AT4G32380

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000579
Physical Location & Seq
Forward (+)
15632712 .. 15635494
2783 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G32380.1

Sequence Viewer

Length: 1362 bp
ATGGGTTTTGAGATACTGCTCTCAGTGCTTCTAATTGCATCATACCATTTACAGTACGGAAATGGTCGAATGATTCTAACCATGAAAGATTTTATATCTAATTCTAACTCTACAAATGTTGATCATTCTCAGGCATTTCAAAACGCATGGAGAGCGTTATGTGGAGGGAAAGGTGGATCAGCCTCGTTTGTAATACAAGCCCATGAAACATATACCATACAACCACAATTGCTTGAAGGTCCTTGCATGCCTCGTAATATTCATATTCAGATTGATGGAACAATTGAAGCTCCGAAGATGGCAAACGACTGGGGAAGGAATAAACTTGACTGTTGGTTATGTTTTGAAAAGGTGACAGGCCTCGTTCTCACTGGATCCGGTGTCCTCAATACCCATGGAGAAAGCTGGTGGTCTTCTGTTGCTCTCCAATCTCGACCAGTGGCAGTAAGATTTTTTGGATGCCAAAACATTTTATATAATGGATTAACTCAGATCAATAGTCCAAGGAATCACATAACGATTTTGGATAGCAACAACGCAACTTTATCAAATCTTCATCTAATTGCCCCTGCAAGTAGTCCAAATACTGATGGCATTGATATCTCTCATTCACAAAATATCAATATTATGAGCTCCACAATCAAAACCGGTGATGACTGTGTTGCAATTAAACGTAATTCATACAATATTAACGTAACTTACGTGACATGTGGTCCGGGTCATGGCATAAGTATCGGAAGTTTGGGGGAAGGAGGGGCATCTGAGGTAGTACAAAATGTGAATGTCAGACATTGCACCTTCACTGGAACACAAAATGGTGCAAGAATCAAGACTTGGCCTGGAGGACAAGGGTTTGTCAAAAATATTTTGTACGAAGATATTACTCTAATAAATGCCAACTTCCCCATCATCATCGATCAACAATATCGTGATAATGCTGGTCAATATAAACAATCAGCAGGGGCTACTGCTGTGAAAGTGAGCGACGTGACGTTTAGATCATTCACAGGGACTTGTGCCGCTCCTATTGCAATAAAACTAGATTGTGACCCGAATACAGGTTGTGACAACATCGTGATGGAACAAATCAATATCGCTTCTTCATCTCCGAAAACGCCGCTCACCTCGTACTGCAAATTTGCGCATGTCGTTAGTCGCTTTGTCTCCATTCCCATAACCTGTAGTTTTCATACTGAAGATTCTCAACCTGCGTCACTGAATCCTCAACCTTCCGCACCGTATGCTATATCCCCGACGACACCGCATACTCAGCCTCATGCACCAACTACTCAACCTCCACTGTTCTTTCGTTTCTATACAAATTTCAAAGCCTTCTTAGGAAGGTTGGGTCGTAACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

453

Amino Acids

49.27

Weight (kDa)

7.91

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 61 - 379 2.7e-80 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1143
Acc36I ACCTGC 1 cut(s) 1216
AccBSI CCGCTC 2 cut(s) 1022, 1120
AciI CCGC 4 cut(s) 1020, 1118, 1233, 1262
AclWI GGATC 3 cut(s) 184, 369, 382
AcsI RAATTY 2 cut(s) 1136, 1321
AcuI CTGAAG 1 cut(s) 1215
AfaI GTAC 4 cut(s) 56, 771, 872, 1130
AfiI CCNNNNNNNGG 2 cut(s) 722, 1058
AflIII ACRYGT 1 cut(s) 707
AgeI ACCGGT 1 cut(s) 647
AgsI TTSAA 5 cut(s) 140, 236, 287, 347, 1327
AhdI GACNNNNNGTC 1 cut(s) 711
AjiI CACGTC 1 cut(s) 988
AjnI CCWGG 1 cut(s) 838
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AluBI AGCT 3 cut(s) 290, 405, 633
AluI AGCT 3 cut(s) 290, 405, 633
Alw21I GWGCWC 1 cut(s) 635
Alw26I GTCTC 1 cut(s) 1168
AlwI GGATC 3 cut(s) 184, 369, 382
AoxI GGCC 2 cut(s) 358, 836
ApoI RAATTY 2 cut(s) 1136, 1321
AsiGI ACCGGT 1 cut(s) 647
AspLEI GCGC 1 cut(s) 1144
AspS9I GGNCC 2 cut(s) 239, 713
AsuC2I CCSGG 1 cut(s) 717
AsuHPI GGTGA 3 cut(s) 364, 662, 1114
AvaII GGWCC 2 cut(s) 239, 713
BamHI GGATCC 1 cut(s) 374
BanII GRGCYC 1 cut(s) 635
BbsI GAAGAC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 635
BccI CCATC 5 cut(s) 269, 292, 584, 914, 1072
BcgI CGANNNNNNTGC 2 cut(s) 715, 749
BciT130I CCWGG 1 cut(s) 840
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 1 cut(s) 717
BcoDI GTCTC 1 cut(s) 1168
BfaI CTAG 2 cut(s) 1040, 1360
BfmI CTRYAG 1 cut(s) 1180
BfuAI ACCTGC 1 cut(s) 1216
BisI GCNGC 2 cut(s) 1020, 1118
BlsI GCNGC 2 cut(s) 1021, 1119
Bme1390I CCNGG 2 cut(s) 717, 840
Bme18I GGWCC 2 cut(s) 239, 713
BmeRI GACNNNNNGTC 1 cut(s) 711
BmgBI CACGTC 1 cut(s) 988
BmgT120I GGNCC 2 cut(s) 239, 713
BmiI GGNNCC 1 cut(s) 376
BmrFI CCNGG 2 cut(s) 717, 840
BmrI ACTGGG 1 cut(s) 319
BmsI GCATC 3 cut(s) 47, 449, 767
BmuI ACTGGG 1 cut(s) 319
BpiI GAAGAC 1 cut(s) 405
BpmI CTGGAG 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 717
Bsa29I ATCGAT 1 cut(s) 915
BsaAI YACGTR 1 cut(s) 703
BsaJI CCNNGG 2 cut(s) 394, 503
BsaWI WCCGGW 2 cut(s) 377, 647
BsaXI ACNNNNNCTCC 2 cut(s) 1279, 1309
Bsc4I CCNNNNNNNGG 2 cut(s) 722, 1058
Bse118I RCCGGY 1 cut(s) 647
Bse1I ACTGG 4 cut(s) 314, 376, 437, 808
Bse3DI GCAATG 1 cut(s) 790
BseBI CCWGG 1 cut(s) 840
BseCI ATCGAT 1 cut(s) 915
BseDI CCNNGG 2 cut(s) 394, 503
BseGI GGATG 1 cut(s) 464
BseLI CCNNNNNNNGG 2 cut(s) 722, 1058
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 5 cut(s) 36, 143, 503, 753, 1283
BseNI ACTGG 4 cut(s) 314, 376, 437, 808
BshFI GGCC 2 cut(s) 360, 838
BshTI ACCGGT 1 cut(s) 647
BshVI ATCGAT 1 cut(s) 915
BsiHKAI GWGCWC 1 cut(s) 635
BsiSI CCGG 3 cut(s) 378, 648, 716
BslFI GGGAC 1 cut(s) 1024
BslI CCNNNNNNNGG 2 cut(s) 722, 1058
BsmAI GTCTC 1 cut(s) 1168
BsmFI GGGAC 1 cut(s) 1024
BsnI GGCC 2 cut(s) 360, 838
Bsp1286I GDGCHC 1 cut(s) 635
Bsp143I GATC 6 cut(s) 121, 176, 374, 492, 916, 998
Bsp19I CCATGG 1 cut(s) 394
BspACI CCGC 4 cut(s) 1020, 1118, 1233, 1262
BspANI GGCC 2 cut(s) 360, 838
BspCNI CTCAG 5 cut(s) 35, 142, 502, 754, 1282
BspDI ATCGAT 1 cut(s) 915
BspLI GGNNCC 1 cut(s) 376
BspMI ACCTGC 1 cut(s) 1216
BspPI GGATC 3 cut(s) 184, 369, 382
BsrBI CCGCTC 2 cut(s) 1022, 1120
BsrDI GCAATG 1 cut(s) 790
BsrFI RCCGGY 1 cut(s) 647
BsrI ACTGG 4 cut(s) 314, 376, 437, 808
BssAI RCCGGY 1 cut(s) 647
BssECI CCNNGG 2 cut(s) 394, 503
BssMI GATC 6 cut(s) 121, 176, 374, 492, 916, 998
BssT1I CCWWGG 2 cut(s) 394, 503
Bst2UI CCWGG 1 cut(s) 840
Bst4CI ACNGT 5 cut(s) 54, 332, 659, 1239, 1302
BstAPI GCANNNNNTGC 1 cut(s) 1241
BstBAI YACGTR 1 cut(s) 703
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 6 cut(s) 22, 129, 489, 762, 1269, 1336
BstDSI CCRYGG 1 cut(s) 394
BstF5I GGATG 1 cut(s) 464
BstHHI GCGC 1 cut(s) 1144
BstKTI GATC 6 cut(s) 124, 179, 377, 495, 919, 1001
BstMAI GTCTC 1 cut(s) 1168
BstMBI GATC 6 cut(s) 121, 176, 374, 492, 916, 998
BstMWI GCNNNNNNNGC 5 cut(s) 25, 152, 1028, 1241, 1270
BstNI CCWGG 1 cut(s) 840
BstNSI RCATGY 3 cut(s) 250, 711, 1148
BstSCI CCNGG 2 cut(s) 715, 838
BstSFI CTRYAG 1 cut(s) 1180
BstV2I GAAGAC 1 cut(s) 405
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
Bsu15I ATCGAT 1 cut(s) 915
BsuRI GGCC 2 cut(s) 360, 838
BsuTUI ATCGAT 1 cut(s) 915
BtgI CCRYGG 1 cut(s) 394
BtrI CACGTC 1 cut(s) 988
BtsCI GGATG 1 cut(s) 464
BtsIMutI CAGTG 6 cut(s) 30, 369, 444, 801, 1214, 1298
BveI ACCTGC 1 cut(s) 1216
Cac8I GCNNGC 1 cut(s) 248
CfoI GCGC 1 cut(s) 1144
Cfr10I RCCGGY 1 cut(s) 647
Cfr13I GGNCC 2 cut(s) 239, 713
ClaI ATCGAT 1 cut(s) 915
CseI GACGC 1 cut(s) 1200
Csp6I GTAC 4 cut(s) 55, 770, 871, 1129
CspAI ACCGGT 1 cut(s) 647
CviAII CATG 9 cut(s) 82, 147, 203, 247, 395, 708, 722, 1145, 1277
CviQI GTAC 4 cut(s) 55, 770, 871, 1129
DdeI CTNAG 6 cut(s) 22, 129, 489, 762, 1269, 1336
DpnI GATC 6 cut(s) 123, 178, 376, 494, 918, 1000
DpnII GATC 6 cut(s) 121, 176, 374, 492, 916, 998
DriI GACNNNNNGTC 1 cut(s) 711
Eam1105I GACNNNNNGTC 1 cut(s) 711
Ecl136II GAGCTC 1 cut(s) 633
Eco130I CCWWGG 2 cut(s) 394, 503
Eco147I AGGCCT 1 cut(s) 360
Eco24I GRGCYC 1 cut(s) 635
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 2 cut(s) 239, 713
Eco53kI GAGCTC 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 1215
EcoICRI GAGCTC 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 838
EcoRV GATATC 1 cut(s) 601
EcoT14I CCWWGG 2 cut(s) 394, 503
EcoT38I GRGCYC 1 cut(s) 635
ErhI CCWWGG 2 cut(s) 394, 503
FaeI CATG 9 cut(s) 85, 150, 206, 250, 398, 711, 725, 1148, 1280
FaqI GGGAC 1 cut(s) 1024
FatI CATG 9 cut(s) 81, 146, 202, 246, 394, 707, 721, 1144, 1276
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 2 cut(s) 1020, 1118
FokI GGATG 1 cut(s) 471
FriOI GRGCYC 1 cut(s) 635
Fsp4HI GCNGC 2 cut(s) 1020, 1118
FspBI CTAG 2 cut(s) 1040, 1360
FspI TGCGCA 1 cut(s) 1143
GlaI GCGC 1 cut(s) 1143
GluI GCNGC 2 cut(s) 1020, 1118
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 2 cut(s) 360, 838
HapII CCGG 3 cut(s) 378, 648, 716
HgaI GACGC 1 cut(s) 1200
HhaI GCGC 1 cut(s) 1144
Hin1II CATG 9 cut(s) 85, 150, 206, 250, 398, 711, 725, 1148, 1280
Hin6I GCGC 1 cut(s) 1142
HinP1I GCGC 1 cut(s) 1142
HinfI GANTC 5 cut(s) 73, 508, 825, 1199, 1219
HpaII CCGG 3 cut(s) 378, 648, 716
HphI GGTGA 3 cut(s) 364, 662, 1114
Hpy188I TCNGA 7 cut(s) 270, 294, 492, 737, 763, 788, 1110
Hpy188III TCNNGA 4 cut(s) 432, 829, 929, 1075
Hpy99I CGWCG 2 cut(s) 989, 1258
HpyAV CCTTC 7 cut(s) 230, 309, 743, 808, 1239, 1335, 1342
HpyCH4III ACNGT 5 cut(s) 54, 332, 659, 1239, 1302
HpyCH4IV ACGT 5 cut(s) 673, 693, 702, 987, 992
HpyCH4V TGCA 9 cut(s) 38, 246, 572, 665, 795, 821, 1031, 1134, 1280
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 152, 1028, 1241, 1270
HpyF3I CTNAG 6 cut(s) 22, 129, 489, 762, 1269, 1336
HpySE526I ACGT 5 cut(s) 673, 693, 702, 987, 992
Hsp92II CATG 9 cut(s) 85, 150, 206, 250, 398, 711, 725, 1148, 1280
HspAI GCGC 1 cut(s) 1142
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 6 cut(s) 121, 176, 374, 492, 916, 998
LmnI GCTCC 3 cut(s) 295, 638, 1027
LweI GCATC 3 cut(s) 47, 449, 767
MaeI CTAG 2 cut(s) 1040, 1360
MaeII ACGT 5 cut(s) 673, 693, 702, 987, 992
MaeIII GTNAC 8 cut(s) 352, 694, 703, 988, 1046, 1064, 1212, 1352
MalI GATC 6 cut(s) 123, 178, 376, 494, 918, 1000
MbiI CCGCTC 2 cut(s) 1022, 1120
MboI GATC 6 cut(s) 121, 176, 374, 492, 916, 998
MboII GAAGA 6 cut(s) 307, 405, 545, 887, 1092, 1208
MfeI CAATTG 2 cut(s) 227, 282
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 635
MluCI AATT 9 cut(s) 33, 100, 227, 282, 561, 666, 676, 1136, 1321
MseI TTAA 3 cut(s) 485, 669, 690
MslI CAYNNNNRTG 1 cut(s) 1076
MspI CCGG 3 cut(s) 378, 648, 716
MspR9I CCNGG 2 cut(s) 717, 840
MunI CAATTG 2 cut(s) 227, 282
MvaI CCWGG 1 cut(s) 840
MwoI GCNNNNNNNGC 5 cut(s) 25, 152, 1028, 1241, 1270
NciI CCSGG 1 cut(s) 717
NcoI CCATGG 1 cut(s) 394
NdeII GATC 6 cut(s) 121, 176, 374, 492, 916, 998
NlaIII CATG 9 cut(s) 85, 150, 206, 250, 398, 711, 725, 1148, 1280
NlaIV GGNNCC 1 cut(s) 376
NmuCI GTSAC 6 cut(s) 352, 703, 988, 1046, 1064, 1212
NsbI TGCGCA 1 cut(s) 1143
NspI RCATGY 3 cut(s) 250, 711, 1148
PaeI GCATGC 1 cut(s) 250
PceI AGGCCT 1 cut(s) 360
PciI ACATGT 1 cut(s) 707
PcsI WCGNNNNNNNCGW 1 cut(s) 699
PfeI GAWTC 5 cut(s) 73, 508, 825, 1199, 1219
PinAI ACCGGT 1 cut(s) 647
PkrI GCNGC 2 cut(s) 1021, 1119
Ppu21I YACGTR 1 cut(s) 703
PpuMI RGGWCCY 1 cut(s) 239
PscI ACATGT 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 635
Psp5II RGGWCCY 1 cut(s) 239
Psp6I CCWGG 1 cut(s) 838
PspGI CCWGG 1 cut(s) 838
PspN4I GGNNCC 1 cut(s) 376
PspPI GGNCC 2 cut(s) 239, 713
PspPPI RGGWCCY 1 cut(s) 239
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 4 cut(s) 56, 771, 872, 1130
RsaNI GTAC 4 cut(s) 55, 770, 871, 1129
RseI CAYNNNNRTG 1 cut(s) 1076
SacI GAGCTC 1 cut(s) 635
SaqAI TTAA 3 cut(s) 485, 669, 690
SatI GCNGC 2 cut(s) 1020, 1118
Sau3AI GATC 6 cut(s) 121, 176, 374, 492, 916, 998
Sau96I GGNCC 2 cut(s) 239, 713
ScrFI CCNGG 2 cut(s) 717, 840
SduI GDGCHC 1 cut(s) 635
SfaNI GCATC 3 cut(s) 47, 449, 767
SfcI CTRYAG 1 cut(s) 1180
SinI GGWCC 2 cut(s) 239, 713
SmiMI CAYNNNNRTG 1 cut(s) 1076
SphI GCATGC 1 cut(s) 250
Sse9I AATT 9 cut(s) 33, 100, 227, 282, 561, 666, 676, 1136, 1321
SseBI AGGCCT 1 cut(s) 360
SsiI CCGC 4 cut(s) 1020, 1118, 1233, 1262
SspI AATATT 4 cut(s) 259, 625, 688, 865
SspMI CTAG 2 cut(s) 1040, 1360
SstI GAGCTC 1 cut(s) 635
StuI AGGCCT 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 715, 838
StyI CCWWGG 2 cut(s) 394, 503
TaaI ACNGT 5 cut(s) 54, 332, 659, 1239, 1302
TaiI ACGT 5 cut(s) 676, 696, 705, 990, 995
TaqI TCGA 3 cut(s) 67, 433, 915
TasI AATT 9 cut(s) 33, 100, 227, 282, 561, 666, 676, 1136, 1321
TatI WGTACW 1 cut(s) 769
TauI GCSGC 2 cut(s) 1022, 1120
TfiI GAWTC 5 cut(s) 73, 508, 825, 1199, 1219
Tru1I TTAA 3 cut(s) 485, 669, 690
Tru9I TTAA 3 cut(s) 485, 669, 690
TscAI CASTG 6 cut(s) 30, 376, 444, 808, 1221, 1305
TseFI GTSAC 6 cut(s) 352, 703, 988, 1046, 1064, 1212
Tsp45I GTSAC 6 cut(s) 352, 703, 988, 1046, 1064, 1212
TspDTI ATGAA 7 cut(s) 98, 219, 251, 545, 669, 1092, 1178
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 6 cut(s) 30, 376, 444, 808, 1221, 1305
VpaK11BI GGWCC 2 cut(s) 239, 713
XapI RAATTY 2 cut(s) 1136, 1321
XceI RCATGY 3 cut(s) 250, 711, 1148
XspI CTAG 2 cut(s) 1040, 1360
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.