FvH4_2g29242

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000579
Physical Location & Seq
Forward (+)
22727514 .. 22730055
2542 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g29242.t1

Sequence Viewer

Length: 1212 bp
ATGGAGCGATCCTCAATTACCTTTCTGTTTCTGGTCATTGCTTTATCATCTACTTTTGACATTGGATATGGCCAAAACGGCACTTTCGATGTGCGAGATTTTGGTGCGACTGGGAATGGACTAAATGATGATTCTCGAGCTTTTCTTGATGCCTGGCAAAGAGCATGTGGTGCTGTTGGAGAAATACAAACCCTAAACATCCCAATCGGTAGAAAATACATGGTGAAACCAGTGCAATTCGACGGCCCTTGTAACTCAAAAAATATTGTAATTCAGGTTGATGGAAGCATTGAGGCACCTTCGACGATTGGTGAGTGGACGGGATGCGAATTGGGAAGCTGGCTGGGCTTCCAAAATGTTAAAGGTCTCACCATCAAAGGAGCAGGAGTTATAGATGGCAAGGGTTCAATTTGGTGGAGGCAAAAGCCTCAAATAACTAACTACACGACGGGGACTAATTTTGCAGCACCCGCGGCTCTACGATTCCGCTACTGCGATGAACTTGCCCTTAGTGGATTTACTTCTCTCAACAGCCCGAATAAGCATATCATGATACATGGTTCCAATGGTGTAGATATCTCAAGCGTTCATGTATATGCACCTGAATATAGCCCCAACACTGATGGAATTGTTCTCTATGGGTCGATGCGAGTAAATATATTCAACTCTGTCATTAAAACTGGTGATGACTGCATTGCACTTAAGAGCGGCTGCTCCTTTGTAAACATAAGTCGTGTAGCATGTGGACCAGGTCATGGTATTAGTGTGGGAAGTTTGGGAAGACATCCTAATGATAGAGTTGAAGAAGTGAGCGTTAGGAATGTTGTCTTTTACGGAACTCAGAATGGTGCAAGAATCAAAACATTTAGGGGAGGATGTAATGGGTATATTAGAGGGATTTCGTTCAGTAATATCAGACTTGTACAGACTAGAAATCCAATTATTATTGACCAATACTACTTCAGTCGCTGGCACGCTGGCAACGGAGAGATTAAGGTGAGTGACATAACATTCAGGGGTTTCCGAGGAACATCAGCTTCCAATGAAGCAATAATACTCAAGTGCTCTAACCTGTGGTGCACCAATATTGTAATGGATCATGTCAACATATCTTCAATGATTCCTGGAAGGCCAACCTATTCTTACTGTGAGAATGCGAATGGAAGATCAAGCTTTACTACACCTGATGTTCCTTGCTTGAAAAAGCAGTGA

Protein Analysis

404

Amino Acids

43.96

Weight (kDa)

8.77

Isoelectric Point (pI)

32.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 61 - 388 1e-75 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 295
AccB7I CCANNNNNTGG 1 cut(s) 755
AccBSI CCGCTC 1 cut(s) 708
AccII CGCG 1 cut(s) 473
AciI CCGC 4 cut(s) 471, 473, 487, 708
AclWI GGATC 2 cut(s) 3, 1104
AcoI YGGCCR 1 cut(s) 70
AcuI CTGAAG 1 cut(s) 946
AfaI GTAC 1 cut(s) 924
AfiI CCNNNNNNNGG 1 cut(s) 755
AflII CTTAAG 1 cut(s) 701
AgsI TTSAA 5 cut(s) 408, 664, 803, 1116, 1201
AjnI CCWGG 3 cut(s) 152, 748, 1123
AluBI AGCT 4 cut(s) 140, 339, 1037, 1173
AluI AGCT 4 cut(s) 140, 339, 1037, 1173
Alw21I GWGCWC 2 cut(s) 1067, 1082
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 1078
AlwI GGATC 2 cut(s) 3, 1104
AlwNI CAGNNNCTG 1 cut(s) 969
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 3 cut(s) 70, 244, 1130
ApaLI GTGCAC 1 cut(s) 1078
ApeKI GCWGC 2 cut(s) 464, 711
AspS9I GGNCC 2 cut(s) 245, 746
AsuHPI GGTGA 5 cut(s) 235, 323, 361, 695, 1009
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 1 cut(s) 746
BaeGI GKGCMC 1 cut(s) 1082
BalI TGGCCA 1 cut(s) 72
BanI GGYRCC 1 cut(s) 295
BarI GAAGNNNNNNTAC 2 cut(s) 1038, 1070
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 2 cut(s) 1067, 1082
BbvI GCAGC 2 cut(s) 476, 698
BccI CCATC 4 cut(s) 275, 380, 389, 617
BceAI ACGGC 2 cut(s) 94, 259
BcgI CGANNNNNNTGC 2 cut(s) 485, 519
BciT130I CCWGG 3 cut(s) 154, 750, 1125
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 930
BfrI CTTAAG 1 cut(s) 701
BglI GCCNNNNNGGC 1 cut(s) 78
BisI GCNGC 4 cut(s) 465, 474, 709, 712
BlsI GCNGC 4 cut(s) 466, 475, 710, 713
Bme1390I CCNGG 3 cut(s) 154, 750, 1125
Bme18I GGWCC 1 cut(s) 746
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 2 cut(s) 245, 746
BmiI GGNNCC 2 cut(s) 297, 562
BmrFI CCNGG 3 cut(s) 154, 750, 1125
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 3 cut(s) 139, 314, 636
BmuI ACTGGG 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 787
BplI GAGNNNNNCTC 1 cut(s) 28
BpuEI CTTGAG 2 cut(s) 565, 1043
BsaI GGTCTC 1 cut(s) 371
BsaJI CCNNGG 2 cut(s) 471, 1024
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 755
Bse1I ACTGG 3 cut(s) 115, 230, 685
Bse3DI GCAATG 2 cut(s) 36, 693
BseBI CCWGG 3 cut(s) 154, 750, 1125
BseDI CCNNGG 2 cut(s) 471, 1024
BseGI GGATG 4 cut(s) 198, 329, 784, 881
BseLI CCNNNNNNNGG 1 cut(s) 755
BseMI GCAATG 2 cut(s) 36, 693
BseMII CTCAG 1 cut(s) 854
BseNI ACTGG 3 cut(s) 115, 230, 685
BseSI GKGCMC 1 cut(s) 1082
BseXI GCAGC 2 cut(s) 476, 698
BseYI CCCAGC 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 3 cut(s) 72, 246, 1132
BshNI GGYRCC 1 cut(s) 295
BsiHKAI GWGCWC 2 cut(s) 1067, 1082
BsiHKCI CYCGRG 1 cut(s) 135
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 1 cut(s) 755
BsmAI GTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 1 cut(s) 1159
BsnI GGCC 3 cut(s) 72, 246, 1132
Bso31I GGTCTC 1 cut(s) 371
BsoBI CYCGRG 1 cut(s) 135
Bsp1286I GDGCHC 2 cut(s) 1067, 1082
Bsp1407I TGTACA 1 cut(s) 922
Bsp143I GATC 3 cut(s) 8, 1096, 1166
BspACI CCGC 4 cut(s) 471, 473, 487, 708
BspANI GGCC 3 cut(s) 72, 246, 1132
BspCNI CTCAG 1 cut(s) 853
BspFNI CGCG 1 cut(s) 473
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 2 cut(s) 297, 562
BspPI GGATC 2 cut(s) 3, 1104
BspT107I GGYRCC 1 cut(s) 295
BspTI CTTAAG 1 cut(s) 701
BspTNI GGTCTC 1 cut(s) 371
BsrBI CCGCTC 1 cut(s) 708
BsrDI GCAATG 2 cut(s) 36, 693
BsrGI TGTACA 1 cut(s) 922
BsrI ACTGG 3 cut(s) 115, 230, 685
BssECI CCNNGG 2 cut(s) 471, 1024
BssMI GATC 3 cut(s) 8, 1096, 1166
Bst2UI CCWGG 3 cut(s) 154, 750, 1125
Bst4CI ACNGT 1 cut(s) 1148
BstAFI CTTAAG 1 cut(s) 701
BstAPI GCANNNNNTGC 1 cut(s) 170
BstAUI TGTACA 1 cut(s) 922
BstC8I GCNNGC 4 cut(s) 341, 971, 975, 979
BstDEI CTNAG 2 cut(s) 509, 840
BstDSI CCRYGG 1 cut(s) 471
BstF5I GGATG 4 cut(s) 198, 329, 784, 881
BstFNI CGCG 1 cut(s) 473
BstKTI GATC 3 cut(s) 11, 1099, 1169
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 3 cut(s) 8, 1096, 1166
BstMWI GCNNNNNNNGC 5 cut(s) 78, 170, 345, 470, 473
BstNI CCWGG 3 cut(s) 154, 750, 1125
BstNSI RCATGY 2 cut(s) 168, 744
BstSCI CCNGG 3 cut(s) 152, 748, 1123
BstSLI GKGCMC 1 cut(s) 1082
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 2 cut(s) 476, 698
BstV2I GAAGAC 1 cut(s) 787
BsuRI GGCC 3 cut(s) 72, 246, 1132
BtgI CCRYGG 1 cut(s) 471
BtgZI GCGATG 1 cut(s) 510
BtsCI GGATG 4 cut(s) 198, 329, 784, 881
BtsIMutI CAGTG 2 cut(s) 237, 618
Cac8I GCNNGC 4 cut(s) 341, 971, 975, 979
CaiI CAGNNNCTG 1 cut(s) 969
CciI TCATGA 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 245, 746
Cfr42I CCGCGG 1 cut(s) 474
CsiI ACCWGGT 1 cut(s) 748
Csp6I GTAC 1 cut(s) 923
CviAII CATG 8 cut(s) 165, 220, 550, 557, 590, 741, 755, 1100
CviQI GTAC 1 cut(s) 923
DdeI CTNAG 2 cut(s) 509, 840
DpnI GATC 3 cut(s) 10, 1098, 1168
DpnII GATC 3 cut(s) 8, 1096, 1166
EaeI YGGCCR 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 371
Eco32I GATATC 1 cut(s) 577
Eco47I GGWCC 1 cut(s) 746
Eco57I CTGAAG 1 cut(s) 946
Eco88I CYCGRG 1 cut(s) 135
EcoRII CCWGG 3 cut(s) 152, 748, 1123
EcoRV GATATC 1 cut(s) 577
FaeI CATG 8 cut(s) 168, 223, 553, 560, 593, 744, 758, 1103
FaqI GGGAC 1 cut(s) 466
FatI CATG 8 cut(s) 164, 219, 549, 556, 589, 740, 754, 1099
FauI CCCGC 1 cut(s) 478
Fnu4HI GCNGC 4 cut(s) 465, 474, 709, 712
FokI GGATG 4 cut(s) 185, 336, 771, 888
Fsp4HI GCNGC 4 cut(s) 465, 474, 709, 712
FspBI CTAG 1 cut(s) 930
GluI GCNGC 4 cut(s) 465, 474, 709, 712
GsaI CCCAGC 1 cut(s) 347
HaeIII GGCC 3 cut(s) 72, 246, 1132
Hin1II CATG 8 cut(s) 168, 223, 553, 560, 593, 744, 758, 1103
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 1 cut(s) 1171
HinfI GANTC 4 cut(s) 131, 483, 855, 1120
HphI GGTGA 5 cut(s) 235, 323, 361, 695, 1009
Hpy166II GTNNAC 5 cut(s) 318, 724, 746, 1080, 1105
Hpy188I TCNGA 3 cut(s) 843, 917, 1025
Hpy188III TCNNGA 3 cut(s) 135, 146, 550
Hpy8I GTNNAC 5 cut(s) 318, 724, 746, 1080, 1105
Hpy99I CGWCG 3 cut(s) 245, 307, 451
HpyAV CCTTC 2 cut(s) 309, 1122
HpyCH4III ACNGT 1 cut(s) 1148
HpyCH4V TGCA 7 cut(s) 235, 464, 599, 693, 698, 851, 1080
HpyF10VI GCNNNNNNNGC 5 cut(s) 78, 170, 345, 470, 473
HpyF3I CTNAG 2 cut(s) 509, 840
Hsp92II CATG 8 cut(s) 168, 223, 553, 560, 593, 744, 758, 1103
KspI CCGCGG 1 cut(s) 474
Kzo9I GATC 3 cut(s) 8, 1096, 1166
LmnI GCTCC 3 cut(s) 4, 380, 719
Lsp1109I GCAGC 2 cut(s) 476, 698
LweI GCATC 3 cut(s) 139, 314, 636
MabI ACCWGGT 1 cut(s) 748
MaeI CTAG 1 cut(s) 930
MaeIII GTNAC 2 cut(s) 251, 1001
MalI GATC 3 cut(s) 10, 1098, 1168
MbiI CCGCTC 1 cut(s) 708
MboI GATC 3 cut(s) 8, 1096, 1166
MboII GAAGA 4 cut(s) 792, 815, 1104, 1176
MhlI GDGCHC 2 cut(s) 1067, 1082
MlsI TGGCCA 1 cut(s) 72
MluCI AATT 8 cut(s) 15, 236, 270, 329, 408, 457, 627, 939
MluNI TGGCCA 1 cut(s) 72
MmeI TCCRAC 1 cut(s) 157
MnlI CCTC 7 cut(s) 22, 286, 411, 438, 866, 887, 1019
Mox20I TGGCCA 1 cut(s) 72
MscI TGGCCA 1 cut(s) 72
MseI TTAA 4 cut(s) 360, 675, 702, 993
MslI CAYNNNNRTG 2 cut(s) 594, 789
Msp20I TGGCCA 1 cut(s) 72
MspA1I CMGCKG 1 cut(s) 473
MspCI CTTAAG 1 cut(s) 701
MspR9I CCNGG 3 cut(s) 154, 750, 1125
Mva1269I GAATGC 1 cut(s) 1159
MvaI CCWGG 3 cut(s) 154, 750, 1125
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 5 cut(s) 78, 170, 345, 470, 473
NdeII GATC 3 cut(s) 8, 1096, 1166
NlaIII CATG 8 cut(s) 168, 223, 553, 560, 593, 744, 758, 1103
NlaIV GGNNCC 2 cut(s) 297, 562
NmuCI GTSAC 1 cut(s) 1001
NspI RCATGY 2 cut(s) 168, 744
PaeR7I CTCGAG 1 cut(s) 135
PagI TCATGA 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PctI GAATGC 1 cut(s) 1159
PfeI GAWTC 4 cut(s) 131, 483, 855, 1120
PflFI GACNNNGTC 1 cut(s) 750
PflMI CCANNNNNTGG 1 cut(s) 755
PfoI TCCNGGA 1 cut(s) 1123
PkrI GCNGC 4 cut(s) 466, 475, 710, 713
Psp6I CCWGG 3 cut(s) 152, 748, 1123
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 3 cut(s) 152, 748, 1123
PspN4I GGNNCC 2 cut(s) 297, 562
PspPI GGNCC 2 cut(s) 245, 746
PstNI CAGNNNCTG 1 cut(s) 969
PsyI GACNNNGTC 1 cut(s) 750
RsaI GTAC 1 cut(s) 924
RsaNI GTAC 1 cut(s) 923
RseI CAYNNNNRTG 2 cut(s) 594, 789
SacII CCGCGG 1 cut(s) 474
SaqAI TTAA 4 cut(s) 360, 675, 702, 993
SatI GCNGC 4 cut(s) 465, 474, 709, 712
Sau3AI GATC 3 cut(s) 8, 1096, 1166
Sau96I GGNCC 2 cut(s) 245, 746
ScrFI CCNGG 3 cut(s) 154, 750, 1125
SduI GDGCHC 2 cut(s) 1067, 1082
SexAI ACCWGGT 1 cut(s) 748
SfaNI GCATC 3 cut(s) 139, 314, 636
Sfr274I CTCGAG 1 cut(s) 135
Sfr303I CCGCGG 1 cut(s) 474
SgrBI CCGCGG 1 cut(s) 474
SinI GGWCC 1 cut(s) 746
SlaI CTCGAG 1 cut(s) 135
SmiMI CAYNNNNRTG 2 cut(s) 594, 789
SmlI CTYRAG 4 cut(s) 135, 580, 701, 1058
SmoI CTYRAG 4 cut(s) 135, 580, 701, 1058
Sse9I AATT 8 cut(s) 15, 236, 270, 329, 408, 457, 627, 939
SsiI CCGC 4 cut(s) 471, 473, 487, 708
SspI AATATT 2 cut(s) 265, 1087
SspMI CTAG 1 cut(s) 930
StyD4I CCNGG 3 cut(s) 152, 748, 1123
TaaI ACNGT 1 cut(s) 1148
TaqI TCGA 5 cut(s) 87, 136, 240, 302, 644
TasI AATT 8 cut(s) 15, 236, 270, 329, 408, 457, 627, 939
TatI WGTACW 1 cut(s) 922
TauI GCSGC 2 cut(s) 476, 711
TfiI GAWTC 4 cut(s) 131, 483, 855, 1120
Tru1I TTAA 4 cut(s) 360, 675, 702, 993
Tru9I TTAA 4 cut(s) 360, 675, 702, 993
TscAI CASTG 2 cut(s) 237, 625
TseFI GTSAC 1 cut(s) 1001
TseI GCWGC 2 cut(s) 464, 711
Tsp45I GTSAC 1 cut(s) 1001
TspDTI ATGAA 3 cut(s) 513, 578, 1059
TspGWI ACGGA 2 cut(s) 849, 999
TspRI CASTG 2 cut(s) 237, 625
Tth111I GACNNNGTC 1 cut(s) 750
Van91I CCANNNNNTGG 1 cut(s) 755
Vha464I CTTAAG 1 cut(s) 701
VneI GTGCAC 1 cut(s) 1078
VpaK11BI GGWCC 1 cut(s) 746
XceI RCATGY 2 cut(s) 168, 744
XcmI CCANNNNNNNNNTGG 1 cut(s) 1090
XhoI CTCGAG 1 cut(s) 135
XspI CTAG 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.