Rroxscaffold_6G00421350

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000579
Physical Location & Seq
Reverse (-)
42532688 .. 42534624
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421350.1

Sequence Viewer

Length: 936 bp
ATGGAAAAGCTGATGATTCGGGGAAAAATACCGGCGGGGAAAACATTCTTATTGCAGCCTACGAAATTTAAAGGTCCATGCAAATCAAAAAGTGTTCATGTTCAGGTGTTGGGGAAGGTGGTGGCACCCAAAACACCGGATGCTTGGAAACAGTGTGAATCAAATTACTGGCTTTCCTTTTCATATGTGGCAAACCTCAGAATGAATGGCGGTTCAGGAATAATCGACGGCCAGGGTTCATCTTGGTGGAGCAACGCTAACGAACAAAAGCTCTACATAGATGAGAAGAAGAAGTGTCAACGACCAAAGGCTCTACACTTCCATGGGTGTCATAATCTTCTACTAACTGGACTTACTCATGTAAATAGTCCAAAAGGTCATATTAGTATAAGCAACTGCAGCTATGTATATGTCGCCAATCTCACTATAACTGCACCTGAAGAGAGTCCAAACACCGATGGAATTGACATCTCAAACTCAAACCATGTCAACATTCATGCCTCTTATATTGGAACCGGTGATGATTGTATAGCTATCAATTCTGGATGTTCTAATATCAACATTACCAACATTGCATGTGGCTCAGGCCATGGAATTAGCGTGGGAAGCTTGGGAGAAAATGGGGCTTACGCAACAGTAGAAAATGTATCTGTGAAAACTTGTAGTTTCAGTAGAACTCAAAATGGAGTAAGAATCAAGACATGGCAGGGTGGATCTGGGAATATCCAAGGTACTTCAGCCAAAAAGGAGGCGATAAGATTAGACTGCAATCAGTTTTCGGGTTGTCGCAACATTGTAATGGAGTACATCAACATCACCTCGGATGTTCCTGGTAATAAGGGGATACAGGCATCATGCTACAACATCAATGGAATTTCTAGGGCCGAAAGCACCGTGCCGAGAGTACTTTGCCTGCAGTCCAAACTGGTCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

311

Amino Acids

33.54

Weight (kDa)

9.05

Isoelectric Point (pI)

34.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 9 - 243 7.5e-67 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 124
AciI CCGC 2 cut(s) 35, 210
AclWI GGATC 1 cut(s) 721
AcoI YGGCCR 1 cut(s) 229
AcsI RAATTY 2 cut(s) 65, 873
AcuI CTGAAG 2 cut(s) 459, 720
AfaI GTAC 3 cut(s) 733, 806, 906
AgeI ACCGGT 1 cut(s) 515
AjnI CCWGG 2 cut(s) 231, 829
AluBI AGCT 5 cut(s) 10, 271, 402, 533, 609
AluI AGCT 5 cut(s) 10, 271, 402, 533, 609
AlwI GGATC 1 cut(s) 721
AoxI GGCC 3 cut(s) 229, 586, 882
ApeKI GCWGC 2 cut(s) 55, 399
ApoI RAATTY 2 cut(s) 65, 873
AsiGI ACCGGT 1 cut(s) 515
Asp700I GAANNNNTTC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 74, 882, 928
AsuHPI GGTGA 2 cut(s) 530, 808
AvaII GGWCC 2 cut(s) 74, 928
BanI GGYRCC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 67, 411
BccI CCATC 1 cut(s) 452
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 2 cut(s) 233, 831
BciVI GTATCC 1 cut(s) 837
BfaI CTAG 1 cut(s) 879
BfmI CTRYAG 2 cut(s) 397, 914
BfuI GTATCC 1 cut(s) 837
BisI GCNGC 2 cut(s) 56, 400
BlsI GCNGC 2 cut(s) 57, 401
BmcAI AGTACT 1 cut(s) 906
Bme1390I CCNGG 2 cut(s) 233, 831
Bme18I GGWCC 2 cut(s) 74, 928
BmgT120I GGNCC 3 cut(s) 74, 882, 928
BmiI GGNNCC 2 cut(s) 126, 514
BmrFI CCNGG 2 cut(s) 233, 831
BmsI GCATC 2 cut(s) 130, 860
Bpu10I CCTNAGC 1 cut(s) 583
BsaJI CCNNGG 5 cut(s) 232, 322, 589, 727, 819
BsaWI WCCGGW 2 cut(s) 136, 515
Bse118I RCCGGY 2 cut(s) 31, 515
Bse1I ACTGG 4 cut(s) 173, 352, 930, 931
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 233, 831
BseDI CCNNGG 5 cut(s) 232, 322, 589, 727, 819
BseGI GGATG 3 cut(s) 145, 551, 829
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 2 cut(s) 211, 597
BseNI ACTGG 4 cut(s) 173, 352, 930, 931
BseXI GCAGC 2 cut(s) 67, 411
BsgI GTGCAG 1 cut(s) 417
BshFI GGCC 3 cut(s) 231, 588, 884
BshNI GGYRCC 1 cut(s) 124
BshTI ACCGGT 1 cut(s) 515
BsiSI CCGG 3 cut(s) 32, 137, 516
BsnI GGCC 3 cut(s) 231, 588, 884
Bsp143I GATC 1 cut(s) 713
Bsp19I CCATGG 2 cut(s) 322, 589
BspACI CCGC 2 cut(s) 35, 210
BspANI GGCC 3 cut(s) 231, 588, 884
BspCNI CTCAG 2 cut(s) 210, 596
BspLI GGNNCC 2 cut(s) 126, 514
BspMAI CTGCAG 2 cut(s) 401, 918
BspPI GGATC 1 cut(s) 721
BspT107I GGYRCC 1 cut(s) 124
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 2 cut(s) 31, 515
BsrI ACTGG 4 cut(s) 173, 352, 930, 931
BssAI RCCGGY 2 cut(s) 31, 515
BssECI CCNNGG 5 cut(s) 232, 322, 589, 727, 819
BssMI GATC 1 cut(s) 713
BssT1I CCWWGG 3 cut(s) 322, 589, 727
Bst2UI CCWGG 2 cut(s) 233, 831
Bst4CI ACNGT 3 cut(s) 153, 637, 895
Bst6I CTCTTC 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 914
BstDEI CTNAG 2 cut(s) 197, 583
BstDSI CCRYGG 2 cut(s) 322, 589
BstF5I GGATG 3 cut(s) 145, 551, 829
BstKTI GATC 1 cut(s) 716
BstMBI GATC 1 cut(s) 713
BstMWI GCNNNNNNNGC 2 cut(s) 399, 606
BstNI CCWGG 2 cut(s) 233, 831
BstNSI RCATGY 1 cut(s) 579
BstSCI CCNGG 2 cut(s) 231, 829
BstSFI CTRYAG 2 cut(s) 397, 914
BstV1I GCAGC 2 cut(s) 67, 411
BstX2I RGATCY 1 cut(s) 713
BstYI RGATCY 1 cut(s) 713
BsuI GTATCC 1 cut(s) 837
BsuRI GGCC 3 cut(s) 231, 588, 884
BtgI CCRYGG 2 cut(s) 322, 589
BtsCI GGATG 3 cut(s) 145, 551, 829
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 1 cut(s) 914
Cfr10I RCCGGY 2 cut(s) 31, 515
Cfr13I GGNCC 3 cut(s) 74, 882, 928
Csp6I GTAC 3 cut(s) 732, 805, 905
CspAI ACCGGT 1 cut(s) 515
CviQI GTAC 3 cut(s) 732, 805, 905
DdeI CTNAG 2 cut(s) 197, 583
DpnI GATC 1 cut(s) 715
DpnII GATC 1 cut(s) 713
DraI TTTAAA 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco130I CCWWGG 3 cut(s) 322, 589, 727
Eco47I GGWCC 2 cut(s) 74, 928
Eco57I CTGAAG 2 cut(s) 459, 720
EcoRII CCWGG 2 cut(s) 231, 829
EcoT14I CCWWGG 3 cut(s) 322, 589, 727
ErhI CCWWGG 3 cut(s) 322, 589, 727
FauI CCCGC 1 cut(s) 28
FauNDI CATATG 1 cut(s) 184
Fnu4HI GCNGC 2 cut(s) 56, 400
FokI GGATG 3 cut(s) 152, 558, 836
Fsp4HI GCNGC 2 cut(s) 56, 400
FspBI CTAG 1 cut(s) 879
GluI GCNGC 2 cut(s) 56, 400
HaeIII GGCC 3 cut(s) 231, 588, 884
HapII CCGG 3 cut(s) 32, 137, 516
HincII GTYRAC 2 cut(s) 299, 490
HindII GTYRAC 2 cut(s) 299, 490
HindIII AAGCTT 1 cut(s) 607
HinfI GANTC 4 cut(s) 16, 158, 445, 693
HpaII CCGG 3 cut(s) 32, 137, 516
HphI GGTGA 2 cut(s) 530, 808
Hpy166II GTNNAC 2 cut(s) 299, 490
Hpy188I TCNGA 2 cut(s) 200, 823
Hpy188III TCNNGA 3 cut(s) 216, 543, 697
Hpy8I GTNNAC 2 cut(s) 299, 490
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 1 cut(s) 109
HpyCH4III ACNGT 3 cut(s) 153, 637, 895
HpyCH4V TGCA 7 cut(s) 55, 81, 399, 434, 575, 768, 916
HpyF10VI GCNNNNNNNGC 2 cut(s) 399, 606
HpyF3I CTNAG 2 cut(s) 197, 583
Kzo9I GATC 1 cut(s) 713
LmnI GCTCC 1 cut(s) 249
Lsp1109I GCAGC 2 cut(s) 67, 411
LweI GCATC 2 cut(s) 130, 860
MaeI CTAG 1 cut(s) 879
MalI GATC 1 cut(s) 715
MboI GATC 1 cut(s) 713
MboII GAAGA 4 cut(s) 298, 301, 329, 452
MflI RGATCY 1 cut(s) 713
MluCI AATT 6 cut(s) 65, 163, 462, 538, 594, 873
MlyI GAGTC 1 cut(s) 454
MnlI CCTC 4 cut(s) 206, 511, 742, 829
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 3 cut(s) 244, 321, 797
MspI CCGG 3 cut(s) 32, 137, 516
MspR9I CCNGG 2 cut(s) 233, 831
MvaI CCWGG 2 cut(s) 233, 831
MwoI GCNNNNNNNGC 2 cut(s) 399, 606
NcoI CCATGG 2 cut(s) 322, 589
NdeI CATATG 1 cut(s) 184
NdeII GATC 1 cut(s) 713
NlaIV GGNNCC 2 cut(s) 126, 514
NmeAIII GCCGAG 1 cut(s) 924
NspI RCATGY 1 cut(s) 579
PdmI GAANNNNTTC 1 cut(s) 44
PfeI GAWTC 3 cut(s) 16, 158, 693
PinAI ACCGGT 1 cut(s) 515
PkrI GCNGC 2 cut(s) 57, 401
PleI GAGTC 1 cut(s) 453
PpsI GAGTC 1 cut(s) 453
Psp6I CCWGG 2 cut(s) 231, 829
PspGI CCWGG 2 cut(s) 231, 829
PspN4I GGNNCC 2 cut(s) 126, 514
PspPI GGNCC 3 cut(s) 74, 882, 928
PstI CTGCAG 2 cut(s) 401, 918
PsuI RGATCY 1 cut(s) 713
RsaI GTAC 3 cut(s) 733, 806, 906
RsaNI GTAC 3 cut(s) 732, 805, 905
RseI CAYNNNNRTG 3 cut(s) 244, 321, 797
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 2 cut(s) 56, 400
Sau3AI GATC 1 cut(s) 713
Sau96I GGNCC 3 cut(s) 74, 882, 928
ScaI AGTACT 1 cut(s) 906
SchI GAGTC 1 cut(s) 454
ScrFI CCNGG 2 cut(s) 233, 831
SfaNI GCATC 2 cut(s) 130, 860
SfcI CTRYAG 2 cut(s) 397, 914
SinI GGWCC 2 cut(s) 74, 928
SmiMI CAYNNNNRTG 3 cut(s) 244, 321, 797
Sse9I AATT 6 cut(s) 65, 163, 462, 538, 594, 873
SsiI CCGC 2 cut(s) 35, 210
SspMI CTAG 1 cut(s) 879
StyD4I CCNGG 2 cut(s) 231, 829
StyI CCWWGG 3 cut(s) 322, 589, 727
TaaI ACNGT 3 cut(s) 153, 637, 895
TaqI TCGA 1 cut(s) 225
TasI AATT 6 cut(s) 65, 163, 462, 538, 594, 873
TatI WGTACW 2 cut(s) 804, 904
TfiI GAWTC 3 cut(s) 16, 158, 693
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 158
TseI GCWGC 2 cut(s) 55, 399
TspDTI ATGAA 5 cut(s) 86, 171, 218, 228, 485
TspRI CASTG 1 cut(s) 158
VpaK11BI GGWCC 2 cut(s) 74, 928
XapI RAATTY 2 cut(s) 65, 873
XceI RCATGY 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 44
XspI CTAG 1 cut(s) 879
ZrmI AGTACT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.