FvH4_2g29270

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000579
Physical Location & Seq
Forward (+)
22733762 .. 22735903
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29270.t1

Sequence Viewer

Length: 1332 bp
ATGCAGTACATATTAGTGAGCTTTTTGATATTTTACACATATTCATCTCATTTAAGCGAAATTGTATGTGGCCAAACGTTTAGTGTTCTTGATTACGATGCTGTTGGAGATGGAGACACTGATGATTCACAGGCATTTGTAAGAGCATGGGAAGCTGTTTGTGGAGCTGAAACTTACGATGTACCAACACTTCAAATACCGGAGGAGAAAACGTTCTTATTGCAGCCTACGAAATTTAAAGGTCCTTGCAAATCAAATAGGGTTCATGTTCAGGTGTTGGGGAAGCTGGTGGCACCCGAAACACCGGAAGCTTGGAAAGAGTGTGAAACCGATGTCTGGCTCACGTTTACGGATGTGCACAATCTCACTGTCAATGGTTCGGGACAAATCGACGGCCGGGGTTCATCTTGGTGGAGCAAGGCTGAACACCAAAGGCTGTACATAGATGAAAAACATAAGAATGATGATGAGGTGGTGAAGTGCCATCGACCAAAGGCTCTACACTTCCAAGGATGTCATAACCTTCTACTGACTGGACTCACTCATGTCAATAGTCCAAAAGCTCATATTAGTATAAGCAACTGCAGATATGTATATGTCGCCAATCTCACTATAACTGCACCTGAAGATAGTCCAAACACCGATGGAATTGACATCTCAAACTCGAACTATGTCAATATTCATTCCTCTTATATTGGAACTGGTGATGATTGTATCGCTATCAACTCTGGATGTTCTAATCTCAACATTACCACCATCAAGTGTGGCCCAGGTCATGGAATTAGTGTGGGAAGCTTGGGACAAGATGGGGCTTATGCGACGGTGGAAAATGTATACGTGAAAAATTGCAGTTTCAGTGGAACTCAAAATGGAGTAAGAATCAAGACATGGCAGGGAGGGTCTGGGTACGCCAAGAATATAACATTTGAGAAAATCAGACTTCATGCTGCTAAGAACTCAATTATCATCGACCAATATTATTGTAACGGAGGTCATGGTTGCAAAAACAAGAGCTCAGCTGTAGAGATAAGTGATGTGAGGTACATGGATATCAAAGGTACTTCAGCGAAGGAGGATGCGATAATATTCAACTGCAATCAGTTTTCCGGCTGTCGCAACATCGTAATGGACAACATCAACATCACCTCAGATGTTCCCGGAGAGGAGCTATATGCCTCATGCTACAACATCAATGGAACTTCTAACAGCACCTTGCCCTGTGTAACTTGCCTGTCCAATAGTACATCCTCAGCACCGTACCCAGCTCCTCCGCCCTTAGCACCGTACGCAGCTCCTCAGGCGCTCTTAGCACCGTACTCGCCTCTGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

444

Amino Acids

48.29

Weight (kDa)

5.58

Isoelectric Point (pI)

41.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 62 - 398 3e-90 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 292
AccB7I CCANNNNNTGG 1 cut(s) 776
AccI GTMKAC 1 cut(s) 834
AciI CCGC 1 cut(s) 1271
AclI AACGTT 2 cut(s) 77, 212
AcoI YGGCCR 2 cut(s) 70, 394
AcsI RAATTY 1 cut(s) 233
AcuI CTGAAG 2 cut(s) 645, 1047
AfiI CCNNNNNNNGG 2 cut(s) 336, 776
AgsI TTSAA 2 cut(s) 194, 1090
AjnI CCWGG 1 cut(s) 769
AloI GAACNNNNNNTCC 2 cut(s) 197, 229
Alw21I GWGCWC 2 cut(s) 360, 1016
Alw26I GTCTC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 356
AoxI GGCC 3 cut(s) 70, 394, 766
ApaLI GTGCAC 1 cut(s) 356
ApeKI GCWGC 3 cut(s) 223, 947, 1289
ApoI RAATTY 1 cut(s) 233
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 1303
AspS9I GGNCC 2 cut(s) 242, 767
AsuC2I CCSGG 2 cut(s) 398, 1158
AsuHPI GGTGA 3 cut(s) 487, 716, 1135
AvaII GGWCC 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 1296
BaeGI GKGCMC 1 cut(s) 360
BalI TGGCCA 1 cut(s) 72
BanI GGYRCC 1 cut(s) 292
BanII GRGCYC 1 cut(s) 1016
BarI GAAGNNNNNNTAC 2 cut(s) 174, 206
Bbv12I GWGCWC 2 cut(s) 360, 1016
BbvCI CCTCAGC 1 cut(s) 1249
BbvI GCAGC 3 cut(s) 235, 934, 1301
BccI CCATC 5 cut(s) 104, 492, 638, 764, 800
BceAI ACGGC 1 cut(s) 409
BcgI CGANNNNNNTGC 1 cut(s) 1299
BciT130I CCWGG 1 cut(s) 771
BcnI CCSGG 2 cut(s) 398, 1158
BcoDI GTCTC 1 cut(s) 108
BfmI CTRYAG 2 cut(s) 583, 1020
BfoI RGCGCY 1 cut(s) 1304
BisI GCNGC 3 cut(s) 224, 948, 1290
BlpI GCTNAGC 1 cut(s) 1015
BlsI GCNGC 3 cut(s) 225, 949, 1291
Bme1390I CCNGG 3 cut(s) 398, 771, 1158
Bme18I GGWCC 1 cut(s) 242
BmgT120I GGNCC 2 cut(s) 242, 767
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 3 cut(s) 398, 771, 1158
BmsI GCATC 2 cut(s) 88, 1066
Bpu10I CCTNAGC 2 cut(s) 1249, 1276
Bpu1102I GCTNAGC 1 cut(s) 1015
BpuMI CCSGG 2 cut(s) 398, 1158
BsaAI YACGTR 1 cut(s) 838
BsaJI CCNNGG 3 cut(s) 397, 508, 769
BsaWI WCCGGW 2 cut(s) 199, 304
BsaXI ACNNNNNCTCC 2 cut(s) 197, 227
Bsc4I CCNNNNNNNGG 2 cut(s) 336, 776
Bse1I ACTGG 2 cut(s) 538, 706
Bse21I CCTNAGG 1 cut(s) 1296
BseBI CCWGG 1 cut(s) 771
BseDI CCNNGG 3 cut(s) 397, 508, 769
BseGI GGATG 5 cut(s) 358, 518, 737, 1081, 1244
BseLI CCNNNNNNNGG 2 cut(s) 336, 776
BseMII CTCAG 4 cut(s) 1029, 1161, 1263, 1310
BseNI ACTGG 2 cut(s) 538, 706
BseRI GAGGAG 4 cut(s) 218, 1178, 1257, 1284
BseSI GKGCMC 1 cut(s) 360
BseX3I CGGCCG 1 cut(s) 394
BseXI GCAGC 3 cut(s) 235, 934, 1301
BseYI CCCAGC 1 cut(s) 1261
BsgI GTGCAG 1 cut(s) 603
Bsh1285I CGRYCG 1 cut(s) 397
BshFI GGCC 3 cut(s) 72, 396, 768
BshNI GGYRCC 1 cut(s) 292
BsiEI CGRYCG 1 cut(s) 397
BsiHKAI GWGCWC 2 cut(s) 360, 1016
BsiSI CCGG 5 cut(s) 200, 305, 397, 1107, 1158
BsiWI CGTACG 1 cut(s) 1284
BslFI GGGAC 2 cut(s) 396, 813
BslI CCNNNNNNNGG 2 cut(s) 336, 776
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 396, 813
BsnI GGCC 3 cut(s) 72, 396, 768
Bsp1286I GDGCHC 2 cut(s) 360, 1016
Bsp1407I TGTACA 1 cut(s) 438
Bsp143I GATC 1 cut(s) 1326
Bsp1720I GCTNAGC 1 cut(s) 1015
BspACI CCGC 1 cut(s) 1271
BspANI GGCC 3 cut(s) 72, 396, 768
BspCNI CTCAG 4 cut(s) 1028, 1160, 1262, 1309
BspLI GGNNCC 1 cut(s) 294
BspMAI CTGCAG 1 cut(s) 587
BspT107I GGYRCC 1 cut(s) 292
BsrGI TGTACA 1 cut(s) 438
BsrI ACTGG 2 cut(s) 538, 706
BssECI CCNNGG 3 cut(s) 397, 508, 769
BssMI GATC 1 cut(s) 1326
BssNAI GTATAC 1 cut(s) 835
BssT1I CCWWGG 1 cut(s) 508
Bst1107I GTATAC 1 cut(s) 835
Bst2UI CCWGG 1 cut(s) 771
Bst4CI ACNGT 5 cut(s) 370, 823, 1257, 1284, 1314
BstAUI TGTACA 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 838
BstDEI CTNAG 7 cut(s) 951, 1015, 1147, 1249, 1276, 1296, 1306
BstF5I GGATG 5 cut(s) 358, 518, 737, 1081, 1244
BstH2I RGCGCY 1 cut(s) 1304
BstHHI GCGC 1 cut(s) 1303
BstKTI GATC 1 cut(s) 1329
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 1 cut(s) 1326
BstMCI CGRYCG 1 cut(s) 397
BstMWI GCNNNNNNNGC 4 cut(s) 152, 1286, 1298, 1307
BstNI CCWGG 1 cut(s) 771
BstSCI CCNGG 3 cut(s) 396, 769, 1156
BstSFI CTRYAG 2 cut(s) 583, 1020
BstSLI GKGCMC 1 cut(s) 360
BstV1I GCAGC 3 cut(s) 235, 934, 1301
BstZ17I GTATAC 1 cut(s) 835
BstZI CGGCCG 1 cut(s) 394
Bsu36I CCTNAGG 1 cut(s) 1296
BsuRI GGCC 3 cut(s) 72, 396, 768
BtsCI GGATG 5 cut(s) 358, 518, 737, 1081, 1244
BtsIMutI CAGTG 3 cut(s) 117, 366, 862
CfoI GCGC 1 cut(s) 1303
Cfr13I GGNCC 2 cut(s) 242, 767
CviAII CATG 9 cut(s) 147, 266, 545, 776, 888, 944, 995, 1045, 1179
DdeI CTNAG 7 cut(s) 951, 1015, 1147, 1249, 1276, 1296, 1306
DpnI GATC 1 cut(s) 1328
DpnII GATC 1 cut(s) 1326
DraI TTTAAA 1 cut(s) 238
EaeI YGGCCR 2 cut(s) 70, 394
EagI CGGCCG 1 cut(s) 394
EciI GGCGGA 1 cut(s) 1260
Ecl136II GAGCTC 1 cut(s) 1014
EclXI CGGCCG 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 508
Eco24I GRGCYC 1 cut(s) 1016
Eco32I GATATC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 242
Eco52I CGGCCG 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 1014
Eco57I CTGAAG 2 cut(s) 645, 1047
Eco81I CCTNAGG 1 cut(s) 1296
EcoICRI GAGCTC 1 cut(s) 1014
EcoO109I RGGNCCY 1 cut(s) 242
EcoRII CCWGG 1 cut(s) 769
EcoRV GATATC 1 cut(s) 1051
EcoT14I CCWWGG 1 cut(s) 508
EcoT38I GRGCYC 1 cut(s) 1016
ErhI CCWWGG 1 cut(s) 508
FaeI CATG 9 cut(s) 150, 269, 548, 779, 891, 947, 998, 1048, 1182
FaqI GGGAC 2 cut(s) 396, 813
FatI CATG 9 cut(s) 146, 265, 544, 775, 887, 943, 994, 1044, 1178
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 3 cut(s) 224, 948, 1290
FokI GGATG 5 cut(s) 365, 525, 744, 1088, 1231
FriOI GRGCYC 1 cut(s) 1016
Fsp4HI GCNGC 3 cut(s) 224, 948, 1290
GlaI GCGC 1 cut(s) 1302
GluI GCNGC 3 cut(s) 224, 948, 1290
GsaI CCCAGC 1 cut(s) 1265
HaeII RGCGCY 1 cut(s) 1304
HaeIII GGCC 3 cut(s) 72, 396, 768
HapII CCGG 5 cut(s) 200, 305, 397, 1107, 1158
HhaI GCGC 1 cut(s) 1303
Hin1II CATG 9 cut(s) 150, 269, 548, 779, 891, 947, 998, 1048, 1182
Hin6I GCGC 1 cut(s) 1301
HinP1I GCGC 1 cut(s) 1301
HindIII AAGCTT 2 cut(s) 309, 793
HinfI GANTC 3 cut(s) 125, 537, 879
HpaII CCGG 5 cut(s) 200, 305, 397, 1107, 1158
HphI GGTGA 3 cut(s) 487, 716, 1135
Hpy166II GTNNAC 3 cut(s) 348, 358, 835
Hpy188I TCNGA 3 cut(s) 938, 1150, 1326
Hpy188III TCNNGA 4 cut(s) 89, 381, 729, 883
Hpy8I GTNNAC 3 cut(s) 348, 358, 835
Hpy99I CGWCG 2 cut(s) 395, 823
HpyAV CCTTC 2 cut(s) 533, 1063
HpyCH4III ACNGT 5 cut(s) 370, 823, 1257, 1284, 1314
HpyCH4IV ACGT 4 cut(s) 77, 212, 344, 837
HpyCH4V TGCA 9 cut(s) 4, 223, 249, 358, 585, 620, 849, 1002, 1095
HpyF10VI GCNNNNNNNGC 4 cut(s) 152, 1286, 1298, 1307
HpyF3I CTNAG 7 cut(s) 951, 1015, 1147, 1249, 1276, 1296, 1306
HpySE526I ACGT 4 cut(s) 77, 212, 344, 837
Hsp92II CATG 9 cut(s) 150, 269, 548, 779, 891, 947, 998, 1048, 1182
HspAI GCGC 1 cut(s) 1301
Kzo9I GATC 1 cut(s) 1326
LmnI GCTCC 5 cut(s) 164, 414, 1165, 1270, 1297
Lsp1109I GCAGC 3 cut(s) 235, 934, 1301
LweI GCATC 2 cut(s) 88, 1066
MaeII ACGT 4 cut(s) 77, 212, 344, 837
MaeIII GTNAC 2 cut(s) 983, 1222
MalI GATC 1 cut(s) 1328
MboI GATC 1 cut(s) 1326
MboII GAAGA 1 cut(s) 638
MhlI GDGCHC 2 cut(s) 360, 1016
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 6 cut(s) 60, 233, 648, 780, 844, 960
MluNI TGGCCA 1 cut(s) 72
MlyI GAGTC 1 cut(s) 531
MmeI TCCRAC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 212
MscI TGGCCA 1 cut(s) 72
MseI TTAA 2 cut(s) 53, 237
MslI CAYNNNNRTG 4 cut(s) 14, 409, 459, 1124
Msp20I TGGCCA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 1019
MspI CCGG 5 cut(s) 200, 305, 397, 1107, 1158
MspR9I CCNGG 3 cut(s) 398, 771, 1158
MvaI CCWGG 1 cut(s) 771
MwoI GCNNNNNNNGC 4 cut(s) 152, 1286, 1298, 1307
NciI CCSGG 2 cut(s) 398, 1158
NdeII GATC 1 cut(s) 1326
NlaIII CATG 9 cut(s) 150, 269, 548, 779, 891, 947, 998, 1048, 1182
NlaIV GGNNCC 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 125, 879
Pfl23II CGTACG 1 cut(s) 1284
PflMI CCANNNNNTGG 1 cut(s) 776
PfoI TCCNGGA 1 cut(s) 1156
PkrI GCNGC 3 cut(s) 225, 949, 1291
PleI GAGTC 1 cut(s) 531
PpsI GAGTC 1 cut(s) 531
Ppu21I YACGTR 1 cut(s) 838
PpuMI RGGWCCY 1 cut(s) 242
Psp124BI GAGCTC 1 cut(s) 1016
Psp1406I AACGTT 2 cut(s) 77, 212
Psp5II RGGWCCY 1 cut(s) 242
Psp6I CCWGG 1 cut(s) 769
PspFI CCCAGC 1 cut(s) 1261
PspGI CCWGG 1 cut(s) 769
PspLI CGTACG 1 cut(s) 1284
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 2 cut(s) 242, 767
PspPPI RGGWCCY 1 cut(s) 242
PstI CTGCAG 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 1019
RseI CAYNNNNRTG 4 cut(s) 14, 409, 459, 1124
SacI GAGCTC 1 cut(s) 1016
SaqAI TTAA 2 cut(s) 53, 237
SatI GCNGC 3 cut(s) 224, 948, 1290
Sau3AI GATC 1 cut(s) 1326
Sau96I GGNCC 2 cut(s) 242, 767
SchI GAGTC 1 cut(s) 531
ScrFI CCNGG 3 cut(s) 398, 771, 1158
SduI GDGCHC 2 cut(s) 360, 1016
SfaNI GCATC 2 cut(s) 88, 1066
SfcI CTRYAG 2 cut(s) 583, 1020
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 4 cut(s) 14, 409, 459, 1124
Sse9I AATT 6 cut(s) 60, 233, 648, 780, 844, 960
SsiI CCGC 1 cut(s) 1271
SspI AATATT 3 cut(s) 679, 977, 1086
SstI GAGCTC 1 cut(s) 1016
StyD4I CCNGG 3 cut(s) 396, 769, 1156
StyI CCWWGG 1 cut(s) 508
TaaI ACNGT 5 cut(s) 370, 823, 1257, 1284, 1314
TaiI ACGT 4 cut(s) 80, 215, 347, 840
TaqI TCGA 4 cut(s) 390, 487, 665, 969
TasI AATT 6 cut(s) 60, 233, 648, 780, 844, 960
TatI WGTACW 3 cut(s) 6, 438, 1241
TfiI GAWTC 2 cut(s) 125, 879
Tru1I TTAA 2 cut(s) 53, 237
Tru9I TTAA 2 cut(s) 53, 237
TscAI CASTG 3 cut(s) 124, 373, 862
TseI GCWGC 3 cut(s) 223, 947, 1289
TspDTI ATGAA 6 cut(s) 33, 254, 393, 462, 671, 932
TspGWI ACGGA 2 cut(s) 365, 1002
TspRI CASTG 3 cut(s) 124, 373, 862
Van91I CCANNNNNTGG 1 cut(s) 776
VneI GTGCAC 1 cut(s) 356
VpaK11BI GGWCC 1 cut(s) 242
XapI RAATTY 1 cut(s) 233
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.