Rw2G033880

Glycosyl hydrolases family 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000579
Physical Location & Seq
Reverse (-)
55535149 .. 55538133
2985 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033880.1

Sequence Viewer

Length: 1245 bp
ATGCCACGAGGACGCGCCGTTAGACGGAATATACTTGTCAAACTTTTGATGTTTTACTCTATTGCTTCATCAAATTTTAGTTTTGGACATGGGCAAACAACTTTTAATGTGCTGGATTATGGTGCTGTTGGAGATGGCGATACAGATGATTCTGAAGCATTCTTAAAAGCATGGAATGCCTCATGTTCAACCAACGCTAGTAACGGGACACCAACACTAGTAATACCAGCAGAGAGAACATTCCTTTTGCAACCTACCATCTTCTCAGGTTCTTGCAATTCTAACGACGGTATCCGGGTCGAGATTCTGGGGAAAATTGTGGCACCCAAAAATCCAGATGAATGGAAAGGGTGTGTAGAATCTTGGTTATCTTTTGGAAATTTGAGCAACCTCATAATAAATGGAACGGGAGAAATTAATGGCCAAGGTTCACTTTGGTGGAGCAATATTACCACCCAACCTAACAAAGCTCTACACCTTTTCAAATGCAGTAATCTTCAGTTAAGCGGACTCGCACATATTGACAGTCCAAAGGCTCATATTAGCATCAACCGTAGCAATAATGTCACCGTCTCTAATCTTCATATTCGTGCACCTGAAGATAGTCCTAACACGGATGGTATTGACATCTCTGTCTCAACACATGTCAATATTCATGACTCAACAATAGCAACTGGTGATGATTGTATTGCCATTAATAATGGTTCATCTTTTATCAATATCACCAATATTGCATGTGGACCTGGTCATGGAATTAGTGTGGGAAGTTTAGGTGTGAATGAAAGTTATCAGACGGTAGAAGAGATACAAGTACGAAATTGTAGTTTCAACGGTACACAAAATGGAGCGAGAATTAAGACATGGCAGGGTGGTTCTGGATATGCTAGGAAGATAACATTTGAACACATAACACTAATAGCCGCGAAGCATCCTATCATCATTGACCAACATTACTGTAATGGAAAGCACGACTGTAAAAAATCGACTCAGGCAGTAGAAGTGAGTGATGTCACATATAGTGATTTTCAAGGAACTTCTTCAAGTGAGGAGGCGATTGTGTTTAATTGCACCGAGACACCGGGTTGTACCAACATTATTATGAATCAAATCAATATCACTTCGGCAGTTGCTTCTCAGAACATTTATGCCTTTTGTGACAATGCGAATGGAACTTATAGCACAACTGTTCCCCCAGTCCCTTGCTTGAAGACAAATGAAACAATATCTTTTAATCTATATACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28

Protein Analysis

414

Amino Acids

44.62

Weight (kDa)

5.71

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pect-lyase_RHGA_epim PF12708 35 - 282 1.9e-08 Rhamnogalacturonase A/epimerase, pectate lyase-like
Glyco_hydro_28 PF00295 71 - 389 3.2e-85 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000579)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G32370 AT4G32375 AT4G32380 AT4G32380
fragaria_vesca FvH4_2g29242 FvH4_2g29270 FvH4_4g07180 FvH4_4g07190 FvH4_6g32320 FvH4_6g35100
malus_domestica MD03G1260700.v1.1 MD03G1260800.v1.1 MD09G1203400.v1.1 MD15G1065500.v1.1 MD15G1124200.v1.1 MD15G1124300.v1.1
prunus_persica Prupe.1G247300_v2.0.a1 Prupe.1G247300_v2.0.a1 Prupe.1G479600_v2.0.a1 Prupe.1G479700_v2.0.a1 Prupe.3G043600_v2.0.a1 Prupe.4G130600_v2.0.a1 Prupe.7G064200_v2.0.a1
pyrus_communis pycom03g20830 pycom15g06200
rosa_chinensis RchiOBHm_Chr2g0140421 RchiOBHm_Chr2g0146281 RchiOBHm_Chr3g0458021 RchiOBHm_Chr3g0481551 RchiOBHm_Chr4g0399361 RchiOBHm_Chr4g0399381 RchiOBHm_Chr4g0399391 RchiOBHm_Chr4g0399401 RchiOBHm_Chr4g0399411 RchiOBHm_Chr4g0399471
rosa_laevigata RLG00000009265 RLG00000009272 RLG00000011536 RLG00000011537 RLG00000019863 RLG00000020193 RLG00000023386
rosa_multiflora Rmu_sc0000384.1_g000011 Rmu_sc0002800.1_g000011 Rmu_sc0002800.1_g000012 Rmu_sc0017309.1_g000001 Rmu_sc0022824.1_g000003
rosa_roxburghii Rroxscaffold_153G00436780 Rroxscaffold_2G00100180 Rroxscaffold_5G00344320 Rroxscaffold_5G00344370 Rroxscaffold_6G00400330 Rroxscaffold_6G00421350 Rroxscaffold_7G00169940 Rroxscaffold_7G00169950
rosa_rugosa Rorug02G0362600 Rorug02G0394100 Rorug03G0024300 Rorug03G0192500 Rorug04G0016900 Rorug04G0017100 Rorug06G0278700
rosa_samantha Rh2CG399600 Rh2CG437200 Rh3AG084800 Rh3BG087600 Rh3CG088100 Rh3DG088500 Rh3DG270800 Rh4BG091900 Rh4BG092300 Rh5AG287300 Rh6DG389600
rosa_wichuraiana Rw0G012400 Rw2G033880 Rw2G036760 Rw3G007220 Rw3G021790 Rw4G007750 Rw4G007770 Rw4G007810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
AccB1I GGYRCC 1 cut(s) 322
AccII CGCG 2 cut(s) 15, 923
AciI CCGC 2 cut(s) 507, 921
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 73, 379
AcuI CTGAAG 3 cut(s) 174, 482, 618
AfaI GTAC 3 cut(s) 813, 835, 1087
AfiI CCNNNNNNNGG 2 cut(s) 24, 749
AflIII ACRYGT 1 cut(s) 643
AgsI TTSAA 7 cut(s) 189, 484, 829, 902, 1028, 1041, 1207
AhlI ACTAGT 1 cut(s) 217
AjnI CCWGG 1 cut(s) 742
AleI CACNNNNGTG 1 cut(s) 436
AluBI AGCT 1 cut(s) 470
AluI AGCT 1 cut(s) 470
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 3 cut(s) 577, 640, 1067
Alw44I GTGCAC 1 cut(s) 591
AoxI GGCC 1 cut(s) 421
ApaLI GTGCAC 1 cut(s) 591
ApoI RAATTY 2 cut(s) 73, 379
AseI ATTAAT 2 cut(s) 417, 696
Asp700I GAANNNNTTC 1 cut(s) 1036
AspLEI GCGC 1 cut(s) 17
AspS9I GGNCC 1 cut(s) 740
AsuC2I CCSGG 2 cut(s) 296, 1080
AsuHPI GGTGA 3 cut(s) 559, 689, 715
AvaII GGWCC 1 cut(s) 740
BaeGI GKGCMC 1 cut(s) 595
BaeI ACNNNNGTAYC 2 cut(s) 274, 307
BalI TGGCCA 1 cut(s) 423
BanI GGYRCC 1 cut(s) 322
BauI CACGAG 1 cut(s) 6
BbsI GAAGAC 1 cut(s) 1214
Bbv12I GWGCWC 1 cut(s) 595
BccI CCATC 3 cut(s) 128, 266, 611
BceAI ACGGC 1 cut(s) 2
BciT130I CCWGG 1 cut(s) 744
BciVI GTATCC 1 cut(s) 302
BcnI CCSGG 2 cut(s) 296, 1080
BcoDI GTCTC 3 cut(s) 577, 640, 1067
BcuI ACTAGT 1 cut(s) 217
BfaI CTAG 3 cut(s) 198, 218, 885
BfuI GTATCC 1 cut(s) 302
BisI GCNGC 1 cut(s) 921
BlsI GCNGC 1 cut(s) 922
Bme1390I CCNGG 3 cut(s) 296, 744, 1080
Bme18I GGWCC 1 cut(s) 740
BmgT120I GGNCC 1 cut(s) 740
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 3 cut(s) 296, 744, 1080
BmrI ACTGGG 1 cut(s) 1187
BmsI GCATC 2 cut(s) 555, 937
BmuI ACTGGG 1 cut(s) 1187
BpiI GAAGAC 1 cut(s) 1214
BpuMI CCSGG 2 cut(s) 296, 1080
BsaJI CCNNGG 1 cut(s) 424
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 749
Bse1I ACTGG 2 cut(s) 679, 1193
BseBI CCWGG 1 cut(s) 744
BseDI CCNNGG 1 cut(s) 424
BseGI GGATG 2 cut(s) 622, 928
BseLI CCNNNNNNNGG 2 cut(s) 24, 749
BseMII CTCAG 3 cut(s) 279, 1001, 1148
BseNI ACTGG 2 cut(s) 679, 1193
BseRI GAGGAG 1 cut(s) 1061
BseSI GKGCMC 1 cut(s) 595
Bsh1236I CGCG 2 cut(s) 15, 923
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 322
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 2 cut(s) 295, 1079
BslFI GGGAC 2 cut(s) 220, 1181
BslI CCNNNNNNNGG 2 cut(s) 24, 749
BsmAI GTCTC 3 cut(s) 577, 640, 1067
BsmBI CGTCTC 1 cut(s) 577
BsmFI GGGAC 2 cut(s) 220, 1181
BsmI GAATGC 2 cut(s) 158, 181
BsnI GGCC 1 cut(s) 423
Bsp1286I GDGCHC 1 cut(s) 595
BspACI CCGC 2 cut(s) 507, 921
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 3 cut(s) 278, 1000, 1147
BspFNI CGCG 2 cut(s) 15, 923
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 322
BsrI ACTGG 2 cut(s) 679, 1193
BssECI CCNNGG 1 cut(s) 424
BssSI CACGAG 1 cut(s) 6
BssT1I CCWWGG 1 cut(s) 424
Bst2BI CACGAG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 744
Bst4CI ACNGT 9 cut(s) 290, 527, 554, 571, 796, 833, 956, 974, 1186
Bst6I CTCTTC 1 cut(s) 795
BstAPI GCANNNNNTGC 1 cut(s) 176
BstDEI CTNAG 3 cut(s) 265, 987, 1134
BstF5I GGATG 2 cut(s) 622, 928
BstFNI CGCG 2 cut(s) 15, 923
BstHHI GCGC 1 cut(s) 17
BstMAI GTCTC 3 cut(s) 577, 640, 1067
BstMWI GCNNNNNNNGC 1 cut(s) 176
BstNI CCWGG 1 cut(s) 744
BstNSI RCATGY 2 cut(s) 647, 738
BstSCI CCNGG 3 cut(s) 294, 742, 1078
BstSLI GKGCMC 1 cut(s) 595
BstUI CGCG 2 cut(s) 15, 923
BstV2I GAAGAC 1 cut(s) 1214
BstXI CCANNNNNNTGG 1 cut(s) 342
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 1 cut(s) 423
BtsCI GGATG 2 cut(s) 622, 928
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 17
Cfr13I GGNCC 1 cut(s) 740
CseI GACGC 1 cut(s) 21
CsiI ACCWGGT 1 cut(s) 742
Csp6I GTAC 3 cut(s) 812, 834, 1086
CviAII CATG 8 cut(s) 89, 171, 183, 644, 656, 735, 749, 861
CviJI RGCY 4 cut(s) 423, 470, 536, 920
CviKI_1 RGCY 4 cut(s) 423, 470, 536, 920
CviQI GTAC 3 cut(s) 812, 834, 1086
DdeI CTNAG 3 cut(s) 265, 987, 1134
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 1 cut(s) 795
EarI CTCTTC 1 cut(s) 795
Eco130I CCWWGG 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 740
Eco57I CTGAAG 3 cut(s) 174, 482, 618
EcoRII CCWGG 1 cut(s) 742
EcoT14I CCWWGG 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 424
Esp3I CGTCTC 1 cut(s) 577
FaeI CATG 8 cut(s) 92, 174, 186, 647, 659, 738, 752, 864
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FaqI GGGAC 2 cut(s) 220, 1181
FatI CATG 8 cut(s) 88, 170, 182, 643, 655, 734, 748, 860
Fnu4HI GCNGC 1 cut(s) 921
FokI GGATG 2 cut(s) 629, 915
Fsp4HI GCNGC 1 cut(s) 921
FspBI CTAG 3 cut(s) 198, 218, 885
GlaI GCGC 1 cut(s) 16
GluI GCNGC 1 cut(s) 921
HaeIII GGCC 1 cut(s) 423
HapII CCGG 2 cut(s) 295, 1079
HgaI GACGC 1 cut(s) 21
HhaI GCGC 1 cut(s) 17
Hin1II CATG 8 cut(s) 92, 174, 186, 647, 659, 738, 752, 864
Hin6I GCGC 1 cut(s) 15
HinP1I GCGC 1 cut(s) 15
HinfI GANTC 7 cut(s) 149, 304, 359, 510, 659, 985, 1102
HpaII CCGG 2 cut(s) 295, 1079
HphI GGTGA 3 cut(s) 559, 689, 715
Hpy166II GTNNAC 4 cut(s) 431, 593, 740, 836
Hpy188I TCNGA 3 cut(s) 154, 792, 1137
Hpy188III TCNNGA 4 cut(s) 301, 335, 656, 876
Hpy8I GTNNAC 4 cut(s) 431, 593, 740, 836
Hpy99I CGWCG 1 cut(s) 290
HpyCH4III ACNGT 9 cut(s) 290, 527, 554, 571, 796, 833, 956, 974, 1186
HpyCH4V TGCA 6 cut(s) 250, 276, 489, 593, 734, 1068
HpyF10VI GCNNNNNNNGC 1 cut(s) 176
HpyF3I CTNAG 3 cut(s) 265, 987, 1134
Hsp92II CATG 8 cut(s) 92, 174, 186, 647, 659, 738, 752, 864
HspAI GCGC 1 cut(s) 15
LmnI GCTCC 2 cut(s) 441, 845
LweI GCATC 2 cut(s) 555, 937
MabI ACCWGGT 1 cut(s) 742
MaeI CTAG 3 cut(s) 198, 218, 885
MaeIII GTNAC 4 cut(s) 200, 565, 1009, 1154
MboII GAAGA 8 cut(s) 253, 488, 572, 611, 812, 901, 1029, 1219
MhlI GDGCHC 1 cut(s) 595
MlsI TGGCCA 1 cut(s) 423
MluCI AATT 9 cut(s) 73, 277, 315, 379, 414, 753, 817, 852, 1063
MluNI TGGCCA 1 cut(s) 423
MlyI GAGTC 3 cut(s) 504, 653, 979
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 4 cut(s) 190, 401, 1039, 1042
Mox20I TGGCCA 1 cut(s) 423
MroXI GAANNNNTTC 1 cut(s) 1036
MscI TGGCCA 1 cut(s) 423
MseI TTAA 8 cut(s) 105, 164, 417, 503, 696, 855, 1062, 1230
MslI CAYNNNNRTG 3 cut(s) 436, 588, 1097
Msp20I TGGCCA 1 cut(s) 423
MspI CCGG 2 cut(s) 295, 1079
MspR9I CCNGG 3 cut(s) 296, 744, 1080
Mva1269I GAATGC 2 cut(s) 158, 181
MvaI CCWGG 1 cut(s) 744
MvnI CGCG 2 cut(s) 15, 923
MwoI GCNNNNNNNGC 1 cut(s) 176
NciI CCSGG 2 cut(s) 296, 1080
NlaIII CATG 8 cut(s) 92, 174, 186, 647, 659, 738, 752, 864
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 3 cut(s) 565, 1009, 1154
NspI RCATGY 2 cut(s) 647, 738
OliI CACNNNNGTG 1 cut(s) 436
PagI TCATGA 1 cut(s) 655
PciI ACATGT 1 cut(s) 643
PctI GAATGC 2 cut(s) 158, 181
PdmI GAANNNNTTC 1 cut(s) 1036
PfeI GAWTC 4 cut(s) 149, 304, 359, 1102
PflFI GACNNNGTC 1 cut(s) 744
PkrI GCNGC 1 cut(s) 922
PleI GAGTC 3 cut(s) 504, 653, 979
PpsI GAGTC 3 cut(s) 504, 653, 979
PscI ACATGT 1 cut(s) 643
PshBI ATTAAT 2 cut(s) 417, 696
Psp6I CCWGG 1 cut(s) 742
PspGI CCWGG 1 cut(s) 742
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 740
PsyI GACNNNGTC 1 cut(s) 744
RsaI GTAC 3 cut(s) 813, 835, 1087
RsaNI GTAC 3 cut(s) 812, 834, 1086
RseI CAYNNNNRTG 3 cut(s) 436, 588, 1097
SaqAI TTAA 8 cut(s) 105, 164, 417, 503, 696, 855, 1062, 1230
SatI GCNGC 1 cut(s) 921
Sau96I GGNCC 1 cut(s) 740
SchI GAGTC 3 cut(s) 504, 653, 979
ScrFI CCNGG 3 cut(s) 296, 744, 1080
SduI GDGCHC 1 cut(s) 595
SexAI ACCWGGT 1 cut(s) 742
SfaNI GCATC 2 cut(s) 555, 937
SinI GGWCC 1 cut(s) 740
SmiMI CAYNNNNRTG 3 cut(s) 436, 588, 1097
SpeI ACTAGT 1 cut(s) 217
Sse9I AATT 9 cut(s) 73, 277, 315, 379, 414, 753, 817, 852, 1063
SsiI CCGC 2 cut(s) 507, 921
SspI AATATT 3 cut(s) 448, 652, 730
SspMI CTAG 3 cut(s) 198, 218, 885
StyD4I CCNGG 3 cut(s) 294, 742, 1078
StyI CCWWGG 1 cut(s) 424
TaaI ACNGT 9 cut(s) 290, 527, 554, 571, 796, 833, 956, 974, 1186
TaqI TCGA 2 cut(s) 300, 983
TasI AATT 9 cut(s) 73, 277, 315, 379, 414, 753, 817, 852, 1063
TauI GCSGC 1 cut(s) 923
TfiI GAWTC 4 cut(s) 149, 304, 359, 1102
Tru1I TTAA 8 cut(s) 105, 164, 417, 503, 696, 855, 1062, 1230
Tru9I TTAA 8 cut(s) 105, 164, 417, 503, 696, 855, 1062, 1230
TseFI GTSAC 3 cut(s) 565, 1009, 1154
Tsp45I GTSAC 3 cut(s) 565, 1009, 1154
TspDTI ATGAA 8 cut(s) 57, 354, 572, 644, 696, 795, 1115, 1230
TspGWI ACGGA 2 cut(s) 40, 629
Tth111I GACNNNGTC 1 cut(s) 744
VneI GTGCAC 1 cut(s) 591
VpaK11BI GGWCC 1 cut(s) 740
VspI ATTAAT 2 cut(s) 417, 696
XapI RAATTY 2 cut(s) 73, 379
XceI RCATGY 2 cut(s) 647, 738
XmnI GAANNNNTTC 1 cut(s) 1036
XspI CTAG 3 cut(s) 198, 218, 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.