Rroxscaffold_153G00436780

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000153
OG0000579
Physical Location & Seq
Forward (+)
375784 .. 377362
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_153G00436780.1

Sequence Viewer

Length: 642 bp
ATGAGGAATATACTTGTCAACCTTTTGATGTTTTACTCTATTACTTCATCAAATTTTAGTTTTGGACATAGGCAAACAACTTTTGATGTGCTGGATTATGGTGCTGTTGGAGATGGCGATACCGATGATTCTGAAATTCTGGGGAAAATTGTGGCACCCAAAAATCCAGATGAATGGAAAGGGTGTGTAGAATCTTGGTTATCTTTCGGAAATCTGAGCAACCTCATAATAAATGGAACGGGAGAAATTAATGGCCAAGGTTCACTTTGGTGGAGCAATACCACCCAACCTAACAAAGCTCTACACTTTTTCAAATGTAGTAATCTTCAGTTAAACGGATTCGCACATATTGACAGTCCAAAGGCTCATATTAGCATCAACCGTAGCAATAATGTCACCATCTCTAATCTTCATATTTGTGCACCGGAAGATAGTCCTAACACAGATGGTATTGACATCTCTGTCTCAACACATGTCAATATTCATGACTCAACAATAGCAACTGGTAATCTTAATTCTCTTTCATGCATGCGTGAATCTACTAATCAACCTTATAAATCATTAACTATATGTGATTGTGGGAAGTTTAGGTGTGAATGGAAGTTATCAGACAGCAGAAGAGATACAAGTACGAAATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

213

Amino Acids

23.55

Weight (kDa)

5.75

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 57 - 169 1.3e-24 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 555
AasI GACNNNNNNGTC 1 cut(s) 461
AccB1I GGYRCC 1 cut(s) 154
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 52, 135
AcuI CTGAAG 1 cut(s) 311
AfaI GTAC 1 cut(s) 631
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 1 cut(s) 313
AleI CACNNNNGTG 1 cut(s) 268
AluBI AGCT 1 cut(s) 299
AluI AGCT 1 cut(s) 299
Alw21I GWGCWC 1 cut(s) 424
Alw26I GTCTC 1 cut(s) 469
Alw44I GTGCAC 1 cut(s) 420
AoxI GGCC 1 cut(s) 253
ApaLI GTGCAC 1 cut(s) 420
ApoI RAATTY 2 cut(s) 52, 135
AseI ATTAAT 1 cut(s) 249
AsuHPI GGTGA 1 cut(s) 388
BaeGI GKGCMC 1 cut(s) 424
BalI TGGCCA 1 cut(s) 255
BanI GGYRCC 1 cut(s) 154
Bbv12I GWGCWC 1 cut(s) 424
BccI CCATC 3 cut(s) 107, 407, 440
BcoDI GTCTC 1 cut(s) 469
BmiI GGNNCC 1 cut(s) 156
BmsI GCATC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 256
BsaWI WCCGGW 1 cut(s) 424
Bse1I ACTGG 1 cut(s) 508
BseDI CCNNGG 1 cut(s) 256
BseMII CTCAG 1 cut(s) 206
BseNI ACTGG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 424
BshFI GGCC 1 cut(s) 255
BshNI GGYRCC 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 424
BsiSI CCGG 1 cut(s) 425
BsmAI GTCTC 1 cut(s) 469
BsnI GGCC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 424
BspANI GGCC 1 cut(s) 255
BspCNI CTCAG 1 cut(s) 207
BspHI TCATGA 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 156
BspT107I GGYRCC 1 cut(s) 154
BsrI ACTGG 1 cut(s) 508
BssECI CCNNGG 1 cut(s) 256
BssT1I CCWWGG 1 cut(s) 256
Bst4CI ACNGT 2 cut(s) 356, 383
Bst6I CTCTTC 1 cut(s) 613
BstC8I GCNNGC 1 cut(s) 530
BstDEI CTNAG 1 cut(s) 215
BstMAI GTCTC 1 cut(s) 469
BstNSI RCATGY 2 cut(s) 476, 532
BstSLI GKGCMC 1 cut(s) 424
BstXI CCANNNNNNTGG 1 cut(s) 174
BsuRI GGCC 1 cut(s) 255
Cac8I GCNNGC 1 cut(s) 530
CciI TCATGA 1 cut(s) 484
Csp6I GTAC 1 cut(s) 630
CviAII CATG 4 cut(s) 473, 485, 525, 529
CviJI RGCY 3 cut(s) 255, 299, 365
CviKI_1 RGCY 3 cut(s) 255, 299, 365
CviQI GTAC 1 cut(s) 630
DdeI CTNAG 1 cut(s) 215
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 1 cut(s) 253
Eam1104I CTCTTC 1 cut(s) 613
EarI CTCTTC 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 256
Eco57I CTGAAG 1 cut(s) 311
EcoT14I CCWWGG 1 cut(s) 256
EcoT22I ATGCAT 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 256
FaeI CATG 4 cut(s) 476, 488, 528, 532
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FatI CATG 4 cut(s) 472, 484, 524, 528
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 425
Hin1II CATG 4 cut(s) 476, 488, 528, 532
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HinfI GANTC 5 cut(s) 128, 191, 339, 488, 536
HpaII CCGG 1 cut(s) 425
HphI GGTGA 1 cut(s) 388
Hpy166II GTNNAC 3 cut(s) 19, 263, 422
Hpy188I TCNGA 4 cut(s) 133, 209, 216, 610
Hpy188III TCNNGA 2 cut(s) 167, 485
Hpy8I GTNNAC 3 cut(s) 19, 263, 422
HpyCH4III ACNGT 2 cut(s) 356, 383
HpyCH4V TGCA 2 cut(s) 422, 528
HpyF3I CTNAG 1 cut(s) 215
Hsp92II CATG 4 cut(s) 476, 488, 528, 532
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 5 cut(s) 77, 125, 180, 438, 489
LweI GCATC 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 394
MboII GAAGA 4 cut(s) 317, 401, 440, 630
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 255
MluCI AATT 6 cut(s) 52, 135, 147, 246, 514, 635
MluNI TGGCCA 1 cut(s) 255
MlyI GAGTC 1 cut(s) 482
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 1 cut(s) 233
Mox20I TGGCCA 1 cut(s) 255
Mph1103I ATGCAT 1 cut(s) 530
MscI TGGCCA 1 cut(s) 255
MseI TTAA 4 cut(s) 249, 332, 513, 563
MslI CAYNNNNRTG 2 cut(s) 268, 417
Msp20I TGGCCA 1 cut(s) 255
MspI CCGG 1 cut(s) 425
NlaIII CATG 4 cut(s) 476, 488, 528, 532
NlaIV GGNNCC 1 cut(s) 156
NmuCI GTSAC 1 cut(s) 394
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 2 cut(s) 476, 532
OliI CACNNNNGTG 1 cut(s) 268
PaeI GCATGC 1 cut(s) 532
PagI TCATGA 1 cut(s) 484
PciI ACATGT 1 cut(s) 472
PfeI GAWTC 4 cut(s) 128, 191, 339, 536
PleI GAGTC 1 cut(s) 482
PpsI GAGTC 1 cut(s) 482
PscI ACATGT 1 cut(s) 472
PshBI ATTAAT 1 cut(s) 249
PsiI TTATAA 1 cut(s) 555
PspN4I GGNNCC 1 cut(s) 156
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
RseI CAYNNNNRTG 2 cut(s) 268, 417
SaqAI TTAA 4 cut(s) 249, 332, 513, 563
SchI GAGTC 1 cut(s) 482
SduI GDGCHC 1 cut(s) 424
SetI ASST 7 cut(s) 24, 225, 262, 292, 301, 553, 593
SfaNI GCATC 1 cut(s) 384
SmiMI CAYNNNNRTG 2 cut(s) 268, 417
SphI GCATGC 1 cut(s) 532
Sse9I AATT 6 cut(s) 52, 135, 147, 246, 514, 635
SspI AATATT 1 cut(s) 481
StyI CCWWGG 1 cut(s) 256
TaaI ACNGT 2 cut(s) 356, 383
TasI AATT 6 cut(s) 52, 135, 147, 246, 514, 635
TfiI GAWTC 4 cut(s) 128, 191, 339, 536
Tru1I TTAA 4 cut(s) 249, 332, 513, 563
Tru9I TTAA 4 cut(s) 249, 332, 513, 563
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 5 cut(s) 36, 186, 401, 473, 513
TspGWI ACGGA 1 cut(s) 351
VneI GTGCAC 1 cut(s) 420
VspI ATTAAT 1 cut(s) 249
XapI RAATTY 2 cut(s) 52, 135
XceI RCATGY 2 cut(s) 476, 532
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.