Rh6DG389600

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000579
Physical Location & Seq
Forward (+)
58223832 .. 58234355
10524 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG389600.1

Sequence Viewer

Length: 864 bp
ATGAAGGCTTTTCTGGAGGCCTGGCAAAGAGCGTGTGGTGCCGTTGGAGAAACACAAACCTTAAACATCCCAAAGTGGAGAAAATACAAGGTGAAACCAGTGCGATTCGATGGTCCTTGTAAGTCGGATAACATTGTAATTCAGGTTGATGGAAGCATTGAGTCTCCTTCGACGATTGGTGAGTGGACGGGATGCGAATCGGGAAGCTGGCTAGAGTTCCAAAATGTGAAGGGTCTCACCATCAAGGGAGCAGGAGTTATAGATGGCAAGGGTTCAATTTGGTGGAGTCACAAACCTCGAATAACTAGCTACACAACTGTAATGCGATTAGCTAGTGATGACTGCATTGCACTTAAGAGCGGCTGCTCTTTTGTAAACATAACTCGTGTAGCATGTGGACCAGGTCATGGTATTAGTGTCGGAAGTTTGGGAAGACATCCTAATGATAAAGTTGAAGAAGTGAACGTTAGTAATTGTGTCTTTTACAGAGCTCAGAATGGTGCAAGAATCAAAACAATTAGGGAAGGATCTGATGGGTATATTAGGGCGATTTCGTTTAAGGAAATCAGACTTGTGCAGACTAGAAATCCAATTTTTATTGACCAACACTACTACAGTGGGTGGCACTCTGGCAACGGAGAAATAAAGGTGAGCGACATAACATTCAGGGGTTTCCAAGGAACTTCAGTTTCCGACGCAGCAATAAGACTCGAATGCTCTAACCTGTGGTGCACCAACATTGTAATGGATCATGTCAACATATCTTCAATGATTCCTGGAAAGCCAACCTATTCTTACTGCGAGAATGTCAAGGGAAGATCAAGGTTTACTACACCTGCTGTTCCTTGCCTGGAAAAGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko01100 map00040 map01100
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

287

Amino Acids

31.75

Weight (kDa)

9.01

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 17 - 100 4.3e-10 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 111 - 271 3.8e-42 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
AccB1I GGYRCC 1 cut(s) 38
AccB7I CCANNNNNTGG 1 cut(s) 407
AccBSI CCGCTC 1 cut(s) 360
AciI CCGC 1 cut(s) 360
AclI AACGTT 1 cut(s) 465
AclWI GGATC 2 cut(s) 535, 756
AcuI CTGAAG 1 cut(s) 669
AfiI CCNNNNNNNGG 1 cut(s) 407
AflII CTTAAG 1 cut(s) 353
AgsI TTSAA 3 cut(s) 276, 455, 768
AjnI CCWGG 4 cut(s) 20, 400, 775, 849
AluBI AGCT 4 cut(s) 207, 309, 332, 491
AluI AGCT 4 cut(s) 207, 309, 332, 491
Alw21I GWGCWC 2 cut(s) 493, 734
Alw26I GTCTC 2 cut(s) 168, 239
Alw44I GTGCAC 1 cut(s) 730
AlwI GGATC 2 cut(s) 535, 756
AoxI GGCC 1 cut(s) 18
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 2 cut(s) 363, 698
AspS9I GGNCC 2 cut(s) 113, 398
AsuHPI GGTGA 4 cut(s) 103, 191, 229, 661
AvaII GGWCC 2 cut(s) 113, 398
BaeGI GKGCMC 1 cut(s) 734
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 493
BauI CACGAG 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 439
Bbv12I GWGCWC 2 cut(s) 493, 734
BbvI GCAGC 2 cut(s) 350, 710
BccI CCATC 5 cut(s) 104, 143, 248, 257, 527
BceAI ACGGC 1 cut(s) 26
BciT130I CCWGG 4 cut(s) 22, 402, 777, 851
BcoDI GTCTC 2 cut(s) 168, 239
BfaI CTAG 4 cut(s) 212, 306, 333, 582
BfmI CTRYAG 1 cut(s) 613
BfrI CTTAAG 1 cut(s) 353
BfuAI ACCTGC 1 cut(s) 844
BisI GCNGC 3 cut(s) 361, 364, 699
BlsI GCNGC 3 cut(s) 362, 365, 700
Bme1390I CCNGG 4 cut(s) 22, 402, 777, 851
Bme18I GGWCC 2 cut(s) 113, 398
BmgT120I GGNCC 2 cut(s) 113, 398
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 4 cut(s) 22, 402, 777, 851
BmsI GCATC 1 cut(s) 182
BpiI GAAGAC 1 cut(s) 439
BpmI CTGGAG 1 cut(s) 35
BsaBI GATNNNNATC 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 676
BsaXI ACNNNNNCTCC 2 cut(s) 240, 270
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 98
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 196
BseBI CCWGG 4 cut(s) 22, 402, 777, 851
BseDI CCNNGG 1 cut(s) 676
BseGI GGATG 3 cut(s) 66, 197, 436
BseJI GATNNNNATC 1 cut(s) 196
BseLI CCNNNNNNNGG 1 cut(s) 407
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 1 cut(s) 506
BseNI ACTGG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 2 cut(s) 350, 710
BsgI GTGCAG 1 cut(s) 596
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 493, 734
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 2 cut(s) 168, 239
BsmI GAATGC 1 cut(s) 719
BsnI GGCC 1 cut(s) 20
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 493, 734
Bsp143I GATC 3 cut(s) 527, 748, 818
BspACI CCGC 1 cut(s) 360
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 2 cut(s) 535, 756
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 239
BsrBI CCGCTC 1 cut(s) 360
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 1 cut(s) 98
BssECI CCNNGG 1 cut(s) 676
BssMI GATC 3 cut(s) 527, 748, 818
BssSI CACGAG 1 cut(s) 384
BssT1I CCWWGG 1 cut(s) 676
Bst2BI CACGAG 1 cut(s) 384
Bst2UI CCWGG 4 cut(s) 22, 402, 777, 851
Bst4CI ACNGT 2 cut(s) 319, 617
BstAFI CTTAAG 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 209
BstDEI CTNAG 1 cut(s) 492
BstF5I GGATG 3 cut(s) 66, 197, 436
BstKTI GATC 3 cut(s) 530, 751, 821
BstMAI GTCTC 2 cut(s) 168, 239
BstMBI GATC 3 cut(s) 527, 748, 818
BstMWI GCNNNNNNNGC 1 cut(s) 38
BstNI CCWGG 4 cut(s) 22, 402, 777, 851
BstNSI RCATGY 1 cut(s) 396
BstSCI CCNGG 4 cut(s) 20, 400, 775, 849
BstSFI CTRYAG 1 cut(s) 613
BstSLI GKGCMC 1 cut(s) 734
BstV1I GCAGC 2 cut(s) 350, 710
BstV2I GAAGAC 1 cut(s) 439
BstX2I RGATCY 1 cut(s) 527
BstYI RGATCY 1 cut(s) 527
BsuRI GGCC 1 cut(s) 20
BtsCI GGATG 3 cut(s) 66, 197, 436
BtsIMutI CAGTG 2 cut(s) 105, 622
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 113, 398
CseI GACGC 1 cut(s) 704
CsiI ACCWGGT 1 cut(s) 400
CviAII CATG 3 cut(s) 393, 407, 752
CviJI RGCY 9 cut(s) 8, 20, 207, 211, 309, 332, 363, 491, 784
CviKI_1 RGCY 9 cut(s) 8, 20, 207, 211, 309, 332, 363, 491, 784
DdeI CTNAG 1 cut(s) 492
DpnI GATC 3 cut(s) 529, 750, 820
DpnII GATC 3 cut(s) 527, 748, 818
Ecl136II GAGCTC 1 cut(s) 491
Eco130I CCWWGG 1 cut(s) 676
Eco147I AGGCCT 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 493
Eco31I GGTCTC 1 cut(s) 239
Eco47I GGWCC 2 cut(s) 113, 398
Eco53kI GAGCTC 1 cut(s) 491
Eco57I CTGAAG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 491
EcoRII CCWGG 4 cut(s) 20, 400, 775, 849
EcoT14I CCWWGG 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 676
FaeI CATG 3 cut(s) 396, 410, 755
FaiI YATR 8 cut(s) 260, 380, 394, 408, 540, 659, 753, 761
FatI CATG 3 cut(s) 392, 406, 751
Fnu4HI GCNGC 3 cut(s) 361, 364, 699
FokI GGATG 3 cut(s) 53, 204, 423
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 3 cut(s) 361, 364, 699
FspBI CTAG 4 cut(s) 212, 306, 333, 582
GluI GCNGC 3 cut(s) 361, 364, 699
GsuI CTGGAG 1 cut(s) 35
HaeIII GGCC 1 cut(s) 20
HgaI GACGC 1 cut(s) 704
Hin1II CATG 3 cut(s) 396, 410, 755
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HinfI GANTC 7 cut(s) 105, 161, 197, 286, 507, 708, 772
HphI GGTGA 4 cut(s) 103, 191, 229, 661
Hpy166II GTNNAC 7 cut(s) 186, 376, 398, 463, 732, 757, 828
Hpy188I TCNGA 6 cut(s) 127, 422, 495, 532, 569, 694
Hpy188III TCNNGA 2 cut(s) 14, 201
Hpy8I GTNNAC 7 cut(s) 186, 376, 398, 463, 732, 757, 828
Hpy99I CGWCG 2 cut(s) 175, 698
HpyAV CCTTC 3 cut(s) 177, 223, 518
HpyCH4III ACNGT 2 cut(s) 319, 617
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 5 cut(s) 345, 350, 503, 577, 732
HpyF10VI GCNNNNNNNGC 1 cut(s) 38
HpyF3I CTNAG 1 cut(s) 492
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 3 cut(s) 396, 410, 755
Kzo9I GATC 3 cut(s) 527, 748, 818
LmnI GCTCC 1 cut(s) 248
Lsp1109I GCAGC 2 cut(s) 350, 710
LweI GCATC 1 cut(s) 182
MabI ACCWGGT 1 cut(s) 400
MaeI CTAG 4 cut(s) 212, 306, 333, 582
MaeII ACGT 1 cut(s) 465
MaeIII GTNAC 1 cut(s) 287
MalI GATC 3 cut(s) 529, 750, 820
MbiI CCGCTC 1 cut(s) 360
MboI GATC 3 cut(s) 527, 748, 818
MboII GAAGA 4 cut(s) 444, 467, 756, 828
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 2 cut(s) 493, 734
MluCI AATT 5 cut(s) 138, 276, 472, 516, 591
MlyI GAGTC 3 cut(s) 170, 295, 702
MmeI TCCRAC 4 cut(s) 25, 105, 400, 717
MnlI CCTC 2 cut(s) 10, 306
MseI TTAA 3 cut(s) 62, 354, 558
MslI CAYNNNNRTG 2 cut(s) 441, 743
MspCI CTTAAG 1 cut(s) 353
MspR9I CCNGG 4 cut(s) 22, 402, 777, 851
Mva1269I GAATGC 1 cut(s) 719
MvaI CCWGG 4 cut(s) 22, 402, 777, 851
MwoI GCNNNNNNNGC 1 cut(s) 38
NdeII GATC 3 cut(s) 527, 748, 818
NlaIII CATG 3 cut(s) 396, 410, 755
NlaIV GGNNCC 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 287
NspI RCATGY 1 cut(s) 396
PaqCI CACCTGC 1 cut(s) 844
PceI AGGCCT 1 cut(s) 20
PctI GAATGC 1 cut(s) 719
PfeI GAWTC 4 cut(s) 105, 197, 507, 772
PflFI GACNNNGTC 1 cut(s) 402
PflMI CCANNNNNTGG 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 775
PkrI GCNGC 3 cut(s) 362, 365, 700
PleI GAGTC 3 cut(s) 169, 294, 702
PpsI GAGTC 3 cut(s) 169, 294, 702
Psp124BI GAGCTC 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 4 cut(s) 20, 400, 775, 849
PspGI CCWGG 4 cut(s) 20, 400, 775, 849
PspN4I GGNNCC 1 cut(s) 40
PspPI GGNCC 2 cut(s) 113, 398
PsuI RGATCY 1 cut(s) 527
PsyI GACNNNGTC 1 cut(s) 402
RseI CAYNNNNRTG 2 cut(s) 441, 743
SacI GAGCTC 1 cut(s) 493
SaqAI TTAA 3 cut(s) 62, 354, 558
SatI GCNGC 3 cut(s) 361, 364, 699
Sau3AI GATC 3 cut(s) 527, 748, 818
Sau96I GGNCC 2 cut(s) 113, 398
SchI GAGTC 3 cut(s) 170, 295, 702
ScrFI CCNGG 4 cut(s) 22, 402, 777, 851
SduI GDGCHC 2 cut(s) 493, 734
SexAI ACCWGGT 1 cut(s) 400
SfaNI GCATC 1 cut(s) 182
SfcI CTRYAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 113, 398
SmiMI CAYNNNNRTG 2 cut(s) 441, 743
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 5 cut(s) 138, 276, 472, 516, 591
SseBI AGGCCT 1 cut(s) 20
SsiI CCGC 1 cut(s) 360
SspMI CTAG 4 cut(s) 212, 306, 333, 582
SstI GAGCTC 1 cut(s) 493
StuI AGGCCT 1 cut(s) 20
StyD4I CCNGG 4 cut(s) 20, 400, 775, 849
StyI CCWWGG 1 cut(s) 676
TaaI ACNGT 2 cut(s) 319, 617
TaiI ACGT 1 cut(s) 468
TaqI TCGA 4 cut(s) 108, 170, 298, 711
TasI AATT 5 cut(s) 138, 276, 472, 516, 591
TauI GCSGC 1 cut(s) 363
TfiI GAWTC 4 cut(s) 105, 197, 507, 772
Tru1I TTAA 3 cut(s) 62, 354, 558
Tru9I TTAA 3 cut(s) 62, 354, 558
TscAI CASTG 2 cut(s) 105, 622
TseFI GTSAC 1 cut(s) 287
TseI GCWGC 2 cut(s) 363, 698
Tsp45I GTSAC 1 cut(s) 287
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 651
TspRI CASTG 2 cut(s) 105, 622
Tth111I GACNNNGTC 1 cut(s) 402
Van91I CCANNNNNTGG 1 cut(s) 407
Vha464I CTTAAG 1 cut(s) 353
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 2 cut(s) 113, 398
XceI RCATGY 1 cut(s) 396
XcmI CCANNNNNNNNNTGG 1 cut(s) 742
XspI CTAG 4 cut(s) 212, 306, 333, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.