Prupe.4G130600_v2.0.a1

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000579
Physical Location & Seq
Reverse (-)
7217912 .. 7222217
4306 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G130600.1

Sequence Viewer

Length: 2127 bp
ATGAAGAAGATATTTGTTTTGATTTTGATATTCTGGATTGGTTTTGGACATGGCCAAAGAACGTTCAATGTTCTCGACTACGGTGCTACTGGAGATGGAAAAACTGATGATTCCAAGGCTTTTCTAAAAGCATGGGGAGAGTTGTGTGGAGCAGCAGATGAACCTAATGGCATGCCAACACTTGTTATACCAGAGATGAAAGCATTCTTATTGCAACCCATCAAGTTTCGAGGTCCTTGCAACTCAGATGGTGTTCATGTTCAGATCATGGGCAAACTGGTGGCACCTAAAATGCTGGATGGATGGACCGAGTGTGAATCAGATTCAGATTGGCTCCAATTCAAGGATGTGGGCAATCTCGTAGTCGATGGTTCAGGAGAAATCGACGGTCAGGGTTCATCTTGGTGGACCACTCCCAATCAATACGACAGATTATCACGCAAACTCATGATGAACAAGCAGAATTGCAAAGCACCAGATGCTCTGCACTTCCATGGATGCGATAATCTTAAACTAAGTGGACTTACTCATCTAAACAGCGCAAGAGCCCACATTGCTATAAGCGGTTGCAACAATGTGTATGTGTCTCATCTCACCATAACCGCACCTGAAGACAGTCCAAACACTGATGGCATTGACATCTCAAACTCACGCAATGTCTTCATTCAACACTCTGCTATCGGAACCGGCGATGACTGCATAGCCATCAACGGTGGTTGCTCCGATCTCAACATTGCCAACATCGCGTGTGGGCCGGGTCATGGTATTAGTGTGGGAAGCTTGGGAGCGAATGGGGCTCTTGAGACAGTGCAAAATGTGTACGTGAGAGATAGTAGTTTCAGTGGGACTACAAATGGAGCTAGGATCAAGACATGGCAGGGAGGATCGGGGTACGCTAAGAACATAACCTTTGAGAAGATCACACTCGATGCAGCTAAAAATCCCATCATCATTGACCAGTTTTATTGTAATAATGATCACAACTGTAAAAGTCAGGCCTCGGCTCTGTTGGTGGAAGATGTGAAGTACATTGATTTTGAAGGAACATCTGCGAATGAGGAAGCGATCAAATTAGATTGTGACCAGAATTCAGGTTGCCGCAACATTATAATGGACCGCATCAAAATCACCTCGGCGGTTCCTGATAAGAAGATTTATGCTTCTTGCAACAATGCTATTGGAACTTCCATTGGCACCATTGTGCCCAATGTACCTTGCCTCAAGTCAGGGACGGAACCAACTACTTCTCCGCCAGCTATGCCTTTGCCAACTGTGCCTCTGCCAGCTACGCCTCCGCCATCTCCGCCTTTGCCCGTTATGCCTCCGCCGTCTCCGCCTGTGCCCGTTATGCCTCCGCCTGTGCCTGTTATGCCTCCGCCATCTCTGCCTGTGCCCGTTATGCCTCCGCCATCTCCACCTGTGCCCATTATGCCTCCGCCTGTGCCTGTTATGCCTCCGCCATCTCCGCCTGTGCCCGTTATTCCTCCGCCATCTCCGCCTGTGCCCGTTATTCCTCCACCATCTCCGCCTGAGCTCGTTATGCCTCCGCCATCTCCACCTCTGCCGGCTGCACCTCCACTAGTTCTTATTTTGCCAGTTACTCCTCCGCCAGCTCCACCCTTGCCAGATACGCCTCCGCCCCTACCAGCTACTCCTCATCCATCTCCGCTCCTACCGGCCACGCCTCCGCAGTCTCTGCCCTTACCAGCTACGTCTCCGCAATCTCCACCCTTGCCAGCTACACCTCCGCAATCTCCACCCTTGCCAGCTACGCCTCCGCAATCTCCGCCCTTGCCAGCTACGCCTCCGTCATCTCCACCCTTGCCAGCTACGCCTCCGCCTCTACAGGTTATGCCTCCGCCTTTGCTAAATAGGCCTCGCCCAACTCTGCCTTTGCCAACTACACCTCCAGCAGTTCCTCTGCTTACGCCCCTAGCTCCGCTGGTACCAACTCCGCCTCCCCCAACTCCGCCACCAATACCACAAATGTCCTTCACAGGGGAACCAGGAGTGCCAGATATTTACTTCACACCTGGTGCATCTAGTGCTACTAAACTTCATAAGAAACACTATATTTGGTGTCAATTACTGATCGTGTATGTGTTAACAAGACTACTGTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

709

Amino Acids

74.62

Weight (kDa)

5.9

Isoelectric Point (pI)

72.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1109
Acc65I GGTACC 1 cut(s) 1945
AccB1I GGYRCC 3 cut(s) 283, 1193, 1945
AccBSI CCGCTC 1 cut(s) 1669
AccII CGCG 1 cut(s) 746
AclI AACGTT 1 cut(s) 62
AclWI GGATC 2 cut(s) 872, 892
AcoI YGGCCR 2 cut(s) 52, 1677
AcsI RAATTY 1 cut(s) 1087
AcuI CTGAAG 1 cut(s) 630
AdeI CACNNNGTG 1 cut(s) 2036
AfaI GTAC 5 cut(s) 821, 893, 1028, 1212, 1947
AfiI CCNNNNNNNGG 3 cut(s) 343, 761, 1998
AgsI TTSAA 4 cut(s) 67, 343, 668, 1040
AhlI ACTAGT 1 cut(s) 1579
AjnI CCWGG 2 cut(s) 2005, 2032
AleI CACNNNNGTG 1 cut(s) 1199
Alw21I GWGCWC 1 cut(s) 1536
Alw26I GTCTC 5 cut(s) 591, 797, 1335, 1698, 1719
AlwI GGATC 2 cut(s) 872, 892
AlwNI CAGNNNCTG 1 cut(s) 1696
AoxI GGCC 5 cut(s) 52, 752, 996, 1677, 1874
ApeKI GCWGC 3 cut(s) 152, 932, 1568
ApoI RAATTY 1 cut(s) 1087
Asp700I GAANNNNTTC 1 cut(s) 203
Asp718I GGTACC 1 cut(s) 1945
AspLEI GCGC 1 cut(s) 542
AspS9I GGNCC 5 cut(s) 233, 306, 408, 752, 1114
AsuC2I CCSGG 1 cut(s) 756
AsuHPI GGTGA 2 cut(s) 586, 1120
AvaII GGWCC 4 cut(s) 233, 306, 408, 1114
BaeGI GKGCMC 6 cut(s) 1206, 1344, 1395, 1425, 1476, 1506
BalI TGGCCA 1 cut(s) 54
BanI GGYRCC 3 cut(s) 283, 1193, 1945
BanII GRGCYC 3 cut(s) 550, 799, 1536
BbsI GAAGAC 2 cut(s) 618, 652
Bbv12I GWGCWC 1 cut(s) 1536
BbvI GCAGC 3 cut(s) 164, 944, 1555
BceAI ACGGC 1 cut(s) 1312
BcgI CGANNNNNNTGC 4 cut(s) 65, 99, 219, 253
BciT130I CCWGG 2 cut(s) 2007, 2034
BclI TGATCA 1 cut(s) 976
BcnI CCSGG 1 cut(s) 756
BcoDI GTCTC 5 cut(s) 591, 797, 1335, 1698, 1719
BcuI ACTAGT 1 cut(s) 1579
BfaI CTAG 4 cut(s) 861, 1580, 1934, 2043
BfmI CTRYAG 1 cut(s) 1844
BisI GCNGC 4 cut(s) 153, 933, 1099, 1569
BlsI GCNGC 4 cut(s) 154, 934, 1100, 1570
Bme1390I CCNGG 3 cut(s) 756, 2007, 2034
Bme18I GGWCC 4 cut(s) 233, 306, 408, 1114
BmgT120I GGNCC 5 cut(s) 233, 306, 408, 752, 1114
BmiI GGNNCC 8 cut(s) 285, 335, 685, 1140, 1195, 1236, 1947, 2004
BmrFI CCNGG 3 cut(s) 756, 2007, 2034
BmsI GCATC 5 cut(s) 469, 488, 919, 1128, 2048
BpiI GAAGAC 2 cut(s) 618, 652
BpmI CTGGAG 2 cut(s) 111, 1893
Bpu10I CCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 2 cut(s) 821, 1205
BpuMI CCSGG 1 cut(s) 756
BsaAI YACGTR 1 cut(s) 823
BsaJI CCNNGG 4 cut(s) 114, 493, 999, 1131
BsaXI ACNNNNNCTCC 6 cut(s) 1231, 1261, 1891, 1921, 1942, 1972
Bsc4I CCNNNNNNNGG 3 cut(s) 343, 761, 1998
Bse118I RCCGGY 3 cut(s) 686, 1564, 1675
Bse1I ACTGG 4 cut(s) 94, 282, 958, 1595
Bse3DI GCAATG 3 cut(s) 552, 661, 732
BseBI CCWGG 2 cut(s) 2007, 2034
BseDI CCNNGG 4 cut(s) 114, 493, 999, 1131
BseGI GGATG 5 cut(s) 304, 308, 352, 503, 1657
BseLI CCNNNNNNNGG 3 cut(s) 343, 761, 1998
BseMI GCAATG 3 cut(s) 552, 661, 732
BseMII CTCAG 2 cut(s) 258, 1521
BseNI ACTGG 4 cut(s) 94, 282, 958, 1595
BseRI GAGGAG 2 cut(s) 1593, 1644
BseSI GKGCMC 6 cut(s) 1206, 1344, 1395, 1425, 1476, 1506
BseXI GCAGC 3 cut(s) 164, 944, 1555
BsgI GTGCAG 2 cut(s) 470, 1554
Bsh1236I CGCG 1 cut(s) 746
BshFI GGCC 5 cut(s) 54, 754, 998, 1679, 1876
BshNI GGYRCC 3 cut(s) 283, 1193, 1945
BsiHKAI GWGCWC 1 cut(s) 1536
BsiSI CCGG 4 cut(s) 687, 755, 1565, 1676
BslFI GGGAC 2 cut(s) 859, 1243
BslI CCNNNNNNNGG 3 cut(s) 343, 761, 1998
BsmAI GTCTC 5 cut(s) 591, 797, 1335, 1698, 1719
BsmBI CGTCTC 2 cut(s) 1335, 1719
BsmFI GGGAC 2 cut(s) 859, 1243
BsmI GAATGC 1 cut(s) 203
BsnI GGCC 5 cut(s) 54, 754, 998, 1679, 1876
Bsp1286I GDGCHC 9 cut(s) 550, 799, 1206, 1344, 1395, 1425, 1476, 1506, 1536
Bsp143I GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
Bsp19I CCATGG 1 cut(s) 493
BspANI GGCC 5 cut(s) 54, 754, 998, 1679, 1876
BspCNI CTCAG 2 cut(s) 257, 1522
BspFNI CGCG 1 cut(s) 746
BspHI TCATGA 1 cut(s) 447
BspLI GGNNCC 8 cut(s) 285, 335, 685, 1140, 1195, 1236, 1947, 2004
BspPI GGATC 2 cut(s) 872, 892
BspT107I GGYRCC 3 cut(s) 283, 1193, 1945
BsrBI CCGCTC 1 cut(s) 1669
BsrDI GCAATG 3 cut(s) 552, 661, 732
BsrFI RCCGGY 3 cut(s) 686, 1564, 1675
BsrI ACTGG 4 cut(s) 94, 282, 958, 1595
BssAI RCCGGY 3 cut(s) 686, 1564, 1675
BssECI CCNNGG 4 cut(s) 114, 493, 999, 1131
BssMI GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
BssT1I CCWWGG 2 cut(s) 114, 493
Bst2UI CCWGG 2 cut(s) 2007, 2034
Bst4CI ACNGT 8 cut(s) 83, 389, 617, 713, 808, 986, 1273, 2118
BstAPI GCANNNNNTGC 3 cut(s) 479, 1696, 2045
BstBAI YACGTR 1 cut(s) 823
BstC8I GCNNGC 9 cut(s) 173, 1254, 1284, 1566, 1611, 1737, 1767, 1797, 1827
BstDEI CTNAG 4 cut(s) 244, 515, 897, 1530
BstDSI CCRYGG 1 cut(s) 493
BstF5I GGATG 5 cut(s) 304, 308, 352, 503, 1657
BstFNI CGCG 1 cut(s) 746
BstHHI GCGC 1 cut(s) 542
BstKTI GATC 8 cut(s) 267, 727, 867, 887, 921, 979, 1068, 2094
BstMAI GTCTC 5 cut(s) 591, 797, 1335, 1698, 1719
BstMBI GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
BstNI CCWGG 2 cut(s) 2007, 2034
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 3 cut(s) 754, 2005, 2032
BstSFI CTRYAG 1 cut(s) 1844
BstSLI GKGCMC 6 cut(s) 1206, 1344, 1395, 1425, 1476, 1506
BstUI CGCG 1 cut(s) 746
BstV1I GCAGC 3 cut(s) 164, 944, 1555
BstV2I GAAGAC 2 cut(s) 618, 652
BsuRI GGCC 5 cut(s) 54, 754, 998, 1679, 1876
BtgI CCRYGG 1 cut(s) 493
BtgZI GCGATG 2 cut(s) 705, 727
BtsCI GGATG 5 cut(s) 304, 308, 352, 503, 1657
BtsIMutI CAGTG 3 cut(s) 624, 813, 847
Cac8I GCNNGC 9 cut(s) 173, 1254, 1284, 1566, 1611, 1737, 1767, 1797, 1827
CaiI CAGNNNCTG 1 cut(s) 1696
CciI TCATGA 1 cut(s) 447
CfoI GCGC 1 cut(s) 542
Cfr10I RCCGGY 3 cut(s) 686, 1564, 1675
Cfr13I GGNCC 5 cut(s) 233, 306, 408, 752, 1114
CsiI ACCWGGT 1 cut(s) 2032
Csp6I GTAC 5 cut(s) 820, 892, 1027, 1211, 1946
CviAII CATG 9 cut(s) 50, 132, 172, 257, 268, 448, 494, 761, 873
CviQI GTAC 5 cut(s) 820, 892, 1027, 1211, 1946
DdeI CTNAG 4 cut(s) 244, 515, 897, 1530
DpnI GATC 8 cut(s) 266, 726, 866, 886, 920, 978, 1067, 2093
DpnII GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
DraIII CACNNNGTG 1 cut(s) 2036
EaeI YGGCCR 2 cut(s) 52, 1677
Ecl136II GAGCTC 1 cut(s) 1534
Eco130I CCWWGG 2 cut(s) 114, 493
Eco147I AGGCCT 2 cut(s) 998, 1876
Eco24I GRGCYC 3 cut(s) 550, 799, 1536
Eco47I GGWCC 4 cut(s) 233, 306, 408, 1114
Eco53kI GAGCTC 1 cut(s) 1534
Eco57I CTGAAG 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 1534
EcoO109I RGGNCCY 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 1087
EcoRII CCWGG 2 cut(s) 2005, 2032
EcoT14I CCWWGG 2 cut(s) 114, 493
EcoT38I GRGCYC 3 cut(s) 550, 799, 1536
ErhI CCWWGG 2 cut(s) 114, 493
Esp3I CGTCTC 2 cut(s) 1335, 1719
FaeI CATG 9 cut(s) 53, 135, 175, 260, 271, 451, 497, 764, 876
FaqI GGGAC 2 cut(s) 859, 1243
FatI CATG 9 cut(s) 49, 131, 171, 256, 267, 447, 493, 760, 872
FbaI TGATCA 1 cut(s) 976
Fnu4HI GCNGC 4 cut(s) 153, 933, 1099, 1569
FokI GGATG 5 cut(s) 311, 315, 359, 510, 1644
FriOI GRGCYC 3 cut(s) 550, 799, 1536
Fsp4HI GCNGC 4 cut(s) 153, 933, 1099, 1569
FspBI CTAG 4 cut(s) 861, 1580, 1934, 2043
GlaI GCGC 1 cut(s) 541
GluI GCNGC 4 cut(s) 153, 933, 1099, 1569
GsuI CTGGAG 2 cut(s) 111, 1893
HaeIII GGCC 5 cut(s) 54, 754, 998, 1679, 1876
HapII CCGG 4 cut(s) 687, 755, 1565, 1676
HhaI GCGC 1 cut(s) 542
Hin1II CATG 9 cut(s) 53, 135, 175, 260, 271, 451, 497, 764, 876
Hin6I GCGC 1 cut(s) 540
HinP1I GCGC 1 cut(s) 540
HincII GTYRAC 1 cut(s) 2106
HindII GTYRAC 1 cut(s) 2106
HindIII AAGCTT 1 cut(s) 778
HinfI GANTC 3 cut(s) 110, 317, 323
HpaI GTTAAC 1 cut(s) 2106
HpaII CCGG 4 cut(s) 687, 755, 1565, 1676
HphI GGTGA 2 cut(s) 586, 1120
Hpy166II GTNNAC 4 cut(s) 408, 521, 820, 2106
Hpy188I TCNGA 6 cut(s) 247, 264, 322, 328, 683, 724
Hpy188III TCNNGA 7 cut(s) 34, 74, 375, 448, 800, 868, 1142
Hpy8I GTNNAC 4 cut(s) 408, 521, 820, 2106
Hpy99I CGWCG 1 cut(s) 389
HpyAV CCTTC 2 cut(s) 1034, 2002
HpyCH4III ACNGT 8 cut(s) 83, 389, 617, 713, 808, 986, 1273, 2118
HpyCH4IV ACGT 3 cut(s) 62, 822, 1712
HpyF3I CTNAG 4 cut(s) 244, 515, 897, 1530
HpySE526I ACGT 3 cut(s) 62, 822, 1712
Hsp92II CATG 9 cut(s) 53, 135, 175, 260, 271, 451, 497, 764, 876
HspAI GCGC 1 cut(s) 540
KpnI GGTACC 1 cut(s) 1949
KroI GCCGGC 1 cut(s) 1564
KroNI GCCGGC 1 cut(s) 1566
Ksp22I TGATCA 1 cut(s) 976
KspAI GTTAAC 1 cut(s) 2106
Kzo9I GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
LmnI GCTCC 8 cut(s) 149, 339, 725, 785, 857, 1618, 1674, 1942
Lsp1109I GCAGC 3 cut(s) 164, 944, 1555
LweI GCATC 5 cut(s) 469, 488, 919, 1128, 2048
MabI ACCWGGT 1 cut(s) 2032
MaeI CTAG 4 cut(s) 861, 1580, 1934, 2043
MaeII ACGT 3 cut(s) 62, 822, 1712
MaeIII GTNAC 2 cut(s) 1079, 1597
MalI GATC 8 cut(s) 266, 726, 866, 886, 920, 978, 1067, 2093
MbiI CCGCTC 1 cut(s) 1669
MboI GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
MboII GAAGA 7 cut(s) 16, 19, 623, 652, 928, 1028, 1162
MhlI GDGCHC 9 cut(s) 550, 799, 1206, 1344, 1395, 1425, 1476, 1506, 1536
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 5 cut(s) 338, 463, 1070, 1087, 2084
MluNI TGGCCA 1 cut(s) 54
Mox20I TGGCCA 1 cut(s) 54
MroNI GCCGGC 1 cut(s) 1564
MroXI GAANNNNTTC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 54
MseI TTAA 2 cut(s) 510, 2105
MslI CAYNNNNRTG 4 cut(s) 403, 492, 1109, 1199
Msp20I TGGCCA 1 cut(s) 54
MspA1I CMGCKG 1 cut(s) 1942
MspI CCGG 4 cut(s) 687, 755, 1565, 1676
MspR9I CCNGG 3 cut(s) 756, 2007, 2034
Mva1269I GAATGC 1 cut(s) 203
MvaI CCWGG 2 cut(s) 2007, 2034
MvnI CGCG 1 cut(s) 746
NaeI GCCGGC 1 cut(s) 1566
NciI CCSGG 1 cut(s) 756
NcoI CCATGG 1 cut(s) 493
NdeII GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
NgoMIV GCCGGC 1 cut(s) 1564
NlaIII CATG 9 cut(s) 53, 135, 175, 260, 271, 451, 497, 764, 876
NlaIV GGNNCC 8 cut(s) 285, 335, 685, 1140, 1195, 1236, 1947, 2004
NmeAIII GCCGAG 2 cut(s) 980, 1112
NmuCI GTSAC 1 cut(s) 1079
NspI RCATGY 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 1199
PaeI GCATGC 1 cut(s) 175
PagI TCATGA 1 cut(s) 447
PceI AGGCCT 2 cut(s) 998, 1876
PcsI WCGNNNNNNNCGW 1 cut(s) 687
PctI GAATGC 1 cut(s) 203
PdiI GCCGGC 1 cut(s) 1566
PdmI GAANNNNTTC 1 cut(s) 203
PfeI GAWTC 3 cut(s) 110, 317, 323
PkrI GCNGC 4 cut(s) 154, 934, 1100, 1570
Ppu21I YACGTR 1 cut(s) 823
PpuMI RGGWCCY 1 cut(s) 233
PsiI TTATAA 1 cut(s) 1109
Psp124BI GAGCTC 1 cut(s) 1536
Psp1406I AACGTT 1 cut(s) 62
Psp5II RGGWCCY 1 cut(s) 233
Psp6I CCWGG 2 cut(s) 2005, 2032
PspGI CCWGG 2 cut(s) 2005, 2032
PspN4I GGNNCC 8 cut(s) 285, 335, 685, 1140, 1195, 1236, 1947, 2004
PspPI GGNCC 5 cut(s) 233, 306, 408, 752, 1114
PspPPI RGGWCCY 1 cut(s) 233
PstNI CAGNNNCTG 1 cut(s) 1696
RsaI GTAC 5 cut(s) 821, 893, 1028, 1212, 1947
RsaNI GTAC 5 cut(s) 820, 892, 1027, 1211, 1946
RseI CAYNNNNRTG 4 cut(s) 403, 492, 1109, 1199
SacI GAGCTC 1 cut(s) 1536
SaqAI TTAA 2 cut(s) 510, 2105
SatI GCNGC 4 cut(s) 153, 933, 1099, 1569
Sau3AI GATC 8 cut(s) 264, 724, 864, 884, 918, 976, 1065, 2091
Sau96I GGNCC 5 cut(s) 233, 306, 408, 752, 1114
ScrFI CCNGG 3 cut(s) 756, 2007, 2034
SduI GDGCHC 9 cut(s) 550, 799, 1206, 1344, 1395, 1425, 1476, 1506, 1536
SexAI ACCWGGT 1 cut(s) 2032
SfaNI GCATC 5 cut(s) 469, 488, 919, 1128, 2048
SfcI CTRYAG 1 cut(s) 1844
SinI GGWCC 4 cut(s) 233, 306, 408, 1114
SmiMI CAYNNNNRTG 4 cut(s) 403, 492, 1109, 1199
SmlI CTYRAG 2 cut(s) 800, 1220
SmoI CTYRAG 2 cut(s) 800, 1220
SpeI ACTAGT 1 cut(s) 1579
SphI GCATGC 1 cut(s) 175
Sse9I AATT 5 cut(s) 338, 463, 1070, 1087, 2084
SseBI AGGCCT 2 cut(s) 998, 1876
SspMI CTAG 4 cut(s) 861, 1580, 1934, 2043
SstI GAGCTC 1 cut(s) 1536
StuI AGGCCT 2 cut(s) 998, 1876
StyD4I CCNGG 3 cut(s) 754, 2005, 2032
StyI CCWWGG 2 cut(s) 114, 493
TaaI ACNGT 8 cut(s) 83, 389, 617, 713, 808, 986, 1273, 2118
TaiI ACGT 3 cut(s) 65, 825, 1715
TaqI TCGA 5 cut(s) 75, 229, 366, 384, 927
TaqII GACCGA 1 cut(s) 323
TasI AATT 5 cut(s) 338, 463, 1070, 1087, 2084
TatI WGTACW 1 cut(s) 1026
TauI GCSGC 1 cut(s) 1101
TfiI GAWTC 3 cut(s) 110, 317, 323
Tru1I TTAA 2 cut(s) 510, 2105
Tru9I TTAA 2 cut(s) 510, 2105
TscAI CASTG 3 cut(s) 631, 813, 847
TseFI GTSAC 1 cut(s) 1079
TseI GCWGC 3 cut(s) 152, 932, 1568
Tsp45I GTSAC 1 cut(s) 1079
TspDTI ATGAA 8 cut(s) 17, 174, 212, 245, 387, 467, 652, 2048
TspGWI ACGGA 2 cut(s) 1247, 1797
TspRI CASTG 3 cut(s) 631, 813, 847
VpaK11BI GGWCC 4 cut(s) 233, 306, 408, 1114
XapI RAATTY 1 cut(s) 1087
XceI RCATGY 1 cut(s) 175
XmnI GAANNNNTTC 1 cut(s) 203
XspI CTAG 4 cut(s) 861, 1580, 1934, 2043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.