Rw4G007770

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000579
Physical Location & Seq
Forward (+)
16737302 .. 16741755
4454 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G007770.1

Sequence Viewer

Length: 1356 bp
ATGTTTCCAACTATATTAATAACATTTTCCCTTAATTTTTCTTTTTTACAGGGTCTACTTTCGCTACTTACCTCTGTGTTGTTTTCATGGATTGTTGCTTCACCTAGATTTGGCATTGGGCCGCGTTATGGCCAAAATCGTACTGTTAACGTACTTGATTTTGGTGCCTCTGGTGATGGCATAGCAGATGATACTGAAGCTTTCTTAAATGCATGGAAGACACTTTGCGAGACTGGCCAAACCCTAAACATCCCCGCAGGGAAGACCTTTGTAGTCAAACCTCTTCAACTTGTAGGTCCATGCAAATCCAATAACGTTGGAATTCAGATTGATGGGAATATTGTCGGTCCATCCACTCTTAGCGAATGGGCAGACGCGTGCAAAATCGGACAATGTTGGCTATGCTTTCTAAACGTGAAGGGCCTCGTCATCAAGGGGGAAGGACTTATAAATGGCAATGGTCCAATTTGGTGGAAACAAGAAACCCAATCCATAAATGCTTTAAACTTTCACAAATGCGACCAACTTCAATTATCTGGATTTACTTCCCGTGATAGTCCGAGAGCCCATATATTTATACATAGCAGCAATGGTGTACGTATCTCAAACATTCACTTGAGTGCACCGGAAAAAAGCCCGAATACTGATGGCATTGACATCTCTTTATCAAGCCAAGTGACCATACAAGACTCTTTCATCGGAACTGGTGATGATTGCATTGCAATTAAGGGAGGCTCCTCTTTTGTAAACATAACTCATGTAACATGCGGACCAGGTCATGGCTTCAGTGTGGGTAGTTTAGGACAAGATCCAGCTATTACAGATAATGTCGAACAAGTACACTTTCAGAATTGTATCTGCAACAAATCTACAAACTGTGCAAGAATCAAAACATGGATGGGTGGATCTGGATACGCTCGGGCGATTTTCTTCAGACAAATGATCCTTGAACAGTCACAAAATCCTATTATAATTGACCAACACTACTGCAATGGCGGACATAATTGCCCAGCTAAGGTAAAAACGGGAGCAGTGAAAGTTAGCAATGTAACATTCAGTGGCATTACCGGAACTTCAGCAAGTGAGCAAGCAATAACACTGGATTGCTCCGACTTGGGTTGTACCGATATCTTGATGGATCATGTCAACATAACCTCCGCATCCGGACTACCACTTCATTCTGTGTGCAACAAGGCTAGTGGGAAAGCTAGTTTTACTTCACCTCCGATTCCTTGCCTGTCGTCGAATGGATCTTTGTCATCAGTGATTGCACCGATCTCGGTCAACTTATTAAAGCCAAACCATTCTTATTTGAGTTATTTCGGAATTCATGCCCATAAAACAATCGAAGCTTAA

Protein Analysis

451

Amino Acids

48.12

Weight (kDa)

6.89

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 49 - 270 2e-11 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 80 - 400 2.5e-77 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 449, 971
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 1 cut(s) 779
AccI GTMKAC 1 cut(s) 55
AccII CGCG 2 cut(s) 124, 377
AccIII TCCGGA 1 cut(s) 1163
AciI CCGC 5 cut(s) 122, 255, 768, 996, 1158
AclI AACGTT 1 cut(s) 315
AclWI GGATC 5 cut(s) 803, 913, 937, 1146, 1258
AcoI YGGCCR 2 cut(s) 130, 235
AcsI RAATTY 2 cut(s) 321, 1326
AcuI CTGAAG 4 cut(s) 216, 769, 916, 1059
AfaI GTAC 5 cut(s) 142, 153, 597, 840, 1123
AfiI CCNNNNNNNGG 4 cut(s) 110, 128, 779, 1015
AflIII ACRYGT 1 cut(s) 375
AgsI TTSAA 3 cut(s) 287, 530, 950
AjnI CCWGG 1 cut(s) 772
AleI CACNNNNGTG 1 cut(s) 618
AluBI AGCT 5 cut(s) 200, 815, 1013, 1208, 1352
AluI AGCT 5 cut(s) 200, 815, 1013, 1208, 1352
Alw21I GWGCWC 1 cut(s) 625
Alw26I GTCTC 1 cut(s) 224
Alw44I GTGCAC 1 cut(s) 621
AlwI GGATC 5 cut(s) 803, 913, 937, 1146, 1258
Ama87I CYCGRG 1 cut(s) 918
Aor13HI TCCGGA 1 cut(s) 1163
AoxI GGCC 4 cut(s) 119, 130, 235, 421
ApaLI GTGCAC 1 cut(s) 621
ApeKI GCWGC 1 cut(s) 585
ApoI RAATTY 2 cut(s) 321, 1326
AseI ATTAAT 1 cut(s) 17
AspS9I GGNCC 6 cut(s) 119, 296, 347, 421, 461, 770
AsuHPI GGTGA 4 cut(s) 93, 185, 719, 1212
AvaI CYCGRG 1 cut(s) 918
AvaII GGWCC 4 cut(s) 296, 347, 461, 770
BaeGI GKGCMC 1 cut(s) 625
BalI TGGCCA 2 cut(s) 132, 237
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 568
BbsI GAAGAC 2 cut(s) 224, 269
Bbv12I GWGCWC 1 cut(s) 625
BbvI GCAGC 1 cut(s) 597
BccI CCATC 6 cut(s) 170, 326, 358, 641, 892, 1129
BcgI CGANNNNNNTGC 2 cut(s) 1260, 1294
BciT130I CCWGG 1 cut(s) 774
BciVI GTATCC 1 cut(s) 905
BcoDI GTCTC 1 cut(s) 224
BfaI CTAG 3 cut(s) 105, 1197, 1209
BfuI GTATCC 1 cut(s) 905
BisI GCNGC 2 cut(s) 122, 586
BlsI GCNGC 2 cut(s) 123, 587
Bme1390I CCNGG 1 cut(s) 774
Bme18I GGWCC 4 cut(s) 296, 347, 461, 770
BmeT110I CYCGRG 1 cut(s) 918
BmgT120I GGNCC 6 cut(s) 119, 296, 347, 421, 461, 770
BmiI GGNNCC 2 cut(s) 166, 736
BmrFI CCNGG 1 cut(s) 774
BmsI GCATC 1 cut(s) 1169
BpiI GAAGAC 2 cut(s) 224, 269
Bpu10I CCTNAGC 1 cut(s) 1014
BpuEI CTTGAG 1 cut(s) 637
BsaAI YACGTR 1 cut(s) 599
BsaWI WCCGGW 3 cut(s) 625, 1067, 1163
BsaXI ACNNNNNCTCC 4 cut(s) 1139, 1169, 1207, 1237
Bsc4I CCNNNNNNNGG 4 cut(s) 110, 128, 779, 1015
Bse1I ACTGG 3 cut(s) 238, 709, 1104
Bse3DI GCAATG 5 cut(s) 463, 595, 717, 997, 1051
BseAI TCCGGA 1 cut(s) 1163
BseBI CCWGG 1 cut(s) 774
BseGI GGATG 4 cut(s) 249, 350, 903, 1160
BseLI CCNNNNNNNGG 4 cut(s) 110, 128, 779, 1015
BseMI GCAATG 5 cut(s) 463, 595, 717, 997, 1051
BseNI ACTGG 3 cut(s) 238, 709, 1104
BseRI GAGGAG 1 cut(s) 727
BseSI GKGCMC 1 cut(s) 625
BseXI GCAGC 1 cut(s) 597
BseYI CCCAGC 1 cut(s) 1009
Bsh1236I CGCG 2 cut(s) 124, 377
BshFI GGCC 4 cut(s) 121, 132, 237, 423
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 625
BsiHKCI CYCGRG 1 cut(s) 918
BsiSI CCGG 3 cut(s) 626, 1068, 1164
BslI CCNNNNNNNGG 4 cut(s) 110, 128, 779, 1015
BsmAI GTCTC 1 cut(s) 224
BsnI GGCC 4 cut(s) 121, 132, 237, 423
BsoBI CYCGRG 1 cut(s) 918
Bsp1286I GDGCHC 2 cut(s) 568, 625
Bsp13I TCCGGA 1 cut(s) 1163
Bsp143I GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
BspACI CCGC 5 cut(s) 122, 255, 768, 996, 1158
BspANI GGCC 4 cut(s) 121, 132, 237, 423
BspEI TCCGGA 1 cut(s) 1163
BspFNI CGCG 2 cut(s) 124, 377
BspLI GGNNCC 2 cut(s) 166, 736
BspPI GGATC 5 cut(s) 803, 913, 937, 1146, 1258
BspT107I GGYRCC 1 cut(s) 164
BsrDI GCAATG 5 cut(s) 463, 595, 717, 997, 1051
BsrI ACTGG 3 cut(s) 238, 709, 1104
BssMI GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
Bst2UI CCWGG 1 cut(s) 774
Bst4CI ACNGT 3 cut(s) 145, 878, 954
Bst6I CTCTTC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 599
BstC8I GCNNGC 2 cut(s) 379, 1089
BstDEI CTNAG 2 cut(s) 359, 1014
BstF5I GGATG 4 cut(s) 249, 350, 903, 1160
BstFNI CGCG 2 cut(s) 124, 377
BstKTI GATC 6 cut(s) 811, 908, 945, 1141, 1253, 1278
BstMAI GTCTC 1 cut(s) 224
BstMBI GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 774
BstNSI RCATGY 1 cut(s) 768
BstSCI CCNGG 1 cut(s) 772
BstSLI GKGCMC 1 cut(s) 625
BstSNI TACGTA 1 cut(s) 599
BstUI CGCG 2 cut(s) 124, 377
BstV1I GCAGC 1 cut(s) 597
BstV2I GAAGAC 2 cut(s) 224, 269
BstX2I RGATCY 3 cut(s) 808, 905, 1250
BstXI CCANNNNNNTGG 1 cut(s) 471
BstYI RGATCY 3 cut(s) 808, 905, 1250
BsuI GTATCC 1 cut(s) 905
BsuRI GGCC 4 cut(s) 121, 132, 237, 423
BtsCI GGATG 4 cut(s) 249, 350, 903, 1160
BtsI GCAGTG 1 cut(s) 1038
BtsIMutI CAGTG 5 cut(s) 793, 1038, 1063, 1097, 1269
Cac8I GCNNGC 2 cut(s) 379, 1089
Cfr13I GGNCC 6 cut(s) 119, 296, 347, 421, 461, 770
CseI GACGC 1 cut(s) 383
CsiI ACCWGGT 1 cut(s) 772
Csp6I GTAC 5 cut(s) 141, 152, 596, 839, 1122
CspCI CAANNNNNGTGG 2 cut(s) 1180, 1215
CviAII CATG 9 cut(s) 87, 213, 300, 758, 765, 779, 894, 1142, 1331
CviQI GTAC 5 cut(s) 141, 152, 596, 839, 1122
DdeI CTNAG 2 cut(s) 359, 1014
DpnI GATC 6 cut(s) 810, 907, 944, 1140, 1252, 1277
DpnII GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
DraI TTTAAA 1 cut(s) 504
EaeI YGGCCR 2 cut(s) 130, 235
Eam1104I CTCTTC 1 cut(s) 288
EarI CTCTTC 1 cut(s) 288
EciI GGCGGA 1 cut(s) 1011
Eco105I TACGTA 1 cut(s) 599
Eco24I GRGCYC 1 cut(s) 568
Eco32I GATATC 1 cut(s) 1129
Eco47I GGWCC 4 cut(s) 296, 347, 461, 770
Eco57I CTGAAG 4 cut(s) 216, 769, 916, 1059
Eco88I CYCGRG 1 cut(s) 918
EcoO109I RGGNCCY 1 cut(s) 421
EcoRI GAATTC 2 cut(s) 321, 1326
EcoRII CCWGG 1 cut(s) 772
EcoRV GATATC 1 cut(s) 1129
EcoT22I ATGCAT 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 568
FaeI CATG 9 cut(s) 90, 216, 303, 761, 768, 782, 897, 1145, 1334
FatI CATG 9 cut(s) 86, 212, 299, 757, 764, 778, 893, 1141, 1330
FauI CCCGC 1 cut(s) 262
FblI GTMKAC 1 cut(s) 55
Fnu4HI GCNGC 2 cut(s) 122, 586
FokI GGATG 4 cut(s) 236, 337, 910, 1147
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 2 cut(s) 122, 586
FspBI CTAG 3 cut(s) 105, 1197, 1209
GluI GCNGC 2 cut(s) 122, 586
GsaI CCCAGC 1 cut(s) 1013
HaeIII GGCC 4 cut(s) 121, 132, 237, 423
HapII CCGG 3 cut(s) 626, 1068, 1164
HgaI GACGC 1 cut(s) 383
Hin1II CATG 9 cut(s) 90, 216, 303, 761, 768, 782, 897, 1145, 1334
HincII GTYRAC 3 cut(s) 148, 1147, 1285
HindII GTYRAC 3 cut(s) 148, 1147, 1285
HindIII AAGCTT 2 cut(s) 198, 1350
HinfI GANTC 3 cut(s) 689, 885, 1228
HpaI GTTAAC 1 cut(s) 148
HpaII CCGG 3 cut(s) 626, 1068, 1164
HphI GGTGA 4 cut(s) 93, 185, 719, 1212
Hpy166II GTNNAC 8 cut(s) 56, 148, 596, 623, 748, 841, 1147, 1285
Hpy188I TCNGA 9 cut(s) 327, 389, 561, 701, 849, 935, 1111, 1227, 1325
Hpy188III TCNNGA 4 cut(s) 537, 909, 1132, 1164
Hpy8I GTNNAC 8 cut(s) 56, 148, 596, 623, 748, 841, 1147, 1285
Hpy99I CGWCG 1 cut(s) 1246
HpyAV CCTTC 2 cut(s) 412, 434
HpyCH4III ACNGT 3 cut(s) 145, 878, 954
HpyCH4IV ACGT 4 cut(s) 150, 315, 414, 598
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 2 cut(s) 359, 1014
HpySE526I ACGT 4 cut(s) 150, 315, 414, 598
Hsp92II CATG 9 cut(s) 90, 216, 303, 761, 768, 782, 897, 1145, 1334
Kpn2I TCCGGA 1 cut(s) 1163
KspAI GTTAAC 1 cut(s) 148
Kzo9I GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
LmnI GCTCC 3 cut(s) 740, 1028, 1112
Lsp1109I GCAGC 1 cut(s) 597
LweI GCATC 1 cut(s) 1169
MabI ACCWGGT 1 cut(s) 772
MaeI CTAG 3 cut(s) 105, 1197, 1209
MaeII ACGT 4 cut(s) 150, 315, 414, 598
MaeIII GTNAC 4 cut(s) 676, 760, 954, 1048
MalI GATC 6 cut(s) 810, 907, 944, 1140, 1252, 1277
MboI GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
MboII GAAGA 4 cut(s) 229, 274, 275, 922
MflI RGATCY 3 cut(s) 808, 905, 1250
MhlI GDGCHC 2 cut(s) 568, 625
MlsI TGGCCA 2 cut(s) 132, 237
MluCI AATT 9 cut(s) 34, 321, 465, 530, 723, 850, 972, 1003, 1326
MluI ACGCGT 1 cut(s) 375
MluNI TGGCCA 2 cut(s) 132, 237
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 3 cut(s) 32, 298, 1134
MnlI CCTC 8 cut(s) 82, 178, 291, 434, 725, 748, 1165, 1233
Mox20I TGGCCA 2 cut(s) 132, 237
Mph1103I ATGCAT 1 cut(s) 214
MroI TCCGGA 1 cut(s) 1163
MscI TGGCCA 2 cut(s) 132, 237
MseI TTAA 8 cut(s) 17, 33, 147, 206, 503, 726, 1292, 1354
MslI CAYNNNNRTG 1 cut(s) 618
Msp20I TGGCCA 2 cut(s) 132, 237
MspI CCGG 3 cut(s) 626, 1068, 1164
MspR9I CCNGG 1 cut(s) 774
MvaI CCWGG 1 cut(s) 774
MvnI CGCG 2 cut(s) 124, 377
MwoI GCNNNNNNNGC 1 cut(s) 234
NdeII GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
NlaIII CATG 9 cut(s) 90, 216, 303, 761, 768, 782, 897, 1145, 1334
NlaIV GGNNCC 2 cut(s) 166, 736
NmuCI GTSAC 2 cut(s) 676, 954
NsiI ATGCAT 1 cut(s) 214
NspI RCATGY 1 cut(s) 768
OliI CACNNNNGTG 1 cut(s) 618
PfeI GAWTC 2 cut(s) 885, 1228
PflFI GACNNNGTC 1 cut(s) 774
PflMI CCANNNNNTGG 1 cut(s) 779
PkrI GCNGC 2 cut(s) 123, 587
PleI GAGTC 1 cut(s) 683
PpsI GAGTC 1 cut(s) 683
Ppu21I YACGTR 1 cut(s) 599
PshBI ATTAAT 1 cut(s) 17
PsiI TTATAA 2 cut(s) 449, 971
Psp1406I AACGTT 1 cut(s) 315
Psp6I CCWGG 1 cut(s) 772
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 1 cut(s) 772
PspN4I GGNNCC 2 cut(s) 166, 736
PspPI GGNCC 6 cut(s) 119, 296, 347, 421, 461, 770
PsuI RGATCY 3 cut(s) 808, 905, 1250
PsyI GACNNNGTC 1 cut(s) 774
RsaI GTAC 5 cut(s) 142, 153, 597, 840, 1123
RsaNI GTAC 5 cut(s) 141, 152, 596, 839, 1122
RseI CAYNNNNRTG 1 cut(s) 618
SaqAI TTAA 8 cut(s) 17, 33, 147, 206, 503, 726, 1292, 1354
SatI GCNGC 2 cut(s) 122, 586
Sau3AI GATC 6 cut(s) 808, 905, 942, 1138, 1250, 1275
Sau96I GGNCC 6 cut(s) 119, 296, 347, 421, 461, 770
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 1 cut(s) 774
SduI GDGCHC 2 cut(s) 568, 625
SexAI ACCWGGT 1 cut(s) 772
SfaNI GCATC 1 cut(s) 1169
SinI GGWCC 4 cut(s) 296, 347, 461, 770
SmiMI CAYNNNNRTG 1 cut(s) 618
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SnaBI TACGTA 1 cut(s) 599
Sse9I AATT 9 cut(s) 34, 321, 465, 530, 723, 850, 972, 1003, 1326
SsiI CCGC 5 cut(s) 122, 255, 768, 996, 1158
SspI AATATT 1 cut(s) 340
SspMI CTAG 3 cut(s) 105, 1197, 1209
StyD4I CCNGG 1 cut(s) 772
TaaI ACNGT 3 cut(s) 145, 878, 954
TaiI ACGT 4 cut(s) 153, 318, 417, 601
TaqI TCGA 3 cut(s) 831, 1244, 1347
TaqII GACCGA 2 cut(s) 335, 1270
TasI AATT 9 cut(s) 34, 321, 465, 530, 723, 850, 972, 1003, 1326
TatI WGTACW 1 cut(s) 838
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 2 cut(s) 885, 1228
Tru1I TTAA 8 cut(s) 17, 33, 147, 206, 503, 726, 1292, 1354
Tru9I TTAA 8 cut(s) 17, 33, 147, 206, 503, 726, 1292, 1354
TscAI CASTG 5 cut(s) 793, 1038, 1063, 1104, 1269
TseFI GTSAC 2 cut(s) 676, 954
TseI GCWGC 1 cut(s) 585
Tsp45I GTSAC 2 cut(s) 676, 954
TspDTI ATGAA 4 cut(s) 75, 685, 1166, 1319
TspRI CASTG 5 cut(s) 793, 1038, 1063, 1104, 1269
Tth111I GACNNNGTC 1 cut(s) 774
Van91I CCANNNNNTGG 1 cut(s) 779
VneI GTGCAC 1 cut(s) 621
VpaK11BI GGWCC 4 cut(s) 296, 347, 461, 770
VspI ATTAAT 1 cut(s) 17
XapI RAATTY 2 cut(s) 321, 1326
XceI RCATGY 1 cut(s) 768
XmiI GTMKAC 1 cut(s) 55
XspI CTAG 3 cut(s) 105, 1197, 1209
Zsp2I ATGCAT 1 cut(s) 214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.