pycom03g20830

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000579
Physical Location & Seq
Forward (+)
21245549 .. 21247036
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g20830.2

Sequence Viewer

Length: 1254 bp
ATGTCGATCACCAACACACTCGTTCACATTTTAATTTTCTTCTTCCTCGCTTTGTCGTTGTTTATTGGCATCGCACATAGCCAAAAAATACTGGACATAACCAAGTATGGTGCTGTGGGAGACGGCCGAATGGATGATTCCCCAGCTTTTGTGAATGCATGGAAAGATTTATGTCAAGGAATTAGTAAAGCTAGTAATGGCATACCAACACTTATTGTGCCTGAGGGGAAAACGTTCTTATTGCAACATATAAAATTTGAAGGCCCATGCAATACAAAGAACATTCACCTTCAAGTTCTAGGAAATTTGTTGGCACCTGCACGTGGTGCATGGAAAGAATGCGATTCGGAGGATTGGCTAAGTTTTTCAAATGTCGAAAACCTAATGCTTGATGGCTCAGGCAAAATCGACGGCCAGGGGGCTGCATGGTGGAGCACTAGCCAATACGACGGGAGATCACGCAAGCTCTTGGGGATAACGATTGCAAACCACAGAAGGCATTGCAATTCCAGAACTGCCAAAACCTCAGCTCAGCGGACTCACTTTTCTAAACAGCCCGCAAGTGCATATTGGATAGGTGACGATTGTATTTCCATTGGCGACGGGTGTTCCTTTCTCTCCATCACCAACATTGCTTGCGGACCAGGTCATGGAATTAGCAGTGTTGGAAGCTTGGGAGAAGATGGAGCTAGTAATAAAGTGGACAACATATATGTGAAAGATTGTAGTTTCACTGGAACTACAAACGGAGCAAGAATAAAGACGTGGCAAGGCGGTTCCGGGTATGCAAGGAACATCACGCTTGAGAGGATCAAACTTGACAGAGCTCAATATCCTATCATTATTGATCAGTACTATTGTAATGGGGAAAACAATTGCAAAAGTCAGTCGTCGGCCGTGGTGGTGGCGGATGTGACGTACATCGATTTTGAAGGCACTTCTGCGACGGAGGAGGCGATTAAACTCGATTGCGATCAAGTTTCAGGTGGCTGTCATAATATTGTGATGGATAGGATTAATATAATGCCAGCGGTGAGGGATATGAAGATTTTTGCGACTTGTAACAACACCAAAGGGACTTATGTCGGCACTACGGTGCCCAACGTGCATTGTCTACAGAGTTCAGCGACGGAGCCTACGAGGCCAACAACCATCTACACGGGAAGTGGAGCTTCTGGCAGTGCTAATCTACATATAAACTACTACCTGTTTTGTCCACTGATGACGGTTCTTGTTGCATCAGCTATTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

418

Amino Acids

45.16

Weight (kDa)

6.81

Isoelectric Point (pI)

38.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 1 cut(s) 325
AccB1I GGYRCC 2 cut(s) 313, 1096
AccB7I CCANNNNNTGG 1 cut(s) 650
AccI GTMKAC 1 cut(s) 1114
AciI CCGC 6 cut(s) 535, 558, 639, 774, 908, 1031
AclI AACGTT 1 cut(s) 233
AclWI GGATC 1 cut(s) 818
AcoI YGGCCR 3 cut(s) 124, 412, 894
AcsI RAATTY 2 cut(s) 254, 304
AcvI CACGTG 1 cut(s) 323
AdeI CACNNNGTG 1 cut(s) 326
AfaI GTAC 2 cut(s) 854, 920
AfiI CCNNNNNNNGG 2 cut(s) 323, 650
AgsI TTSAA 4 cut(s) 260, 293, 369, 932
AjiI CACGTC 1 cut(s) 765
AjnI CCWGG 2 cut(s) 414, 643
AleI CACNNNNGTG 1 cut(s) 1094
AluBI AGCT 9 cut(s) 146, 191, 466, 530, 672, 689, 827, 1172, 1244
AluI AGCT 9 cut(s) 146, 191, 466, 530, 672, 689, 827, 1172, 1244
Alw21I GWGCWC 2 cut(s) 437, 829
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 1 cut(s) 818
AoxI GGCC 5 cut(s) 124, 262, 412, 894, 1142
ApeKI GCWGC 1 cut(s) 422
ApoI RAATTY 2 cut(s) 254, 304
AseI ATTAAT 1 cut(s) 1017
Asp700I GAANNNNTTC 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 263, 641
AsuC2I CCSGG 1 cut(s) 781
AsuHPI GGTGA 4 cut(s) 278, 590, 616, 1045
AvaII GGWCC 1 cut(s) 641
AxyI CCTNAGG 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 1101
BanI GGYRCC 2 cut(s) 313, 1096
BanII GRGCYC 1 cut(s) 829
BbrPI CACGTG 1 cut(s) 323
Bbv12I GWGCWC 2 cut(s) 437, 829
BbvCI CCTCAGC 1 cut(s) 526
BbvI GCAGC 1 cut(s) 409
BccI CCATC 5 cut(s) 386, 629, 677, 1000, 1160
BceAI ACGGC 3 cut(s) 139, 427, 881
BciT130I CCWGG 2 cut(s) 416, 645
BclI TGATCA 1 cut(s) 847
BcnI CCSGG 1 cut(s) 781
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 4 cut(s) 192, 299, 438, 690
BfmI CTRYAG 1 cut(s) 1115
BfuAI ACCTGC 1 cut(s) 325
BglI GCCNNNNNGGC 1 cut(s) 1141
BisI GCNGC 1 cut(s) 423
BlpI GCTNAGC 1 cut(s) 531
BlsI GCNGC 1 cut(s) 424
BmcAI AGTACT 1 cut(s) 854
Bme1390I CCNGG 3 cut(s) 416, 645, 781
Bme18I GGWCC 1 cut(s) 641
BmgBI CACGTC 1 cut(s) 765
BmgT120I GGNCC 2 cut(s) 263, 641
BmiI GGNNCC 4 cut(s) 315, 778, 1098, 1134
BmrFI CCNGG 3 cut(s) 416, 645, 781
BmsI GCATC 2 cut(s) 78, 1247
BoxI GACNNNNGTC 1 cut(s) 1082
Bpu10I CCTNAGC 2 cut(s) 397, 526
Bpu1102I GCTNAGC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 824
BpuMI CCSGG 1 cut(s) 781
Bsa29I ATCGAT 1 cut(s) 924
BsaAI YACGTR 1 cut(s) 323
BsaBI GATNNNNATC 1 cut(s) 972
BsaJI CCNNGG 2 cut(s) 415, 897
Bsc4I CCNNNNNNNGG 2 cut(s) 323, 650
Bse1I ACTGG 2 cut(s) 96, 739
Bse21I CCTNAGG 1 cut(s) 222
Bse3DI GCAATG 2 cut(s) 499, 630
Bse8I GATNNNNATC 1 cut(s) 972
BseBI CCWGG 2 cut(s) 416, 645
BseCI ATCGAT 1 cut(s) 924
BseDI CCNNGG 2 cut(s) 415, 897
BseGI GGATG 2 cut(s) 139, 916
BseJI GATNNNNATC 1 cut(s) 972
BseLI CCNNNNNNNGG 2 cut(s) 323, 650
BseMI GCAATG 2 cut(s) 499, 630
BseMII CTCAG 4 cut(s) 213, 411, 540, 545
BseNI ACTGG 2 cut(s) 96, 739
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 1 cut(s) 1101
BseX3I CGGCCG 2 cut(s) 124, 894
BseXI GCAGC 1 cut(s) 409
BseYI CCCAGC 1 cut(s) 142
BsgI GTGCAG 1 cut(s) 303
Bsh1285I CGRYCG 2 cut(s) 127, 897
BshFI GGCC 5 cut(s) 126, 264, 414, 896, 1144
BshNI GGYRCC 2 cut(s) 313, 1096
BshVI ATCGAT 1 cut(s) 924
BsiEI CGRYCG 2 cut(s) 127, 897
BsiHKAI GWGCWC 2 cut(s) 437, 829
BsiSI CCGG 1 cut(s) 780
BslFI GGGAC 1 cut(s) 1090
BslI CCNNNNNNNGG 2 cut(s) 323, 650
BsmAI GTCTC 1 cut(s) 114
BsmBI CGTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 1090
BsmI GAATGC 2 cut(s) 160, 344
BsnI GGCC 5 cut(s) 126, 264, 414, 896, 1144
Bsp1286I GDGCHC 3 cut(s) 437, 829, 1101
Bsp143I GATC 5 cut(s) 6, 455, 810, 847, 973
Bsp1720I GCTNAGC 1 cut(s) 531
BspACI CCGC 6 cut(s) 535, 558, 639, 774, 908, 1031
BspANI GGCC 5 cut(s) 126, 264, 414, 896, 1144
BspCNI CTCAG 4 cut(s) 214, 410, 539, 544
BspDI ATCGAT 1 cut(s) 924
BspLI GGNNCC 4 cut(s) 315, 778, 1098, 1134
BspMI ACCTGC 1 cut(s) 325
BspPI GGATC 1 cut(s) 818
BspT107I GGYRCC 2 cut(s) 313, 1096
BsrDI GCAATG 2 cut(s) 499, 630
BsrI ACTGG 2 cut(s) 96, 739
BssECI CCNNGG 2 cut(s) 415, 897
BssMI GATC 5 cut(s) 6, 455, 810, 847, 973
Bst2UI CCWGG 2 cut(s) 416, 645
Bst4CI ACNGT 2 cut(s) 1096, 1228
BstAPI GCANNNNNTGC 1 cut(s) 326
BstBAI YACGTR 1 cut(s) 323
BstC8I GCNNGC 4 cut(s) 464, 558, 637, 1029
BstDEI CTNAG 5 cut(s) 222, 359, 397, 526, 531
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 2 cut(s) 139, 916
BstKTI GATC 5 cut(s) 9, 458, 813, 850, 976
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 5 cut(s) 6, 455, 810, 847, 973
BstMCI CGRYCG 2 cut(s) 127, 897
BstMWI GCNNNNNNNGC 3 cut(s) 326, 1105, 1141
BstNI CCWGG 2 cut(s) 416, 645
BstPAI GACNNNNGTC 1 cut(s) 1082
BstSCI CCNGG 3 cut(s) 414, 643, 779
BstSFI CTRYAG 1 cut(s) 1115
BstSLI GKGCMC 1 cut(s) 1101
BstV1I GCAGC 1 cut(s) 409
BstZI CGGCCG 2 cut(s) 124, 894
Bsu15I ATCGAT 1 cut(s) 924
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 5 cut(s) 126, 264, 414, 896, 1144
BsuTUI ATCGAT 1 cut(s) 924
BtgI CCRYGG 1 cut(s) 897
BtgZI GCGATG 1 cut(s) 55
BtrI CACGTC 1 cut(s) 765
BtsCI GGATG 2 cut(s) 139, 916
BtsI GCAGTG 2 cut(s) 667, 1186
BtsIMutI CAGTG 4 cut(s) 667, 732, 1186, 1217
BveI ACCTGC 1 cut(s) 325
Cac8I GCNNGC 4 cut(s) 464, 558, 637, 1029
Cfr13I GGNCC 2 cut(s) 263, 641
ClaI ATCGAT 1 cut(s) 924
CsiI ACCWGGT 1 cut(s) 643
Csp6I GTAC 2 cut(s) 853, 919
CviAII CATG 5 cut(s) 159, 267, 330, 426, 650
CviQI GTAC 2 cut(s) 853, 919
DdeI CTNAG 5 cut(s) 222, 359, 397, 526, 531
DpnI GATC 5 cut(s) 8, 457, 812, 849, 975
DpnII GATC 5 cut(s) 6, 455, 810, 847, 973
DraIII CACNNNGTG 1 cut(s) 326
EaeI YGGCCR 3 cut(s) 124, 412, 894
EagI CGGCCG 2 cut(s) 124, 894
EciI GGCGGA 1 cut(s) 923
Ecl136II GAGCTC 1 cut(s) 827
EclXI CGGCCG 2 cut(s) 124, 894
Eco24I GRGCYC 1 cut(s) 829
Eco47I GGWCC 1 cut(s) 641
Eco52I CGGCCG 2 cut(s) 124, 894
Eco53kI GAGCTC 1 cut(s) 827
Eco72I CACGTG 1 cut(s) 323
Eco81I CCTNAGG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 827
EcoRII CCWGG 2 cut(s) 414, 643
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 1 cut(s) 829
Esp3I CGTCTC 1 cut(s) 114
FaeI CATG 5 cut(s) 162, 270, 333, 429, 653
FalI AAGNNNNNCTT 2 cut(s) 1156, 1188
FaqI GGGAC 1 cut(s) 1090
FatI CATG 5 cut(s) 158, 266, 329, 425, 649
FauI CCCGC 1 cut(s) 565
FbaI TGATCA 1 cut(s) 847
FblI GTMKAC 1 cut(s) 1114
Fnu4HI GCNGC 1 cut(s) 423
FokI GGATG 2 cut(s) 146, 923
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 1 cut(s) 423
FspBI CTAG 4 cut(s) 192, 299, 438, 690
GluI GCNGC 1 cut(s) 423
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 5 cut(s) 126, 264, 414, 896, 1144
HapII CCGG 1 cut(s) 780
Hin1II CATG 5 cut(s) 162, 270, 333, 429, 653
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 3 cut(s) 137, 344, 538
HpaII CCGG 1 cut(s) 780
HphI GGTGA 4 cut(s) 278, 590, 616, 1045
Hpy166II GTNNAC 4 cut(s) 25, 703, 1115, 1217
Hpy188I TCNGA 1 cut(s) 349
Hpy188III TCNNGA 1 cut(s) 510
Hpy8I GTNNAC 4 cut(s) 25, 703, 1115, 1217
Hpy99I CGWCG 6 cut(s) 413, 452, 605, 895, 949, 1132
HpyAV CCTTC 4 cut(s) 254, 299, 489, 926
HpyCH4III ACNGT 2 cut(s) 1096, 1228
HpyCH4IV ACGT 5 cut(s) 233, 322, 764, 917, 1104
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 1105, 1141
HpyF3I CTNAG 5 cut(s) 222, 359, 397, 526, 531
HpySE526I ACGT 5 cut(s) 233, 322, 764, 917, 1104
Hsp92II CATG 5 cut(s) 162, 270, 333, 429, 653
Ksp22I TGATCA 1 cut(s) 847
Kzo9I GATC 5 cut(s) 6, 455, 810, 847, 973
LmnI GCTCC 5 cut(s) 432, 686, 749, 1132, 1169
Lsp1109I GCAGC 1 cut(s) 409
LweI GCATC 2 cut(s) 78, 1247
MabI ACCWGGT 1 cut(s) 643
MaeI CTAG 4 cut(s) 192, 299, 438, 690
MaeII ACGT 5 cut(s) 233, 322, 764, 917, 1104
MaeIII GTNAC 3 cut(s) 578, 913, 1061
MalI GATC 5 cut(s) 8, 457, 812, 849, 975
MboI GATC 5 cut(s) 6, 455, 810, 847, 973
MboII GAAGA 4 cut(s) 31, 34, 692, 1057
MfeI CAATTG 1 cut(s) 874
MhlI GDGCHC 3 cut(s) 437, 829, 1101
MluCI AATT 7 cut(s) 33, 180, 254, 304, 505, 654, 874
MlyI GAGTC 1 cut(s) 532
MmeI TCCRAC 1 cut(s) 646
MnlI CCTC 9 cut(s) 56, 217, 343, 535, 801, 943, 946, 1029, 1134
Mph1103I ATGCAT 1 cut(s) 160
MroXI GAANNNNTTC 1 cut(s) 233
MseI TTAA 3 cut(s) 32, 960, 1017
MslI CAYNNNNRTG 2 cut(s) 713, 1094
MspA1I CMGCKG 2 cut(s) 535, 1031
MspI CCGG 1 cut(s) 780
MspR9I CCNGG 3 cut(s) 416, 645, 781
MunI CAATTG 1 cut(s) 874
Mva1269I GAATGC 2 cut(s) 160, 344
MvaI CCWGG 2 cut(s) 416, 645
MwoI GCNNNNNNNGC 3 cut(s) 326, 1105, 1141
NciI CCSGG 1 cut(s) 781
NdeII GATC 5 cut(s) 6, 455, 810, 847, 973
NlaIII CATG 5 cut(s) 162, 270, 333, 429, 653
NlaIV GGNNCC 4 cut(s) 315, 778, 1098, 1134
NmuCI GTSAC 2 cut(s) 578, 913
NsiI ATGCAT 1 cut(s) 160
OliI CACNNNNGTG 1 cut(s) 1094
PaqCI CACCTGC 1 cut(s) 325
PcsI WCGNNNNNNNCGW 2 cut(s) 953, 1136
PctI GAATGC 2 cut(s) 160, 344
PdmI GAANNNNTTC 1 cut(s) 233
PfeI GAWTC 2 cut(s) 137, 344
PflFI GACNNNGTC 1 cut(s) 645
PflMI CCANNNNNTGG 1 cut(s) 650
PkrI GCNGC 1 cut(s) 424
PleI GAGTC 1 cut(s) 532
PmaCI CACGTG 1 cut(s) 323
PmlI CACGTG 1 cut(s) 323
PpsI GAGTC 1 cut(s) 532
Ppu21I YACGTR 1 cut(s) 323
PshAI GACNNNNGTC 1 cut(s) 1082
PshBI ATTAAT 1 cut(s) 1017
Psp124BI GAGCTC 1 cut(s) 829
Psp1406I AACGTT 1 cut(s) 233
Psp6I CCWGG 2 cut(s) 414, 643
PspCI CACGTG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 142
PspGI CCWGG 2 cut(s) 414, 643
PspN4I GGNNCC 4 cut(s) 315, 778, 1098, 1134
PspPI GGNCC 2 cut(s) 263, 641
PsyI GACNNNGTC 1 cut(s) 645
RsaI GTAC 2 cut(s) 854, 920
RsaNI GTAC 2 cut(s) 853, 919
RseI CAYNNNNRTG 2 cut(s) 713, 1094
SacI GAGCTC 1 cut(s) 829
SaqAI TTAA 3 cut(s) 32, 960, 1017
SatI GCNGC 1 cut(s) 423
Sau3AI GATC 5 cut(s) 6, 455, 810, 847, 973
Sau96I GGNCC 2 cut(s) 263, 641
ScaI AGTACT 1 cut(s) 854
SchI GAGTC 1 cut(s) 532
ScrFI CCNGG 3 cut(s) 416, 645, 781
SduI GDGCHC 3 cut(s) 437, 829, 1101
SexAI ACCWGGT 1 cut(s) 643
SfaNI GCATC 2 cut(s) 78, 1247
SfcI CTRYAG 1 cut(s) 1115
SinI GGWCC 1 cut(s) 641
SmiMI CAYNNNNRTG 2 cut(s) 713, 1094
SmlI CTYRAG 1 cut(s) 803
SmoI CTYRAG 1 cut(s) 803
Sse9I AATT 7 cut(s) 33, 180, 254, 304, 505, 654, 874
SsiI CCGC 6 cut(s) 535, 558, 639, 774, 908, 1031
SspI AATATT 1 cut(s) 1000
SspMI CTAG 4 cut(s) 192, 299, 438, 690
SstI GAGCTC 1 cut(s) 829
StyD4I CCNGG 3 cut(s) 414, 643, 779
TaaI ACNGT 2 cut(s) 1096, 1228
TaiI ACGT 5 cut(s) 236, 325, 767, 920, 1107
TaqI TCGA 5 cut(s) 5, 375, 408, 924, 966
TasI AATT 7 cut(s) 33, 180, 254, 304, 505, 654, 874
TatI WGTACW 1 cut(s) 852
TfiI GAWTC 2 cut(s) 137, 344
Tru1I TTAA 3 cut(s) 32, 960, 1017
Tru9I TTAA 3 cut(s) 32, 960, 1017
TscAI CASTG 4 cut(s) 667, 739, 1186, 1224
TseFI GTSAC 2 cut(s) 578, 913
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 2 cut(s) 578, 913
TspDTI ATGAA 1 cut(s) 1058
TspGWI ACGGA 3 cut(s) 762, 962, 1145
TspRI CASTG 4 cut(s) 667, 739, 1186, 1224
Tth111I GACNNNGTC 1 cut(s) 645
Van91I CCANNNNNTGG 1 cut(s) 650
VpaK11BI GGWCC 1 cut(s) 641
VspI ATTAAT 1 cut(s) 1017
XapI RAATTY 2 cut(s) 254, 304
XmiI GTMKAC 1 cut(s) 1114
XmnI GAANNNNTTC 1 cut(s) 233
XspI CTAG 4 cut(s) 192, 299, 438, 690
ZrmI AGTACT 1 cut(s) 854
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.