAATGTCACCGTCTCCGATATTCATATTCTTGCACCTCATGATAGTCCCAACACAGATGGTATTGACATCTCTGTCTCAACATATGTCAATATTCATGACTCCAAAATAGCAACTGGTGATGACTGTATTGCCATTAACAATGGTTCATCCTTCATCAATATCACCAATATCGCATGTGGACCTGGTCATGGAATTAGTGTGGGAAGTTTAGGGGTGAATAGAAGTTATCAGACAGCAGAACAAATAAACGTACGAAATTGTAGTTTCAACGGAACACAAAATGGAGCTAGACTTAAGACATGGCAGGGTGGCTCTGGGTATGCTAGGAAGATAACATTCGAAAATATAACACTAACAGATGCGAAGCACCCCATTATCATTGACCAACATTATTGTAATGGAAAACACGACTGTCCAAACTCGACTCAGGCAGTACAAGTGAGTGATGTCACATATAGTAATATTCAAGGAACTTCTTCAAGTATGGAGGCCATAGTATTTAACTGCACACAGATTCCCGGTTGTACTAACATTGTGATGAAACAAATCAATATCACTTCGGCAATTGCTTATCAGAACATTTCTGCCTCTTGTATCAATGCGAATGGGACTTGCAGCACAACGGTTCCCCAAGTCTCTTGCTTGAAGACAAATGAAACAATGTCTTTCAATCTATACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

429

Amino Acids

46.32

Weight (kDa)

5.78

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 77 - 407 4e-88 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 343
AccII CGCG 1 cut(s) 45
AciI CCGC 2 cut(s) 8, 48
AcoI YGGCCR 1 cut(s) 442
AfaI GTAC 3 cut(s) 855, 1038, 1129
AfiI CCNNNNNNNGG 1 cut(s) 791
AflII CTTAAG 1 cut(s) 896
AflIII ACRYGT 1 cut(s) 559
AgsI TTSAA 7 cut(s) 210, 526, 871, 1070, 1083, 1249, 1273
AhlI ACTAGT 1 cut(s) 238
AjnI CCWGG 1 cut(s) 784
AleI CACNNNNGTG 1 cut(s) 457
AluBI AGCT 2 cut(s) 55, 890
AluI AGCT 2 cut(s) 55, 890
Alw26I GTCTC 4 cut(s) 20, 619, 682, 1243
AoxI GGCC 2 cut(s) 442, 1092
ApeKI GCWGC 3 cut(s) 97, 146, 1218
AseI ATTAAT 1 cut(s) 438
Asp700I GAANNNNTTC 1 cut(s) 1078
AspLEI GCGC 1 cut(s) 47
AspS9I GGNCC 1 cut(s) 782
AsuC2I CCSGG 2 cut(s) 317, 1122
AsuHPI GGTGA 5 cut(s) 444, 601, 731, 757, 829
AsuII TTCGAA 1 cut(s) 942
AvaII GGWCC 1 cut(s) 782
BalI TGGCCA 1 cut(s) 444
BanI GGYRCC 1 cut(s) 343
BbsI GAAGAC 1 cut(s) 1256
BbvI GCAGC 3 cut(s) 84, 133, 1230
BccI CCATC 2 cut(s) 149, 653
BciT130I CCWGG 1 cut(s) 786
BciVI GTATCC 1 cut(s) 323
BcnI CCSGG 2 cut(s) 317, 1122
BcoDI GTCTC 4 cut(s) 20, 619, 682, 1243
BcuI ACTAGT 1 cut(s) 238
BfaI CTAG 5 cut(s) 72, 219, 239, 891, 927
BfrI CTTAAG 1 cut(s) 896
BfuI GTATCC 1 cut(s) 323
BisI GCNGC 4 cut(s) 48, 98, 147, 1219
BlsI GCNGC 4 cut(s) 49, 99, 148, 1220
Bme1390I CCNGG 3 cut(s) 317, 786, 1122
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 2 cut(s) 345, 1230
BmrFI CCNGG 3 cut(s) 317, 786, 1122
BmsI GCATC 3 cut(s) 209, 597, 952
BpiI GAAGAC 1 cut(s) 1256
BpmI CTGGAG 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 942
BpuMI CCSGG 2 cut(s) 317, 1122
BsaJI CCNNGG 2 cut(s) 445, 455
Bsc4I CCNNNNNNNGG 1 cut(s) 791
Bse1I ACTGG 1 cut(s) 721
BseBI CCWGG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 445, 455
BseGI GGATG 1 cut(s) 749
BseLI CCNNNNNNNGG 1 cut(s) 791
BseMII CTCAG 3 cut(s) 300, 395, 1043
BseNI ACTGG 1 cut(s) 721
BseXI GCAGC 3 cut(s) 84, 133, 1230
BsgI GTGCAG 1 cut(s) 1093
Bsh1236I CGCG 1 cut(s) 45
BshFI GGCC 2 cut(s) 444, 1094
BshNI GGYRCC 1 cut(s) 343
BsiSI CCGG 2 cut(s) 316, 1122
BsiWI CGTACG 1 cut(s) 853
BslFI GGGAC 3 cut(s) 241, 633, 1225
BslI CCNNNNNNNGG 1 cut(s) 791
BsmAI GTCTC 4 cut(s) 20, 619, 682, 1243
BsmBI CGTCTC 1 cut(s) 619
BsmFI GGGAC 3 cut(s) 241, 633, 1225
BsmI GAATGC 2 cut(s) 179, 202
BsnI GGCC 2 cut(s) 444, 1094
Bsp119I TTCGAA 1 cut(s) 942
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 2 cut(s) 8, 48
BspANI GGCC 2 cut(s) 444, 1094
BspCNI CTCAG 3 cut(s) 299, 396, 1042
BspFNI CGCG 1 cut(s) 45
BspHI TCATGA 2 cut(s) 640, 697
BspLI GGNNCC 2 cut(s) 345, 1230
BspT104I TTCGAA 1 cut(s) 942
BspT107I GGYRCC 1 cut(s) 343
BspTI CTTAAG 1 cut(s) 896
BsrI ACTGG 1 cut(s) 721
BssECI CCNNGG 2 cut(s) 445, 455
BssMI GATC 1 cut(s) 40
BssT1I CCWWGG 2 cut(s) 445, 455
Bst2UI CCWGG 1 cut(s) 786
Bst4CI ACNGT 6 cut(s) 311, 569, 613, 728, 1016, 1228
Bst6I CTCTTC 1 cut(s) 84
BstAFI CTTAAG 1 cut(s) 896
BstAPI GCANNNNNTGC 1 cut(s) 197
BstBI TTCGAA 1 cut(s) 942
BstC8I GCNNGC 1 cut(s) 484
BstDEI CTNAG 4 cut(s) 184, 286, 404, 1029
BstF5I GGATG 1 cut(s) 749
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 47
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 4 cut(s) 20, 619, 682, 1243
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 3 cut(s) 14, 197, 303
BstNI CCWGG 1 cut(s) 786
BstNSI RCATGY 2 cut(s) 563, 780
BstSCI CCNGG 3 cut(s) 315, 784, 1120
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 84, 133, 1230
BstV2I GAAGAC 1 cut(s) 1256
BstXI CCANNNNNNTGG 1 cut(s) 363
BsuI GTATCC 1 cut(s) 323
BsuRI GGCC 2 cut(s) 444, 1094
BtsCI GGATG 1 cut(s) 749
Cac8I GCNNGC 1 cut(s) 484
CciI TCATGA 2 cut(s) 640, 697
CfoI GCGC 1 cut(s) 47
Cfr13I GGNCC 1 cut(s) 782
CsiI ACCWGGT 1 cut(s) 784
Csp6I GTAC 3 cut(s) 854, 1037, 1128
CviJI RGCY 8 cut(s) 55, 97, 444, 512, 578, 890, 915, 1094
CviKI_1 RGCY 8 cut(s) 55, 97, 444, 512, 578, 890, 915, 1094
CviQI GTAC 3 cut(s) 854, 1037, 1128
DdeI CTNAG 4 cut(s) 184, 286, 404, 1029
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
DrdI GACNNNNNNGTC 1 cut(s) 674
DseDI GACNNNNNNGTC 1 cut(s) 674
EaeI YGGCCR 1 cut(s) 442
Eam1104I CTCTTC 1 cut(s) 84
EarI CTCTTC 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 445, 455
Eco47I GGWCC 1 cut(s) 782
EcoRII CCWGG 1 cut(s) 784
EcoT14I CCWWGG 2 cut(s) 445, 455
EcoT22I ATGCAT 1 cut(s) 202
ErhI CCWWGG 2 cut(s) 445, 455
Esp3I CGTCTC 1 cut(s) 619
FaqI GGGAC 3 cut(s) 241, 633, 1225
FauNDI CATATG 1 cut(s) 685
Fnu4HI GCNGC 4 cut(s) 48, 98, 147, 1219
FokI GGATG 1 cut(s) 736
Fsp4HI GCNGC 4 cut(s) 48, 98, 147, 1219
FspBI CTAG 5 cut(s) 72, 219, 239, 891, 927
GlaI GCGC 1 cut(s) 46
GluI GCNGC 4 cut(s) 48, 98, 147, 1219
GsuI CTGGAG 1 cut(s) 171
HaeIII GGCC 2 cut(s) 444, 1094
HapII CCGG 2 cut(s) 316, 1122
HhaI GCGC 1 cut(s) 47
Hin6I GCGC 1 cut(s) 45
HinP1I GCGC 1 cut(s) 45
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HinfI GANTC 5 cut(s) 170, 380, 701, 1027, 1117
HpaII CCGG 2 cut(s) 316, 1122
HphI GGTGA 5 cut(s) 444, 601, 731, 757, 829
Hpy166II GTNNAC 3 cut(s) 452, 565, 782
Hpy188I TCNGA 4 cut(s) 405, 619, 834, 1179
Hpy188III TCNNGA 4 cut(s) 322, 356, 641, 698
Hpy8I GTNNAC 3 cut(s) 452, 565, 782
HpyAV CCTTC 3 cut(s) 218, 292, 763
HpyCH4III ACNGT 6 cut(s) 311, 569, 613, 728, 1016, 1228
HpyCH4IV ACGT 1 cut(s) 852
HpyCH4V TGCA 7 cut(s) 200, 271, 297, 531, 635, 1110, 1218
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 197, 303
HpyF3I CTNAG 4 cut(s) 184, 286, 404, 1029
HpySE526I ACGT 1 cut(s) 852
HspAI GCGC 1 cut(s) 45
Kzo9I GATC 1 cut(s) 40
LmnI GCTCC 2 cut(s) 462, 887
Lsp1109I GCAGC 3 cut(s) 84, 133, 1230
LweI GCATC 3 cut(s) 209, 597, 952
MabI ACCWGGT 1 cut(s) 784
MaeI CTAG 5 cut(s) 72, 219, 239, 891, 927
MaeII ACGT 1 cut(s) 852
MaeIII GTNAC 3 cut(s) 553, 607, 1051
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 6 cut(s) 101, 514, 530, 943, 1071, 1261
MfeI CAATTG 2 cut(s) 526, 1167
MlsI TGGCCA 1 cut(s) 444
MluCI AATT 7 cut(s) 298, 336, 435, 526, 795, 859, 1167
MluNI TGGCCA 1 cut(s) 444
MlyI GAGTC 2 cut(s) 695, 1021
MnlI CCTC 6 cut(s) 7, 87, 422, 648, 1084, 1201
Mox20I TGGCCA 1 cut(s) 444
Mph1103I ATGCAT 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 1078
MscI TGGCCA 1 cut(s) 444
MseI TTAA 6 cut(s) 126, 438, 545, 738, 897, 1104
MslI CAYNNNNRTG 2 cut(s) 457, 1139
Msp20I TGGCCA 1 cut(s) 444
MspCI CTTAAG 1 cut(s) 896
MspI CCGG 2 cut(s) 316, 1122
MspR9I CCNGG 3 cut(s) 317, 786, 1122
MunI CAATTG 2 cut(s) 526, 1167
Mva1269I GAATGC 2 cut(s) 179, 202
MvaI CCWGG 1 cut(s) 786
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 3 cut(s) 14, 197, 303
NciI CCSGG 2 cut(s) 317, 1122
NdeI CATATG 1 cut(s) 685
NdeII GATC 1 cut(s) 40
NlaIV GGNNCC 2 cut(s) 345, 1230
NmuCI GTSAC 2 cut(s) 607, 1051
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 2 cut(s) 563, 780
NspV TTCGAA 1 cut(s) 942
OliI CACNNNNGTG 1 cut(s) 457
PagI TCATGA 2 cut(s) 640, 697
PciI ACATGT 1 cut(s) 559
PctI GAATGC 2 cut(s) 179, 202
PdmI GAANNNNTTC 1 cut(s) 1078
PfeI GAWTC 3 cut(s) 170, 380, 1117
Pfl23II CGTACG 1 cut(s) 853
PflFI GACNNNGTC 1 cut(s) 786
PkrI GCNGC 4 cut(s) 49, 99, 148, 1220
PleI GAGTC 2 cut(s) 695, 1021
PpsI GAGTC 2 cut(s) 695, 1021
PscI ACATGT 1 cut(s) 559
PshBI ATTAAT 1 cut(s) 438
Psp6I CCWGG 1 cut(s) 784
PspGI CCWGG 1 cut(s) 784
PspLI CGTACG 1 cut(s) 853
PspN4I GGNNCC 2 cut(s) 345, 1230
PspPI GGNCC 1 cut(s) 782
PsyI GACNNNGTC 1 cut(s) 786
RsaI GTAC 3 cut(s) 855, 1038, 1129
RsaNI GTAC 3 cut(s) 854, 1037, 1128
RseI CAYNNNNRTG 2 cut(s) 457, 1139
SaqAI TTAA 6 cut(s) 126, 438, 545, 738, 897, 1104
SatI GCNGC 4 cut(s) 48, 98, 147, 1219
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 1 cut(s) 782
SchI GAGTC 2 cut(s) 695, 1021
ScrFI CCNGG 3 cut(s) 317, 786, 1122
SexAI ACCWGGT 1 cut(s) 784
SfaNI GCATC 3 cut(s) 209, 597, 952
SfuI TTCGAA 1 cut(s) 942
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 2 cut(s) 457, 1139
SmlI CTYRAG 1 cut(s) 896
SmoI CTYRAG 1 cut(s) 896
SpeI ACTAGT 1 cut(s) 238
Sse9I AATT 7 cut(s) 298, 336, 435, 526, 795, 859, 1167
SsiI CCGC 2 cut(s) 8, 48
SspI AATATT 3 cut(s) 469, 694, 1066
SspMI CTAG 5 cut(s) 72, 219, 239, 891, 927
StyD4I CCNGG 3 cut(s) 315, 784, 1120
StyI CCWWGG 2 cut(s) 445, 455
TaaI ACNGT 6 cut(s) 311, 569, 613, 728, 1016, 1228
TaiI ACGT 1 cut(s) 855
TaqI TCGA 3 cut(s) 321, 942, 1025
TasI AATT 7 cut(s) 298, 336, 435, 526, 795, 859, 1167
TatI WGTACW 2 cut(s) 1036, 1127
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 3 cut(s) 170, 380, 1117
Tru1I TTAA 6 cut(s) 126, 438, 545, 738, 897, 1104
Tru9I TTAA 6 cut(s) 126, 438, 545, 738, 897, 1104
TseFI GTSAC 2 cut(s) 607, 1051
TseI GCWGC 3 cut(s) 97, 146, 1218
Tsp45I GTSAC 2 cut(s) 607, 1051
TspDTI ATGAA 7 cut(s) 375, 614, 686, 738, 745, 1157, 1272
TspGWI ACGGA 2 cut(s) 438, 888
Tth111I GACNNNGTC 1 cut(s) 786
Vha464I CTTAAG 1 cut(s) 896
VpaK11BI GGWCC 1 cut(s) 782
VspI ATTAAT 1 cut(s) 438
XceI RCATGY 2 cut(s) 563, 780
XmnI GAANNNNTTC 1 cut(s) 1078
XspI CTAG 5 cut(s) 72, 219, 239, 891, 927
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.