Rmu_sc0002800.1_g000012

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002800.1
OG0000579
Physical Location & Seq
Reverse (-)
29240 .. 32545
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002800.1_g000012.1.cds

Sequence Viewer

Length: 957 bp
atggctgctgacatagatccggtgaaggaggatgttgtgaggggcgcgtgggacagtggtggtgttttggccgttgtccatcgtggctggctggctgacagaggtggcaaaatgaggcaatttgatttatctaggtctagtggttcgctcttcatcttgagttttacatcacttgggggcggtcggtgtgggattgagtcagcggtggttctggcgatgggaatggaatgggagtctgttgcttctaacccagagccaattgtgtatctagatcgattcggatctgggattcgggtggaatctacaacaggatccagatctgggatccaggctctgcagttcgacaattgtgaccaacttaccctagatggatttacttctattgacagtccaagagttcatatcacaatgcacaaatgtactggtatccaagtctcaaacgttcatataattaagtgcacctgcaactctgcaagcagccccaacaccgatggcttcggtattgttgggtcaacacaagtaaatataagggattctgtcattgcaactggagatgactgcattgcaattaagggaggttgctcttttataaacataaccggtgtgcaatgcggaccaggtcatggtatcaggtatgggcacgctagagcgatatcttttgagcaaatcacgcttgaaaatgcaaagaatcccattataattaagcaaacgtacgttgataaaaatggagcagtgaaggtgagtgatgtaacatttcgcagctttgaaggaacaccagcttccgcgcaagcaataacattgaactgctcagagttgggctgcaccaacattgtaatggatcaaatcgccttaagctcggccgtgcccggaaagtcattgcattcttattgcagaaatgcccaaggaagatcaatccttacttcaccttatgttccttgtctagaacagtcaaaatga

Protein Analysis

318

Amino Acids

33.77

Weight (kDa)

6.38

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 590, 698
AarI CACCTGC 1 cut(s) 470
Acc36I ACCTGC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 623
AccII CGCG 2 cut(s) 47, 785
AciI CCGC 4 cut(s) 180, 203, 612, 783
AclI AACGTT 1 cut(s) 441
AclWI GGATC 7 cut(s) 11, 289, 306, 319, 319, 332, 846
AcoI YGGCCR 2 cut(s) 69, 858
AfaI GTAC 2 cut(s) 421, 713
AfiI CCNNNNNNNGG 2 cut(s) 321, 623
AflII CTTAAG 1 cut(s) 850
AgeI ACCGGT 1 cut(s) 599
AgsI TTSAA 3 cut(s) 677, 767, 802
AjnI CCWGG 2 cut(s) 327, 616
AluBI AGCT 3 cut(s) 762, 779, 855
AluI AGCT 3 cut(s) 762, 779, 855
Alw21I GWGCWC 1 cut(s) 461
Alw26I GTCTC 1 cut(s) 439
Alw44I GTGCAC 1 cut(s) 457
AlwI GGATC 7 cut(s) 11, 289, 306, 319, 319, 332, 846
AlwNI CAGNNNCTG 1 cut(s) 334
AoxI GGCC 2 cut(s) 69, 858
ApaLI GTGCAC 1 cut(s) 457
ApeKI GCWGC 4 cut(s) 5, 477, 759, 819
AsiGI ACCGGT 1 cut(s) 599
AspLEI GCGC 2 cut(s) 47, 787
AspS9I GGNCC 1 cut(s) 614
AsuC2I CCSGG 1 cut(s) 867
AsuHPI GGTGA 3 cut(s) 34, 751, 915
AvaII GGWCC 1 cut(s) 614
BaeGI GKGCMC 3 cut(s) 461, 642, 867
BamHI GGATCC 2 cut(s) 311, 324
Bbv12I GWGCWC 1 cut(s) 461
BbvI GCAGC 3 cut(s) 489, 771, 806
BccI CCATC 4 cut(s) 87, 211, 362, 485
BceAI ACGGC 2 cut(s) 56, 845
BciT130I CCWGG 2 cut(s) 329, 618
BciVI GTATCC 1 cut(s) 437
BcnI CCSGG 1 cut(s) 867
BcoDI GTCTC 1 cut(s) 439
BfaI CTAG 6 cut(s) 132, 138, 269, 365, 645, 941
BfmI CTRYAG 1 cut(s) 335
BfrI CTTAAG 1 cut(s) 850
BfuAI ACCTGC 1 cut(s) 470
BfuI GTATCC 1 cut(s) 437
BglII AGATCT 1 cut(s) 317
BisI GCNGC 4 cut(s) 6, 478, 760, 820
BlsI GCNGC 4 cut(s) 7, 479, 761, 821
Bme1390I CCNGG 3 cut(s) 329, 618, 867
Bme18I GGWCC 1 cut(s) 614
BmgT120I GGNCC 1 cut(s) 614
BmiI GGNNCC 2 cut(s) 313, 326
BmrFI CCNGG 3 cut(s) 329, 618, 867
BpmI CTGGAG 1 cut(s) 570
BpuEI CTTGAG 1 cut(s) 178
BpuMI CCSGG 1 cut(s) 867
Bsa29I ATCGAT 1 cut(s) 274
BsaBI GATNNNNATC 2 cut(s) 280, 316
BsaJI CCNNGG 1 cut(s) 901
BsaWI WCCGGW 2 cut(s) 19, 599
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 623
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 2 cut(s) 427, 553
Bse3DI GCAATG 4 cut(s) 540, 561, 614, 875
Bse8I GATNNNNATC 2 cut(s) 280, 316
BseBI CCWGG 2 cut(s) 329, 618
BseCI ATCGAT 1 cut(s) 274
BseDI CCNNGG 1 cut(s) 901
BseGI GGATG 1 cut(s) 37
BseJI GATNNNNATC 2 cut(s) 280, 316
BseLI CCNNNNNNNGG 2 cut(s) 321, 623
BseMI GCAATG 4 cut(s) 540, 561, 614, 875
BseMII CTCAG 1 cut(s) 822
BseNI ACTGG 2 cut(s) 427, 553
BseSI GKGCMC 3 cut(s) 461, 642, 867
BseX3I CGGCCG 1 cut(s) 858
BseXI GCAGC 3 cut(s) 489, 771, 806
BsgI GTGCAG 1 cut(s) 805
Bsh1236I CGCG 2 cut(s) 47, 785
Bsh1285I CGRYCG 2 cut(s) 184, 861
BshFI GGCC 2 cut(s) 71, 860
BshTI ACCGGT 1 cut(s) 599
BshVI ATCGAT 1 cut(s) 274
BsiEI CGRYCG 2 cut(s) 184, 861
BsiHKAI GWGCWC 1 cut(s) 461
BsiSI CCGG 3 cut(s) 20, 600, 867
BsiWI CGTACG 1 cut(s) 711
BslFI GGGAC 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 321, 623
BsmAI GTCTC 1 cut(s) 439
BsmFI GGGAC 1 cut(s) 65
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 2 cut(s) 71, 860
Bsp1286I GDGCHC 3 cut(s) 461, 642, 867
Bsp143I GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
BspACI CCGC 4 cut(s) 180, 203, 612, 783
BspANI GGCC 2 cut(s) 71, 860
BspCNI CTCAG 1 cut(s) 821
BspDI ATCGAT 1 cut(s) 274
BspFNI CGCG 2 cut(s) 47, 785
BspLI GGNNCC 2 cut(s) 313, 326
BspMAI CTGCAG 1 cut(s) 339
BspMI ACCTGC 1 cut(s) 470
BspPI GGATC 7 cut(s) 11, 289, 306, 319, 319, 332, 846
BspQI GCTCTTC 1 cut(s) 155
BspTI CTTAAG 1 cut(s) 850
BsrDI GCAATG 4 cut(s) 540, 561, 614, 875
BsrFI RCCGGY 1 cut(s) 599
BsrI ACTGG 2 cut(s) 427, 553
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 1 cut(s) 901
BssMI GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
BssT1I CCWWGG 1 cut(s) 901
Bst2UI CCWGG 2 cut(s) 329, 618
Bst4CI ACNGT 3 cut(s) 56, 389, 948
Bst6I CTCTTC 1 cut(s) 155
BstAFI CTTAAG 1 cut(s) 850
BstC8I GCNNGC 5 cut(s) 89, 93, 475, 642, 789
BstDEI CTNAG 1 cut(s) 808
BstF5I GGATG 1 cut(s) 37
BstFNI CGCG 2 cut(s) 47, 785
BstHHI GCGC 2 cut(s) 47, 787
BstKTI GATC 8 cut(s) 19, 274, 284, 314, 320, 327, 841, 911
BstMAI GTCTC 1 cut(s) 439
BstMBI GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
BstMCI CGRYCG 2 cut(s) 184, 861
BstMWI GCNNNNNNNGC 1 cut(s) 670
BstNI CCWGG 2 cut(s) 329, 618
BstSCI CCNGG 3 cut(s) 327, 616, 865
BstSFI CTRYAG 1 cut(s) 335
BstSLI GKGCMC 3 cut(s) 461, 642, 867
BstUI CGCG 2 cut(s) 47, 785
BstV1I GCAGC 3 cut(s) 489, 771, 806
BstX2I RGATCY 5 cut(s) 16, 281, 311, 317, 324
BstYI RGATCY 5 cut(s) 16, 281, 311, 317, 324
BstZI CGGCCG 1 cut(s) 858
Bsu15I ATCGAT 1 cut(s) 274
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 2 cut(s) 71, 860
BsuTUI ATCGAT 1 cut(s) 274
BtgZI GCGATG 1 cut(s) 230
BtsCI GGATG 1 cut(s) 37
BtsI GCAGTG 1 cut(s) 738
BtsIMutI CAGTG 2 cut(s) 61, 738
BveI ACCTGC 1 cut(s) 470
Cac8I GCNNGC 5 cut(s) 89, 93, 475, 642, 789
CaiI CAGNNNCTG 1 cut(s) 334
CfoI GCGC 2 cut(s) 47, 787
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 614
ClaI ATCGAT 1 cut(s) 274
CsiI ACCWGGT 1 cut(s) 616
Csp6I GTAC 2 cut(s) 420, 712
CspAI ACCGGT 1 cut(s) 599
CviAII CATG 1 cut(s) 623
CviQI GTAC 2 cut(s) 420, 712
DdeI CTNAG 1 cut(s) 808
DpnI GATC 8 cut(s) 18, 273, 283, 313, 319, 326, 840, 910
DpnII GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
EaeI YGGCCR 2 cut(s) 69, 858
EagI CGGCCG 1 cut(s) 858
Eam1104I CTCTTC 1 cut(s) 155
EarI CTCTTC 1 cut(s) 155
EclXI CGGCCG 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 901
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 614
Eco52I CGGCCG 1 cut(s) 858
EcoRII CCWGG 2 cut(s) 327, 616
EcoRV GATATC 1 cut(s) 654
EcoT14I CCWWGG 1 cut(s) 901
ErhI CCWWGG 1 cut(s) 901
FaeI CATG 1 cut(s) 626
FaqI GGGAC 1 cut(s) 65
FatI CATG 1 cut(s) 622
Fnu4HI GCNGC 4 cut(s) 6, 478, 760, 820
FokI GGATG 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 6, 478, 760, 820
FspBI CTAG 6 cut(s) 132, 138, 269, 365, 645, 941
GlaI GCGC 2 cut(s) 46, 786
GluI GCNGC 4 cut(s) 6, 478, 760, 820
GsuI CTGGAG 1 cut(s) 570
HaeIII GGCC 2 cut(s) 71, 860
HapII CCGG 3 cut(s) 20, 600, 867
HhaI GCGC 2 cut(s) 47, 787
Hin1II CATG 1 cut(s) 626
Hin6I GCGC 2 cut(s) 45, 785
HinP1I GCGC 2 cut(s) 45, 785
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HinfI GANTC 7 cut(s) 197, 233, 276, 289, 299, 533, 688
HpaII CCGG 3 cut(s) 20, 600, 867
HphI GGTGA 3 cut(s) 34, 751, 915
Hpy166II GTNNAC 2 cut(s) 459, 513
Hpy188I TCNGA 2 cut(s) 281, 811
Hpy188III TCNNGA 4 cut(s) 157, 269, 315, 941
Hpy8I GTNNAC 2 cut(s) 459, 513
HpyAV CCTTC 3 cut(s) 19, 730, 761
HpyCH4III ACNGT 3 cut(s) 56, 389, 948
HpyCH4IV ACGT 3 cut(s) 441, 710, 714
HpyF10VI GCNNNNNNNGC 1 cut(s) 670
HpyF3I CTNAG 1 cut(s) 808
HpySE526I ACGT 3 cut(s) 441, 710, 714
Hsp92II CATG 1 cut(s) 626
HspAI GCGC 2 cut(s) 45, 785
Kzo9I GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 1 cut(s) 728
Lsp1109I GCAGC 3 cut(s) 489, 771, 806
MabI ACCWGGT 1 cut(s) 616
MaeI CTAG 6 cut(s) 132, 138, 269, 365, 645, 941
MaeII ACGT 3 cut(s) 441, 710, 714
MaeIII GTNAC 2 cut(s) 350, 748
MalI GATC 8 cut(s) 18, 273, 283, 313, 319, 326, 840, 910
MboI GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
MboII GAAGA 2 cut(s) 142, 918
MfeI CAATTG 2 cut(s) 258, 346
MflI RGATCY 5 cut(s) 16, 281, 311, 317, 324
MhlI GDGCHC 3 cut(s) 461, 642, 867
MluCI AATT 6 cut(s) 119, 258, 346, 450, 567, 699
MlyI GAGTC 2 cut(s) 206, 242
MnlI CCTC 5 cut(s) 22, 33, 95, 108, 569
MseI TTAA 4 cut(s) 453, 570, 702, 851
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 203
MspCI CTTAAG 1 cut(s) 850
MspI CCGG 3 cut(s) 20, 600, 867
MspR9I CCNGG 3 cut(s) 329, 618, 867
MunI CAATTG 2 cut(s) 258, 346
Mva1269I GAATGC 1 cut(s) 880
MvaI CCWGG 2 cut(s) 329, 618
MvnI CGCG 2 cut(s) 47, 785
MwoI GCNNNNNNNGC 1 cut(s) 670
NciI CCSGG 1 cut(s) 867
NdeII GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
NlaIII CATG 1 cut(s) 626
NlaIV GGNNCC 2 cut(s) 313, 326
NmeAIII GCCGAG 1 cut(s) 836
NmuCI GTSAC 1 cut(s) 350
PaqCI CACCTGC 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 155
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 5 cut(s) 276, 289, 299, 533, 688
Pfl23II CGTACG 1 cut(s) 711
PflFI GACNNNGTC 1 cut(s) 618
PflMI CCANNNNNTGG 1 cut(s) 623
PinAI ACCGGT 1 cut(s) 599
PkrI GCNGC 4 cut(s) 7, 479, 761, 821
PleI GAGTC 2 cut(s) 205, 241
PpsI GAGTC 2 cut(s) 205, 241
PsiI TTATAA 2 cut(s) 590, 698
Psp1406I AACGTT 1 cut(s) 441
Psp6I CCWGG 2 cut(s) 327, 616
PspGI CCWGG 2 cut(s) 327, 616
PspLI CGTACG 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 313, 326
PspPI GGNCC 1 cut(s) 614
PstI CTGCAG 1 cut(s) 339
PstNI CAGNNNCTG 1 cut(s) 334
PsuI RGATCY 5 cut(s) 16, 281, 311, 317, 324
PsyI GACNNNGTC 1 cut(s) 618
RsaI GTAC 2 cut(s) 421, 713
RsaNI GTAC 2 cut(s) 420, 712
RseI CAYNNNNRTG 1 cut(s) 833
SapI GCTCTTC 1 cut(s) 155
SaqAI TTAA 4 cut(s) 453, 570, 702, 851
SatI GCNGC 4 cut(s) 6, 478, 760, 820
Sau3AI GATC 8 cut(s) 16, 271, 281, 311, 317, 324, 838, 908
Sau96I GGNCC 1 cut(s) 614
SchI GAGTC 2 cut(s) 206, 242
ScrFI CCNGG 3 cut(s) 329, 618, 867
SduI GDGCHC 3 cut(s) 461, 642, 867
SexAI ACCWGGT 1 cut(s) 616
SfcI CTRYAG 1 cut(s) 335
SinI GGWCC 1 cut(s) 614
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 2 cut(s) 157, 850
SmoI CTYRAG 2 cut(s) 157, 850
Sse9I AATT 6 cut(s) 119, 258, 346, 450, 567, 699
SsiI CCGC 4 cut(s) 180, 203, 612, 783
SspMI CTAG 6 cut(s) 132, 138, 269, 365, 645, 941
StyD4I CCNGG 3 cut(s) 327, 616, 865
StyI CCWWGG 1 cut(s) 901
TaaI ACNGT 3 cut(s) 56, 389, 948
TaiI ACGT 3 cut(s) 444, 713, 717
TaqI TCGA 2 cut(s) 274, 342
TasI AATT 6 cut(s) 119, 258, 346, 450, 567, 699
TatI WGTACW 1 cut(s) 419
TfiI GAWTC 5 cut(s) 276, 289, 299, 533, 688
Tru1I TTAA 4 cut(s) 453, 570, 702, 851
Tru9I TTAA 4 cut(s) 453, 570, 702, 851
TscAI CASTG 2 cut(s) 61, 738
TseFI GTSAC 1 cut(s) 350
TseI GCWGC 4 cut(s) 5, 477, 759, 819
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 3 cut(s) 142, 389, 434
TspRI CASTG 2 cut(s) 61, 738
Tth111I GACNNNGTC 1 cut(s) 618
Van91I CCANNNNNTGG 1 cut(s) 623
Vha464I CTTAAG 1 cut(s) 850
VneI GTGCAC 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 614
XbaI TCTAGA 2 cut(s) 268, 940
XcmI CCANNNNNNNNNTGG 1 cut(s) 832
XspI CTAG 6 cut(s) 132, 138, 269, 365, 645, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.