RchiOBHm_Chr2g0140421

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000579
Physical Location & Seq
Reverse (-)
57867244 .. 57869526
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51085

Sequence Viewer

Length: 1359 bp
ATGCTTAGTTCAAAATCCCTGAACATGAGGAATATACTTGTCAACCTTTTGATGTTTTACTCTATTGCTTCATCAAATTTTAGTTTTGGACATGGGCAAACAACTTTTAATGTGCTGGATTATGGTGCTGTTGGAGATGGCGATACAGATGATTCTGAAGCATTCTTAAAAGCATGGAATGCCTCATGTTCAACCAACGCTAGTAACGGGACACCAACACTAGTAATACCAGCAGAGAGAACATTCCTTTTGCAACCTACCATCTTCTCAGGTTCTTGCAATTCTAACGACGGTATCCGGGTCGAGATTCTGGGGAAAATTGTGGCACCCAAAAATCCAGATGAATGGAAAGGGTGTGTAGAATCTTGGTTATCTTTTCGAAATTTGAGCAACCTCATAATAAATGGAACGGGAGAAATTAATGGCCAAGGTTCACTTTGGTGGAGCAATATTACCACCCAACCTAACAAAGCTCTACACCTTTTCAAATGCAGTAATCTTCAGTTAAGCGGACTCGCACATATTGACAGTCCAAAGGCTCATATTAGCATCAACCGTAGCAATAATGTCACCGTCTCTAATCTTCATATTCGTGCACCTGAAGATAGTCCTAACACGGATGGTATTGACATCTCTGTCTCAACACATGTCAATATTCATGACTCAACAATAGCAACTGGTAATCTTAATTCTCTTTCATGCATGCGTGAATCTACTAATCAACCTTATAAATCATTAACTATATGTGATGTAAGTTCTTGTCAAATAATTTTCTTTTGGGAATTCATGATAACAGGTGATGATTGTATTGCCATTAATAATGGTTCATCTTTTATCAATATCACCAATATTGCATGTGGACCTGGTCATGGAATTAGTGTGGGAAGTTTAGGTGTGAATGAAAGTTACCAGACGGTAGAAGAGATACAAGTACGAAATTGTAGTTTCAACGGTACACAAAATGGAGCGAGAATTAAGACATGGCAGGGTGGTTCTGGATATGCTAGGAAGATAACATTTGAACACATAACACTAATAGCCGCGAAGCATCCTATCATCATTGACCAACATTACTGTAATGGAAAGCACGACTGTAAAAAATCGGCAGTAGAAGTGAGTGATGTCACATATAGTGATTTTCAAGGAACTTCTTCAAGTGAGGAGGCGATTGTGTTTAATTGCACCGAGACACCGGGTTGTACCAACATTATTATGAATCAAATCAATATCACTTCGGCAGTTGCTTATCAGAACATTTACGCCTTTTGTGACAATGCGAATGGAACTTATAGCACAACTGTTCCCCCAGTCCCTTGCTTGAAGACAAATGAAACAATATCTTTTAATCTATATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

452

Amino Acids

49.11

Weight (kDa)

5.42

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 72 - 227 1.1e-29 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 250 - 427 1.1e-48 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 729
AasI GACNNNNNNGTC 1 cut(s) 635
AccB1I GGYRCC 1 cut(s) 325
AccII CGCG 1 cut(s) 1043
AciI CCGC 2 cut(s) 510, 1041
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 3 cut(s) 76, 382, 782
AcuI CTGAAG 3 cut(s) 177, 485, 621
AfaI GTAC 3 cut(s) 933, 955, 1201
AfiI CCNNNNNNNGG 1 cut(s) 869
AflIII ACRYGT 1 cut(s) 646
AgsI TTSAA 8 cut(s) 12, 192, 487, 949, 1022, 1142, 1155, 1321
AhlI ACTAGT 1 cut(s) 220
AjnI CCWGG 1 cut(s) 862
AleI CACNNNNGTG 1 cut(s) 439
AluBI AGCT 1 cut(s) 473
AluI AGCT 1 cut(s) 473
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 3 cut(s) 580, 643, 1181
Alw44I GTGCAC 1 cut(s) 594
AoxI GGCC 1 cut(s) 424
ApaLI GTGCAC 1 cut(s) 594
ApoI RAATTY 3 cut(s) 76, 382, 782
AseI ATTAAT 2 cut(s) 420, 816
Asp700I GAANNNNTTC 1 cut(s) 1150
AspS9I GGNCC 1 cut(s) 860
AsuC2I CCSGG 2 cut(s) 299, 1194
AsuHPI GGTGA 3 cut(s) 562, 809, 835
AsuII TTCGAA 1 cut(s) 379
AvaII GGWCC 1 cut(s) 860
BaeGI GKGCMC 1 cut(s) 598
BaeI ACNNNNGTAYC 2 cut(s) 277, 310
BalI TGGCCA 1 cut(s) 426
BanI GGYRCC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 1328
Bbv12I GWGCWC 1 cut(s) 598
BccI CCATC 3 cut(s) 131, 269, 614
BciT130I CCWGG 1 cut(s) 864
BciVI GTATCC 1 cut(s) 305
BcnI CCSGG 2 cut(s) 299, 1194
BcoDI GTCTC 3 cut(s) 580, 643, 1181
BcuI ACTAGT 1 cut(s) 220
BfaI CTAG 3 cut(s) 201, 221, 1005
BfuI GTATCC 1 cut(s) 305
BisI GCNGC 1 cut(s) 1041
BlsI GCNGC 1 cut(s) 1042
Bme1390I CCNGG 3 cut(s) 299, 864, 1194
Bme18I GGWCC 1 cut(s) 860
BmgT120I GGNCC 1 cut(s) 860
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 3 cut(s) 299, 864, 1194
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 2 cut(s) 558, 1057
BmuI ACTGGG 1 cut(s) 1301
BpiI GAAGAC 1 cut(s) 1328
Bpu14I TTCGAA 1 cut(s) 379
BpuMI CCSGG 2 cut(s) 299, 1194
BsaJI CCNNGG 1 cut(s) 427
Bsc4I CCNNNNNNNGG 1 cut(s) 869
Bse1I ACTGG 2 cut(s) 682, 1307
BseBI CCWGG 1 cut(s) 864
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 2 cut(s) 625, 1048
BseLI CCNNNNNNNGG 1 cut(s) 869
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 2 cut(s) 682, 1307
BseRI GAGGAG 1 cut(s) 1175
BseSI GKGCMC 1 cut(s) 598
Bsh1236I CGCG 1 cut(s) 1043
BshFI GGCC 1 cut(s) 426
BshNI GGYRCC 1 cut(s) 325
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 2 cut(s) 298, 1193
BslFI GGGAC 2 cut(s) 223, 1295
BslI CCNNNNNNNGG 1 cut(s) 869
BsmAI GTCTC 3 cut(s) 580, 643, 1181
BsmBI CGTCTC 1 cut(s) 580
BsmFI GGGAC 2 cut(s) 223, 1295
BsmI GAATGC 2 cut(s) 161, 184
BsnI GGCC 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 598
BspACI CCGC 2 cut(s) 510, 1041
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 1 cut(s) 281
BspFNI CGCG 1 cut(s) 1043
BspHI TCATGA 2 cut(s) 658, 786
BspLI GGNNCC 1 cut(s) 327
BspT104I TTCGAA 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 325
BsrI ACTGG 2 cut(s) 682, 1307
BssECI CCNNGG 1 cut(s) 427
BssT1I CCWWGG 1 cut(s) 427
Bst2UI CCWGG 1 cut(s) 864
Bst4CI ACNGT 9 cut(s) 293, 530, 557, 574, 916, 953, 1076, 1094, 1300
Bst6I CTCTTC 1 cut(s) 915
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 704
BstDEI CTNAG 2 cut(s) 5, 268
BstF5I GGATG 2 cut(s) 625, 1048
BstFNI CGCG 1 cut(s) 1043
BstMAI GTCTC 3 cut(s) 580, 643, 1181
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 1 cut(s) 864
BstNSI RCATGY 3 cut(s) 650, 706, 858
BstSCI CCNGG 3 cut(s) 297, 862, 1192
BstSLI GKGCMC 1 cut(s) 598
BstUI CGCG 1 cut(s) 1043
BstV2I GAAGAC 1 cut(s) 1328
BstXI CCANNNNNNTGG 1 cut(s) 345
BsuI GTATCC 1 cut(s) 305
BsuRI GGCC 1 cut(s) 426
BtsCI GGATG 2 cut(s) 625, 1048
Cac8I GCNNGC 1 cut(s) 704
CciI TCATGA 2 cut(s) 658, 786
Cfr13I GGNCC 1 cut(s) 860
CsiI ACCWGGT 1 cut(s) 862
Csp6I GTAC 3 cut(s) 932, 954, 1200
CviJI RGCY 4 cut(s) 426, 473, 539, 1040
CviKI_1 RGCY 4 cut(s) 426, 473, 539, 1040
CviQI GTAC 3 cut(s) 932, 954, 1200
DdeI CTNAG 2 cut(s) 5, 268
DrdI GACNNNNNNGTC 1 cut(s) 635
DseDI GACNNNNNNGTC 1 cut(s) 635
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Eco130I CCWWGG 1 cut(s) 427
Eco47I GGWCC 1 cut(s) 860
Eco57I CTGAAG 3 cut(s) 177, 485, 621
EcoRI GAATTC 1 cut(s) 782
EcoRII CCWGG 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 427
EcoT22I ATGCAT 1 cut(s) 704
ErhI CCWWGG 1 cut(s) 427
Esp3I CGTCTC 1 cut(s) 580
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 2 cut(s) 223, 1295
Fnu4HI GCNGC 1 cut(s) 1041
FokI GGATG 2 cut(s) 632, 1035
Fsp4HI GCNGC 1 cut(s) 1041
FspBI CTAG 3 cut(s) 201, 221, 1005
GluI GCNGC 1 cut(s) 1041
HaeIII GGCC 1 cut(s) 426
HapII CCGG 2 cut(s) 298, 1193
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 7 cut(s) 152, 307, 362, 513, 662, 710, 1216
HpaII CCGG 2 cut(s) 298, 1193
HphI GGTGA 3 cut(s) 562, 809, 835
Hpy166II GTNNAC 5 cut(s) 43, 434, 596, 860, 956
Hpy188I TCNGA 2 cut(s) 157, 1251
Hpy188III TCNNGA 5 cut(s) 304, 338, 659, 787, 996
Hpy8I GTNNAC 5 cut(s) 43, 434, 596, 860, 956
Hpy99I CGWCG 1 cut(s) 293
HpyCH4III ACNGT 9 cut(s) 293, 530, 557, 574, 916, 953, 1076, 1094, 1300
HpyCH4V TGCA 7 cut(s) 253, 279, 492, 596, 702, 854, 1182
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 2 cut(s) 5, 268
LmnI GCTCC 2 cut(s) 444, 965
LweI GCATC 2 cut(s) 558, 1057
MabI ACCWGGT 1 cut(s) 862
MaeI CTAG 3 cut(s) 201, 221, 1005
MaeIII GTNAC 5 cut(s) 203, 568, 905, 1123, 1268
MboII GAAGA 8 cut(s) 256, 491, 575, 614, 932, 1021, 1143, 1333
MhlI GDGCHC 1 cut(s) 598
MlsI TGGCCA 1 cut(s) 426
MluNI TGGCCA 1 cut(s) 426
MlyI GAGTC 2 cut(s) 507, 656
MmeI TCCRAC 1 cut(s) 112
MnlI CCTC 5 cut(s) 21, 193, 404, 1153, 1156
Mox20I TGGCCA 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 1150
MscI TGGCCA 1 cut(s) 426
MslI CAYNNNNRTG 3 cut(s) 439, 591, 1211
Msp20I TGGCCA 1 cut(s) 426
MspI CCGG 2 cut(s) 298, 1193
MspR9I CCNGG 3 cut(s) 299, 864, 1194
Mva1269I GAATGC 2 cut(s) 161, 184
MvaI CCWGG 1 cut(s) 864
MvnI CGCG 1 cut(s) 1043
MwoI GCNNNNNNNGC 1 cut(s) 179
NciI CCSGG 2 cut(s) 299, 1194
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 3 cut(s) 568, 1123, 1268
NsiI ATGCAT 1 cut(s) 704
NspI RCATGY 3 cut(s) 650, 706, 858
NspV TTCGAA 1 cut(s) 379
OliI CACNNNNGTG 1 cut(s) 439
PaeI GCATGC 1 cut(s) 706
PagI TCATGA 2 cut(s) 658, 786
PciI ACATGT 1 cut(s) 646
PctI GAATGC 2 cut(s) 161, 184
PdmI GAANNNNTTC 1 cut(s) 1150
PfeI GAWTC 5 cut(s) 152, 307, 362, 710, 1216
PflFI GACNNNGTC 1 cut(s) 864
PkrI GCNGC 1 cut(s) 1042
PleI GAGTC 2 cut(s) 507, 656
PpsI GAGTC 2 cut(s) 507, 656
PscI ACATGT 1 cut(s) 646
PshBI ATTAAT 2 cut(s) 420, 816
PsiI TTATAA 1 cut(s) 729
Psp6I CCWGG 1 cut(s) 862
PspGI CCWGG 1 cut(s) 862
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 1 cut(s) 860
PsyI GACNNNGTC 1 cut(s) 864
RsaI GTAC 3 cut(s) 933, 955, 1201
RsaNI GTAC 3 cut(s) 932, 954, 1200
RseI CAYNNNNRTG 3 cut(s) 439, 591, 1211
SatI GCNGC 1 cut(s) 1041
Sau96I GGNCC 1 cut(s) 860
SchI GAGTC 2 cut(s) 507, 656
ScrFI CCNGG 3 cut(s) 299, 864, 1194
SduI GDGCHC 1 cut(s) 598
SexAI ACCWGGT 1 cut(s) 862
SfaNI GCATC 2 cut(s) 558, 1057
SfuI TTCGAA 1 cut(s) 379
SinI GGWCC 1 cut(s) 860
SmiMI CAYNNNNRTG 3 cut(s) 439, 591, 1211
SpeI ACTAGT 1 cut(s) 220
SphI GCATGC 1 cut(s) 706
SsiI CCGC 2 cut(s) 510, 1041
SspI AATATT 3 cut(s) 451, 655, 850
SspMI CTAG 3 cut(s) 201, 221, 1005
StyD4I CCNGG 3 cut(s) 297, 862, 1192
StyI CCWWGG 1 cut(s) 427
TaaI ACNGT 9 cut(s) 293, 530, 557, 574, 916, 953, 1076, 1094, 1300
TaqI TCGA 2 cut(s) 303, 379
TauI GCSGC 1 cut(s) 1043
TfiI GAWTC 5 cut(s) 152, 307, 362, 710, 1216
TseFI GTSAC 3 cut(s) 568, 1123, 1268
Tsp45I GTSAC 3 cut(s) 568, 1123, 1268
TspGWI ACGGA 1 cut(s) 632
Tth111I GACNNNGTC 1 cut(s) 864
VneI GTGCAC 1 cut(s) 594
VpaK11BI GGWCC 1 cut(s) 860
VspI ATTAAT 2 cut(s) 420, 816
XapI RAATTY 3 cut(s) 76, 382, 782
XceI RCATGY 3 cut(s) 650, 706, 858
XmnI GAANNNNTTC 1 cut(s) 1150
XspI CTAG 3 cut(s) 201, 221, 1005
Zsp2I ATGCAT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.