Rw3G007220

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000579
Physical Location & Seq
Forward (+)
6671282 .. 6673840
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G007220.1

Sequence Viewer

Length: 1188 bp
ATGCAGTACATACTTGTGAGCTTTTTGATATTTTGCATATCTTCATCTCATTTAAGCGACATTGTATATGGAAAAAAAACATATGATGTTCTTAGTTATGGTGCTGTTGGAGATGGAAACGCTGATGATTCAAAACGTGGGAAGATTTTATGTGGAGCATCTGAAACTTATGATGTACCAACACTTCAGATACCGGCGGGGAAAACATTCTTATTGCAGCCTACGAAATTTAAAGGTCCATGCAAATCAAAAAGTGTTCATGTTCAGGTGTTGGGGAAGGTGGTGGCACCCAAAACACCGGATGCTTGGAAACAGTGTGAATCAAATAACTGGCTTTCCTTTTCAAATGTGGCAAACCTCAGAATGAATGGCGGTTCAGGAATAATCGACGGCCAGGGTTCATCTTGGTGGAGCAACGCTAACGAACAAAAGCTCCACATAGATGACAAGAAGAAGTGTCAACGACCAAAGGCTCTACACTTCCATGGATGTCATAATCTTCTACTAACTGGACTTACTCATGTAAATAGTCCGAAAGGTCATATTAGTATAAGCAACTGCAGCTATGTATATGTCGCCAATCTCACTATAACTGCACCTGAAGAGAGTCCAAACACCGATGGAATTGACATCTCAAACTCAAATCATGTCAACATTCATGCCTCTTATATTGGAACCGGTGATGATTGTATAGCTATCAATTCTGGATGTTCTAATATCAACATTACCAACATTGCATGTGGCCCAGGCCATGGAATTAGCGTGGGAAGCTTGGGAGAAAATGGGGCTTACGCAACAGTAGAAAATGTATCTGTGAAAAATTGTAATTTCAGTAGAACTCAAAATGGAGTAAGAATCAAGACATGGCAGGGTGGATCTGGGTACGCCAAGAATATAACCTTTGAACAAATCACACTTCATGCCACTAAAAACCCAATTATCATCGACCAATATTATTGTAATGGAGCTCACAATTGTAAAAACAAGACCTCAGCTGTCAAGGTGAGTGATGTGAGATACAGGAATATCCAAGGCACTTCAGCAAAAATGGAGGCGATAAGATTAGACTGCAATCAGTTTTCGGGTTGTCGCAACATTGTAATGGAGCACATCAACATCACCTCGAATCAAATGAGCAATACAATTATCGTACGGTGCGAATGCATAGCCATGGTTTTGTCATCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

395

Amino Acids

42.85

Weight (kDa)

8.66

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 61 - 380 6.6e-94 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 1 cut(s) 752
AciI CCGC 2 cut(s) 197, 372
AclWI GGATC 1 cut(s) 883
AcoI YGGCCR 1 cut(s) 391
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 3 cut(s) 170, 621, 1023
AfaI GTAC 4 cut(s) 8, 177, 884, 1152
AfiI CCNNNNNNNGG 1 cut(s) 752
AgeI ACCGGT 1 cut(s) 677
AgsI TTSAA 3 cut(s) 132, 345, 905
AjnI CCWGG 2 cut(s) 393, 745
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
AluBI AGCT 7 cut(s) 21, 433, 564, 695, 771, 968, 995
AluI AGCT 7 cut(s) 21, 433, 564, 695, 771, 968, 995
Alw21I GWGCWC 2 cut(s) 970, 1110
AlwI GGATC 1 cut(s) 883
AoxI GGCC 3 cut(s) 391, 742, 748
ApeKI GCWGC 2 cut(s) 217, 561
ApoI RAATTY 1 cut(s) 227
AsiGI ACCGGT 1 cut(s) 677
Asp700I GAANNNNTTC 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 236, 743
AsuHPI GGTGA 3 cut(s) 692, 1015, 1111
AvaII GGWCC 1 cut(s) 236
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 970
BarI GAAGNNNNNNTAC 2 cut(s) 168, 200
Bbv12I GWGCWC 2 cut(s) 970, 1110
BbvCI CCTCAGC 1 cut(s) 991
BbvI GCAGC 2 cut(s) 229, 573
BccI CCATC 2 cut(s) 107, 614
BceAI ACGGC 1 cut(s) 406
BciT130I CCWGG 2 cut(s) 395, 747
BfmI CTRYAG 1 cut(s) 559
BisI GCNGC 2 cut(s) 218, 562
BlsI GCNGC 2 cut(s) 219, 563
Bme1390I CCNGG 2 cut(s) 395, 747
Bme18I GGWCC 1 cut(s) 236
BmgT120I GGNCC 2 cut(s) 236, 743
BmiI GGNNCC 2 cut(s) 288, 676
BmrFI CCNGG 2 cut(s) 395, 747
BmsI GCATC 2 cut(s) 167, 292
Bpu10I CCTNAGC 1 cut(s) 991
BsaJI CCNNGG 6 cut(s) 394, 484, 745, 751, 1030, 1170
BsaWI WCCGGW 2 cut(s) 298, 677
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 1 cut(s) 752
Bse118I RCCGGY 2 cut(s) 193, 677
Bse1I ACTGG 2 cut(s) 335, 514
Bse3DI GCAATG 1 cut(s) 732
BseBI CCWGG 2 cut(s) 395, 747
BseDI CCNNGG 6 cut(s) 394, 484, 745, 751, 1030, 1170
BseGI GGATG 3 cut(s) 307, 494, 713
BseLI CCNNNNNNNGG 1 cut(s) 752
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 373, 1005
BseNI ACTGG 2 cut(s) 335, 514
BseXI GCAGC 2 cut(s) 229, 573
BsgI GTGCAG 1 cut(s) 579
BshFI GGCC 3 cut(s) 393, 744, 750
BshNI GGYRCC 1 cut(s) 286
BshTI ACCGGT 1 cut(s) 677
BsiHKAI GWGCWC 2 cut(s) 970, 1110
BsiSI CCGG 3 cut(s) 194, 299, 678
BsiWI CGTACG 1 cut(s) 1150
BslI CCNNNNNNNGG 1 cut(s) 752
BsmI GAATGC 1 cut(s) 1166
BsnI GGCC 3 cut(s) 393, 744, 750
Bsp1286I GDGCHC 2 cut(s) 970, 1110
Bsp143I GATC 1 cut(s) 875
Bsp19I CCATGG 3 cut(s) 484, 751, 1170
BspACI CCGC 2 cut(s) 197, 372
BspANI GGCC 3 cut(s) 393, 744, 750
BspCNI CTCAG 2 cut(s) 372, 1004
BspLI GGNNCC 2 cut(s) 288, 676
BspMAI CTGCAG 1 cut(s) 563
BspPI GGATC 1 cut(s) 883
BspT107I GGYRCC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 732
BsrFI RCCGGY 2 cut(s) 193, 677
BsrI ACTGG 2 cut(s) 335, 514
BssAI RCCGGY 2 cut(s) 193, 677
BssECI CCNNGG 6 cut(s) 394, 484, 745, 751, 1030, 1170
BssMI GATC 1 cut(s) 875
BssT1I CCWWGG 4 cut(s) 484, 751, 1030, 1170
Bst2UI CCWGG 2 cut(s) 395, 747
Bst4CI ACNGT 3 cut(s) 315, 799, 1155
Bst6I CTCTTC 1 cut(s) 597
BstDEI CTNAG 3 cut(s) 92, 359, 991
BstDSI CCRYGG 3 cut(s) 484, 751, 1170
BstF5I GGATG 3 cut(s) 307, 494, 713
BstKTI GATC 1 cut(s) 878
BstMBI GATC 1 cut(s) 875
BstMWI GCNNNNNNNGC 2 cut(s) 561, 768
BstNI CCWGG 2 cut(s) 395, 747
BstNSI RCATGY 1 cut(s) 741
BstSCI CCNGG 2 cut(s) 393, 745
BstSFI CTRYAG 1 cut(s) 559
BstV1I GCAGC 2 cut(s) 229, 573
BstX2I RGATCY 1 cut(s) 875
BstYI RGATCY 1 cut(s) 875
BsuRI GGCC 3 cut(s) 393, 744, 750
BtgI CCRYGG 3 cut(s) 484, 751, 1170
BtsCI GGATG 3 cut(s) 307, 494, 713
BtsIMutI CAGTG 1 cut(s) 320
Cfr10I RCCGGY 2 cut(s) 193, 677
Cfr13I GGNCC 2 cut(s) 236, 743
Csp6I GTAC 4 cut(s) 7, 176, 883, 1151
CspAI ACCGGT 1 cut(s) 677
CviQI GTAC 4 cut(s) 7, 176, 883, 1151
DdeI CTNAG 3 cut(s) 92, 359, 991
DpnI GATC 1 cut(s) 877
DpnII GATC 1 cut(s) 875
DraI TTTAAA 1 cut(s) 232
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 1 cut(s) 597
EarI CTCTTC 1 cut(s) 597
Ecl136II GAGCTC 1 cut(s) 968
Eco130I CCWWGG 4 cut(s) 484, 751, 1030, 1170
Eco24I GRGCYC 1 cut(s) 970
Eco47I GGWCC 1 cut(s) 236
Eco53kI GAGCTC 1 cut(s) 968
Eco57I CTGAAG 3 cut(s) 170, 621, 1023
EcoICRI GAGCTC 1 cut(s) 968
EcoRII CCWGG 2 cut(s) 393, 745
EcoT14I CCWWGG 4 cut(s) 484, 751, 1030, 1170
EcoT22I ATGCAT 1 cut(s) 1166
EcoT38I GRGCYC 1 cut(s) 970
ErhI CCWWGG 4 cut(s) 484, 751, 1030, 1170
FauI CCCGC 1 cut(s) 190
FauNDI CATATG 1 cut(s) 82
Fnu4HI GCNGC 2 cut(s) 218, 562
FokI GGATG 3 cut(s) 314, 501, 720
FriOI GRGCYC 1 cut(s) 970
Fsp4HI GCNGC 2 cut(s) 218, 562
GluI GCNGC 2 cut(s) 218, 562
HaeIII GGCC 3 cut(s) 393, 744, 750
HapII CCGG 3 cut(s) 194, 299, 678
HincII GTYRAC 2 cut(s) 461, 652
HindII GTYRAC 2 cut(s) 461, 652
HindIII AAGCTT 1 cut(s) 769
HinfI GANTC 5 cut(s) 128, 320, 607, 855, 1126
HpaII CCGG 3 cut(s) 194, 299, 678
HphI GGTGA 3 cut(s) 692, 1015, 1111
Hpy166II GTNNAC 2 cut(s) 461, 652
Hpy188I TCNGA 4 cut(s) 163, 189, 362, 534
Hpy188III TCNNGA 3 cut(s) 378, 705, 859
Hpy8I GTNNAC 2 cut(s) 461, 652
Hpy99I CGWCG 1 cut(s) 392
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 3 cut(s) 315, 799, 1155
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 9 cut(s) 4, 36, 217, 243, 561, 596, 737, 1071, 1164
HpyF10VI GCNNNNNNNGC 2 cut(s) 561, 768
HpyF3I CTNAG 3 cut(s) 92, 359, 991
HpySE526I ACGT 1 cut(s) 136
Kzo9I GATC 1 cut(s) 875
LmnI GCTCC 5 cut(s) 155, 411, 438, 965, 1105
Lsp1109I GCAGC 2 cut(s) 229, 573
LweI GCATC 2 cut(s) 167, 292
MaeII ACGT 1 cut(s) 136
MalI GATC 1 cut(s) 877
MboI GATC 1 cut(s) 875
MboII GAAGA 5 cut(s) 33, 154, 463, 491, 614
MfeI CAATTG 1 cut(s) 973
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 2 cut(s) 970, 1110
MluCI AATT 9 cut(s) 227, 624, 700, 756, 820, 826, 936, 973, 1143
MlyI GAGTC 1 cut(s) 616
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 5 cut(s) 368, 673, 1000, 1045, 1132
Mph1103I ATGCAT 1 cut(s) 1166
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 2 cut(s) 53, 231
MslI CAYNNNNRTG 6 cut(s) 14, 406, 441, 483, 1100, 1169
MspA1I CMGCKG 1 cut(s) 995
MspI CCGG 3 cut(s) 194, 299, 678
MspR9I CCNGG 2 cut(s) 395, 747
MunI CAATTG 1 cut(s) 973
Mva1269I GAATGC 1 cut(s) 1166
MvaI CCWGG 2 cut(s) 395, 747
MwoI GCNNNNNNNGC 2 cut(s) 561, 768
NcoI CCATGG 3 cut(s) 484, 751, 1170
NdeI CATATG 1 cut(s) 82
NdeII GATC 1 cut(s) 875
NlaIV GGNNCC 2 cut(s) 288, 676
NsiI ATGCAT 1 cut(s) 1166
NspI RCATGY 1 cut(s) 741
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PctI GAATGC 1 cut(s) 1166
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 4 cut(s) 128, 320, 855, 1126
Pfl23II CGTACG 1 cut(s) 1150
PflMI CCANNNNNTGG 1 cut(s) 752
PinAI ACCGGT 1 cut(s) 677
PkrI GCNGC 2 cut(s) 219, 563
PleI GAGTC 1 cut(s) 615
PpsI GAGTC 1 cut(s) 615
Psp124BI GAGCTC 1 cut(s) 970
Psp6I CCWGG 2 cut(s) 393, 745
PspGI CCWGG 2 cut(s) 393, 745
PspLI CGTACG 1 cut(s) 1150
PspN4I GGNNCC 2 cut(s) 288, 676
PspPI GGNCC 2 cut(s) 236, 743
PstI CTGCAG 1 cut(s) 563
PsuI RGATCY 1 cut(s) 875
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 4 cut(s) 8, 177, 884, 1152
RsaNI GTAC 4 cut(s) 7, 176, 883, 1151
RseI CAYNNNNRTG 6 cut(s) 14, 406, 441, 483, 1100, 1169
SacI GAGCTC 1 cut(s) 970
SaqAI TTAA 2 cut(s) 53, 231
SatI GCNGC 2 cut(s) 218, 562
Sau3AI GATC 1 cut(s) 875
Sau96I GGNCC 2 cut(s) 236, 743
SchI GAGTC 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 395, 747
SduI GDGCHC 2 cut(s) 970, 1110
SfaNI GCATC 2 cut(s) 167, 292
SfcI CTRYAG 1 cut(s) 559
SinI GGWCC 1 cut(s) 236
SmiMI CAYNNNNRTG 6 cut(s) 14, 406, 441, 483, 1100, 1169
Sse9I AATT 9 cut(s) 227, 624, 700, 756, 820, 826, 936, 973, 1143
SsiI CCGC 2 cut(s) 197, 372
SspI AATATT 1 cut(s) 953
SstI GAGCTC 1 cut(s) 970
StyD4I CCNGG 2 cut(s) 393, 745
StyI CCWWGG 4 cut(s) 484, 751, 1030, 1170
TaaI ACNGT 3 cut(s) 315, 799, 1155
TaiI ACGT 1 cut(s) 139
TaqI TCGA 3 cut(s) 387, 945, 1124
TasI AATT 9 cut(s) 227, 624, 700, 756, 820, 826, 936, 973, 1143
TatI WGTACW 1 cut(s) 6
TfiI GAWTC 4 cut(s) 128, 320, 855, 1126
Tru1I TTAA 2 cut(s) 53, 231
Tru9I TTAA 2 cut(s) 53, 231
TscAI CASTG 1 cut(s) 320
TseI GCWGC 2 cut(s) 217, 561
TspDTI ATGAA 6 cut(s) 33, 248, 380, 390, 647, 908
TspRI CASTG 1 cut(s) 320
Van91I CCANNNNNTGG 1 cut(s) 752
VpaK11BI GGWCC 1 cut(s) 236
XapI RAATTY 1 cut(s) 227
XceI RCATGY 1 cut(s) 741
XmnI GAANNNNTTC 1 cut(s) 206
Zsp2I ATGCAT 1 cut(s) 1166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.